Journal of Chitin and Chitosan (한국키틴키토산학회지)

The Korean Society for Chitin and Chitosan (한국키틴키토산학회)
권호 표지

Anti-inflammatory Activity on LPS-stimulated in vitro RAW 264.7 Cells and in vivo Zebrafish of Heterosigma akshiwo

  • Kim, Junseong (Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology (KIOST)) ;
  • Choi, Youn Kyung (Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology (KIOST)) ;
  • Lee, Ji-Hyeok (Department of Marine Life Science, Jeju National University) ;
  • Kim, Seo-Young (Department of Marine Life Science, Jeju National University) ;
  • Kim, Hyun-Soo (Department of Marine Life Science, Jeju National University) ;
  • Jeon, You-Jin (Department of Marine Life Science, Jeju National University) ;
  • Heo, Soo-Jin (Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology (KIOST))
  • Received : 2017.09.07
  • Accepted : 2017.09.26
  • Published : 2017.09.30

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Abstract

Red tide Heterosigma akashiwo (H. akashiwo), a microscopic alga of the class Raphidophyceae, causes extensive damage to all marine ecosystems. It is essential to reduce the damage to marine ecosystems for them to be used as a resource. In this study, we used organic solvent fractionation to obtain an ethyl acetate-methanol extract from H. akashiwo (HAEM80) and then evaluated its anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and a zebrafish model. HAME80 markedly inhibited the production of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$). It also down-regulated the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and decreased the secretion of interleukin-$1{\beta}$ ($IL-1{\beta}$) in LPS-stimulated RAW 264.7 cells. HAME80 reduced yolk edema and improved the survival rate of LPS-stimulated zebrafish embryos; in addition, the extract significantly reduced the production of ROS and NO and attenuated cell death in this model. Gas chromatography-mass spectrometry (GC-MS) of the extract was used to confirm the identity of peaks 1-20. Taken together, our data suggest that H. akashiwo is a beneficial anti-inflammatory agent.

Keywords

Acknowledgement

Supported by : Ministry of Oceans and Fisheries, Korea Institute of Ocean Science and Technology

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