Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Immune-stimulatory Effects of Fomes fomentarius Extract in Murine Macrophages

말굽버섯 추출물의 대식세포 면역반응 증강 효과

  • Kim, Young Hoon (Department of Biology Education, College of Education, Chungbuk National University) ;
  • Park, Eun Gyu (Department of Biology Education, College of Education, Chungbuk National University) ;
  • Batsuren, Dulamjav (Institutes of Chemistry and Chemical Technology IV Build. MAS) ;
  • Ganbaatar, Jamsranjav (Institutes of Chemistry and Chemical Technology IV Build. MAS) ;
  • Nho, Chu Won (Natural Products Research Center, Korea Institute of Science and Technology (KIST)) ;
  • Pan, Cheol-Ho (Natural Products Research Center, Korea Institute of Science and Technology (KIST)) ;
  • Lee, Jae Kwon (Department of Biology Education, College of Education, Chungbuk National University)
  • Received : 2014.08.05
  • Accepted : 2014.09.11
  • Published : 2014.12.31
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Abstract

In this study, we demonstrated whether the extract of Fomes fomentarius (FFE; FF extract) could be used to stimulate macrophages (RAW 264.7 cells). All four doses of FFE (5, 10, 20, and $40{\mu}g/mL$) had no significant cytotoxicity during the entire experimental period. FFE potently increased the production of nitric oxide (NO). Consistent with these observations, inducible NO synthase levels were increased by FFE in a dose-dependent manner. Moreover, FFE increased the production of tumor necrosis factor-${\alpha}$, interleukin (IL)-$1{\beta}$, and IL-6 in the same cells. These stimulating effects of FFE were found to be caused by the stimulation of phosphorylation of $I{\kappa}B{\alpha}$ and MAP kinases (p38, ERK, and JNK). These results suggest that FFE may be used as new agents for wide application in the immune study of mushroom.

본 연구에서는 아직 까지 연구되지 않은 말굽버섯의 면역증강 효과를 설치류 대식세포에서 검증하였다. 연구결과에 따르면 말굽버섯 메탄올 추출물(FFE)의 처리에 의해 대식세포의 산화질소의 생성이 농도 의존적으로 증가되었다. 산화질소의 생성이 증가된 이유는 산호질소의 생성을 유도하는 효소인 iNOS의 발현이 FFE에 의해 증가되었기 때문이었다. FFE는 면역반응에 중요한 cytokine인 TNF-${\alpha}$, IL-$1{\beta}$, IL-6의 생성도 농도 의존적으로 증가 시켰다. 이 같은 FFE의 면역증강 활성은 면역활성을 중계하는 세포 신호전달분자 중 NF-${\kappa}B$와 MAP Kinases의 활성증가에 의한 것임을 확인 할 수 있었다. 본 연구 결과는 말굽버섯의 임상적 적용에 중요한 자료로 활용될 것이며, 만일 말굽버섯으로부터 분리한 단일성분에서 면역증강활성을 검증 하게 된다면 새로운 식 의약소재로서의 가치를 인정 받을 수 있을 것으로 생각된다.

Keywords

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