• Title/Summary/Keyword: Process Safety

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Development of multiplex PCR-based detection method for five approved LM canola events in Korea (Multiplex PCR 방법을 이용한 국내 승인 5개 LM 유채의 검출법 개발)

  • Jo, Beom-Ho;Lee, Jung Ro;Choi, Wonkyun;Moon, Jeong Chan;Shin, Su Young;Eum, Soon-Jae;Seol, Min-A;Kim, Il Ryong;Song, Hae-Ryong
    • Journal of Plant Biotechnology
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    • v.42 no.2
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    • pp.117-122
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    • 2015
  • Canola is a crop globally used for production of oil and biofuel. Cultivation area and import volume of living modified (LM) canola have been increasing every year. As canola import dependence has reached 100% in Korea, efforts have been made for safety management of LM canola and ecological risk assessment. We developed a set of multiplex PCR method for simultaneous detection of 5 LM canola events (Topas 19/2, Rf3, Ms8, RT73 and T45) approved in Korea. The multiplex PCR assay developed allows amplification of estimated products of 5 LM canolas from event specific primer sets. Primer extension time was skipped for a time-consuming process and two annealing steps (20 cycles at $55^{\circ}C$ and 20 cycles at $60^{\circ}C$) were performed for yielding the best result which was sufficient to distinguish five LM canolas. Our results suggest that multiplex PCR method provides a cost and time-effective approach for LM canola detection.

A Study on the Use of Drones for Disaster Damage Investigation in Mountainous Terrain (산악지형에서의 재난피해조사를 위한 드론 맵핑 활용방안 연구)

  • Shin, Dongyoon;Kim, Dajinsol;Kim, Seongsam;Han, Youkyung;Nho, Hyunju
    • Korean Journal of Remote Sensing
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    • v.36 no.5_4
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    • pp.1209-1220
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    • 2020
  • In the case of forest areas, the installation of ground control points (GCPs) and the selection of terrain features, which are one of the unmanned aerial photogrammetry work process, are limited compared to urban areas, and safety problems arise due to non-visible flight due to high forest. To compensate for this problem, the drone equipped with a real time kinematic (RTK) sensor that corrects the position of the drone in real time, and a 3D flight method that fly based on terrain information are being developed. This study suggests to present a method for investigating damage using drones in forest areas. Position accuracy evaluation was performed for three methods: 1) drone mapping through GCP measurement (normal mapping), 2) drone mapping based on topographic data (3D flight mapping), 3) drone mapping using RTK drone (RTK mapping), and all showed an accuracy within 2 cm in the horizontal and within 13 cm in the vertical position. After evaluating the position accuracy, the volume of the landslide area was calculated and the volume values were compared, and all showed similar values. Through this study, the possibility of utilizing 3D flight mapping and RTK mapping in forest areas was confirmed. In the future, it is expected that more effective damage investigations can be conducted if the three methods are appropriately used according to the conditions of area of the disaster.

Characteristic of Local Behavior in Orthotropic Steel Deck Bridge with Open Ribs according to Running Vehicle (주행차량에 따른 개단면 강바닥판 교량의 국부거동 특성)

  • Lee, Sung-Jin;Kyung, Kab-Soo;Park, Jin-Eun;Lee, Hee-Hyun
    • Journal of Korean Society of Steel Construction
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    • v.24 no.1
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    • pp.101-108
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    • 2012
  • The orthotropic steel deck bridge made by using relatively thin steel plate, and structural members such as transverse and longitudinal ribs, cross beam, etc. in the bridge are fabricated with complex shape by welding. Therefore, the possibility occurring deformation and defects by welding is very high, and stress states in the welded connection parts are very complex. Also, the fatigue cracks in orthotropic steel deck bridge are happening fromthe welded connection parts of secondary member than main member. However, stress evaluation for main members is mainly carried out in the design process of the bridge, detailed stress evaluation and characteristic analysis is not almost reviewed in the structural details which fatigue crack occurred. For the orthotropic steel deck bridge with open ribs which has been serviced for 29 years, in this study, the cause of fatigue crack is investigated and the fatigue safety of the bridge is examined based on fieldmeasurement by the loading test and real traffic condition. Also, structural analyses using gridmodel and detailed analysis model were carried out for the welded connection parts of longitudinal rib and diaphramthat fatigue crack occurred. Additionally, the behavior characteristics due to running vehicles were investigated by using influence area analysis for these structural details, and the occurrence causes of fatigue crack in the target bridge were clarified.

Development of Evaluation Factors for Selecting Operator of Rail Traffic-Control (철도 관제권 운영주체 선정을 위한 평가항목 개발)

  • Oh, Jae Kyoung;Chung, Sung Bong;Kim, Ji Yeon;Kim, Sigon
    • Journal of Korean Society of Transportation
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    • v.32 no.4
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    • pp.327-335
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    • 2014
  • The Korean government, as of last year, is attempting to introduce a competitive system in the rail-market. However there are some pertinent issues which need to be addressed in order to select the best possible organization for optimum railway traffic control. As there are no standard guidelines in selecting an agency, objective evaluation factors need to be properly applied to ensure the best possible decision is made. Through literature review and various interviews with experts, appropriate criteria were selected to evaluate the suitability of each potential operator. As generally mentioned, both safety and efficiency play vital roles in the selection process but in addition, there are other factors such as security and fairness, which also need to be considered. The individual weight of these factors were calculated by the AHP, in which three groups of experts were interviewed to acquire their expertise. According to the results, as a potential operator, the score of a railway operating company and the management cooperation of railway is 2.75 and 3.85, respectively. In the future, in preparation towards becoming a competitive rail-market, this research is expected to assist in choosing the best party to control the rail traffic system.

Tracking the History of the Three-story Stone Pagoda from the Goseonsa Temple Site in Gyeongju throughan Analysis of Component (부재 해석을 통한 경주 고선사지 삼층석탑의 연혁 추적)

  • Jeon, Hyo Soo
    • Conservation Science in Museum
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    • v.21
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    • pp.41-52
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    • 2019
  • The findings of a 2017 safety inspection of the Three-story Pagoda from the Goseonsa Temple site in Gyeongju suggested the possibility that the stone for the second story of the pagoda may have been rotated after the pagoda was disassembled for removal from its original site in 1975. The materials from the pagoda were investigated using photographs and other relevant data from both the Japanese colonial period and from around 1975. The analysis found that the materials of the pagoda were not changed after analleged reconstruction in 1943, but that during the process of relocating the pagoda in 1975 the body of the second story was indeed rotated counter clockwise by 90 degrees and one of the four stone elements making up the first-story roof was exchanged with a part from the second-story roof. In order to discover whether the materials had been incorrectly placed, each part of the pagoda was precisely measured and the elements of the roofs were virtually reconstructed using 3D scanning data. The investigation did not find any singularities with in the components of each roof; the four part sof the first-story roof were 75 to 76 centimeters thick and those for the second-story roof were 78 to 79 centimeters thick. The connections between each part of the roofs also appeared natural. This seems to indicate that there was indeed an undocumented repair of the pagoda at some point between its creation and 1943 and an error that took place during this repair was corrected in 1975. In addition, the study suggested a possibility that the body of the second story was rotated counter clockwised to a change in the locations of parts of the two roofs.

Establishment of $F_0$-value Criterion for Canned Tuna in Cottonseed Oil (참치 기름담금 통조림의 $F_0$-값 설정에 관한 연구)

  • HAN Bong-Ho;CHO Hyun-Duck;YU Hong-Sik;KIM Sang-Ho;CHUNG Youn-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.27 no.6
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    • pp.675-681
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    • 1994
  • [ $F_0$ ]-values of the canned tuna in cottonseed oil (CTCO) were investigated under different sterilizing conditions to optimize the energy consumption and microbiological safety. The $F_0$-values were measured using a microcomputer based technique. The exact cold point was not the volumetric center of the cans, and it was located in the center of meat mass in can which had ca. $6\%$ of head space. Location of the test cans in retort showed no remarkable influence on the $F_0$-values when the cans were jumble loaded. The process time before sterilization should be shortened as much as possible to prevent the contamination of microorganisms. Thermophilic spore forming bacteria found from raw and precooked tuna were Bacillus subtilis, Bacillus cereus and Bacillus pasteurii, and the most heat resistant was Bacillus subtilis. The rational $F_0$-value for the CTCO obtained from the preservation test was regarded as 6min.

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The Analysis of Elementary Pre-service Teachers' Reflective Thinking and Experiment Performance Ability on Photosynthesis Experiment (광합성 실험에서 나타난 초등 예비교사들의 반성적 사고와 실험 수행 능력 분석)

  • Kim, Dong-Ryeul
    • Journal of Korean Elementary Science Education
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    • v.34 no.4
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    • pp.502-518
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    • 2015
  • In order to find out Elementary pre-service teachers' reflective thinking and experiment performance ability related with Photosynthesis Experiment in the Korea Elementary School Science Textbook, the research is conducted targeting Elementary pre-service teachers. They are asked to carry out the experiment and write their own report about the difficulties and solutions of exploration process. This study aims to analyze Elementary pre-service teachers' reflection and experiment performance ability on Photosynthesis experiment based on 10 groups' reports and presentation materials. Reflective thinking extracts 108 statements which is associated with the four types of the sentence 'Knowledge, Procedure, Orientation, Attitude' in 10 reports. There are many sentences about reflective thinking acquired through analysis of the photosynthesis experiment. reflective thinking about the newly discovered type or changed concepts through experimentation in Knowledge is at the highest frequency. 56 sentences in relation to the ability to perform experiments are extracted by adding 4 different types of reflective thinking in 10 groups shown the highest frequency group and the lowest frequency group's report through analyzing 4 steps 'Experimental preparation and safety accident prevention', 'Experiments performance', 'Experimental results and generalization', and 'Experimental results and feedback.' Results of the analysis showed that there are the biggest difference between the two groups in 'experiment results supplement and feedback step.' In the lowest group's report, there's no contents related with 'Computer-assisted information processing' in the 'Experimental results summary and generalization stage', 'Alternative reagents and materials research', and 'Devising alternative experiment methods'.

A study on the vulnerability of integrity verification functions of android-based smartphone banking applications (안드로이드 스마트폰 뱅킹 앱 무결성 검증 기능의 취약점 연구)

  • Kim, Soonil;Kim, Sunghoon;Lee, Dong Hoon
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.23 no.4
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    • pp.743-755
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    • 2013
  • In recent years, the malicious apps with malicious code in normal apps are increasingly redistributed in Android market, which may incur various problems such as the leakage of authentication information and transaction information and fraudulent transactions when banking apps to process the financial transactions are exposed to such attacks. Thus the financial authorities established the laws and regulations as an countermeasures against those problems and domestic banks provide the integrity verification functions in their banking apps, yet its reliability has not been verified because the studies of the safety of the corresponding functions have seldom been conducted. Thus this study suggests the vulnerabilities of the integrity verification functions of banking apps by using Android reverse engineering analysis techniques. In case the suggested vulnerabilities are exploited, the integrity verification functions of banking apps are likely to be bypassed, which will facilitate malicious code inserting attacks through repackaging and its risk is very high as proved in a test of this study. Furthermore this study suggests the specific solutions to those vulnerabilities, which will contribute to improving the security level of smartphone financial transaction environment against the application forgery attacks.

A Study on Microbiological Critical Limit in Sterilization processing of Fried Kimchi Soup (볶음김치스프 제조공정중 살균공정에 대한 미생물학적 한계기준에 관한 연구)

  • Kwon, Sang-Chul
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.13 no.9
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    • pp.4018-4024
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    • 2012
  • The purpose of this study was to application in the HACCP(Hazard Analysis Critical control) system of fried kimchi soup. The establishment of Critical limit during sterilization processing was measured by sterilization temperature, sterilization time, sensory test, storage test and pH change in storage for 30 days (May 1~30, 2012). Before sterilization, general bacteria, coliform and thermophile bacteria were detected to be $6.00{\times}10^5\;CFU/m{\ell}$, $7.50{\times}10^2\;CFU/m{\ell}$ and $2.75{\times}10^2\;CFU/m{\ell}$, respectively. In contrast, all microbial was not detected after sterilization($90{\pm}5^{\circ}C$, $22{\pm}5$ mins). The sensory test was decided as the most delicious kimchi according to $90{\pm}5^{\circ}C$, $22{\pm}5min$. In conclusion, the sterilization process of fried kimchi soup would be a great alternative to prevention, decreasing and removing of harmful microorganism, such as general bacteria, coliform and thermoduric bacteria etc. Therefore, the critical limit of sterilization temperature and time for quality control and biosafety was established at $90{\pm}5^{\circ}C$, $22{\pm}5$ mins. And it suggested that HACCP plan was necessary for monitoring method, monitoring cycle, problem solving method, education, training and record management during sterilization processing.

Concepts of Disaster Prevention Design for Safety in the Future Society

  • Noh, Hwang-Woo;Kitagawa, Keiko;Oh, Yong-Sun
    • International Journal of Contents
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    • v.10 no.1
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    • pp.54-61
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    • 2014
  • In this paper, we propose a pioneering concept of DPD(Disaster Prevention Design) to realize a securable society in the future. Features of danger in the future society are expected to be diverse, abrupt occurring, large scale, and complicated ways. Due to increment of dangers with their features of uncertainty, interactivity, complexity, and accumulation, human-oriented design concept naturally participates in activities to prevent our society against disasters effectively. We presented DPD is an essential design activity in order to cope with dangers expected in the future societies as well as realize securable environments. DPD is also an integrated design aids including preemptive protections, rapid preparing, recovery, and interactive cooperation. We also expect these activities of DPD is effective for generation of new values in the market, satisfaction of social needs, expansion of design industry, and a novel chance for development in the future society. Throughout this paper, we submit various aspects of DPD concepts including definition, classification, scope, necessity, strategy, influencing elements, process, and its principle. We expect these concepts will be the seed and/or basement of DPD research for the future works. For the direction of study for DPD in the future, we emphasize alarm system for preemptive protection rather than recovery strategy for the damage occurred. We also need to research about progressive prevention techniques and convergence with other areas of design. In order to transfer the concept of product design from facility-oriented mechanism to human-oriented one, we should develop new kinds of city basis facilities, public-sense design concepts referred to social weak-party, e-Learning content design preparing disasters, and virtual simulation design etc. On the other hand, we have to establish laws and regulations to force central and/or provincial governments to have these DPD strategies applying their regional properties. Modern design activities are expanding to UI(user interface) content design area overcoming the conventional design concept of product and/or service. In addition, designers are recognized as art directors or life stylists who will change the human life and create the social value. DPD can be divided into prevention design, preparedness design, response design, and recovery design. Five strategies for successful DPD are Precaution-oriented, Human-oriented, Sense-oriented, Legislation, and Environment Friendly Strategies.