• Journal of Chitin and Chitosan
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• v.22 no.3
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• pp.185-193
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• 2017

Red tide Heterosigma akashiwo (H. akashiwo), a microscopic alga of the class Raphidophyceae, causes extensive damage to all marine ecosystems. It is essential to reduce the damage to marine ecosystems for them to be used as a resource. In this study, we used organic solvent fractionation to obtain an ethyl acetate-methanol extract from H. akashiwo (HAEM80) and then evaluated its anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and a zebrafish model. HAME80 markedly inhibited the production of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$). It also down-regulated the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and decreased the secretion of interleukin-$1{\beta}$ ($IL-1{\beta}$) in LPS-stimulated RAW 264.7 cells. HAME80 reduced yolk edema and improved the survival rate of LPS-stimulated zebrafish embryos; in addition, the extract significantly reduced the production of ROS and NO and attenuated cell death in this model. Gas chromatography-mass spectrometry (GC-MS) of the extract was used to confirm the identity of peaks 1-20. Taken together, our data suggest that H. akashiwo is a beneficial anti-inflammatory agent.

• Journal of The Korean Society of Agricultural Engineers
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• v.63 no.5
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• pp.83-93
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• 2021

Agricultural reservoirs are important facilities for storing or managing water for the purpose of securing agricultural water, creating and expanding agricultural production bases, and using them to increase agricultural production. In particular, the Korea Rural Community Corporation (KRC) manages agricultural reservoirs scattered across the country, and officially recognizes and distributes hydrological data to increase their public utilization and aims to improve the value of water resources. Data on the water level of agricultural reservoirs are important. However, errors such as missing values and outliners limit utilization of the data in various fields of research and industry. Therefore, water quality data measures should be devised to increase reliability. this study categorized different error types and looked at automatic correction methods to enhance the reliability of the vast hydrological data. In addition, the water level data corrected from errors were compared to the reference hydrologic data through expert judgment in accordance with the quality control procedure, and the most appropriate measures were verified. As KRC manages more agricultural reservoirs than any other institution, the proposed method of efficient and automatic water level data correction in this study is expected to increase the availability and reliability of the hydrological data.

• International Journal of Computer Science & Network Security
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• v.22 no.7
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• pp.443-450
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• 2022

The method of studying 3D-technologies abroad and in Ukraine is considered. The analysis of educational resources and experience of use of the equipment in branch is carried out. The didactic principles of the educational process for 3D-printing specialists are determined. The use of FDM technology and the ability to minimize the occurrence of defects in the future have been studied. An analysis of the international experience of the educational process of relevant specialists in the field. The content of training for 3D printing specialists has been developed. The experience of using 3D-technologies is described and the list of recommendations for elimination of defects during production of products by means of additive technologies is made. The recommendations will be useful not only for beginners, but also for experienced professionals in additive technologies. The need to study such experience is the main condition for the development of enterprises in Ukraine that plan to automate their own production. A 3D printing engineer must know the basics of economics and marketing, because his responsibilities include optimizing workflows to reduce the cost and speed up printing. Therefore, the knowledge gained from practical experience presented and in building for learning 3D printing engineers by the authors will be important.

• Journal of Information Technology Applications and Management
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• v.30 no.1
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• pp.115-126
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• 2023

In various fields such as art, design, music, film, sports, games, and fashion, NFTs (Non-Fungible Tokens) are creating new economic value through trading platforms dedicated to NFT art and content. In this article, I analyze the current state of blockchain technology and NFT art content in the context of an expanding market for blockchain-based NFT art content in the metaverse. I also propose several tasks based on the economic and industrial logic of technological innovation. The first task proposed is to integrate cultural arts on blockchain, metaverse, and NFT platforms through digital innovation, instead of separating or distinguishing between creative production and consumption. Before the COVID-19 pandemic, there was a clear separation between creators and consumers. However, with the rise of Web 3.0 platforms, any user can now create and own their own content. Therefore, it is important to promote a collaborative and integrated approach to cultural arts production and consumption in the blockchain and metaverse ecosystem. The second task proposed is to align the legal framework with blockchain-based technological innovation. The enactment and revision of relevant laws should focus on promoting the development of the NFT trading platform ecosystem, rather than merely regulating it for user protection. As blockchain-based technology continues to evolve, it is important that legal systems adapt to support and promote innovation in the space. This shift in focus can help create a more conducive environment for the growth of blockchain-based NFT platforms. The third task proposed is to integrate education on digital arts, including metaverse and NFT art contents, into the current curriculum. This education should focus on convergence and consilience, rather than merely mixing together humanities, technology, and arts. By integrating digital arts education into the curriculum, students can gain a more comprehensive understanding of the potential of blockchain-based technologies and NFT art. This article examines the digital technological innovation such as blockchain, metaverse, and NFT from an economic and industrial point of view. As a limitation of this research, the critical mind such as philosophical thinking or social criticism on technological innovation is left as a future task.

• The Journal of the Convergence on Culture Technology
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• v.9 no.1
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• pp.191-197
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• 2023

The purpose of the study was to provide an educational environment for designing and producing virtual reality practice contents that can be used in semiconductor production lines and clean rooms. Through this process, the user can acquire practical knowledge through experiences close to reality, such as experiencing the main semiconductor solar cell manufacturing facilities as well as procedural knowledge before and after entering the clean room.. In particular, it provides users with an immersion experience close to reality by creating an environment for experiential content necessary for semiconductor and solar cell manufacturing processes and clean room entrance procedure experiential content, which is expected to improve education immersion, realism, cost, efficiency, and education satisfaction. Depending on the characteristics of Dangerous, Impossible, Counter-productive etc, immersive content makes learners immersed in the learning content, induces proactive/active learning, and embodies the learning content, resulting in positive results in the field of improving educational effectiveness.

• IMMUNE NETWORK
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• v.20 no.6
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• pp.49.1-49.15
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• 2020

C-C chemokine receptor type 5 (CCR5) regulates the trafficking of various immune cells to sites of infection. In this study, we showed that expression of CCR5 and its ligands was rapidly increased in the kidney after systemic Candida albicans infection, and infected CCR5^-/- mice exhibited increased mortality and morbidity, indicating that CCR5 contributes to an effective defense mechanism against systemic C. albicans infection. The susceptibility of CCR5^-/- mice to C. albicans infection was due to impaired fungal clearance, which in turn resulted in exacerbated renal inflammation and damage. CCR5-mediated recruitment of NK cells to the kidney in response to C. albicans infection was necessary for the anti-microbial activity of neutrophils, the main fungicidal effector cells. Mechanistically, C. albicans induced expression of IL-23 by CD11c⁺ dendritic cells (DCs). IL-23 in turn augmented the fungicidal activity of neutrophils through GM-CSF production by NK cells. As GM-CSF potentiated production of IL-23 in response to C. albicans, a positive feedback loop formed between NK cells and DCs seemed to function as an amplification point for host defense. Taken together, our results suggest that CCR5-mediated recruitment of NK cells to the site of fungal infection is an important step that underlies innate resistance to systemic C. albicans infection.

• Journal of Applied Biological Chemistry
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• v.57 no.4
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• pp.373-377
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• 2014

In this study, we demonstrated whether the extract of Fomes fomentarius (FFE; FF extract) could be used to stimulate macrophages (RAW 264.7 cells). All four doses of FFE (5, 10, 20, and $40{\mu}g/mL$) had no significant cytotoxicity during the entire experimental period. FFE potently increased the production of nitric oxide (NO). Consistent with these observations, inducible NO synthase levels were increased by FFE in a dose-dependent manner. Moreover, FFE increased the production of tumor necrosis factor-${\alpha}$, interleukin (IL)-$1{\beta}$, and IL-6 in the same cells. These stimulating effects of FFE were found to be caused by the stimulation of phosphorylation of $I{\kappa}B{\alpha}$ and MAP kinases (p38, ERK, and JNK). These results suggest that FFE may be used as new agents for wide application in the immune study of mushroom.

• Journal of The Korean Association of Information Education
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• v.21 no.4
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• pp.451-462
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• 2017

This study selected 3D printing that is highly likely to be adopted in schools. This research was conducted in two stages: 1) proposing the learning activity framework for utilizing 3D printing in education, and 2) exploring the potential of integrating 3D printing in the school field. The '3D printing learning activity framework' proposed in this study includes four phases that are categorized according to the complexity of problem-solving processes and collaborative interaction: Step 1 as production through replication, Phase 2 as means of imaginary expression, Phase 3 as near problem-solving, and Phase 4 as expanded problem-solving. Next, we conducted the field study with 23 students in the 6th grade math class where they learned the various solid shapes and volumes through 3D printing-integrated activities. The lesson was considered as Phase 1, which is the production through replication. Overall, the results showed that the participants had positive perceptions about the efficacy of 3D printing activities, the quality of learning experience, and satisfaction. On the other hand, it was found that the usability of 3D printers and CAD program needs further improvement The contribution of this study can be found in the learning activity framework that can guide 3D printing activity design in school, and in the exploration of enhancing the connection between 3D printing activities and curricular relevance beyond simple interest toward a novel technology.

• The Korean Journal of Mycology
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• v.30 no.1
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• pp.61-65
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• 2002

The objectives of this study were to find the nuruk fungal isolate for Korean traditional rice wine and to compare the tastes of rice wines made with its single inoculum. The fungal isolates including the species of Absidia, Mucor, Rhizopus and Aspergillus were used for Korean traditional rice-wine production. The four species were cultured and measured for productions of amylase under various water contents. The four different species tested were different in their growth under the rice cereals with different moisture contents; the species of Absidia and Rhizopus grew better compared to the other species under the lower moisture conditions. The tastes and alcohol concentrations of the rice-wines made with each different inoculum were compared with those of commercial rice wines made with traditional method. As a result, it was speculated that the traditional rice wines were made with nuruk, which is mainly composed of Zygomycetous fungi, such as Absidia, Mucor, and Rhzopus. Also, it was strongly suggested that the traditional nuruk might not be a source of amylase, but a source of fungal inoculum.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.52 no.3
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• pp.241-247
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• 2016

The trawl fishery is an important fishery accounting for around 30.0% of adjacent water fishery production in Korea, and the commercial fishing is associated with high rate of fatal and non-fatal occupational injury. The hazard analysis for the fishermen's safety of offshore trawler was conducted to serve as basic data for improving the healthy and safe working environment of fishermen using fishermen's occupational accidents of the national federation of fisheries cooperatives (NFFC) from 2012 to 2014 (n=464). As a result, the occupational accident occurrence rate of large powered trawl fishery was 241.4‰ in east sea trawl fishery, 6.9 times the rate of that. In addition, death and missing rate was found to have very serious level management to 97.7‱ in east sea trawl fishery of death at 6.2 times. The accident occurred in 91.7 to 100.0% was happened at sea. The slipping, others and struck by object and son on occurred more frequently in order in the frequency of accident occurrence pattern. However, the occurrence rate of death and missing did not match the frequency of accident pattern. In other words, slipping occurred more frequently while death and missing risk was not high. The fall in the waters was low while death and missing risk was high. The results are expected to help identify and assess safety hazard occurred in offshore trawlers.