대한한의학회지 (The Journal of Korean Medicine)

  • 제22권3호
  • /
  • Pages.1-10
  • /
  • 2001
  • /
  • 1010-0695(pISSN)
  • /
  • 2288-3339(eISSN)
대한한의학회 (The Society of Korean Medicine)
권호 표지

LPS와 PMA에 손상된 신경교세포에 대한 뇌혈전방의 방어효과

Protective Effects of Nueihyuljunbang on LPS Combined PMA Induced Cytotocity in C6 Gilal Cell

  • 서관수 (원광대학교 한의과대학 심계내과학교실) ;
  • 문병순 (원광대학교 한의과대학 심계내과학교실) ;
  • 성강경 (원광대학교 한의과대학 심계내과학교실) ;
  • 임규상 (원광대학교 한의과대학 외관과학교실) ;
  • 신선호 (원광대학교 한의과대학 심계내과학교실)
  • 발행 : 2001.09.01
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초록

Objectives : The water extract of Nueihyuljunbang (NHJB) has long been used for treatment of ischemic brain damage in Oriental Medicine. However, little is known about the mechanism by which the water extract of NHJB recovers brain cens from ischemic damage. Methods : To elucidate the protective mechanism on ischemic induced cytotoxicity, we investigated the regulation of lipopolysaccharide (LPS) and phorbol-12-myristate-13-acetate (PMA)-induced inducible nitric oxide synthase (iNOS) expression in C6 glial cells. Results : LPS combined PMA treatment for 72 hours in C6 glial cells markedly induced nitric oxide (NO), but treatment of the cells with the water extract of NHJB decreased dose-dependently nitrite formation. In addition, LPS combined PMA treatment for 72 hours induced severe celt death and lactate dehydrogenase (LDH) release in C6 glial cells. However, treatment of the celts with the water extract of NHJB did not induce significant change compared to control cells. Furthermore, the protective effects of the water extract of NHJB were mimicked by the treatment of NGMMA, a specific inhibitor of NOS. LPS combined PMA induced iNOS activation in C6 glial cells caused chromosomal condensation and fragmentation of the nuclei by caspase activation. The treatment of C6 glial cells with the water extract of NHJB might suppress apoptosis via caspase inhibition by regulation of iNOS expression. Conclusions : From the results, we suggest that the protective effects of the water extract of NHJB against ischemic brain damage may be mediated by regulation of iNOS during ischemic condition.

키워드

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