• Natural Product Sciences
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• v.9 no.1
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• pp.44-48
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• 2003

The effect of aqueous extract of Stachys riederi var. japonica Miq. (Labiatae) (SRAE) on the immediate-type allergic reactions was investigated. SRAE was found to exhibit a inhibitory activity on the compound 48/80-induced systemic anaphylaxis in mice. SRAE inhibited the plasma histamine release induced by compound 48/80 in mice. In addition, SRAE also inhibited the passive cutaneous anaphylaxis reaction induced by IgE/anti-IgE in mice. The effect of SRAE on the histamine release from rat peritoneal mast cells (RPMC) was studied. SRAE inhibited the histamine release induced by compound 48/80 in RPMC. To clarify the mechanism of these inhibiting reactions, we investigated the effects of SRAE on cyclic AMP (cAMP). The level of cAMP in human leukemia cell line, HMC-1, when SRAE (1 mg/ml) was added, significantly increased compared with that of basal cells. These results indicate that SRAE may be beneficial in the treatment of immediate-type allergic reaction.

• Korean Journal Plant Pathology
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• v.13 no.4
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• pp.219-224
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• 1997

A PT-PCR (reverse transcription-polymerase chain reaction) diagnostic method for potato virus Y (PVY) was developed using primer pair derived from conserved region of coat protein genes of several PVY strains, A 764 bp PCR product was detected from several lines of potato cv. Atlantic. We could prove that the 764 bp DNA fragment was indeed the PVY gene by sequencing analysis. PVY detection method using RT-PCR technique was about tuber tissue.

• Natural Product Sciences
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• v.16 no.3
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• pp.185-191
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• 2010

Mast cell-mediated allergic disease is involved in many diseases such as anaphylaxis, asthma and atopic dermatitis. The discovery of drugs for the treatment of allergic disease is an important subject in human health. In the present study, the effect of water extract of Gleditsiae Spina (WGS) (Leguminosae), on compound 48/80-induced systemic allergic reaction, anti-DNP IgE antibody-induced local allergic reaction, and histamine release from human mast cell line (HMC-1) cells were studied. In addition, the effect of WGS on phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187 (A23187)-induced gene expression and secretion of pro-inflammatory cytokines were investigated using HMC-1 cells. WGS was anally administered to mice for high and fast absorption. WGS inhibited compound 48/80-induced systemic allergic reaction. WGS dose-dependently decreased the IgE-mediated passive cutaneous anaphylaxis. WGS reduced histamine release from HMC-1 cells. In addition, WGS decreased the gene expression and secretion of pro-inflammatory cytokines in PMA plus A23187-stimulated HMC-1 cells. These findings provide evidence that WGS could be a candidate as an antiallergic agent.

• Natural Product Sciences
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• v.19 no.1
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• pp.66-70
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• 2013

Ten compounds (1 - 10), palmitic acid (1), 10-nonacosanol (2), pentacosan-1-ol (3), phytol (4), ${\beta}$-sitosterol (5), ${\beta}$-sitosterol-3-O-${\beta}$-D-glucopyranoside (6), 2,4-dihydroxycinnamic acid (7), hyperoside (8), uridine (9) and adenosine (10), were isolated from the n-hexane and EtOAc-soluble fractions of the aerial parts of A. dioicus var. kamtschaticus (Rosaceae). The structures of these compounds were elucidated on the basis of spectroscopic evidence. All compounds (1 - 10) were isolated for the first time from this plant. Cytotoxicity of 1 - 10 against Jurkat T (T-lymphocytic leukemia cells), HeLa (Human cervical epitheloid carcinoma cells), MCF-7 (Human breast cancer cells), and HL-60 (Human promyelocytic leukemia cells) cell lines was measured. Compound 6 showed good cytotoxicity against HL-60 cell line with $IC_{50}$ value of 8.13 ${\mu}g/mL$. In addition, compounds 7 and 8 exhibited antioxidant activity with $IC_{50}$ values of 16.30 and 12.42 ${\mu}g/mL$, respectively.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2006.05a
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• pp.353-354
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• 2006

The automatic inspection system is developed for the formed end part of automotive fuel pipe. The developed system has functions of outer diameter and formed end length measurement by LVDT(linear variable differential transformer) together with burr cleaning of automotive fuel pipe. The measured data are managed and controlled in real time by embedded SPC(statistical process control) program. The system is composed of mechanical part, electronic part and developed software system. These three parts operate automatically by mutual communication with each other. The developed system showed good results in finding inferior goods and efficiency improvement of the fuel pipe production line. It also eliminated the unreliable manual inspection processes and improved the confidence of product quality.

• Journal of Fashion Business
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• v.1 no.4
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• pp.34-48
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• 1997

Dresses of the 1950s and 60s when the range and changes of fashion were wide and heavy were closely related with various styles of that time's architecture from the form, use of material, structure to the color. In this, study, characteristics of formalism and brutalism, main architectural streams of that period, such as line, structure, function, material, texture and color are analyzed in detail and these characteristics and those of dress are compared and analyzed to see if there are common aesthetic senses between them. It is particularly noteworthy that at that time formalistic architecture represented joy and abundance through enriched creation of appearance and flowing, symbolic linear beauty while the brutalistic produced young and vivid liveliness through functional, reasonable and moderate beauty, and this young and joyful atmosphere coincided exactly with frank, lively aesthetic senses of the young generation's fashion. Therefore, we can see common significance in the following fact ; architectural modes and fashion art of the 1950s and 60s were the result of more humane and enriched expressions from economical growth, and also product of that time's desire to adapt itself realistically to the rapid, complicated pop culture resulted from mass production.

• Journal of the Korean Society for Precision Engineering
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• v.23 no.5 s.182
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• pp.51-58
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• 2006

The necessities for heightening fuel efficiency as well as lightweight design, lead to an increase of the use of aluminum alloys in the automobile industry. Extruded aluminum profile channels are used widely for the design of frame parts as lightweight assemblies, especially if a high stiffness is needed. While many applications can be realized with forming of hollow square-sectioned extruded profiles such as a stretch bending and a hydro-forming, some applications demand the use of a press bending which can be hardly found in the previous study. In this study, by introducing the use of a press bending into car sub-frames, the demands for higher accuracy as well as higher flexible method than the conventional methods will be satisfied. With respect to the design of sub-frames, the process planning was performed from the shape of a sub-frame product. The designed processes were analyzed by the commercial FEM code, DEFORM-3D. Forming dies for the each process were designed and prototypes of sub-frames were manufactured by the verified farming process. In addition, some of the important features of design parameters in the press bending were reviewed.

• Natural Product Sciences
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• v.23 no.1
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• pp.1-4
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• 2017

Sinensetin, a pentamethoxyflavone, is known to exert various pharmacological activities including anti-angiogenesis, anti-diabetic and anti-inflammatory activities. However, its effects on the human mast cell - 1 (HMC-1) mediated inflammatory mechanism remain unknown. To explore the mediator and cellular inflammatory response of sinensetin, we examined its influence on phorbol 12-myristate 13-acetate (PMA) plus A23187 induced inflammatory mediator production in a human mast cell line. In this study, interleukin (IL)-6 production was measured using the enzyme-linked immunosorbent assay and reverse transcription polymerase chain reaction. Sinensetin inhibited PMA plus A23187 induced IL-6 production in a dose-dependent manner as well as IL-4, IL-5 and IL-8 mRNA expression. Furthermore, sinensetin inhibited signal transducer and activator of transcription 3 (STAT3) phosphorylation, suggesting that sinensetin inhibits the production of inflammatory mediators by blocking STAT3 phosphorylation. Moreover, sinensetin was found to inhibit nuclear factor kappa B activation. These findings suggest that sinensetin may be involved in the regulation of mast cell-mediated inflammatory responses.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.40 no.4
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• pp.66-73
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• 2008

When coated paperboard is printed, pressed into a groove with a creasing rule and folded, white line cracking occurs along the crease due to intensive mechanical pressure. The cracking will deteriorates product quality and waste resources. Effects of creasing pressure and ink dosage on the foldability of coated board were investigated. It was shown that applying an optimum pressure is important during creasing. When the pressure was too low, the crease formed was not sufficiently deep enough to enable precise folding. When an excess pressure was applied, fiber bonding was destroyed, resulted in unsatisfactory cracking. When the coated board was folded in machine direction (MD), long cracks were formed along MD. When it folded in cross direction (CD), the cracks were shorter and formed perpendicular to CD. Printing promoted cracking due to the decrease in flexibility of coated board. In addition, uneven ink film layer on the coating layer caused worse cracking.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.36 no.7
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• pp.813-822
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• 2012

Abnormal operating sounds radiated from a moving transfer robot in LCD (liquid crystal display) product lines have been used for the fault detection line of a robot instead of other source signals such as vibrations, acoustic emissions, and electrical signals. Its advantage as a source signal makes it possible to monitor the status of multiple faults by using only a microphone, despite a relatively low sensitivity. The wavelet packet transform for feature extraction and the artificial neural network for fault classification are employed. It can be observed that the abnormal operating sound is sufficiently useful as a source signal for the fault diagnosis of mechanical components as well as other source signals.