• Research in Plant Disease
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• v.9 no.2
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• pp.72-78
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• 2003

Eight fungicides including prochloraz, tebuconazole, benomyl, mancozeb, thiophanate-methyl, azoxystrobin, and fluazinam were examined for their control effects on Fusarium bulb and root rot of oriental orchid, Cymbidium goeringii. Among the chemicals, prochloraz and tebuconazole were the most effective on suppression of the causal pathogen, F. oxysporum in vitro and on control of the disease in vivo. Prochloraz and tebuconazole inhibited mycelial growth of the fungi 95~100% at 10ppm a.i. and microconidial germination 75~100% at 100ppm a.i. Prochloraz and tebuconazole showed 80~92% and 84~88% protective control value on the disease, respectively. However, curative effects on infected orchid were relatively low. Other chemicals showed no or lower than 20% curative and 50% protective control value. Results indicated that prochloraz and tebuconazole can be used for the control of the Fusarium bulb and root rot of oriental orchids. However, the chemicals need to be applied prior to the disease development to achieve successful control efficacy.

• The Korean Journal of Mycology
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• v.19 no.1
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• pp.85-87
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• 1991

Prochloraz of fungicide was applied on Mycogone perniciosa causing wet bubble in cultivated mushroom, Agaricus bisporus. In vitro, Prochloraz was an excellent fungicide on two strains of Mycogone, tolerant and non-tolerant to Benomyl, respectively. At the low dosage, Prochloraz more inhibited mycelial growth of mushrooms than Benomyl. At the higher dosage, Benomyl more inhibited the mycelial growth than prochloraz. The higher yield of sporophore of the mushroom with low inferction rate was abtained from several trial of Prochloraz. Prochloroz was concluded to be effective fungicide on Mycogone perniciosa on Agaricus cultivation.

• The Korean Journal of Pesticide Science
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• v.11 no.4
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• pp.331-338
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• 2007

The major fungal diseases which effecting garlic storage are blue mold and dry rot, caused by Penicillium hirsutum and Fusarium oxysporum, respectively. In order to reduce the damage by the pathogenic fungi, here we report the effects of 11 fungicides tested to reduce spoilage during storage of garlics. In the in vitro antimicrobial activity test, the fungicides, diphenylamine, prochloraz and tebuconazole showed 0.3, 2.2, and 1.3 nun inhibition zone to F. oxysporium, and cyprodinil, diphenylamine, fenbuconazole, hexaconazole, penconazole, prochloraz, propiconazole, pyrimethanil and tebuconazole exhibited 0.2, 2.4, 0.8, 0.4, 1.2, 1.5, 1.2, 0.4 and 1.5 mm to P. hirsutum, respectively. To test the in vivo control effect, when the diphenylamine, prochloraz, and tebuconazole were treated by standard concentration, the fungal mycelium of F. oxysporium started to grow 5 days after inoculation, and 80, 63.3 and 83.3% of the inoculated cloves are infected 11 days after inoculation. When the tebuconazole were treated by standard concentration, the P. hirsutum was completely inhibited the growth of the fungi. In case of diphenylamine, penconazole and propiconazole treatment, the P. hirsutum was observed 7 days after inoculation and $20{\sim}23.3%$ of the cloves were infected 11 days after inoculation. When cyprodinil, prochloraz and pyrimethanil were treated, pathogens occurred 5 days after inoculation and $60{\sim}100%$ of the cloves infected 11 days after inoculation. Three fungicides such as diphenylamine, prochloraz and tebuconazole also suppressed remarkably the infection and growth of F. oxysporium and P. hirsutum on garlic when both of the pathogens are inoculated after the garlic cloves were dipped for 10 min in the suspension of each agrochemical. Overall, diphenylamine, prochloraz and tebuconazole showed effective control efficacy on dry rot and blue mold There was significant correlation between in vitro and in vivo assay in diphenylamine and prochloraz to F. oxysporum and cyprodinil, prochloraz and pyrimethanil to P. hirsutum.

• Research in Plant Disease
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• v.15 no.2
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• pp.94-100
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• 2009

These experiments were conducted to improve the effect of seed disinfection on rice seed severely infected Bakanae disease by seed soaking into mixed solution of prochloraz EC and fludioxonil FS. We investigated the effects of various concentrations of two fungicides mixed solution on spore germination and mycelial growth of Fusarium fujikuroi. Mycelial growth was inhibited 100% at $10{\mu}g$/ml of prochloraz and 33.3% at $80{\mu}g$/ml of fludioxonil. Spore germination was inhibited 81.4% at $40{\mu}g$/ml of prochloraz. Interestingly, mixed solution of $5{\mu}g$/ml or $10{\mu}g$/ml of each fungicide inhibitied 100% of mycelial growoth and 99.2% of spore germination, respectively. Severely infected rice seeds soaked into mixed solution composed of $125{\mu}l$/ml of prochloraz and $50{\mu}l$/ml of fludioxonil showed 2.1% of disease symptoms compared to 20.4% of prochloraz $125{\mu}l$/ml, but higher concentrations of prochloraz decreased the seedling stand rate. When the seed soaking time was longer and temperature was higher, control effect on Bakanae disease was improved, but seedling stand was lower about 80% over $35^{\circ}C$.

• Research in Plant Disease
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• v.14 no.3
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• pp.176-181
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• 2008

This experiment was conducted to find the causes of ineffective seed disinfection methods such as rice seeds soaking in hot water and prochloraz EC solution when the rice seeds were severely infected by Bakanae disease. In case of rice seeds collected from severely diseased field by Bakanae disease, the pathogen as the forms of spores and mycelium were infected in plumule and inner and outer integument of embryo, aleurone layer, and pericarp layer. When the rice seeds were soaked in hot water, the appearances of spores and hypha on the outer pericarp layer were severely disordered, however those of inner region of outer integument and aleurone layer were shown normal. The membrane of hypha on the outer pericarp layer was destroyed within 24 hours, while some spores were healthy and germinated 7 days after soaking, when the rice seeds soaked 24 hours in 125 ppm prochloraz solution at $30^{\circ}C$. These results indicated that the seed disinfection methods were ineffective on the Bakanae disease severely infected rice seed because the hot water did not transmit the pericarp layer of rice seed and also prochloraz solution did not effectively destroy the spore of pathogen.

• The Korean Journal of Mycology
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• v.43 no.2
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• pp.112-117
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• 2015

To investigate the changes of the resistance to prochloraz of Fusarium species causing bakanae disease, Fusarium isolates were collected from various regions in Korea, and pathogenicity tests were performed using rice seeds. Minimum inhibitory concentration (MIC) and effective concentration of 50% ($EC_{50}$) values of isolates were determined using the agar dilution method. High frequency distribution of MIC values of prochloraz against isolates collected in 2006~2007 and 2013~2014 years were $3.125{\sim}6.25{\mu}g/mL$ and $6.25{\sim}12.5{\mu}g/mL$, respectively. The mean $EC_{50}$ value of isolates increased from $0.3142{\mu}g/mL$ in 2006~2007 to $0.8124{\mu}g/mL$ in 2013~2014. Based on the $EC_{50}$ value of isolates collected in 2006~2007, the resistant baseline of prochloraz was determined as $0.6{\mu}g/mL$. Compared with the ratio of resistant isolates in 2006~2007, the ratio of resistant isolates in 2013~2014 increased from 6.5% to 41.6%.

• Analytical Science and Technology
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• v.15 no.1
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• pp.67-71
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• 2002

The residual analysis and half-life time of imidazole fungicide prochloraz in soils (silty clay) were investigated by gas chromatography equipped electron capture detector (GC-ECD). The soil samples were extracted acetone/hexane(1:1) solvent and analyzed after separated by $LC-NH_2$ Sep-Pak solid column. Linear sensitivity of standard calibration curve was Y = 268.8600X + 0.0664, $R^2=0.9998$ between 0.05~1.00 ng. The detection limit was 0.02 mg/L and the average recoveries were 94.5~97.3% from the standard additional experiments with 0.10 and 0.40 mg/L. The half-life time was 24.4 days in room laboratory and 7.6 days in the field test soil.

• Korean Journal Plant Pathology
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• v.12 no.2
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• pp.226-230
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• 1996

1994~1995년 사이에 대전, 공주, 논산, 부여와 김해 등지에서 채집한 병든 딸기, 토마토, 오이에서 총 1,504 균주의 잿빛곰팡이병균을 분리하여 10 $\mu\textrm{g}$/ml의 약제를 넣은 PDA 배지에서 배양하면서 약제반응을 조사한 결과, benomyl에 대하여는 1,147(76.3%)균주, procymidone에 대하여는 614(40.8%)균주, prochloraz에 대하여는 189(12.6%)균주가 저항성으로 나타났다. UV 처리 및 약제 배지에서의 누대배양을 통하여 prochloraz저항성 균주를 선발하였는데, 이들의 생육 억제 최소 농도(MIC)는 8 $\mu\textrm{g}$/ml 이상이었고, 감수성균의 MIC는 2 $\mu\textrm{g}$/ml 이하였다. 병원력, 포자 발아율, 균핵형성과 같은 적응력과 관련된 생물학적 특성 비교에서 저항성 균주들은 감수성 균주보다 열등하거나 비슷한 정도로 나타났다.

• The Korean Journal of Pesticide Science
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• v.13 no.3
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• pp.148-158
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• 2009

We tested and selected some agrochemicals reducing the occurrence of major pests and diseases during garlic storage. Tebuconazole, diphenylamine and prochloraz as fungicides and dimethate as a insecticide were sprayed or drenched before harvest. And the harvested garlic was dipped in each of the agrochemicals. The residues of pesticides in garlic bulbs treated were analyzed every month from harvesting time for 6 months. In case of Danyang garlic, which was treated with pesticides before and after harvesting, the residues of diphenylamine, tebuconazole, prochloraz, and dimethoate ranged from 0.008 to 0.28, from 0.03 to 0.32, from 0.02 to 0.12, and from 0.02 to 0.25 mg/kg, respectively. In case of Uiseong garlic, the residues of diphenylamine, tebuconazole, prochloraz and dimethoate ranged from 0.008 to 0.09, from 0.08 to 0.45, from 0.02 to 0.57, and from 0.04 to 0.38 mg/kg, respectively. And, in case of Namdo garlic, the residues of diphenylamine, tebuconazole, prochloraz, and dimethoate ranged from 0.008 to 0.52, from 0.07 to 1.67, from 0.02 to 0.17, and from 0.03 to 0.73 mg/kg, respectively. Some of the garlic samples treated with tebuconazole exceeded its maximum residue limits (MRLs) of 0.1 mg/kg set by Korea Food Drug Administration (KFDA), but dimethoate was detected below its MRL of 1.0 mg/kg. In case of diphenylamine and prochloraz, their MRLs for garlic were not set. Adapting their MRLs, 5.0 mg/kg of diphenylamine for apple and pear and 0.5 mg/kg of prochloraz for strawberry and grape, residue levels of diphenylamine and procloraz were below than their MRLs, with the exception of samples two times treated with procloraz in Namdo garlic. These results indicate that dimethoate can be used as an agrochemical to control the postharvest disease in garlic in only MRL aspect.

• Research in Plant Disease
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• v.20 no.1
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• pp.54-58
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• 2014

Anthracnose crown rot (ACR), caused by Colletotrichum fructicola, is a serious disease of strawberry in Korea. The primary inoculums of ACR were symptomless strawberry plants, plant debris, and other host plants. To effectively control anthracnose in symptomless transplanted strawberries, it is necessary to use disease-free plants, detect the disease early, and apply a fungicide. Therefore, in 2010 and 2011, we evaluated the efficacy of pre-plant fungicide dips by using strawberry transplants infected by C. fructicola for the control of anthracnose. Dipping plants in prochloraz-Mn for 10 min before planting was most effective for controlling anthracnose in symptomless strawberry plants and resulted in more than 76% control efficacy. Azoxystrobin showed a control efficacy of over 40%, but plants treated with pyraclostrobin, mancozeb and iminoctadine tris showed high disease severity. The control efficacy of the dip treatment with prochloraz-Mn did not differ with temperature and time. Treatment with prochloraz-Mn for more than an hour caused growth suppression in strawberry plants. Therefore, the development of anthracnose can be effectively reduced by dipping strawberry plants for 10 min in prochloraz-Mn before planting.