This study was carried out to investigate the optimal culture conditions for gametophytes growth and sporophytes regeneration of Pyrrosia linearifolia in order to provide for the masspropagation system foundation of Pyrrosia linearifolia using their life cycle. Among many different media, 2MS medium was most effective in prothallus proliferation. Prothallus growth was promoted as the total concentration of nitrogen sources increased, and the best result was observed on 120 mM nitrogen. The best concentration of sucrose was 3%. The addition of 5~20 mM IAA, NAA, BA and kinetin promoted the propagation of prothallus. But 2iP demonstrated the most inhibitory effect on prothallus proliferation. Gametophytes shaking-cultured with liquid medium showed similar growth with solid medium and normal formation of reproductive organs. Shoot regeneration was most effective on 1/8MS medium, but growth was promoted on 1/2MS medium. For promotion of shoot regeneration and growth, the suitable concentrations of sucrose and $NaH_2PO_4$ were 1% and $50{\sim}100mg{\cdot}mL^{-1}$ in 1/8MS medium, respectively.
Kim, Seung-Tae;Kim, Won-Hee;Lee, Eun-Kyung;Lee, Su-Young;Mun, Bo-Heum;Lee, Kwang-Sik
FLOWER RESEARCH JOURNAL
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v.19
no.3
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pp.164-167
/
2011
A new spray rose cultivar 'Glory Purple' was bred from the cross between a pink spray cultivar 'Macarena' and a pink spray cultivar 'Million Pink' at the National Institute of Horticulture & Herbal Science. The cross was made in 2004 and a candidate genotype which named as 'Glory Purple' was selected in 2009 after three years investigation for agronomical characters from 2007 to 2009. 'Glory Purple', a purple colored spray cultivar, has vigorous growth and has powdery mildew resistance. The major characteristics of this cultivar are high yield and long stem with relatively large flower size with $157stems/m^2/year$ in yield, 63.5 cm in mean length of cut flower, 6.8 cm in mean flower diameter, 25.78 in mean petal number, and 10.2 days in mean vase life. This cultivar is suitable for propagation by both cutting and grafting methods. The consumer's preference of this cultivar is relatively higher than that of the control cultivars, 'Pinky and Charming'.
The ovipositional characteristics of the endangered species Prosopocoilus astacoides blanchardi was investigated for indoor mass-rearing. This insect required 3 weeks or more for mating after emergence when introduced at the 1:1 ratio of male to female. Among temperatures studied at 16, 20, 24, 28, and 32℃, the pre-ovipositional period of P. astacoides blanchardi was longest at 16℃ with an average of 109 days after emergence, while it was shortest at 32℃ with an average of 59.4 days. The number of eggs laid at 24℃ was highest with an average of 40.6 per female. The average lifespan of adults at 16℃ was 208.8 days for males and 263.9 days for females, while at 32℃ 96.1 days for males and 130.3 days for females. The number of laid eggs was dependent on insect rearing chamber size. The oviposition-trees did not significantly (P > 0.05) affect the number of laid eggs and ovipositional period. The results of this study could be used as rearing data for the restoration and mass propagation of the endangered species P. astacoides blanchardi.
Maulana, Sena;Gumelar, Yuarsa;Fatrawana, Adesna;Maulana, Muhammad Iqbal;Hidayat, Wahyu;Sumardi, Ihak;Wistara, Nyoman Jaya;Lee, Seung Hwan;Kim, Nam Hun;Febrianto, Fauzi
Journal of the Korean Wood Science and Technology
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v.47
no.4
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pp.519-532
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2019
The objectives of this study were to evaluate the effects of shelling ratio and resin content on the properties of bamboo oriented strand board (BOSB) from betung (Dendrocalamus asper) and to determine the correlation between the results of dynamic and static bending tests. Strands were steam-treated at $126^{\circ}C$ for 1 h under 0.14 MPa pressure and followed by washing with 1% NaOH solution. Three-layer BOSB with the core layer perpendicular to the surface was formed with shelling ratios (face:core ratio) of 30:70; 40:60; 50:50; 60:40 and binded with 7% and 8% of phenol formaldehyde (PF) resin with the addition of 1% of wax. The evaluation of physical and mechanical properties of BOSB was conducted in accordance with the JIS A 5908:2003 standard and the results were compared with CSA 0437.0 standard for commercial OSB (Grade O-1). Non-destructive testing was conducted using Metriguard Model 239A Stress Wave Timer which has a wave propagation time from 1 to $9,999{\mu}s$ and a resolution of $1{\mu}s$. BOSB with 8% resin content showed better physical and mechanical properties than those with 7% resin content. The increase of the face layer ratio improved the strength of BOSB in parallel direction to the grain. The results suggested that shelling ratio of 50:50 could be used as a simple way to reduce PF resin requirements from 8% to 7% and to meet the requirements of CSA 0437.0 standard. The results of non-destructive and destructive tests showed a strong correlation, suggesting that non-destructive test can be used to estimate the bending properties of BOSB.
The Journal of the Convergence on Culture Technology
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v.7
no.4
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pp.721-726
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2021
Plant cells have a totipotencial capacity, the ability of each cell to produce a new complete individual through development. By applying this, several technologies are being developed for widespread application of somatic embryogenesis by processing hormones in vitro as a method of propagation of plants. In order to use this technology, in Kalanchoe pinnata, a plant capable of asexual reproduction with more regular cell division, kinetin belonging to cytokinin and picloram among hormones belonging to auxin were added in combination and treated for 8 weeks, and then the typical performance was evaluated. As a result of our experiment, the rooting effect in leaf slices showed a 70% incidence rate at a picloram concentration of 0.1 mg/L. It has been proven that a concentration difference of 1:5-1:10 in the ratio of kinetin and picloram is effective. It is the experimental result that the effect of auxin is essential for the development of Kalanchoe roots. As for the effect of shooting, the incidence rate was 60% at the picloram concentration of 0.5 mg/L. The kinetin concentration from 0.5 and 1.0 mg/L and has a significant effect on development. It has been proven that the ratio of kinetin to picloram is effective with a concentration difference of 1:1-1:2. These results show that the combination of cytokinin and auxin is crucially important for shooting. It is thought that it can be the basis of a technology for inducing mass proliferation in vitro by inducing direct organogenesis with a combination of hormones.
Kang, Hee-Nam;Jeong, Dong Il;Kim, Young Il;Kim, In Yeong;Park, Sang Cheol;Nam, Cheol Woo;Seo, Seok-Jun;Lee, Jin Yeong;Lee, Bin
Journal of Powder Materials
/
v.29
no.1
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pp.47-55
/
2022
Tungsten carbide is widely used in carbide tools. However, its production process generates a significant number of end-of-life products and by-products. Therefore, it is necessary to develop efficient recycling methods and investigate the remanufacturing of tungsten carbide using recycled materials. Herein, we have recovered 99.9% of the tungsten in cemented carbide hard scrap as tungsten oxide via an alkali leaching process. Subsequently, using the recovered tungsten oxide as a starting material, tungsten carbide has been produced by employing a self-propagating high-temperature synthesis (SHS) method. SHS is advantageous as it reduces the reaction time and is energy-efficient. Tungsten carbide with a carbon content of 6.18 wt % and a particle size of 116 nm has been successfully synthesized by optimizing the SHS process parameters, pulverization, and mixing. In this study, a series of processes for the high-efficiency recycling and quality improvement of tungsten-based materials have been developed.
This study aims to understand human nature in Daesoon Thought. In addition, it seeks to determine the characteristics of human nature and how the theory of human nature in Daesoon Thought relates to the direction of the Basic Works and the Three Major Societal Works of Daesoon Jinrihoe. In order to understand the theory of human nature in Daesoon Thought, I have classified various theories of human nature that appear in the Confucian tradition and have analyzed and reviewed them. I used this framework of types as a methodological criterion to study the theory of human nature found in Daesoon Thought. In Daesoon Thought, human nature is defined clearly as the conscience that is honest and truthful. Also, it accepts humans' basic biological needs, the pursuit of clothing, food, and sexual desires as part of human nature. This view can be evaluated as quite similar, although not perfectly consistent, with the theory of human nature posited by Wang Fuzhi (王夫之), who defined human nature as a combination of morality and biological desires. The theory of human nature in Daesoon Thought emphasizes human morality, but at the same time, it fully accepts that biological needs are basic elements of human life. In addition, it shows a characteristic that strongly implies a religious tendency as it recognizes that human nature is internalized through divine commands issued by the Supreme God of the Ninth Heaven (九天上帝 Gucheon Sangje), the transcendent and absolute ruler. The Basic Works of Daesoon Jinrihoe, which are Propagation, Edification, and Cultivation, aim to restore human nature to its innate state of honesty and truthfulness. Among the Three Major Societal Works, we can see that the works of Social Welfare and Charity Aid are implemented in accordance with the accepting acknowledgment of basic human needs (the pursuit of clothing, food, and sexual desires), and helping people meet their needs in appropriate ways and to appropriate degrees.
Edification in Daesoonjinrihoe is not only a phenomenon that occurs following the differences of religious experience or spiritual development among the community members, which enables the members to share teaching and learning experiences with one another, but also an issue determined as one of the major activities of the religious order and a plan for achieving the purpose of the religious order-Podeokchenha(Wordly Propagation), Gujechansaeng (Salvation of all mankind) and Jisangcheonguk Geonseol(Building of earthly paradise). The purpose of this article is to clarify its concept and provide an example of edification, through considering the historical model for edification to help the cultivators with their work of edification. The archetype of edification of Daesoonjinrihoe was formed and gradually developed in phases by Sangje, Kang Jeungsan, the Supreme God(姜甑山, 1871-1909), Doju, Jo Jeongsan(趙鼎山, 1895-1958) and Dojeon, Park Wudang(朴牛堂, 1917-1995), by the three of whom the Religious Authority was succeeded. Sangje descended to the human world and preached to people to live by the rule of Haewon Sangsaeng(Resolution of grievances for the mutual beneficences of all life) and set an example of abolishing the old customs, living in mutual beneficences and having respect for human being. Doju, in revering the last will of Sangje, established the religious order by setting its creed, rituals and activities, which formed most contents of the archetype of edification. Dojeon set up a religious faith system by firmly establishing the Religious Authority and performed the True Law in accordance with Sangje's program of heaven to educate the cultivators to achieve the goal of self-cultivation following the last will of Doju. Through this, a perfect method to reach the state of Dotong(The Truly Unified State of Dao) is fulfilled. In this way, the archetype of edification was formed in the process of succession of Religious Authority. In conclusion, edification in Daesoonjinrihoe contributes to a 'systematic conveyance and understanding' through the historical archetype of edification, and it can be described as a concept that becomes a model to put into practice the 'True Law' of teachings given by two Sangjes for Dotong. Therefore, edification of Daesoonjinrihoe is drawing attention of its development as an important activity that realizes the ultimate value of the religious order because it solves the problems of immorality(absence of Dao), disorder and disregard of human value generated from the other side of this material civilization, with the truth of Haewon Sangsaeng, and has a function of rebuilding and leading the individuals and the society to the Truly Unified State of Dao through performing of the True Law.
Ji, Bo-Young;Kim, Ki-Hong;Park, Soo-Il;Kim, Yi-Cheong
Journal of fish pathology
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v.11
no.1
/
pp.51-60
/
1998
Concerned to the lyfe cycle of Ichthyophthirius multifiliis, the experimental infection and development of the parasites were studied in the several freshwater cultured fishes. Opitimum conditions for the propagation of the parasite by serial passage with the rainbow trout fry was observed. Visiable white spots were examined in the body surface, fins and gills of the healthy fries, and a stable infection has been maintained for 2 months in the experimental system (Temperature: $18{\pm}1^{\circ}C$ DO: 7-7.5 ppm; pH: $7{\pm}0.2$). Induction conditions for artificial infection of the parasite by interms of the host fishes, stages of the parasites, and rearing temperature regimes was investigated. Rainbow trout fries showed a positive infection which was resulted from exposure of theront at $18^{\circ}C$. The rainbow trout fries induced white spots on the body surface at 3-7 days exposure to the theronts at $18^{\circ}C$. It was found that exposure of the rainbow trout fries exposed to 1,000 theronts per fish (10 theront/ml) for 45-60 minutes at $18^{\circ}C$ would consistently produce infection. Perfect infection (100%) was induced when the fries were exposed to 1,500 theront per fish (15 theront/ml) under laboratory condition. Development of I. multifiliis in the rainbow trout was observed for 7 days postexposure (PE). The parasite increased in average diameter from $54{\mu}m$ on the 1st day PE to $426{\mu}m$ on the 7th day PE. In the initial infestation period, the parasites were found on the gill epithelium, and on the 3rd day PE they invaded into the basal part of the gill filament adjacent to the major blood vessels, particularly the afferent vessels. Morphological change of buccal apparatus were observed on the 2nd day PE. Contractile vacuoles were more prominent on the 4th day PE, and they had notable changes on the 7th day PE.
A study was conducted to investigate the effect of topophysis, and uniting method of rootstock and scion on rooting and subsequent growth of stenting-propagated cut rose ($Rosa$$hybrida$ Hort.) in an effort to develop an efficient stenting propagation method for domestic rose cultivars. Four cultivars used in this study were two standard type cultivars 'Sweet Yellow' and 'Hanmaum', and two spray type cultivars 'Chelsi' and 'May'. Scions were grafted on cuttings of a rootstock $Rosa$$indica$ 'Major'. The stenting-propagated scion-rootstock unions were planted in rockwool cubes ($50{\times}50{\times}50mm$, Delta, Grodan, Denmark) and were placed in a graft-take chamber for five days before being placed on misted greenhouse beds. The rootstock was removed of all leaves and nodes. Both the base of scions and top of stocks were simultaneously cut at a $45^{\circ}$ angle for grafting. Scions were prepared as single node cuttings, each with a five-leaflet leaf. Three positions of topophysis used were 7-9th (top), 4-6th (middle), and 1st-3rd (bottom) nodes from the stem base. Four uniting materials used were tube, tube + parafilm wrap, tube + clothespin, and clothespin. Rooting and growth were affected by the topophysis and cultivar. The best topophysis for rooting was 7-9th (top) nodes in all cultivars. Topophysis affected percent rooting, and number of roots, length of the longest root, and but not weight, shoot length and graft-take. Rooting and growth were affected by the uniting method and cultivar. Tube uniting method generally showed higher percentage graft-take, percent rooting, and number of roots than other methods. However, rootstock and scion union was not complete in this treatment. On the whole, the greatest rooting and subsequent growth of stenting-propagated plants were found in the tube + clothespin method. Except 'Sweet Yellow', rooting and growth were not adequate in the clothespin method. The results suggested that a tube + clothespin method was the most effective, and this method may be used as a substitute to save labor compared to a tube + parafilm wrap method which is currently being used in commercial nurseries.
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