• 지역사회간호학회지
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• 제10권2호
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• pp.508-524
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• 1999

We would like to identify the current status and the problem of the material for sexual education in Korea by analyzing the contents and the subjects of the materials. The 274 materials were collected from 80 institutes from March 1999 to June 1999. The collected ones were analyzed according to title. year of production. producer. subjects of materials and contents. Also, the materials in the internet was searched. Result were summarized by the descriptive statistics with SPSS/PC as followings: Type of materials were video tape (155), books (70), projection slides (43) and CD-ROM (6); Number of production from each institute were 54 from Planned Parenthood Federation of Korea, 39 from Korea Broadcasting System. 23 from Seoul School Health Center. 22 from Seoul Education and Science Research Institute, 16 from Korea Sexual Violence Relief Center, 13 from Republic of Korean National Redcross. and 10 from Korea Educational Development Institute; Subjects of the materials were middle and high school children (55), teachers (35), primary schoolchildren (17) and adult (14), preschool children (5). One hundred forty eight materials did not indicate the specific subjects; Thirty institutes had been providing the information for sexual education and the counseling. According to the above results. we suggest the followings: The systematic development of materials for sexual education appropriate to the specific subjects should be done: Materials in the CD-ROM and internet homepage should be developed more and more; The evaluation tool should be developed and adapted to the materials for the recommendation of the good materials; Database of the sexual materials should be provided for further development, evaluation and utilization.

• 농촌계획
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• 제22권4호
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• pp.171-182
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• 2016

Since FAO introduced the Globally Important Agricultural Heritage Systems(GIAHS) in 2002, 36 sites of 15 countries so far have been listed on GIAHS. This study aims to find the important agricultural heritages of Korea and to prepare the methods for them to be selected as GIAHS. We have analyzed the proposals of the 36 GIAHS listed in order to study the characteristics of their components of the agricultural heritage which worked for being selected. To analyze the components of the agricultural heritage first, agricultural heritage was classified into 13 types and 42 components in light of GIAHS criteria. Then central themes were set to analyze the relevant contents in the proposals. They were, the type of GIAHS Site, significant agricultural landscape, the agriculture-forestry-fisheries-livestock linkage systems, the multi-layered, inter-cropping, circulation cultivation systems, the soil and water management systems, the conservation of agrobiological diversity and genetic resources, the history of the agricultural heritage, the succession of traditional farming techniques, the cultural diversity and so on. Most GIAHS are located in mountains, grasslands, rivers and coasts, desert than in plains, through which GIAHS assure us that it is the heritage of human challenge to overcome the harsh geographic environment and maintain a livelihood. In these sites the traditional farming techniques are carried on, such as mountain clearings, terraced rice paddies, and burn fields, and the unique irrigation systems and agricultural landscapes are well maintained, and the eco-friendly traditional farming techniques utilizing abundant forest resources and agriculture are well handed down. The origin or home of crop growing, a variety of genetic crop storage, the world's largest crop producer and preserving cultural diversity are also important factors for the selection.

• Journal of Microbiology and Biotechnology
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• 제16권1호
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• pp.77-83
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• 2006

A gene encoding a $\gamma-butyrolactone$ autoregulator receptor was cloned from Saccharopolyspora erythraea, and the biochemical characteristics, including the autoregulator specificity, were determined with the purified recombinant protein. Using primers designed for the conserved amino acid sequence of Streptomyces $\gamma-butyrolactone$ autoregulator receptors, a 120 bp S. erythraea DNA fragment was obtained by PCR. Southern and colony hybridization with the 120 bp fragment as a probe allowed to select a genomic clone of S. erythraea, pESG, harboring a 3.2 kb SacI fragment. Nucleotide sequencing analysis revealed a 615 bp open reading frame (ORF), showing moderate homology (identity, $31-34\%$; similarity, $45-47\%$) with the $\gamma-butyrolactone$ autoregulator receptors from Streptomyces sp., and this ORF was named seaR (Saccharopolyspora erythraea autoregulator receptor). The seaR/pET-3d plasmid was constructed to overexpress the recombinant SeaR protein (rSeaR) in Escherichia coli, and the rSeaR protein was purified to homogeneity by DEAE-Sephacel column chromatography, followed by DEAE-ion-exchange HPLC. The molecular mass of the purified rSeaR protein was 52 kDa by HPLC gel-filtration chromatography and 27 kDa by SDS-polyacrylamide gel electrophoresis, indicating that the rSeaR protein is present as a dimer. A binding assay with tritium-labeled autoregulators revealed that rSeaR has clear binding activity with a VB-C-type autoregulator as the most effective ligand, demonstrating for the first time that the erythromycin producer S. erythraea possesses a gene for the $\gamma-butyrolactone$autoregulator receptor.

• Journal of Microbiology and Biotechnology
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• 제16권12호
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• pp.1841-1848
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• 2006

Insights into gene expression have the potential for improvement of antibiotic yield and the development of robust production hosts for use in recombinant biomolecule production. $Cubicin^{TM}$ (daptomycin for injection) is a recently approved antibiotic active against many Gram(+) pathogens, including those resistant to methicillin, vancomycin, and fluoroquinolones. Daptomycin is produced as a secondary metabolite by Streptomyces roseosporus. A 128 kb region of DNA including the daptomycin biosynthetic gene cluster (dpt) has been cloned. and sequenced. Using a selected array of nucleic acid probes representing this region, we compared the expression levels of the dpt genes between S. roseosporus wild-type (WT) and derived S. roseosporus high-producer of daptomycin (HP). We observed that the majority of the biosynthetic genes were upregulated in HP compared with WT; a total of 12 genes, including those encoding daptomycin synthetase, showed consistently and significantly higher expression levels, at least 5-fold, in HP compared with WT. In contrast, some genes, flanking the dpt cluster, were expressed at higher levels in the WT strain. The expression of housekeeping genes such as S. roseosporus rpsL, rpsG, and 16S (positive controls) and presumptive intergenic regions in the dpt cluster (negative control) were identical in the two strains. In addition, we compared transcription during the early, mid-log, and early-stationary phases of growth in the HP strain. The same set of genes was upregulated and downregulated under all conditions examined; housekeeping genes showed no relative change in expression level over the periods of growth tested. Analyses of this type would be of value in studies of strain improvement and also for the identification of gene regulation processes that are important for secondary metabolite production.

• 한국콘텐츠학회논문지
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• 제17권3호
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• pp.474-485
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• 2017

국보 제1호인 숭례문은 2008년 2월 10일 화재로 일부가 소실되었으나 화재 이후 복구 작업을 통해 2013년 5월 4일 시민에게 공개되었다. 이로 인해 숭례문은 국가적으로 큰 이슈가 되어 언론의 관심을 받으며 동시에 많은 연구의 대상이 되었다. 본 연구는 문화재로서 숭례문을 키워드로 하여 2002년부터 2016년까지 신문 기사에 대한 빈도분석을 통해 숭례문 관련 어떤 키워드들이 자주 나타나고 있는지에 대해 파악하였다. 또한 추출된 숭례문 관련 키워드들간 연관관계 분석을 통해 키워드간 연결의 맥락을 파악하고 분석하였다. 다음으로 숭례문 화재 전후, 언론사별 주요 키워드 추출을 통해 공통점과 차이점을 보여줌으로써 관점의 다양성을 제공하였다. 본 연구를 통해 문화재로서 숭례문 관련 키워드는 화재 이후에 나타난 키워드가 전체 기사에서 고빈도어로 나타남을 알 수 있었고 몇 가지 키워드간 상관관계가 높게 나타났다. 또한 화재 전후 키워드에는 명확한 차이를 보이고 있었으며 언론사별 키워드에서 상위 키워드들은 명확한 차이는 보여주지 않았지만 차상위 키워드들은 차이가 발생하여 언론사별로 주로 다루어진 기사들의 내용은 차이가 있다는 것을 발견했다. 본 연구는 문화재로서 숭례문 관련 기사에 대해 텍스트마이닝 기법을 활용하여 다량의 데이터를 분석하였음에 의미가 있으며 정보생산자 및 정보소비자들에게 숭례문 관련 기사의 동향과 정보를 제공할 수 있을 것이라 기대한다.

• 한국농공학회논문집
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• 제49권5호
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• pp.67-77
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• 2007

The images of MODerate resolution Imaging Spectroradiometer (MODIS) that provide wider swath and shorter revisit frequency than Land Satellite (Landsat) and Satellite Pour I' Observation de la Terre (SPOT) has been used fer land cover classification with better spatial resolution than National Oceanic and Atmosphere Administration/Advanced Very High Resolution Radiometer (NOAA/AVHRR)'s images. Due to the advantages of MODIS, several researches have conducted, however the results for the land cover classification using MODIS images have less accuracy of classification in small areas because of low spatial resolution. In this study, uncertainty of paddy fields classification using MODIS images was conducted in the region of Gyeonggi-do and the relation between this uncertainty of estimating paddy fields and topographical factors was also explained. The accuracy of classified paddy fields was compared with the land cover map of Environmental Geographic Information System (EGIS) in 2001 classified using Landsat images. Uncertainty of paddy fields classification was analyzed about the elevation and slope from the 30m resolution Digital Elevation Model (DEM) provided in EGIS. As a result of paddy classification, user's accuracy was about 41.5% and producer's accuracy was 57.6%. About 59% extracted paddy fields represented over 50 uncertainty in one hundred scale and about 18% extracted paddy fields showed 100 uncertainty. It is considered that several land covers mixed in a MODIS pixel influenced on extracted results and most classified paddy fields were distributed through elevation I, II and slope A region.

• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.1054-1059
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• 2005

An extracellular protease was identified from Bacillus subtilis KCCM 10257 by N-terminal sequencing and mass spectral analysis. The molecular mass of the extracellular protease was estimated to be 28 kDa by SDS-PAGE. Sequencing of the N-terminal of the protease revealed the sequence of A(G,S,R)QXVPYG(A)V(P,L)SQ. The N-terminal sequence exhibited close similarity to the sequence of other proteases from Bacillus sp. A mass list of the monoisotopic peaks in the MALDI-TOF spectrum was searched after peptide fragmentation of the protease. Six peptide sequences exhibiting monoisotopic masses of 1,276.61, 1,513.67, 1,652.81, 1,661.83, 1,252.61, and 1,033.46 were observed from the fragmented protease. These monisotopic masses corresponded to the lytic enzyme L27 from Bacillus subtilis 168, and the Mowse score was found to be 75. A doubly charged Top product (MS) at a m/z of 517.3 exhibiting a molecular mass of 1034.6 was further analyzed by de novo sequencing using a PE Sciex QSTAR Hybrid Quadropole-TOF (MS/MS) mass spectrometer. MS/MS spectra of the Top product (MS) at a m/z of 517.3 obtained from the fragmented peptide mixture of protease with Q-star contained the b-ion series of 114.2, 171.2, 286.2, 357.2, 504.2, 667.4, 830.1, and 887.1 and y-ion series of 147.5, 204.2, 367.2, 530.3, 677.4, 748.4, 863.4, and 920.5. The sequence of analyzed peptide ion was identified as LGDAFYYG from the b- and y-ion series by de novo sequencing and corresponded to the results from the MALDI-TOF spectrum. From these results the extracellular protease from Bacillus subtilis KCCM 10257 was successfully identified with the lytic enzyme L27 from Bacillus subtilis 168.

• 한국콘텐츠학회논문지
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• 제13권5호
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• pp.90-102
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• 2013

본 연구에서는 전 세계적으로 주목받고 있는 K-POP 한류의 성공요인을 분석하였다. K-POP은 한류의 대중문화가 아시아를 넘어 세계적인 주목을 받고 있음을 보여주며 새로운 킬러 콘텐츠로서 급부상하고 있다. 본 연구에서는 K-POP의 글로벌 확산의 성공요인을 분석하고 특히, 소셜 미디어를 비롯해서 스마트 미디어 환경의 변화가 대중문화예술의 글로벌화에 미치는 영향력을 탐색하고 관련 요구조건을 분석하였다. 또한, 국내외 실감콘텐츠 관련 제작사례를 탐구하고 다양한 콘텐츠의 재매개를 통해 K-POP한류의 확산가능성을 분석 하였다. 따라서, 본 연구에서는 한류의 지속화 방안을 위하여 K-POP한류 확산을 위한 잠재적인 시장분석을 수행하고 스마트 미디어 기반 실감 콘텐츠의 생산자, 플랫폼사업자, 네트워크 사업자, 가전사 등 효율적인 생태계 구축과 비즈니스 모델 개발을 통해 지속가능한 K-POP한류의 가능성을 제시하였다.

• 유통과학연구
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• 제5권1호
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• pp.89-105
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• 2007

아파트시장의 환경변화와 소비자 특성의 변화로 인해 아파트 선택시 소비자의 구매의사결정도 과거와는 매우 다른 양상을 보이고 있다. 아파트 건설업체들은 고객만족도를 높이기 위해서는 주거환경을 이해하고, 차별화된 주거가치를 고객들에게 제공하여야 한다. 그러나 지금까지의 선행연구에서는 주거환경이 주거가치, 주거만족, 재구매의도간의 관계에 대한 통합적 연구가 거의 없었다. 따라서 본 연구에서는 부동산뿐만 아니라 마케팅 분야의 관련 선행연구들을 토대로 주거환경이 주거가치, 주거만족, 재구매의도에 어떠한 영향을 미치며, 이러한 주거가치와 주거만족은 재구매의도에 어떤 영향을 미치는지를 분석하여 향후 아파트 건설업체의 경쟁력 강화를 위한 유용한 전략적 시사점을 제공하고자 하였다.

• Journal of Microbiology and Biotechnology
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• 제16권6호
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• pp.870-879
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• 2006

The present study describes the formation of succinic acid by a nonvirulent, highly osmotolerant Klebsiella pneumoniae strain SAP (succinic acid producer), its profile of metabolites, and enzymes of the succinate production pathway. The strain produced succinate along with other metabolites such as lactate, acetate, and ethanol under aerobic as well as anaerobic growth conditions. The yield of succinate was higher in the presence of $MgCO_3$ under $N_2$ atmosphere as compared with that under $CO_2$ atmosphere. Analysis of intracellular metabolites showed the presence of a smaller PEP pool than that of pyruvate. Oxaloacetate, citrate, and $\alpha$-ketoglutarate pools were considerably larger than those of isocitrate and fumarate. In order to understand the synthesis of succinate, the enzymes involved in end-product formation were studied. Levels of phosphoenolpyruvate carboxykinase, fumarate reductase, pyruvate kinase, and acetate kinase were higher under anaerobic growth conditions. Based on the profiles of the metabolites and enzymes, it was concluded that the synthesis of succinate took place via oxaloacetate, malate, and fumarate in the strain under anaerobic growth conditions. The strain SAP showed potential for the bioconversion of fumarate to succinate under $N_2$ atmosphere in the presence of $MgCO_3$. At an initial fumarate concentration of 10 g/l, 7.1 g/l fumarate was converted to 7 g/l succinate with a molar conversion efficiency of 97.3%. The conversion efficiency and succinate yield were increased in the presence of glucose. Cells grown on fumarate contained an 18-fold higher fumarate reductase activity as compared with the activity obtained when grown on glucose.