• Applied Biological Chemistry
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• 제39권2호
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• pp.112-117
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• 1996

Arginine decarboxylase (ADC) is the first enzyme in one of the two pathways of diamine putrescine biosynthesis in plants. The genes encoding ADC have previously been cloned from Escherichia coli, oat and tomato genome. Two degenerate oligonucleotides (17-mer) corresponding to two conserved regions of ADC were used as primers in polymerase chain reaction of rice (Oryza sativa L.) genomic DNA, and an approximately 1.0 kbp fragment was obtained. This amplified PCR product showed an open reading frame which contains 1,022 bp of nucleotide sequences. This PCR product was cloned into pGEM-originated T vector and the short 500 bp PstI digested fragment was subcloned into pGEM-3zf(+/-) vectors to facilitate sequencing. The nucleotide sequence of this PCR product showed about 74% and 70% identity with the same regions of the oat and tomato ADC cDNA sequences, respectively. The predicted amino acid sequence exhibited 45% and 62% identity with oat and tomato ADC polypeptide fragments, respectively. The sequence similarities of 34%, 47% and 38% were previously reported in oat and E. coli, tomato and oat, and tomato and E. coli ADC amino acids, respectively. Therefore, similarities and identities between rice and oat or tomato are remarkably higher than those others of the previous reports. In the highly conserved regions in both the amino acid sequence and spacing regions among the sequences of these three, rice ADC open reading frame also has the exactly same regions with the striking similarity. RNA blot analysis showed that hnc is expressed as a transcript of approximately 2.5 kbP in the rice seedling leaf tissues.

• International Journal of Computer Science & Network Security
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• 제21권6호
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• pp.161-168
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• 2021

Within the article, strategic guidelines for the modernization of microeconomic systems are identified. Modernization levels of the potential implementation are formalized for enterprises: contractile, extensive technical, technological, progressive, adaptive, steady, intensive, creative, absolute and leader modernization. This allowed to specify the directions and tasks of the enterprise modernization at different management levels. Accordingly, the conditions and criteria for selecting resource tools are set. It is proved that the strategies of the potential modernization of enterprises must be carried out at four main management levels: first, at the enterprise level; secondly, for a particular type of product / service; third, by functional directions of modernization of separate spheres of the enterprise activity or responsibility, fourth, at the level of structural units of the enterprise. It is substantiated that in the processes due to the activation of the potential modernization, the resources are transformed into the results of the innovation implementation and the investment strategies modernization. A system of tasks for the corporate strategies implementation in order to modernize microeconomic systems has been formed. Key vectors of the activation determine the nature and properties of investment resources and necessary innovations to enhance the modernization potential. Therefore, the system of innovation and investment strategies' modernization, based on the vector and resource provision of the modernization process, is specified:

• Journal of Power System Engineering
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• 제17권1호
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• pp.50-57
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• 2013

This describes the formulation for the free vibration of joined conical-cylindrical shells with uniform thickness using the transfer of influence coefficient. This method was developed based on successive transmission of dynamic influence coefficients, which were defined as the relationships between the displacement and the force vectors at arbitrary nodal circles of the system. The two edges of the shell having arbitrary boundary conditions are supported by several elastic springs with meridional/axial, circumferential, radial and rotational stiffness, respectively. The governing equations of vibration of a conical shell, including a cylindrical shell, are written as a coupled set of first order differential equations by using the transfer matrix of the shell. Once the transfer matrix of a single component has been determined, the entire structure matrix is obtained by the product of each component matrix and the joining matrix. The natural frequencies and the modes of vibration were calculated numerically for joined conical-cylindrical shells. The validity of the present method is demonstrated through simple numerical examples, and through comparison with the results of previous researchers.

• Journal of Power Electronics
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• 제18권3호
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• pp.789-801
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• 2018

Model predictive direct power control (MPDPC) is a widely recognized high-performance control strategy for a three-phase grid-connected pulse width modulation (PWM) rectifier. Unlike those of conventional grid-connected PWM rectifiers, the active and reactive powers of permanent magnet synchronous generator (PMSG)-connected PWM rectifiers, which are used in electromagnetic transmitters, cannot be calculated as the product of voltage and current because the back electromotive force (EMF) of the generator cannot be measured directly. In this study, the predictive power model of the rectifier is obtained by analyzing the relationship among flux, back EMF, active/reactive power, converter voltage, and stator current of the generator. The concept of duty cycle control in the proposed MPDPC is introduced by allocating a fraction of the control period for a nonzero vector and rest time for a zero vector. When nonzero vectors and their duration in the predefined cost function are simultaneously evaluated, the global power ripple minimization is obtained. Simulation and experimental results prove that the proposed MPDPC strategy with duty cycle control for the PMSG-connected PWM rectifier can achieve better control performance than the conventional MPDPC-SVM with grid-connected PWM rectifier.

• Journal of Microbiology and Biotechnology
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• 제21권8호
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• pp.854-857
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• 2011

POMT7, which is an O-methyltransferase from poplar, transfers a methyl group to several flavonoids that contain a 7-hydroxyl group. POMT7 has been shown to have a higher affinity toward quercetin, and the reaction product rhamnetin has been shown to inhibit the formation of beta-amyloid. Thus, rhamnetin holds great promise for use in therapeutic applications; however, methods for mass production of this compound are not currently available. In this study, quercetin was biotransformed into rhamnetin using Escherichia coli expressing POMT7, with the goal of developing an approach for mass production of rhamnetin. In order to maximize the production of rhamnetin, POMT7 was subcloned into four different E. coli expression vectors, each of which was maintained in E. coli with a different copy number, and the best expression vector was selected. In addition, the S-adenosylmethionine biosynthesis pathway was engineered for optimal cofactor production. Through the combination of optimized POMT7 expression and cofactor production, the production of rhamnetin was increased up to 111 mg/l, which is approximately 2-fold higher compared with the E. coli strain containing only POMT7.

• The Journal of the Convergence on Culture Technology
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• 제8권1호
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• pp.409-415
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• 2022

In computer vision, an image of a measurement target is acquired using a camera. And feature values, vectors, and regions are detected by applying algorithms and library functions. The detected data is calculated and analyzed in various forms depending on the purpose of use. Computer vision is being used in various places, especially in the field of automatically recognizing automobile parts or measuring the quality. Computer vision is being used as the term machine vision in the industrial field, and it is connected with artificial intelligence to judge product quality or predict results. In this study, a vision system for judging the quality of automobile parts was built, and the results were compared by applying five machine learning classification models to the produced data.

• Journal of Plant Biotechnology
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• 제7권1호
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• pp.51-55
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• 2005

Many cloning vectors in which cDNAs can be inserted to the sense orientation have been developed. Uni-ZAP XR vector (Stratagene) should contain clones that are oriented to sense direction with respect to T3 RNA polymerase primer. Unexpectedly, large portions of cDNAs in Chinese cabbage cDNA library showed unusual insertions, antisense orientation and a hybrid of two different clones. Using two clones, 4H03 and 53-B10, derived from different cDNA libraries, we proposed and demonstrated the possibility of unusual-construct formation by in vitro translation and northern blot analysis. The 4H03 clone was inserted with inverse direction, and its transcript and translation product could be produced by T7 RNA polymerase, indicating that this clone is definitely inserted into inverse orientation. The 53-B10 that contains two independent genes was turned out to be a hybrid in which two genes are inserted to opposite direction each other. All unusual constructs might be due to the presence of small fragments of DNA, like adapter. However, the mechanism underlined the formation of unusual constructs is still remain to be solved.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 한국소음진동공학회 2010년도 추계학술대회 논문집
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• pp.543-543
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• 2010

The objective of this research is estimating the location of interested sound source by using the similarity between a beamformer output in time domain and the candidate signal. The waveform of beamformer output at the location of sound source is similar with the waveform emitted by that source. To estimate the location of sound source by using this feature, we define quantified similarity between candidate signal and beamformer output. The candidate signal describes the signal which is generated by interested source. In this paper, similarity is defined by four methods. The two methods use time vector comparison, and the other two methods use time-frequency map or linear prediction coefficients. To figure out the results and performance of localization by using similarities, we demonstrate two conditions. The one is when two pure tone sources exist and the other condition is when several bird sounds exist. As a consequence, inner product with two time-vectors and structural similarity with spectrograms can estimate the locations of interest sound source.

• Journal of the Korea Academia-Industrial cooperation Society
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• 제10권7호
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• pp.1702-1710
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• 2009

In this paper, we propose a problem space based search(PSBS) algorithm to solve parallel machine scheduling problem considering rework probabilities. For each pair of a machine and a job type, rework probability of each job on a machine can be known through historical data acquisition. Neighborhoods are generated by perturbing four problem data vectors (processing times, due dates, setup times, and rework probabilities) and evaluated through the efficient dispatching heuristic (EDDR). The proposed algorithm is measured by maximum lateness and the number of reworked jobs. We show that the PSBS algorithm is considerably improved from the result obtained by EDDR.

• Journal of IKEEE
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• 제5권2호
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• pp.211-220
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• 2001

This paper proposes a 2-D texture segmentation algorithm which is in close analogy to amplitude demodulation in communication systems. First, we show that it is theoretically possible to segment a multitexture image using an ideal filter followed by an amplitude demodulation block. However, in practice, the Gabor filter is used instead of the ideal filter because it has many desirable properties and especially it gives optimum space-bandwidth product. Our algorithm recovers all the texture regions containing the sinusoid with frequency to which the Gabor filter is tuned. We have demonstrated the discriminating power of our method in using a synthetic multitexture image. It is clear mathematically and easy to implement. Our method can be a good alternative to avoid many problems encountered in classifying the feature vectors in feature-based texture segmentation approaches.