• 한국산학기술학회논문지
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• 제20권6호
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• pp.587-591
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• 2019

미세먼지는 대기를 타고 호흡기를 거쳐 폐 혹은 혈관에 침투 한다. 최근 미세먼지 문제 때문에 국내에 공기청정기 수요도 급증하고 있다. 이끼는 가장 오래된 지상식물로 중금속과 미세먼지를 흡착하여 분해하는 성질을 가지고 있는 것으로 알려졌고 본 연구진은 이를 활용하여 화학적 필터를 대체 할 수 있는 시스템에 대해 연구하였다. 이끼와 기존 화학적 필터(Hepa)의 미세먼지 감소 효과를 비교하기 위하여 $1m^3$의 큐브를 제작하고 통제된 환경하에서 미세먼지의 감소량을 비교하였다. 미세먼지 상황 하에서 이끼, 헤파, 무필터를 탑재한 공기정화 시스템을 각 30회씩 총 90회 가동 하였고 각각의 미세먼지 감소량 및 감소율을 비교한 결과 감소량은 미세먼지 이끼필터를 사용한 평균이 138.93으로 헤파필터를 사용한 평균 76.57과 무필터 상태의 평균인 0.10보다 유의하게 높았으며 감소율의 평균 역시 이끼필터를 사용한 경우 0.2379로 헤파필터를 사용한 경우의 0.1298이나 무필터인 0.0063보다 유의하게 높았다. 이 결과를 통해 이끼의 미세먼지 감소효과를 확인하였고 향후 개선을 통해 기존 공기정화기의 화학필터를 대체하거나 보조할 것으로 기대된다.

• Journal of Microbiology and Biotechnology
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• 제21권5호
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• pp.470-476
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• 2011

A potent fungus for amylase production, Chrysosporium asperatum, was isolated from among 30 different cultures obtained from wood samples collected in the Junagadh forest, India. All of the isolated cultures were screened for their ability to produce amylase by submerged fermentation. Among the selected cultures, C. asperatum (Class Euascomycetes; Onygenales; Onygenaceae) gave maximum amylase production. In all of the different media tested, potato starch was found to be a good substrate for production of amylase enzyme at $30^{\circ}C$ and pH 5.0. Production of enzyme reached the maximum when a combination of starch and 2% xylose, and organic nitrogen (1% yeast extract) and ammonium sulfate were used as carbon and nitrogen sources, respectively. There was no significant effect of metal ions on enzyme activity. The enzyme was relatively stable at $30^{\circ}C$ for 20 min, and no inhibitory effect of $Ca^{+2}$ ions on amylase production was observed.

• 공업화학
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• 제31권1호
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• pp.1-6
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• 2020

바이오가스를 이용한 수소 제조는 주요한 에너지 및 환경 관련 이슈들을 동시에 해결할 수 있다는 장점으로 꾸준히 주목받아 왔다. 바이오가스 정제를 통해 얻은 바이오메탄 수증기개질은 천연가스 개질을 대체할 수 있는 좋은 현실적인 대안이다. 하지만, 경제성과 환경 유해성을 모두 고려한다면 바이오가스를 직접 개질반응에 활용하는 바이오가스 수증기 개질 및 건식 개질을 활용한 수소 제조가 보다 효과적이라 평가된다. 본 논문에서는 바이오가스 기반 추출수소 제조 관련 최근의 기술 이슈 및 개발 동향을 소개하며 향후 상업화를 위한 효과적인 적용 방향에 대해서 고찰하고자 한다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.616-616
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• 2003

• 한국유가공학회:학술대회논문집
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• 한국유가공기술과학회 1996년도 추계 제43회 유가공 심포지움
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• pp.30-34
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• 1996

Twenty seven strains of Lactobacillus acidophilus among 92 isolated from fecal contents of humans, pigs, calves, chickens, rodents and turkeys demonstrated inhibitory attributed to bacteriocin(s). The bacteriocin(s) were heat stable and nondialyzable proteinous compounds and exhibited narrow inhibitory spectra of activity. Neither hydrogen peroxide nor low pH were responsible for inhibitory action. All of the producer strains were resistant to their own bacteriocin or bacteriocin(s) produced by other strains. The bacteriocins from several strains from different host species were purified for further characterization. The bacteriocin(s) all exhibited similar characteristics.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.535-538
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• 2000

Human leptin is a 16 kDa (146 amino acids) protein secreted from adipocytes and influences body weight homeostasis. In this report, active human leptin was successfully produced in the culture medium of Bacillus subtilis. After simple purification steps consisting of ammonium sulfate precipitation and anion-exchange column chromatography, 2.3 mg of leptin with a purity of greater than 95% was obtained from the 0.5 L culture with the recovery yield of 54.9%. The purified leptin showed the correct folding structure with one disulfide bond.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.504-507
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• 2002

A separation/purification process for enzymatic sugar ester production was investigated The crude reaction mixture contained sugar ester and unreacted fatty acid in acetone. The reaction mixture was mixed with KOH/phosphate buffer. Hexane was then added to enhance phase separation. Three phases formed: a lower aqueous phase containing nothing of interest, a polar organic solvent middle phase that contained mostly fatty acid soaps, and a hexane-rich upper phase that contained mostly sugar ester. Distribution coefficient of each component was measured.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.316.2-317
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• 2003

Immunoglobulin Y (IgY) obtained from chicken as the immunization host brings several advantages to antibody production, such as improved yield, lower cost, longer stability, and higher specificity than mammalian immunoglobulin. In the present study, we attempted to purify Maackia fauriei lectin using antilectin IgY-affinity chromatography in order to produce a good yield and to reduce the purification time. (omitted)

• Parasites, Hosts and Diseases
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• 제44권1호
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• pp.35-41
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• 2006

Protozoan parasites of the genus Leishmania cause a number of important human diseases. One of the key determinants of parasite infectivity and survival is the surface glycoconjugate lipophosphoglycan (LPG). In addition, LPG is shown to be useful as a transmission blocking vaccine. Since culture supernatant of parasite promastigotes is a good source of LPG, we made attempts to characterize functions of the culture supernatant, and membrane LPG isolated from metacyclic promastigotes of Leishmania major. The purification scheme included anion-exchange chromatography, hydrophobic interaction chromatography and cold methanol precipitation. The purity of supernatant LPG (sLPG) and membrane LPG (mLPG) was determined by SOS-PAGE and thin layer chromatography. The effect of mLPG and sLPG on nitric oxide (NO) production by murine macrophages cell line (J77 4.1 A) was studied. Both sLPG and mLPG induced NO production in a dose dependent manner but sLPG induced significantly higher amount of NO than mLPG. Our results show that sLPG is able to promote NO production by murine macrophages.

• Membrane and Water Treatment
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• 제7권5호
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• pp.451-462
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• 2016

The molecular weight of proteins and protein hydrolysis products (PHP) in the fractionated post-production marinating brines left after herring marination process was determined by the HPLC. The proteins and PHP retention was evaluated in the three-stage purification process with the usage of polypropylene bag ($25{\mu}m$) and ceramic membranes with the cut-off of 150 and 1 kDa. It was found that the process of marination contributes to high participation of compounds in the post-production marinating brines. Those are characterised by low molecular weight, formed as a result of protein hydrolysis. Each stage of the scavenging process was reducing the content of proteins and PHP. The lowest retention was observed in the stage at which a PP bag was used, while the highest in the UF process, with the usage of 150 kDa membrane. The total retention of proteins and PHP differed according to the type of post-production marinating brines and reached the level of 16-22%.