Browse > Article
http://dx.doi.org/10.5713/ajas.2002.1031

Screening of Conjugated Linoleic Acid Producing Lactic Acid Bacteria from Fecal Samples of Healthy Babies  

Ham, J.S. (Products and Utilization Division, National Livestock Research Institute)
In, Y.M. (Products and Utilization Division, National Livestock Research Institute)
Jeong, S.G. (Products and Utilization Division, National Livestock Research Institute)
Kim, J.G. (Products and Utilization Division, National Livestock Research Institute)
Lee, E.H. (Products and Utilization Division, National Livestock Research Institute)
Kim, H.S. (Products and Utilization Division, National Livestock Research Institute)
Yoon, S.K. (Products and Utilization Division, National Livestock Research Institute)
Lee, B.H. (Department of Food Science, McGill University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.15, no.7, 2002 , pp. 1031-1035 More about this Journal
Abstract
This study was carried out to obtain conjugated linoleic acid (CLA) producing lactic acid bacteria for further study on the enzymes related to the production of CLA which has gained considerable attention and on the development as a probiotic culture. Total 34 lactic acid bacteria were isolated from 19 feces samples of healthy babies. CLA forming ability was measured spectrophotometrically by the modification of linoleate 12-cis, 11-trans-isomerase activity measuring method, and CLA of the cultures were extracted, methylated, and examined by HPLC analysis. CLA methyl ester of only one culture showing the highest value of CLA forming ability could be detected by HPLC analysis. The culture was found to be Gram positive, rods and catalase negative. It grows at $45^{\circ}C$ but not at $15^{\circ}C$, and was identified to be Lactobacillus fermentum on the basis of the biochemical characteristics and the utilization of substrates. These results provide an efficient experimental method to screen CLA producing lactic acid bacteria.
Keywords
Conjugated Linoleic; Acid; Lactic Acid Bacteria; Lactobacillus Fermentum;
Citations & Related Records

Times Cited By Web Of Science : 4  (Related Records In Web of Science)
Times Cited By SCOPUS : 3
연도 인용수 순위
1 Chin, S. F., W. Liu, J. M. Storkson, Y. L. Ha and M. W. Pariza.1992. Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens. J. of Food Comp. And Analysis 5:185-197.   DOI
2 Fogerty, A. C., G. L. Ford and D. Svoronos. 1988. Octadeca-9,11-dienoic acid in food stuffs and in the lipids of human blood and breast milk. Nutr. Rep. Int. 38:937-944.
3 Nichols, P. L., Jr., S. F. Herb and R. W. Riemenschneider. 1951. Isomers of conjugated linoleic acid. I. Alkali isomerized linoleic acid. J. Am. Chem. Soc. 73:247-252.   DOI
4 Pariza, M. W. and X. Y. Yang. 2000. Method of producing conjugated fatty acids. US Patent 6,060,304.
5 Brassart, D. and E. J. Schiffrin. 2000. Pre- and probiotics. In:Essentials of Functional Foods (Ed. M. K. Schmidl and T. P.Labuza). Aspen publishers, Inc. Gaithersburg, Maryland. pp.205-216.
6 De Man, J. C., M. Rogosa and M. E. Sharpe. 1960. A medium for the cultivation of lactobacilli. J. Appl. Bacteriol. 23:130-135
7 Chin, S. F., J. M. Storkson, W. Liu, K. J. Albright and M. W. Pariza. 1994. Conjugated linoleic acid (9,11- and 10,12-octadecadienoic acid) is produced in conventional but not germ-free rats fed linoleic acid. J. Nutr. 124:694-701.
8 Kepler, C. R. and S. B. Tove. 1969. Linoleate cis-12, trans-11 isomerase. In: Methods in Enzymology (Ed. J. M. Lowenstein). Vol. XIV. Academic Press, New York. pp. 105-110.   DOI
9 Parodi, P. W. 1994. Conjugated linoleic acid-A anticarcinogenic fatty acid present in milk fat. Aust. J. Dairy Technol. 49(2):93-97.
10 Sehat, N., M. P. Yurawecz, J. A. G. Roach, M. M. Mossoba, J. K. G. Kramer and Y. Ku. 1998. Silver-ion high-performance liquid chromatographic separation and identification of conjugated linoleic acid isomers. Lipids 33:217-222.   DOI   ScienceOn
11 Ha, Y. L., N. K. Grimm and M. W. Pariza. 1987. Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid. Carcinogenesis 8:1881-1887.   DOI   ScienceOn
12 Huang, Y. C., L. O. Luedecke and T. D. Shultz. 1994. Effect of cheddar cheese consumption on plasma conjugated linoleic acid in men. Nutrition Res. 14(3):373-386.   DOI   ScienceOn
13 Jiang, J., L. Bjorck and R. Fonden. 1998. Production of conjugated linoleic acid by dairy starter cultures. J. Appl. Microbiol. 85:95-102.   DOI   ScienceOn
14 Pariza, M. W. and W. A. Hargraves. 1985. A beef-derived mutagenesis modulator inhibits initiation of mouse epidermal tumors by 7, 12-dimethylbenz[a]anthracene. Carcinogenesis 6:591-593.   DOI   ScienceOn
15 Ip, C., S. F. Chin, J. A. Scimeca and M. W. Pariza. 1991. Mammary cancer prevention by conjugated dienoic derivative of linoleic acid. Cancer Res. 51:6118-6124.
16 Ogawa, J., K. Matsumura, S. Kishino, Y. Omura and S. Shimizu. 2001. Conjugated linoleic acid accumulation via 10-hydroxy-12-octadecaenoic acid during microaerobic transformation of linoleic acid by Lactobacillus acidophilus. Appl. and Environ. Microbiol. 67(3):1246-1252.   DOI   ScienceOn
17 Yoon, C. S. 2001. Development and utilization of conjugated linoleic acid producing dairy starter cultures. In: Proceedings of the 28th Korean Society for Food Science of Animal Resources Symposium. pp. 89-97.