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http://dx.doi.org/10.4489/KJM.2015.43.2.112

Monitoring for the Resistance to Prochloraz of Fusarium Species Causing Bakanae Disease in Korea  

Choi, Hyo-Won (Crop Protection Division, National Academy of Agricultural Science)
Lee, Yong Hwan (Disaster Management Division, Rural Development Administration)
Hong, Sung Kee (Microbial Safety Team, National Academy of Agricultural Science)
Lee, Young Kee (Crop Protection Division, National Academy of Agricultural Science)
Nam, Young Ju (Crop Protection Division, National Academy of Agricultural Science)
Lee, Jae Guem (Crop Protection Division, National Academy of Agricultural Science)
Han, Song Hee (Crop Protection Division, National Academy of Agricultural Science)
Publication Information
The Korean Journal of Mycology / v.43, no.2, 2015 , pp. 112-117 More about this Journal
Abstract
To investigate the changes of the resistance to prochloraz of Fusarium species causing bakanae disease, Fusarium isolates were collected from various regions in Korea, and pathogenicity tests were performed using rice seeds. Minimum inhibitory concentration (MIC) and effective concentration of 50% ($EC_{50}$) values of isolates were determined using the agar dilution method. High frequency distribution of MIC values of prochloraz against isolates collected in 2006~2007 and 2013~2014 years were $3.125{\sim}6.25{\mu}g/mL$ and $6.25{\sim}12.5{\mu}g/mL$, respectively. The mean $EC_{50}$ value of isolates increased from $0.3142{\mu}g/mL$ in 2006~2007 to $0.8124{\mu}g/mL$ in 2013~2014. Based on the $EC_{50}$ value of isolates collected in 2006~2007, the resistant baseline of prochloraz was determined as $0.6{\mu}g/mL$. Compared with the ratio of resistant isolates in 2006~2007, the ratio of resistant isolates in 2013~2014 increased from 6.5% to 41.6%.
Keywords
Bakanae disease; Fusarium fujikuroi; Prochloraz resistance;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
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1 Kim CK. Ecological studies of bakanae disease of rice, caused by Gibberella fujikuroi. Kor J Plant Prot 1981;20:146-50.
2 Sung JM, Yang SS, Lee EJ. Pathogenicity in nursery box and symptom appearance and yield damage in paddy field by each strain of Fusarium moniliforme. Kor J Mycol 1984; 12:65-73
3 Park HG, Shin HR, Lee Y, Kim SW, Kwon OD, Park IJ, Kuk YI. Influence of water temperature, soaking period, and chemical dosage on bakanae disease of rice (Gibberella fujikuroi) in seed disinfection. Kor J Pestic Sci 2003;7:216-22.
4 Park WS, Choi HW, Han SS, Shin DB, Shim HK, Jung ES, Lee SW, Lim CK, Lee YH. Control of bakanae disease of rice by seed soaking into the mixed solution of prochloraz and fludioxonil. Res Plant Dis 2009;15:94-100.   DOI   ScienceOn
5 Shin MU, Kang HJ, Lee YH, Kim HT. Detection for the resistance of Fusarium spp. isolated from rice seeds to prochloraz and cross-resistance to other fungicides inhibiting sterol biosynthesis. Kor J Pestic Sci 2008;12:277-82.
6 Lee YH, Kim SY, Choi HW, Lee MJ, Ra DS, Kim IS, Park JW, Lee SW. Fungicide resistance of Fusarium fujikuroi isolates isolated in Korea. Kor J Pestic Sci 2010;14:427-32.
7 Dyer PS, Hansen J, Delaney A, Lucas JA. Genetic control of resistance to the sterol $14\alpha$-demethylase inhibitor fungicide prochloraz in the cereal eyespot pathogen Tapesia yallundae. Appl Environ Microbiol 2000;66:4599-604.   DOI
8 Dijkhuizen JP, Ogawa JM, Manji BT. Activity of captan and prochloraz on benomyl-sensitive and benomyl-resistant isolates of Monilinia fructicola. Plant Dis 1983;67:407-9.   DOI
9 Decognet V, Cereeau V, Jouan B. Control of Phoma exigua var. linicola on flax by seed and foliar spray treatments with fungicides. Crop Prot 1994;13:105-8.   DOI   ScienceOn
10 Leroux P, Gredt M. Evolution of fungicide resistance in the cereal eyespot fungi Tapesia yallundae and Tapesia acuformis in France. Pestic Sci 1997;51:321-7.   DOI
11 Kurt S, Dervis S, Sahinler S. Sensitivity of Verticillium dahliea to prochloraz and prochloraz-manganese complex and control of Verticillium wilt of cotton in the field. Crop Prot 2003;22: 51-5.   DOI   ScienceOn
12 Yang HF, Ji MX, Yao KB, Shu ZL. Study on resistance of Fusarium monilifore to prochloraz and its management. Acta Agriculturae Jiangxi 2013;25:94-6.
13 Ogawa K, Takeda S. Population of benomyl-resistant rice bakanae fungus in paddy field. Ann Phytopathological Soc Jpn 1990;56:247-9.   DOI
14 Amatulli MT, Spadaro D, Gullino ML, Garibaldi A. Molecular identification of Fusarium spp. associated with bakanae disease of rice in Italy and assessment of their pathogenicity. Plant Pathol 2010;59:839-44.   DOI   ScienceOn
15 Wulff EG, Sorensen JL, Lübeck M, Nielsen KF, Thrane U, Torp J. Fusarium spp. associated with rice Bakanae: ecology, genetic diversity, pathogenicity and toxigenicity. Environ Microbiol 2010;12:649-57.   DOI   ScienceOn
16 Jahan Quazi SA, Meon S, Jaafar H, Ahmad ZA. Characterization of Fusarium proliferatum through species specific primers and its virulence on rice seeds. Int J Agric Biol 2013;15: 649-56.
17 Cowger C, Mundt CC. Aggressiveness of Mycosphaerella graminicola isolates from susceptible and partially resistant wheat cultivars. Phytopathology 2002;92:624-30.   DOI   ScienceOn
18 Mohd Zainudin NA, Razak AA, Salleh B. Bakanae disease of rice in Malaysia and Indonesia: etiology of the causal agent based on morphological, physiological and pathogenicity characteristics. J Plant Prot Res 2008;48:475-85.
19 Yang L, Gao F, Shang L, Zhan J, McDonald BA. Association between virulence and triazole tolerance in the phytopathogenic fungus Mycosphaerella graminicola. PLoS One 2013;8: e59568.   DOI