• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.199-202
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• 2000

Optimization of submerged culture conditions for the production of exo-biopolymer from Paecilomyces japonica was studied. Maltose, yeast extract and potassium phosphate were the most suitable sources of carbon, nitrogen, and inorganic salt, respectively, for both production of the exo-biopolymer and mycelial growth. The optimal culture conditions in flask culture were pH 5.0, $25^{\circ}C$ and 150 rpm in a meidum containing of 30 g maltose, 6 g yeast extract, 2 g polypeptone, 0.5 g $K_2HPO_4$, 0.2 g $KH_2PO_4$, 0.2 g $MnSo_4\;{\cdot}\;5H_2O$, 0.2 g $MgSO_4\;{\cdot}\;7H_2O$ in 1-L distilled water. Exo-biopolymer production and mycelial growth in the suggested medium were significantly increased in a 2.5-L jar fermentor, where the maximum biopolymer concentration was 8 g/1.

• KSBB Journal
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• v.14 no.2
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• pp.255-258
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• 1999

Effects of growth conditions on the growth of Zoogloea ramigera and the production of zooglan were investigated. The production of zooglan was greatly reduced in the phosphate-limiting medium. $NH_4Cl$ and ${(NH_4)}_2SO_4$ improved cell growth when they were used as a nitrogen source. The medium containing 45 g/L of glucose and 27 g/L of $NaNO_3$ resulted in the highest production of zooglan at 18.5 g/L.

• Journal of Microbiology and Biotechnology
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• v.17 no.6
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• pp.1049-1053
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• 2007

Tannase production by Aureobasidium pullulans DBS66 was optimized. The organism produced maximum tannase in the presence of 1% tannic acid after 36 h. Maximum gallic acid accumulation was observed within 36 h and tannic acid in the fermented broth was completely degraded after 42 h of growth. Glucose had a stimulatory effect on tannase synthesis at 0.1% (w/v) concentration. The organism showed maximum tannase production with $(NH_4)_2HPO_4$ as nitrogen source. Shaking speed of 120 rpm and 50-ml broth volume have been found to be suitable for maximum tannase production.

• Journal of Hydrogen and New Energy
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• v.24 no.4
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• pp.275-281
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• 2013

This study was conducted to increase the amount of bio-hydrogen production from microalgae(Chlorella vulgaris) in batch reactors by thermal pre-treatment. The optimization of thermal pre-treatment was conducted using statistic experimental design of response surface methodology. Two experimental parameters of temperature and reaction time were considered. The optimization condition was founded at the coded variables of <0.52, -0.07> corresponding to the experimental of heating temperature of $95.6^{\circ}C$ and reaction time of 57.9 min, respectively. Under the optimal condition, the maximum hydrogen production was predicted to 25.3mL $H_2/g$ dry cell weight (dcw), which was 9.1 times higher value of control(2.8mL $H_2/g$ dcw).

• Food Science and Biotechnology
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• v.18 no.2
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• pp.442-448
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• 2009

In this study Bacillus subtilis PTCC 1023 was used for the production of protopectinase using soybean based media. The use of isolated soybean protein (ISP) and soybean flour resulted in similar protopectinase production and growth rates. The effect of medium composition on protopectinase production was studied using central composite design (CCD) methodology. The change in the concentration of ISP (1-7%), glucose (0-10%), and phosphate (0.1-0.3 M) was found to affect the protopectinase activity (response variable) after 24 hr of cultivation. In the range studied, ISP and glucose had a negative effect on the response variable, whereas phosphate had a positive effect. A statistically significant interaction was identified between phosphate and ISP, suggesting that correct optimization of medium formulation in this case can only be obtained using factorial design of experiments. Protopectinase activity exceeding 215 U/mL was obtained in a medium containing 4% ISP, 0.3M phosphate, and no added sugar.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2004.10a
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• pp.527-530
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• 2004

This paper presents an optimization formulation for resource scheduling in Critical Resource Diagramming (CRD) of production scheduling networks. A CRD network schedules units of resources against points of needs in a production network rather than the conventional approach of scheduling tasks against resource availability. This resource scheduling approach provides more effective tracking of utilization of production resources as they are assigned or 'moved' from one point of need to another. Using CRD, criticality indices can be developed for resource types in a way similar to the criticality of activities in Critical Path Method (CPM). In our supply chain model, upstreams may choose either normal operation or expedited operation in resource scheduling. Their decisions affect downstream's resource scheduling. The suggested optimization formulation models resources as CRD elements in a production two-stage supply to minimize the total operation cost

• Korean Journal of Computational Design and Engineering
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• v.19 no.2
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• pp.148-156
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• 2014

Generally, over 95% of manufacturing cost is determined in the design and manufacturing preparation step, especially a great part of productivity is determined in the manufacturing preparation step. In order to improve the manufacturing competitiveness, we have to verify the problems that can be occurred in the production step and remove the unnecessary factors in the manufacturing preparation step. Thus, manufacturing industries are adopting digital manufacturing system based on modeling & simulation. In this paper, we introduce e-FEED system (electronic based Front End Engineering and Design) that is the integrated design and analysis system for optimized manufacturing line development based on simulation automation and explain the work process (Design, Analysis and Optimization) about manufacturing line development using e-FEED system. Also, the effect is described through the real implementation cases.

• Journal of Ocean Engineering and Technology
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• v.24 no.1
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• pp.20-33
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• 2010

The paper deals with strength design of a riser support installed on floating production storage and offloading (FPSO) vessel under various loading conditions - operation, extreme, damaged, one line failure case (OLFC) and installation. The design problem is formulated such that thickness sizing variables are determined by minimizing the weight of a riser support structure subject to stresses constraints. The initial design model is generated based on an actual FPSO riser support specification. The finite element analysis (FEA) is conducted using MSC/NASTRAN, and optimal solutions are obtained via moving least squares method (MLSM) in the context of response surface based approximate optimization. For the meta-modeling of inequality constraint functions of stresses, a constraint-feasible moving least squares method (CF-MLSM) is used in the present study. The method of CF-MLSM, compared to a conventional MLSM, has been shown to ensure the constraint feasibility in a case where the approximate optimization process is employed. The optimization results present improved design performances under various riser operating conditions.

• Microbiology and Biotechnology Letters
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• v.25 no.2
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• pp.212-217
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• 1997

A strain of Bacillus sp. WS-14 was isolated from soil. Medium optimization for ${\beta}-mannanase$ production by Bacillus sp. WS-14 was performed. Effect of various carbon sources on ${\beta}-mannanase$ production was investigated and locust bean gum was the most effective for ${\beta}-mannanase$ production. ${\beta}-mannanase$ activity and cell growth increased with increasing the concentration of locust bean gum, however, the amounts were not significant. Among nitrogen sources, soytone was the most effective for ${\beta}-mannanase$ production. Inorganic compounds such as $KH_2PO_4,\;NaCl\;Na_2CO_3\;and\;MgSO_4{\cdot}7H_2O\;on\;{\beta}-mannanase$ production were optimized for ${\beta}-mannanase$ production. Locust bean gum of 10.0 g/l, soytone of 5.0 g/l, $KH_2PO_4$ of 2.0 g/l, NaCl of 10.0 g/l, $MgSO_4{\cdot}7H_2O\;of\;0.2\;g/l,\;Na_2CO_3$, of 2.0 g/l were selected as optimum content. Production of ${\beta}-mannanase$ by using the optimum medium was carried out. The maximum ${\beta}-mannanase$ activity of 20.8 unit/ml could be obtained after 14 h fermentation which corresponed to the productivity of ${\beta}-mannanase$ of 1.48 unit/ml-h.

• Journal of Microbiology and Biotechnology
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• v.18 no.1
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• pp.138-144
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• 2008

The production of the entomopathogenic and phytotoxic cyclic depsipeptide beauvericin (BEA) was studied in submerged cultures of Fusarium oxysporum KFCC 11363P isolated in Korea. The influences of various factors on mycelia growth and BEA production were examined in both complete and chemically defined culture media. The mycelia growth and BEA production were highest in Fusarium defined medium. The optimal carbon and nitrogen sources for maximizing BEA production were glucose and $NaNO_3$, respectively. The carbon/nitrogen ratio for maximal production of BEA was investigated using response surface methodology (RSM). Equations derived by differentiation of the RSM model revealed that the production of BEA was maximal when using 108 mM glucose and 25 mM $NaNO_3$.