• IMMUNE NETWORK
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• 제14권6호
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• pp.321-327
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• 2014

TGF-${\beta}$ induces IgA class switching by B cells. We previously reported that Smad3 and Smad4, pivotal TGF-${\beta}$ signal-transducing transcription factors, mediate germline (GL) ${\alpha}$ transcription induced by TGF-${\beta}1$, resulting in IgA switching by mouse B cells. Post-translational sumoylation of Smad3 and Smad4 regulates TGF-${\beta}$-induced transcriptional activation in certain cell types. In the present study, we investigated the effect of sumoylation on TGF-${\beta}1$-induced, Smad3/4-mediated $GL{\alpha}$ transcription and IgA switching by mouse B cell line, CH12F3-2A. Overexpression of small ubiquitin-like modifier (SUMO)-1, SUMO-2 or SUMO-3 did not affect TGF-${\beta}1$-induced, Smad3/4-mediated $GL{\alpha}$ promoter activity, expression of endogenous $GL{\alpha}$ transcripts, surface IgA expression, and IgA production. Next, we tested the effect of the E3 ligase PIASy on TGF-${\beta}1$-induced, Smad3/4-mediated $GL{\alpha}$ promoter activity. We found that PIASy overexpression suppresses the $GL{\alpha}$ promoter activity in cooperation with histone deacetylase 1. Taken together, these results suggest that SUMO itself does not affect regulation of $GL{\alpha}$ transcription and IgA switching induced by TGF-${\beta}1$/Smad3/4, while PIASy acts as a repressor.

• IMMUNE NETWORK
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• 제14권3호
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• pp.128-137
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• 2014

Respiratory viruses can induce acute respiratory disease. Clinical symptoms and manifestations are dependent on interactions between the virus and host immune system. Dendritic cells (DCs), along with alveolar macrophages, constitute the first line of sentinel cells in the innate immune response against respiratory viral infection. DCs play an essential role in regulating the immune response by bridging innate and adaptive immunity. In the steady state, lung DCs can be subdivided into $CD103^+$ conventional DCs (cDCs), $CD11b^+$ cDCs, and plasmacytoid DCs (pDCs). In the inflammatory state, like a respiratory viral infection, monocyte-derived DCs (moDCs) are recruited to the lung. In inflammatory lung, discrimination between moDCs and $CD11b^+$ DCs in the inflamed lung has been a critical challenge in understanding their role in the antiviral response. In particular, $CD103^+$ cDCs migrate from the intraepithelial base to the draining mediastinal lymph nodes to primarily induce the $CD8^+$ T cell response against the invading virus. Lymphoid $CD8{\alpha}^+$ cDCs, which have a developmental relationship with $CD103^+$ cDCs, also play an important role in viral antigen presentation. Moreover, pDCs have been reported to promote an antiviral response by inducing type I interferon production rather than adaptive immunity. However, the role of these cells in respiratory infections remains unclear. These different DC subsets have functional specialization against respiratory viral infection. Under certain viral infection, contextually controlling the balance of these specialized DC subsets is important for an effective immune response and maintenance of homeostasis.

• 문화기술의 융합
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• 제6권4호
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• pp.123-130
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• 2020

쥬얼리 CAD, CAM 등의 자동화 시스템이 도입되기 이전의 쥬얼리 제조 산업은 핸드메이드, 즉 손으로 직접 만드는 방식의 원본 제작이 많았다. 그러나 자동화 시스템의 도입이후 고급기술을 가진 원본 세공기사들은 사라지고 컴퓨터디자이너와 3D프린팅 출력 기사들이 그 자리를 대신하는 추세이다. 기존의 헨드 메이드 방식의 생산라인에서 CAD, CAM 등 자동화 시스템의 도입은 디자인의 다양화, 쥬얼리 제조의 첨단화, 전문화를 이루었다. 국내 쥬얼리 산업에서 제작 공정 시 CAD, CAM등의 자동화 시스템의 의존도가 지속적으로 높아지고 있으며 대학의 쥬얼리디자인 교육과정에서도 점점 많은 부분을 차지하고 있는 실정이다. 이러한 결과로 볼 때 앞으로 쥬얼리 제조 산업에서의 CAD/CAM 자동화 시스템분야는 꼭 필요한 중요한 분야가 되고 있다.

• 대한임상독성학회지
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• 제15권2호
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• pp.107-115
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• 2017

Purpose: Glehnia littoralis has been used to treat ischemic stroke, phlegm, cough, systemic paralysis, antipyretics and neuralgia. The pharmacological mechanisms of Glehnia littoralis include calcium channel block, coumarin derivatives, anticoagulation, anti-convulsive effect, as well as anti-oxidant and anti-inflammatory effects. Alpha-amanitin (${\alpha}$-amanitin) is a major toxin from extremely poisonous Amanita fungi. Oxidative stress, which may contribute to severe hepatotoxicity was induced by ${\alpha}$-amanitin. The aim of this study was to investigate whether Glehnia littoralis ethyl acetate extract (GLEA) has the protective antioxidant effects on ${\alpha}$-amanitin -induced hepatotoxicity. Methods: Human hepatoma cell line HepG2 cells were pretreated in the presence or absence of GLEA (50, 100 and $200{\mu}g/ml$) for 4 hours, then exposed to $60{\mu}mol/L$ of${\alpha}$-amanitin for an additional 4 hours. Cell viability was evaluated using the MTT method. AST, ALT, and LDH production in a culture medium and intracellular MDA, GSH, and SOD levels were determined. Results: GLEA (50, 100 and $200{\mu}g/ml$) significantly increased the relative cell viability by 7.11, 9.87, and 14.39%, respectively, and reduced the level of ALT by 10.39%, 34.27%, and 52.14%, AST by 9.89%, 15.16%, and 32.84%, as well as LDH by 15.86%, 22.98%, and 24.32% in culture medium, respectively. GLEA could also remarkably decrease the level of MDA and increase the content of GSH and SOD in the HepG2 cells. Conclusion: In the in vitro model, Glehnia littoralis was effective in limiting hepatic injury after ${\alpha}$-amanitin poisoning. Its antioxidant effect is attenuated by antidotal therapy.

• 농업과학연구
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• 제2권1호
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• pp.265-279
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• 1975

This study was conducted to investigate the development of Gab river-basin which was a part of the farm land enlargement to contribute to the increased production of food. The results were as follows; 1. Gab river has the upper stream which occupy 50 percent in moumtains and the mid-stream in the Daejeon city area, and the downstream in a field which is about 22.9 percent in which farming area per household is 0.82 ha., agricultural population is 76 percent except of Daejeon city. Also, urban enlargement of mid-stream basin and development of industrial area in the lower stream diminish farm land. Consequently, this area should be developed to farm land to increase farming size. 2. There is no possibility to develop farm land in mountains of which (64.9 percent) is forests and in midstream which was constructed river-improvement. But Weonjeong area and Yongcheon area will be effective area. 3. If river banks of Weonjeong area will make straight with cost of construction 195,000,000 won, bank length 6 km will be useless, water will flow smoothly, flood will be prevented, farm land will develop 21.66 ha in which rice will produce annually 81.698 M/T which is about 10,860,000 won. 4. This area has good conditions of development. that is, investment efficiency (B/C) is 1.47 more than 1.00. 5. This area is a multiple purpose development district. The reasons are that there are beautiful mountains and a reservoir to be expected to construct, so it will be a sight seeing district in the vicinity of Daejeon city. 6. If Honam railway double line and river straight construction had executed simultaneously, cost of construction 50,000,000 won would have saved.

• 천문학논총
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• 제30권2호
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• pp.605-607
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• 2015

The development of time-frequency analysis techniques allow astronomers to successfully deal with the non-stationary time series that originate from unstable physical mechanisms. We applied a recently developed time-frequency analysis method, the Hilbert-Huang transform (HHT), to two non-stationary phenomena: the superorbital modulation in the high-mass X-ray binary SMC X-1 and the quasi-periodic oscillation (QPO) of the AGN RE J1034+396. From the analysis of SMC X-1, we obtained a Hilbert spectrum that shows more detailed information in both the time and frequency domains. Then, a phase-resolved analysis of both the spectra and the orbital profiles was presented. From the spectral analysis, we noticed that the iron line production is dominated by different regions of this binary system in different superorbital phases. Furthermore, a pre-eclipse dip lying at orbital phase ~0:6-0:85 was discovered during the superorbital transition state. We further applied the HHT to analyze the QPO of RE J1034+396. From the Hilbert spectrum and the O-C analysis results, we suggest that it is better to divide the evolution of the QPO into three epochs according to their different periodicities. The correlations between the QPO periods and corresponding fluxes were also different in these three epochs. The change in periodicity and the relationships could be interpreted as the change in oscillation mode based on the diskoseismology model.

• The Journal of Advanced Prosthodontics
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• 제1권1호
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• pp.56-61
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• 2009

STATEMENT OF PROBLEM. The success of titanium implants is due to osseointegration or the direct contact of the implant surface and bone without a fibrous connective tissue interface. PURPOSE. The purpose of this study was to evaluate the osteoblast precursor response to titanium-10 tantalum-10 niobium(Ti-Ta-Nb) alloy and its sputtered coating. MATERIAL AND METHODS. Ti-Ta-Nb coatings were sputtered onto the Ti-Ta-Nb disks. Ti6-Al-4V alloy disks were used as controls. An osteoblast precursor cell line, were used to evaluate the cell responses to the 3 groups. Cell attachment was measured using coulter counter and the cell morphology during attachment period was observed using fluorescent microscopy. Cell culture was performed at 4, 8, 12 and 16 days. RESULTS. The sputtered Ti-Ta-Nb coatings consisted of dense nanoscale grains in the range of 30 to 100 nm with alpha-Ti crystal structure. The Ti-Ta-Nb disks and its sputtered nanoscale coatings exhibited greater hydrophilicity and rougher surfaces compared to the Ti-6Al-4V disks. The sputtered nanoscale Ti-Ta-Nb coatings exhibited significantly greater cell attachment compared to Ti-6Al-4V and Ti-Ta-Nb disks. Nanoscale Ti-Ta-Nb coatings exhibited significantly greater ALP specific activity and total protein production compared to the other 2 groups CONCLUSIONS. It was concluded that nanoscale Ti-Ta-Nb coatings enhance cell adhesion. In addition, Ti-Ta-Nb alloy and its nanoscale coatings enhanced osteoblast differentiation, but did not support osteoblast precursor proliferation compared to Ti-6Al-4V. These results indicate that the new developed Ti-Ta-Nb alloy and its nanoscale Ti-Ta-Nb coatings may be useful as an implant material.

• 대한조선학회논문집
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• 제34권1호
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• pp.50-59
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• 1997

선박의 시운전 수행 이전에 엔진 및 프로펠러의 작동상태를 체크하기 위하여 때때로 안벽계류 중에서 주기 및 발전기의 성능시험을 수행하는 데, 이때 주기관을 100% 작동시키는 것은 계류용 로프 및 안벽 비트의 강도 문제등의 여러가지 위험 부담을 갖게 된다. 본 연구는 이러한 시험에 대한 안전성 검토를 위해 필요한 프로펠러의 추력의 추정을 목적으로 하고 있다. 대상선은 최근에 건조한 대형 컨테이너선이며, 실선시험은 안벽계류 상태에서 수행되었기 때문에 프로펠러는 수면위로 노출되어 있는 상태에서 작동되었다. 이러한 낮은 프로펠러 축 몰수 수심에서 작동하는 안벽계류 상태에서의 프로펠러의 특성을 조사하기 위해 모형시험을 수행하였으며, 이들 시험결과로부터 안벽계류 시험중의 프로펠러의 추력에 관한 약산식이 도출되었다. 그 결과 프로펠러의 특성은 축 수심에 따라 크게 달라지는 것을 알 수 있으며, 특히 축 수심이 작아 프로펠러가 수면위에 노출되어 있는 경우에는, 같은 축 수성에서도 프로펠러 회전수에 지배되는 특성이 파악되었다. 한편 심수 중에서 작동하는 프로펠러에 대해서도 추력에 대한 추정식을 도출하여 실선 시험 담당자가 사용하기 쉽게 하였으며, bollard pull에서의 FPSO(Floating Production Storage and Offloading tanker based system) 의 프로펠러의 추력 계측값과 비교 검토 하였다.

• 한국측량학회지
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• 제22권3호
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• pp.277-284
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• 2004

본 연구는 접경지역에 인접한 지역의 특성상 항공사진 촬영이 불가능한 지역 중 파주시에 대하여 1m 고해상도 스테레오 영상을 이용하여 1/5,000 대축척 수치지도 제작에 시험 적용한 연구이다. 위성영상의 정밀기하보정을 위하여 GPS 관측에 의하여 획득된 지상기준점을 이용하였으며, IKONOS 스테레오 영상과 디지털 해석도화기를 이용하여 수치지도를 제작하였다. 제작된 수치지도에 대하여 GPS를 이용한 현지 검사점 측량을 실시한 결과 평면과 수직위치의 RMSE는 각각 $\pm$1.706m와 $\pm$1.231m로 관측되었다. 이 수평과 수직위치 RMSE는 NGIS 수치지도의 정확도를 만족하는 값으로써 본 연구를 통하여 제안된 새로운 방법은 군사적 원인으로 인한 비행금지구역의 대축척 수치지도 제작을 위한 좋은 기회라고 할 수 있다.

• 한국광물학회지
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• 제22권4호
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• pp.353-358
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• 2009

CRT 브라운관 패널의 연마과정에서 배출되는 폐연마슬러지로부터 석류석 등의 연마재를 회수하고, 연마성능을 평가하였다. 재활용 부석과 석류석으로 연마한 유리의 평균표면거칠기값(Ra = $0.025{\mu}m$, = $0.029{\mu}m$)은 새 연마재로 연마한 유리에서의 값(Ra = $0.039{\mu}m$, $0.031{\mu}m$)보다 작았으며 표면의 선이나 홈도 적었다. 루즈의 경우 유리 파편이 혼입되어 연마재로 재활용하기에는 적합하지 않다. 본 기술을 이용하여 폐연마슬러지에 함유된 천연연마재 광물자원인 부석과 석류석을 회수한다면 CRT 유리 연마공정에서 재활용할 수 있고, 슬러지 매립으로 발생되는 처리비용과 환경오염문제를 줄일 수 있을 것이다.