• 한국엔터테인먼트산업학회논문지
• /
• 제15권5호
• /
• pp.19-31
• /
• 2021

본 연구의 목적은 국내 엔터테인먼트 기업의 CSR 활동, 리스크 관리, 프로듀싱 능력에 대한 소비자 평가가 통합 브랜드 자산 인식에 어떤 영향을 미치는지 살펴보는 것이다. 4년제 대학교에 재학 중인 대학생들을 대상으로 설문조사를 실시하였고, 총 423개의 데이터가 분석에 사용되었다. 연구결과, 첫째, 경제적 활동과 자선적 활동에 대한 소비자 평가가 통합 브랜드 자산 인식과 정적 인과관계를 나타냈다. 둘째, 국내 엔터테인먼트 기업에 대한 리스크 관리와 프로듀싱 능력 평가가 높을수록 통합 브랜드 자산 인식을 긍정적으로 하는 것으로 나타났다. 셋째, 4개 기업은 공통적으로 프로듀싱 능력의 주효과가 유의미하게 나타났다. 또한 CSR 활동과 리스크 관리에 대한 평가 수준에 관계없이 프로듀싱 능력 평가가 높은 집단은 낮은 집단에 비해 통합 브랜드 자산 인식이 높은 것으로 나타났다.

• 한국연초학회지
• /
• 제22권1호
• /
• pp.31-38
• /
• 2000

Seven flue-cured tobacco(Nicotiana tabacum) varieties and their 21 progenies in Fl and F2 generation, half diallel crosses(no reciprocal), were tested for the degree of heterosis and combining abilities in sucker producing character according to topping and suckering treatment. Significant difference in number and weight of sucker was observed among seven cultivars. The values of general combining ability(GCA) were greater than those of specific combining ability(SCA) in weight and number of sucker in F$_1$ generation. In topping and suckering plot, Cocker 86, McNair 944, and Va 115 revealed the significant negative GCA effects for number and weight of sucker, but By 104 and Speight G-28 did positive GCA. Without topping and suckering, GCA effects for sucker weight in Cocker 139 and Cocker 86 were negative and those for sucker number in Va. 115 were also negative. Negative SCA values in sucker weight could be observed in F$_1$ generation of Cocker 86 X BY 104 and Speiht G-28 X Cocker 86, in F$_2$ of Hicks $\times$ Speight G-28 and McNair 944 $\times$ BY 104 in topping and suckering plot. SCA in F$_1$ of McNair 944 X Cocker 86 and Speight G-28 X Cocker 139 was negative without topping and suckering. The value of heterosis in sucker producing character of F$_1$ hybrids was positive

• Journal of Microbiology and Biotechnology
• /
• 제20권7호
• /
• pp.1107-1113
• /
• 2010

Because conventional methods for detecting emetic-toxin-producing B. cereus are laborious and costly, various PCR assays, which are easy and cheap, have recently been reported. Therefore, this study estimated and compared the ability of various PCR assays to detect emetic-toxin-producing B. cereus strains isolated in Korea. The PCR assays were performed on 160 B. cereus strains, including 40 emetic-toxin-producing strains. Although the species-specific PCR assays were all shown to be highly specific, the sensitivities varied greatly. The accuracies of the primers were 97.5% (CER), 95.6% (EM1), 96.3% (RE234), 89.4% (CES), and 83.1% (Ces3R/CESR2). Moreover, the CER primer had a higher sensitivity (100%) than all the other primers tested, and a specificity of 96.7%. Thus, the CER primer was shown to be the most effective for screening the emetic-toxin-producing B. cereus strains tested in this study. However, the ability of these PCR assays to identify emetic-toxin-producing B. cereus should also be confirmed using other methods.

• 한국식품영양학회지
• /
• 제6권1호
• /
• pp.1-7
• /
• 1993

19 Samples of koji-starters using in brewing were collected from Korea and Japan, and then 31 strains of koji-molds were isolated from them. After Identification of the Isolate, rice koji was made with each strains, and its saccharogenic activity, dextrogenic activity, proteolytic activity, acid Producing ability, browning reaction and flavor were tested. Among 31 strains of isolates, 10 strains were Identified as Asp nwamori var. kawachii, 18 strains as Asp. oryzae, 3 strains as Asp. usamii mkt. shirousamii. The koji-starters made in Korea were composed of single species of koji-mold with same strain, but those made in Japan were composed of the mixture of different two species or the mixture of different 2 ∼4 strains in same species. Judging from amylolytic and proteolytic ability by species, Asp. awamori var. kawachii H1, I1 and 11, Asp. owsae J2, L2, M2, P3 and P4, and Asp. usamii writ. shirousamii S1 were better than the others. Mold strains isolated from Korean koji-starters were much lower in amylolytic or proteolytic activity than those from Japanese koji-starters. The typical characteristics for the 3 species of koji-molds were that Asp. awamori var. kawachii was strong in acid producing ability, but week in amylolytic and proteolytic ability, that Asp. owzae had strong amylolytic activity and good aroma, but produced little amount of acid, and that Asp. usamii mut. shirousamii had strong Proteolytic activity but some off-flavor.

• 대한소아치과학회지
• /
• 제30권4호
• /
• pp.660-666
• /
• 2003

치아우식증은 산에 의해 법랑질이 탈회되는 현상으로 소아 및 청소년기에 가장 흔히 발생하는 구강질환 중의 하나로서, 치아우식증의 원인요소 중 다른 인자들보다 객관적으로 조사할 수 있는 구강내 세균에 대한 연구가 활발히 이루어져 왔다. 이 연구는 구강내의 치태라는 고체상태의 조건과 유사한 상태로 Er:YAG 레이저가 구강내 산 생성세균인 S. mutans의 증식에 미치는 영향 및 레이저의 조사시간에 따른 차이를 평가하고자 하였다. Er:YAG 레이저의 구강내 산 생성 세균인 S. mutans에 대한 증식억제효과를 평가하기 위하여 S. mutans pellet에 Er:YAG 레이저를 비접촉식 방법으로, 50mJ, 10Hz, 그리고 조사시간을 1초, 3초, 5초, 7초, 9초로 달리하여 조사하고 이때의 세균의 성장률과 산 생성능을 0, 2, 4, 8, 12, 24, 36, 48시간 동안 측정한 결과 다음과 같은 결과를 얻었다. 1. 레이저를 조사한 군이 레이저를 조사하지 않은 대조군에 비해 세균의 성장률이 감소되었다(P<0.01). 2. 레이저를 조사한 후 12시간까지 1, 3, 5초 조사군에 비해 7, 9초 조사군에서 세균의 성장율이 유의하게 감소되었으나(P<0.05), 24시간 후부터는 서로 유의할만한 차이를 보이지 않았다. 3. 레이저를 조사한 시간이 증가함에 따라 세균의 산 생성능력이 회복되는 시간이 길어짐이 관찰되었고 24시간 후에는 대조군과 차이를 보이지 않음으로써 일정 시간 이후에는 점차적으로 산 생성능력이 회복됨을 알 수 있었다.

• Archives of Pharmacal Research
• /
• 제12권4호
• /
• pp.249-253
• /
• 1989

High producers or blocked mutants of aminoglycoside antibiotic-producing Streptomyces spp. were selected by application of an agar plug method and by culturing individual colonies in broth. The productivities of aminoglycoside antibiotic producing organisms were increased by selection of a high producer from colonies obtained by spreading spores of wild strain, or survived from treatment of a mutagen or from the colonies regenerated from protoplast-formation and cell-wall regenerations. Some mutagen treated colonies lost the ability to produce antibiotics (5-8%). Some A-factor negative and deostreptamine or streptidine negative mutants were obtained by N-methyl-N'-nitro-N-nitrosomethylguanidine (MNNG) treatment. Many of the survivors from the MNNG treatment lost the ability to produce antibiotics. Major colonies produced less amount of antibiotics ; only few survived colonies produced more antibiotics than the parent. Resistance of Streptomyces spp. against the antibiotics produced by itself was also markedly affected by mutagen treatment.

• Asian-Australasian Journal of Animal Sciences
• /
• 제15권7호
• /
• pp.1031-1035
• /
• 2002

This study was carried out to obtain conjugated linoleic acid (CLA) producing lactic acid bacteria for further study on the enzymes related to the production of CLA which has gained considerable attention and on the development as a probiotic culture. Total 34 lactic acid bacteria were isolated from 19 feces samples of healthy babies. CLA forming ability was measured spectrophotometrically by the modification of linoleate 12-cis, 11-trans-isomerase activity measuring method, and CLA of the cultures were extracted, methylated, and examined by HPLC analysis. CLA methyl ester of only one culture showing the highest value of CLA forming ability could be detected by HPLC analysis. The culture was found to be Gram positive, rods and catalase negative. It grows at $45^{\circ}C$ but not at $15^{\circ}C$, and was identified to be Lactobacillus fermentum on the basis of the biochemical characteristics and the utilization of substrates. These results provide an efficient experimental method to screen CLA producing lactic acid bacteria.

• Journal of Microbiology and Biotechnology
• /
• 제17권1호
• /
• pp.104-109
• /
• 2007

Lactic acid bacteria that accumulated ${\gamma}-aminobutyric$ acid (GABA) in culture medium were screened to identify strains with high GAB A-producing ability. One strain, MS, which was isolated from kimchi, showed the highest GABA-producing ability among the screened strains. MS was identified as Lactobacillus buchneri based on Gram-staining, metabolic characteristics, and 16S rDNA sequence determination, Optimum culture conditions for GABA production were determined: MRS broth containing 5% MSG, 1% NaCl, and 1% glucose, at an initial pH of 5.0, the incubation temperature at $30^{\circ}C$ for 36 h. Under these conditions, MS produced GABA at a concentration of 251 mM with a 94% GABA conversion rate. Moreover, culture extracts of Lb. buchneri MS partially or completely protected neuronal cells against neurotoxicantinduced cell death.

• Food Science and Biotechnology
• /
• 제15권6호
• /
• pp.896-901
• /
• 2006

Patulin is a food mycotoxin which induces genotoxicity and acute intestinal disease in infants. Patulin mainly originates from fruit putrefactive moulds, especially in apples, which necessitates the maintenance of strong safety standards against patulin for fresh and processed apples. To investigate the patulin producing moulds in Korean apples, 16 morphological types of fungi were isolated from Korean apples and a patulin producing fungus was identified based on a sequence analysis of the region of internal transcribed spacers (ITS5-5.8S-ITS4 region, 505 base pair) and the 26 rRNA D1/D2 region (527 base pair). Morphological analyses were also performed. The isolated patulin producing fungus was found to a representative species of Penicillium crustosum. The maximal patulin production ability of the isolated fungus (P. crustosum) and the patulin producing standard strain (P. griseofulvum, ATCC 46037) in an SY broth medium were 0.32 and 2.46 mg/L, respectively.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제8권2호
• /
• pp.83-88
• /
• 2003

A cellulose-producing strain isolated from rotten apples was identified as Gluconacetobacter hansenii based on its physiological properties and the 16S rDNA complete sequencing method, and specifically named Gluconacetobacter hansenii PJK. The amount of bacterial cellulose (BC) produced by G. hansenii PJK in a shaking incubator was 1.5 times higher than that produced in a static culture. The addition of ethanol to the medium during cultivation enhanced the productivity of bacterial cellulose, plus the supplementation of 1% ethanol into the culture medium made the produced BC aggregate into a big lump and thus protected the bacterial-cellulose-producing G. hansenii PJK cells in the shear stress field from being converted into non-cellulose-producing (Cel) mutants. Cells subcultured three times in a medium containing ethanol retained their ability to produce BC without any loss in the production yield.