• Parasites, Hosts and Diseases
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• v.43 no.1 s.133
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• pp.19-25
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• 2005

Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against N. caninum infection was evaluated in vitro and in vivo. Two major immunodominant surface antigens (NcSAG1 and NcSRS2) and two dense granule proteins (NcDG1 and NcDG2) of N. caninum tachyzoites were expressed in E. coli, respectively. An in vitro neutralization assay using polyclonal antisera raised against each recombinant antigen showed inhibitory effects on the invasion of N. caninum tachyzoites into host cells. Separate groups of gerbils were immunized with the purified recombinant proteins singly or in combinations and animals were then challenged with N. caninum. Following these experimental challenges, the protective efficacy of each vaccination was determined by assessing animal survival rate. All experimental groups showed protective effects of different degrees against experimental infection. The highest protection efficacy was observed for combined vaccination with NcSRS2 and NcDG1. Our results indicate that combined vaccination with the N. caninum recombinant antigens, NcSRS2 and NcDG1, induces the highest protective effect against N. caninum infection in vitro and in vivo.

• Fire Science and Engineering
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• v.32 no.4
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• pp.35-41
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• 2018

The purpose of study intends to investigate effects of repetitive work and workload for body while wearing firefighting protective clothing in hot conditions and to provide the basic data for the safety of firefighter. The experimental temperature is WBGT $30^{\circ}C$ and the work intensity is 3, 9 METs. The experiment is processed 15 minutes (Ex1), 15 minutes rest (Rest), 15 minutes (Ex2) in order of time. A comparative experiment was carried out for firefighting school students(9 persons; $24.3{\pm}0.71$ years old) wearing firefighting protective clothing. When workload is increased from 3 METs to 9 METs, mean skin temperature change (33.3%), tympanic temperature change (242.9%), heart rate (36.2%), respiration rate (53.9%), RPE (81.6%), thermal Sensation (20.8%), weight loss (60.0%), glucose (-4.4%), lactate (41.7%) are statistically significantly higher. As a result, it is considered that repetitive work and workload have a strong effect on the body when wearing firefighting protective clothing in hot conditions.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.32 no.2
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• pp.182-194
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• 2022

Objectives: This study evaluates and recommends the priority of policy implementation to improve the fire protection clothing management system used by firefighters and the reduction of cross-contamination from contaminated clothing at the scene of a fire. Methods: It consisted of 7 experts and conducted three interviews and two modified Delphi surveys. Through the results of previous research and interviews with experts, a plan to reduce cross-contamination of fire suits and improve the management system was first derived. An improvement plan was presented in the four areas including resources, management, fire protection related work, and laws and regulations, and the priority of policy implementation was derived by analyzing the importance and practicality of the policy at the same time. Results: As a result of the analysis, the first priority was education on the health effects of pollutants at the disaster scene for firefighters, and the second priority was the addition of SOP for the primary decontamination of on-scene personal protective equipment in preparation for the health effects of the disaster scene, and education for fire suppression and rescue workers. The next step was to improve the management system of personal protective equipment such as fire suits and develop a training course for systematic operation. Conclusions: This findings could be used in the implementation of mid- to long-term firefighting policies for the systematic operation and establishment of a systematic management system for personal protective equipment such as fire protective suits.

• Journal of Families and Better Life
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• v.23 no.1 s.73
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• pp.19-29
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• 2005

The purpose of this study was to investigate ego-resilience and social adaptability of the children and adolescents in institutional care. The study also investigated the protective factors and the risk factors on ego-resilience and social adaptability and their relationship. The subjects in this study were 140 children and adolescents in institutional care. The research data were analyzed using Frequency, Pearson's Correlation, Stepwise Multiple Regression Analysis and T-Test in SPSS WIN 10.0 program. The major findings were as follows. First, ego-resilience of the children and adolescents in institutional care was related to locus of control, depression and anxiety and unsatisfaction of institutional care. Result of stepwise multiple regression analysis showed that depression and anxiety and locus of control in the order had significant effects on ego-resilience. Second, social adaptability of the children and adolescents in institutional care was related to social support and parent-child bond. Unsatisfaction of institutional care and social support in the order had significant effects on social adaptability by stepwise multiple regression analysis. Third, the children and adolescents who had higher level of ego-resilience reported better social adaptability. The overall research results revealed that the risk factors such as unsatisfaction of institutional care and depression and anxiety than the protective factors had more significant effects on ego-resilience and social adaptability of children and adolescents in institutional care. This finding suggested that elimination of risk factors rather than development of protective factors was a more important task for the welfare of children and adolescents in institutional care.

• The Journal of Korean Medicine
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• v.22 no.3
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• pp.1-10
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• 2001

Objectives : The water extract of Nueihyuljunbang (NHJB) has long been used for treatment of ischemic brain damage in Oriental Medicine. However, little is known about the mechanism by which the water extract of NHJB recovers brain cens from ischemic damage. Methods : To elucidate the protective mechanism on ischemic induced cytotoxicity, we investigated the regulation of lipopolysaccharide (LPS) and phorbol-12-myristate-13-acetate (PMA)-induced inducible nitric oxide synthase (iNOS) expression in C6 glial cells. Results : LPS combined PMA treatment for 72 hours in C6 glial cells markedly induced nitric oxide (NO), but treatment of the cells with the water extract of NHJB decreased dose-dependently nitrite formation. In addition, LPS combined PMA treatment for 72 hours induced severe celt death and lactate dehydrogenase (LDH) release in C6 glial cells. However, treatment of the celts with the water extract of NHJB did not induce significant change compared to control cells. Furthermore, the protective effects of the water extract of NHJB were mimicked by the treatment of NGMMA, a specific inhibitor of NOS. LPS combined PMA induced iNOS activation in C6 glial cells caused chromosomal condensation and fragmentation of the nuclei by caspase activation. The treatment of C6 glial cells with the water extract of NHJB might suppress apoptosis via caspase inhibition by regulation of iNOS expression. Conclusions : From the results, we suggest that the protective effects of the water extract of NHJB against ischemic brain damage may be mediated by regulation of iNOS during ischemic condition.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.2
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• pp.297-307
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• 2003

In the present study, we investigated the protective effect of the Puerarie Radix water extract (PRE) against CCl₄-induced hepatotoxicity and the mechanism underlying these protective effects in the rats. The pretreatment of PRE has shown to possess a significant protective effect by lowering the serum alanine and aspartate aminoteansferase (AST and ALT) and alkaline phosphatase (ALP). This hepatoprotective action was confirmed by histological observation. In addition, the pretreatment of PRE prevented the elevation of hepatic malondialdehyde (MDA) formation and the depletion of reduced glutathione (GSH) content and catalase activity in the liver of CC1₄-injected rats. The PRE also displayed hydroxide radical scavenging activity in a dose-dependent manner (IC50 = 83.6 μg/ml), as assayed by electron spin resonance (ESR) spin-trapping technique. Moreover, the expression of cytochrome P450 2E1 (CYP2E1) mRNA, as measured by reverse transcriptase-polymerase chain reaction (RT-PCR), was significantly decreased in the liver of PRE-pretreated rats when compared with that in the liver of control group. Based on these results, it was suggested that the hepatoprotective effects of the PRE may be related to antioxidant effects and regulation of CYP2E1 gene expression.

• Biomolecules & Therapeutics
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• v.25 no.6
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• pp.599-608
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• 2017

Tanshinone IIA (Tan IIA) is a pharmacologically active substance extracted from the rhizome of Salvia miltiorrhiza Bunge (also known as the Chinese herb Danshen), and is widely used to treat atherosclerosis. The pregnane X receptor (PXR) is a nuclear receptor that is a key regulator of xenobiotic and endobiotic detoxification. Tan IIA is an efficacious PXR agonist that has a potential protective effect on endothelial injuries induced by xenobiotics and endobiotics via PXR activation. Previously numerous studies have demonstrated the possible effects of Tan IIA on human umbilical vein endothelial cells, but the further mechanism for its exerts the protective effect is not well established. To study the protective effects of Tan IIA against hydrogen peroxide ($H_2O_2$) in human umbilical vein endothelial cells (HUVECs), we pretreated cells with or without different concentrations of Tan IIA for 24 h, then exposed the cells to $400{\mu}M$ $H_2O_2$ for another 3 h. Therefore, our data strongly suggests that Tan IIA may lead to increased regeneration of glutathione (GSH) from the glutathione disulfide (GSSG) produced during the GSH peroxidase-catalyzed decomposition of $H_2O_2$ in HUVECs, and the PXR plays a significant role in this process. Tan IIA may also exert protective effects against $H_2O_2$-induced apoptosis through the mitochondrial apoptosis pathway associated with the participation of PXR. Tan IIA protected HUVECs from inflammatory mediators triggered by $H_2O_2$ via PXR activation. In conclusion, Tan IIA protected HUVECs against $H_2O_2$-induced cell injury through PXR-dependent mechanisms.

• The Journal of Internal Korean Medicine
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• v.43 no.3
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• pp.344-362
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• 2022

Objective: In this study, the antioxidant activity of Ojayeonjong-hwan extracts was compared, and the following results were obtained. Methods: For hydrothermal and ethanol extracts, DPPH free radical and ABTS cationic radical erasing activity and reducing power using the FRAP method were compared, and the association between the antioxidant power of each extract and total phenol content was investigated. Significant results were obtained through in vitro apoptosis analysis through FFITC staining, mitochondrial membrane potential analysis, and ROS level measurement using C2C12 myoblastoma. Results: 1. In a comparison of DPPH free radical and ABTS cationic radical scavenging activity, water, and 70% ethanol extracts of Ojayeonjong-hwan (WEO and EEO) showed superior radical scavenging ability. 2. In the results of reducing power using the FRAP method, WEO and EEO showed antioxidant activity, which was shown to be dependent on the total phenol content contained in the extracts. 3. In comparison to the protective effect against H₂O₂-induced oxidative stress in C2C12 myoblasts, water extracts had no significant effect, but 70% ethanol extracts inhibited H₂O₂-mediated cytotoxicity in a concentration-dependent manner. 4. The cytotoxic protective effect of EEO against oxidative stress in C2C12 myoblasts was correlated with its inhibitory effects on H₂O₂-induced apoptosis and cell-cycle arrest. 5. In H₂O₂-treated C2C12 myoblasts, the apoptosis inhibitory effects of EEO were associated with the suppression of mitochondrial dysfunction and DNA damage. 6. The protective effects of EEO against H₂O₂-induced oxidative stress in C2C12 myoblasts were directly related to the inhibition of ROS generation. Conclusions: Ojayeonjong-hwan extracts all have protective potential against oxidative stress.

• Korean Journal of Culture and Social Issue
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• v.14 no.1_spc
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• pp.273-298
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• 2008

The purpose of this study is to explore a risk and a protective factors of adolescent runaway based on the previous researches. Runaway of adolescents is increasing, and it is longer and more habit gradually than past. The prevailance methods of runaway is no longer effects on the decrease of adolescent runaway. Now there is to accept adolescent runaway, and has to support the difficult life conditions and the emotional distress of adolescent post-runaway. This means that must be identify and treatment the protective factors having an buffering effect as well as the risk factors influencing on runaway. Although the worse factors related family are the highest risk factors of runaway, others factors including negative peer relation, a academic stress also an have significant influence on runaway. And the protective factors mediating the negative influence of risk factors are an monitoring and controlling of parent as authoritative rearing attitude, a social support, a positive coping strategies, a self-esteem, and a conventional peer group. Protective factors prevent from runaway behavior, as well as improve a resilience of adolescents in the poor environment. The tasks of follow study are to investigate empirically the mediating effects of the protective factors on the problem behavior of post-runaway and resilience of runaway adolescents based on the systematic research design.

• International Journal of Oral Biology
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• v.33 no.4
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• pp.137-141
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• 2008

The present study reports the protective properties of a total methanol extract of B. platyphylla var. japonica against ultraviolet (UV)-C irradiation. Pretreatment of Chinese hamster fibroblast (V79-4) cells with a total methanol extract significantly increased cell survival following $300\;J/m^2$ of UV-C irradiation. The total methanol extract was further fractionated into 5 fractions: n-hexane, dichloromethane, ethylacetate, n-butanol and water fractions. Among these fractions, B. platyphylla var. japonica ethylacetate, butanol and water fractions showed significant protective effects against the cellular damage induced by UV-C irradiation. In order to elucidate the mechanism underlying this protective effect, DPPH (Editor note: abbreviations should be spelled out at first use.) radical scavenging and lipid peroxidation inhibitory activity were measured. Significant radical scavenging and lipid peroxidation inhibitory activities were observed for the ethylacetate fraction. In summary, the present data demonstrate that an extract of B. platyphylla var. japonica has a significant protective effect against UV-C irradiation. The underlying mechanism of this protective effect may involve radical scavenging and inhibition of lipid peroxidation by the B. platyphylla var. japonica extract.