Journal of The Korean Society of Clinical Toxicology (대한임상독성학회지)

Korean society of Clincal Toxicology (대한임상독성학회)
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In vitro Protective Effects of Glehnia Littoralis on Alpha-amanitin Induced Hepatotoxicity

알파 아마니틴에 의한 간독성에 대한 갯방풍의 보호 효과

  • Kim, Bo Hyun (Department of Emergency Medicine, School of Medicine, Chosun University) ;
  • Sun, Kyung Hoon (Department of Emergency Medicine, School of Medicine, Chosun University) ;
  • Kim, Sun Pyo (Department of Emergency Medicine, School of Medicine, Chosun University) ;
  • Park, Yongjin (Department of Emergency Medicine, School of Medicine, Chosun University)
  • 김보현 (조선대학교 의과대학 응급의학교실) ;
  • 선경훈 (조선대학교 의과대학 응급의학교실) ;
  • 김선표 (조선대학교 의과대학 응급의학교실) ;
  • 박용진 (조선대학교 의과대학 응급의학교실)
  • Received : 2017.10.15
  • Accepted : 2017.10.24
  • Published : 2017.12.31
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Abstract

Purpose: Glehnia littoralis has been used to treat ischemic stroke, phlegm, cough, systemic paralysis, antipyretics and neuralgia. The pharmacological mechanisms of Glehnia littoralis include calcium channel block, coumarin derivatives, anticoagulation, anti-convulsive effect, as well as anti-oxidant and anti-inflammatory effects. Alpha-amanitin (${\alpha}$-amanitin) is a major toxin from extremely poisonous Amanita fungi. Oxidative stress, which may contribute to severe hepatotoxicity was induced by ${\alpha}$-amanitin. The aim of this study was to investigate whether Glehnia littoralis ethyl acetate extract (GLEA) has the protective antioxidant effects on ${\alpha}$-amanitin -induced hepatotoxicity. Methods: Human hepatoma cell line HepG2 cells were pretreated in the presence or absence of GLEA (50, 100 and $200{\mu}g/ml$) for 4 hours, then exposed to $60{\mu}mol/L$ of${\alpha}$-amanitin for an additional 4 hours. Cell viability was evaluated using the MTT method. AST, ALT, and LDH production in a culture medium and intracellular MDA, GSH, and SOD levels were determined. Results: GLEA (50, 100 and $200{\mu}g/ml$) significantly increased the relative cell viability by 7.11, 9.87, and 14.39%, respectively, and reduced the level of ALT by 10.39%, 34.27%, and 52.14%, AST by 9.89%, 15.16%, and 32.84%, as well as LDH by 15.86%, 22.98%, and 24.32% in culture medium, respectively. GLEA could also remarkably decrease the level of MDA and increase the content of GSH and SOD in the HepG2 cells. Conclusion: In the in vitro model, Glehnia littoralis was effective in limiting hepatic injury after ${\alpha}$-amanitin poisoning. Its antioxidant effect is attenuated by antidotal therapy.

Keywords

References

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