• Advances in concrete construction
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• v.15 no.2
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• pp.85-96
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• 2023

The characteristics of self-compacting concrete (SCC) made with fly ash and reinforced with polyester fibers were investigated in this research. Polyester fibers of 12 mm long and 15 micrometer diameters were utilized in M40 grade SCC mixtures at five different volume fractions 0.025%, 0.05%, 0.075%, 0.1%, 0.3% as a fiber reinforcement. To understand the influence of polyester fibers on passing ability, flowability, segregate resistance the J ring, L box, V funnel, slump flow and U box tests were performed. Polyester fibers have a direct influence, with a maximum of 0.075% polyester fibers producing excellent characteristics. ANN models were constructed using the testing data as inputs to anticipate the fresh and hardened characteristics as targeted outputs. The research revealed that R² values ranging from 0.900 to 0.997 appears to be a good correlation. The performance of ANN models and regression models for predicting the new characteristics of SCC is also evaluated.

• Microbiology and Biotechnology Letters
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• v.44 no.1
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• pp.68-73
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• 2016

Lactic acid bacteria showing both protease activity and the capacity to produce casein phosphopeptide (CPP) were isolated from Korean kimchi, a traditional food made from fermented vegetables. Among the 450 strains of isolated lactic acid bacteria, the strain MG-379 showed high protease activity and the highest ability to produce CPP. Characterization results showed that MG-379 was gram-positive and measured $0.6-0.8{\mu}m$ in diameter. DNA sequencing of MG-379 and comparison with other sequences using BLAST revealed a 100% identity with the sequence of Enterococcus faecalis. However, MG-379 showed a higher CPP-producing ability than E. faecalis KCCM 40450. Accordingly, MG-379 was newly named as E. faecalis MG-379. Amount of free calcium liberated by CPPs was 2227.5 and 1151.6 mg/kg for E. faecalis MG-379 and E. faecalis KCCM (control), respectively.

• Journal of the Korea Institute of Building Construction
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• v.3 no.4
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• pp.79-86
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• 2003

The purpose of study is to offer base data for high fluid concrete mix property, as grasp effect of aggregate to reach much more effect for producing high fluid concrete. For this study, there are three types of combined aggregates, river sand + river aggregate(type A), river sand + crusted aggregate(type B), washed sea sand + crushed aggregate(type C) and take a factor, water-contents, water-binder ratio and S/a. And so, we had following conclusion, resulting application-ability of high fluid mortar by K-slump tester to use a handy consistency measuring instrument. And so, we had following conclusion, resulting application-ability of high fluid concrete by K-slump tester to use a handy consistency measuring instrument. 1) In cafe of regular water binder ratio, high fluid concrete suffered much effect of combined aggregates and water binder ratio. Range of water binder ratio by combined aggregates is w/b 0.4 downward(type A and B), w/b 0.35 downward(type C). 2) Water contents to need for producing high fluid concrete is minimum 170kg/$\textrm{m}^3$ without regard to combined aggregates. 3) The effect of S/a on high fluid concrete by combined aggregates is approximately S/a 50% (type A and B), s/a 50-55% (type C). 4) Consistency measuring of high fluid concrete by K-slump tester is possible and first indication value, high fluid concrete can be produced, is 6~10.5cm.

• Journal of the Korean Society of Earth Science Education
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• v.12 no.2
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• pp.141-150
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• 2019

The purpose of this study is to investigate the effect of the pre-service teacher's maker project learning on self -directed learning ability and creative personality. This study established an eight-week period of experimental treatment from March to April 2019, and the students who participated in the study formed a research group of 75 students in three of their advanced classes who are attending the second semester of B University of Education and taking courses in 'Teaching Research 1'. The experimental treatment of research groups was conducted by the process of producing creative outputs through training of manufacturers. The experimental group of the study group was a process of producing creative output through maker education. The theme was set up to create creative output by group, worked with the main tool, 3D pen, and utilized the recycling material. When the creative output is completed, it is announced by presenting the actual product in each group. The results of the study are as follows. First, project learning by pre-service teachers had an effect on self-directed learning ability. Second, project learning by pre-service teachers had an effect on creative personality. Third, pre-service teachers was interested in learning about the project and responded positively to the students' perception.

• Journal of Labour Economics
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• v.40 no.4
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• pp.91-114
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• 2017

This paper shows that when children's ability is heterogeneous, a parent's choices about educational expenditures and fertility follow a pooling equilibrium or a separating equilibrium. Which of the two equilibria will prevail depends on the probability of getting a high-ability child as well as productivity differentials in producing children's adult human capital. Adopting the model of Acemoglu's (1999), this paper presents that the outcome of the pooling choice in the pooling regime and the outcome of the separating choice in the separating regime make the growth rate of human capital higher than otherwise. In addition, as the probability of a high-ability child increases, the growth rate of human capital in the separating equilibrium exceeds that in the pooling equilibrium.

• Journal of Microbiology and Biotechnology
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• v.6 no.5
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• pp.315-320
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• 1996

The role of glyoxylate bypass in a lysine-producing Corynebacterium lactofermentum strain was analyzed. Unlike the wild type, the strain expressed enzymes of glyoxylate bypass during growth in the fermentation broth containing glucose as the carbon source. To evaluate the importance of glyoxylate bypass in the strain, we disrupted chromosomal aceA by using a cloned fragment of the gene. Site-specific disruption of aceA which codes for the isocitrate lyase, the first enzyme of the bypass, was confirmed by Southern blot analysis. The aceA mutant strain completely lost isocitrate lyase activity and ability to grow in a minimal medium containing acetate as the sole carbon source. The mutant strain was similar to its parental strain in growth characteristics and produced comparable amounts of lysine in shake flasks containing glucose as the carbon source. The amount of oxaloacetate accumulated in the fermentation medium was similar for both strains, suggesting that expression of glyoxylate bypass does not necessarily lead to the increase in intracellular oxaloacetate. These data clearly demonstrate that glyoxylate bypass does not function as one of the routes of carbon supply for lysine production in the strain. It appears that the leakiness of the glyoxylate bypass in the strain might be the result of a secondary mutation which arose during previous strain development by random mutagenesis.

• Proceedings of the Materials Research Society of Korea Conference
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• 2009.11a
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• pp.14-14
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• 2009

High frequency electromagnetic(EM) waves are increasingly being applied in industries because of saturationat lower frequency bands as a result of huge demand. However, electromagneticinterference (EMI) has become a serious problem, and as a result, highfrequency EM absorbers are now being extensively studied. Also, recentdevelopments in absorber technology have focused on producing absorbers thatare thin, flexible, and strong. Hence, one-dimension ferrous nano-materials area potential research field, because of their interesting electronic andmagnetic properties. Commercially, EM wave absorbing products are made ofcomposites, which blend the insulating polymer with magnetic fillers. Inparticular, the shape of the magnetic fillers, such flaky, acicular, or fibrousmagnetic metal particles, rather than spherical, is essential for synthesizingthin and lightweight EM wave absorbers with higher permeability. High aspectratio materials exhibit a higher permeability value and therefore betterabsorption of the EM wave, because of electromagnetic anisotropy. Nanowires areusually fabricated by drawing, template synthesis, phase separation, selfassembly, and electrospinning with a thermal treatment and reduction process.Producing nanowires by the electrospinning method involves a conventionalsol-gel process that is simple, unique, and cost-effective. In thispresentation, Magnetic nanowire and dielectric materials coated magneticnanowire with a high aspect ratio were successfully synthesized by theelectrospinning process with heat treatment and reduction. In addition toestimating the EM wave absorption ability of the synthesized magnetic anddielectric materials coated magnetic nanowire with a network analyzer, weinvestigated the possibility of using these nanowires as high-frequency EM waveabsorbers. Furthermore, a wide variety of topics will be discussed such as thetransparent conducting nanowire and semiconducting nanowire/tube with theelectrospinning process.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.30-30
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• 2003

The present study examined the developmental ability of embryonic stem (ES) cells aggregated with mouse parthenogenetic embryos. Oocytes obtained from superovulated female mouse (BCF1) were treated with 7% ethanol and 5 $\mu\textrm{g}$/$m\ell$ cytochalasin B (CB) for producing pathenotes and in vitro fertilized with fresh sperm for producing normal embryos. The reporter vector (pNeoEGFP) were inserted into ES cells (129S4/svJae) by electroporation. At the 8-cell stage, in vitro fertilized embryos and pathenotes, which the zona pellucida was removed, were co-cultured with 5~10 ES cells for 4 hr. After in vitro fertilized embryos and parthenotes aggregated with ES cells were incubated to blastocyst stage, and these blastocysts transferred into the uterus of pseudopregnant recipients. The fertilized embryos aggregated with ES cells were successfully developed to offspring, but the parthenotes aggregated with ES cells failed to develop offsprings. However, genomic DNA of ES cells was detected in the pathenogenetic fetus by polymerase chain reactions at 15 day post gestation. In this study, results indicated that parthenotes aggregated with ES cells showed possible development to fetus. In the future, this method may help to produce transgenic chimera from parthenotes aggregated with ES cells.

• KSBB Journal
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• v.32 no.2
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• pp.90-95
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• 2017

Mammalian cell cultures have been used extensively to produce proteins for therapeutic agent because of their ability to perform post-translational modification including glycosylation. To produce recombinant protein, many factors and parameter are considered such as media composition, host cell type, and culture process. In this study, recombinant human erythropoietin (rhEPO) producing cell line was established by using glutamine synthetase system. To reduce serum concentration in media, we compared direct adaptation with step adaptation. Cell growth was faster in step adaptation. In low-level serum media, there were insufficient glucose for cell growth. Thus, we added glucose in low-level serum media from 2 g/L to 4.5 g/L. Titer of rhEPO was higher than other conditions at 4.5 g/L of glucose. Additionally, N-methyl-D-aspartate (NMDA), 13-cis-retinal, and pluronic F-68 (PF-68) were added to enhance productivity in CHO cell cultures. In conclusion, we applied CHO cell producing rhEPO to low-level of serum in media using step-adaptation. Also, we confirmed positive effect of NMDA, 13-cis-retinal, and PF-68.

• KSBB Journal
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• v.4 no.2
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• pp.162-166
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• 1989

When various strains of Streptomyces griseus and S. galbus were examined for the ability on the production of streptomycin in tryptic soy(TS) broth, S. griseus ATCC 27001 was found to be the best. S. griseus ATCC 12475 and ATCC 23345 showed also good growth and favorable production of streptomycin. Examination of various complex media reported in fermentation literatures for the industrial production of streptomycin indicated that glucose-soybean media-sodium chloride (GSS) broth and K (Chucken) broth gave higher yields of streptomycin than others studied by us. Examination of the ingredients of media producing streptomycin in high yield indicated that some components in soybean activated the production of streptomycin. Addition of meat extract enhanced the yield of streptomycin but it could be substituted with distillers solubles without much effect on the yield. Addition of corn steep liquor decreased the production of streptomycin.