• Korean Journal of Microbiology
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• v.18 no.3
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• pp.133-147
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• 1980

Washed mycelia of Aspergillus nidulans FGSC159 were incubated in CMC minimal liquid medium and the culture filtrate which contained induced extracellular cellulase was fractionated by a three-step procedure including chromatography on Bio-Gel P-150, chromatography on DEAE-Sephadex A-50 and chromatography on Sephadex G-100. Three CMCase components ; F-I-Ia, F-I-Ib and F-II-Ia were prepared. No enzyme activity toward avicel could be detected in these components. Similarly, there was no ${\beta}-glucosidase$ activity. pH-optima of the three components were all 5.0 in acetate buffer. Temperature-optima for the activities of F-I-Ia, F-Ib and F-II-Ia were $45^{\circ}C,\;40^{\circ}C\;and\;50^{\circ}C$, respectively. F-II-Ia was shown to be more thermostable than the other two components. F-II-Ia was proved to have quite a different substrate specificity and action property and action property from those of F-I-Ia and F-I-Ib by product analysis on liquid chromatography.

• Journal of Technology Innovation
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• v.27 no.2
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• pp.73-98
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• 2019

This study aims to contribute to the improvement of the patent activity of small and medium-sized enterprises in the future by proposing five strategies for enhancing customized patent activity for small and medium-sized enterprises under the assumption that patent application affects firm performance. The subjects of this study were 25 years of active patent activity, using patent activity statistics for 5 years from 2010 and statistical data of business performance based on 2014. The research procedure was conducted in the order of DEA-BCC efficiency analysis, patent activity analysis, and patent portfolio analysis, and then propose a strategy to improve patent activity. As a result of the research, it was confirmed that 5 industries with high patent activity and IRS type have a substantial effect on improving corporate performance as an industry with high patent efficiency. On the other hand, there are 12 industries in the form of IRS/DRS, and 8 industries with low patent activity, DRS, CRS/IRS mixed type and IRS, and that the efficiency of the patent may vary depending on the characteristics of each industry.

• Educational Technology International
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• v.11 no.1
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• pp.27-46
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• 2010

The purpose of this paper is to apply the newly developed SICAT teaching and learning model to the actual scene of teaching and learning and draw a point of discussion for utilizing teaching and learning model, by uncovering the satisfaction of students and the inhibiting/facilitating elements when using the model. SICAT(Scientific Inquiry and Creative Activity with Technology; from here on SICAT), a teaching and learning model custom-built for engineering education, was developed, as more and more people paid attention to the demand for creative engineers. It was developed from the basis of PBL(Problem Based Learning), includes three sub-types which can be applied to the actual theory, design, and experimentation fields within engineering education. The three sub-types, which are ARDA(Analysis-Reasoning Activity & Discussion-Argumentation Activity), CoCD (Collaboration Activity & Capstone Design Activity), and ReSh(Reflection Activity & Sharing Activity), respectively support deductive and argumentation activities, creative design and collaboration activities, and retrospection and sharing activities. However, no research has been conducted to investigate whether or not there are inhibiting or facilitating elements in the application procedure, or what the rate of satisfaction for students is, when applying the SICAT model, which was newly developed to innovate existing engineering education, to the actual site of teaching and learning. Therefore, this research applied three types of SICAT teaching and learning models to the theory, design, and experimentation classes at the department of materials science and engineering at Hanyang University for eight weeks. After application, the students, teachers and tutors were surveyed and interviewed, and then the results analyzed in order to uncover inhibiting/facilitating elements and the rate of satisfaction. The satisfaction rate of students from the SICAT teaching and learning model was 3.78(in a perfect score of 5: The A type-3.65, The C type-3.80, The R type-3.90), and inhibiting/facilitating elements were drawn from the aspects of learning activities, support system. In conclusion, they can be contributed for implications of SICAT teaching and learning model universal use at engineering education in University.

• IE interfaces
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• v.9 no.3
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• pp.236-248
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• 1996

Most of business enterprises today struggle with the coordination of work which is one of the most challenging problems in the business environment. And the situation becomes worse by the increasing complexity and uncertainty. In this paper, suitability of workflow technology for the modelling, optimization, and automation in the complex business structures is discussed. Also, in the abscence of a fully fledged method for analysis and design of workflow systems, this paper proposes a method, ETM(Extended Trigger Modelling), which can solve several problems in other methods. The proposed ETM approach consists of three stages such as situation analysis stage, activity coordination stage and implementation preparation stage. A modelling case of the proposed procedure is also presented.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.35 no.11B
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• pp.1738-1747
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• 2010

This report shows the analysis and design of tactical decision automation procedure and the result of core algorithm. Expecially scheme of analysis and design includes result of tactical decision supporting procedure analysis for target engagement to fire in refer to AFATDS. Tactical decision automation procedure has three phases like target analysis, target priority, fire unit decision, fire method and attack method. Target analysis creates base information to decide priorities and attack methods through target activity, size and protection status. Target priority and fire unit decision judge target priority and fire unit with unit status and operation mission basis of target priority. Fire unit and Attack method decide fire style according to the kind of fire and ammunition for effective firing achievement. Finally, we show the effective tactical decision automation procedure through making the algorithm of priority and air control.

• 대한공업교육학회지
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• v.39 no.1
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• pp.66-84
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• 2014

The purpose of this study is to develop STEAM hand-on activity task for middle school manufacturing & automation unit. This study was conducted following three stages. First of all, I carried out documents research and requirements analysis. And the goals for STEAM hand-on activity were set at this stage. Second, topics for STEAM hand-on activity were selected, and the organized for designing hand-on activity related STEAM in the development step. Finally, pilot and field test were conducted in order to amend and/or complement in improvement step. The theme and/or title of the hand-on activities were 'Making the print using wood', 'Making the close up photography & telephoto lens for smart phone'. The STEAM hand-on activities were designed for ten hours for each subject respectively. Each hand-on activity consists of problem situation, objectives statement, materials and tools, an evaluating criteria, related knowledge, portfolio and so on.

• Journal of Life Science
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• v.15 no.5 s.72
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• pp.779-782
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• 2005

Cell-free expression is a powerful tool for rapid protein analysis, enabling an efficient identification of gene without cumbersome procedure of transformation and cell culture. Epoxide hydrolase (EH) gene of Rhodotorula glutinis was simply amplified by PCR, and the resultant gene was expressed in vitro using a coupled Transcription/translation system. The cell-free expressed EH protein mixture exhibited the enantioselective hydrolysis activity toward (R)-styrene oxide, representing that cell-free protein synthesis system can be used for the rapid expression of an enantioselective enzyme for an efficient identification of the chiral activity.

• Journal of Korean Home Economics Education Association
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• v.2 no.1
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• pp.123-142
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• 1990

The aim of this study is to apply lesson design of high school home economics to model which consists of six steps of instructional procedure among various models for instructional design, Through thesis, central content of each step was written as follows; 1) At the step of establishment of terminal instructional objectives, educational objectives, terminal instructional objectives was analyzed and specific instructional objectives was extracted. 3) At the step of diagnosis of entering behavior, ability of prior learning and ability of preliminary learning were evaluated, which was used as establishing instructional strategy and doing individual guidance. 4)At the step of determining instructional strategy, instructional strategy should be extracted by considering instructional event which is adequate to content of instruction. Instructional strategy consists of five main factor, factors, those are, activity of introduction of instruction, presentation of content of instruction and information, participation of learner and learning activity, identification of learning result, and subsequent treatment after evaluation. 5)Specific activity at the step of selection and development of instructional media consists of reidentification of instructional objectives determination of learning type, drawing up instructional media analysis sheet, synthesis of selected media, and explanatory note of selected media. In order to increase efficiency of instruction at the step of determining instructional strategy, this step should be considered simultaneously. 6)The step of try-out and evaluation of instructional design should be efficient when designed instruction was inputted at class after evaluation of each step of instructional design.

• Korean Journal of Pharmacognosy
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• v.20 no.2
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• pp.101-109
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• 1989

Purification of polyphenol oxidase(PPO) from Korean native tobacco variety leaves was carried out through the procedure of acetone preciptation, ammonium sulfate fractionation, and Sephadex G-150 gel filtration, resulting in a 84-fold increase in specific activity. The enzyme was stable in a range of pH 7.5 to 8.0 with an optimum of pH 7.5. The optimum temperature for the enzymic reaction was about $60^{\circ}$. It was thermostable with a half-life equal to 20 min at $70^{\circ}$. Km values for (+)-catechin and pyrogallol were $1.6{\times}10^{-3}$ and $0.5{\times}10^{-3}M$, respectively. It possesses high catecholase activity but little or no cresolase activity. Lineweaver-Burk analysis of inhibition data revealed that the inhibition of (+)-catechin oxidation by potassium cyanide, 4-nitrocatechol, cystein and 2-mercaptoethanol was competitive with Ki values of $1.1{\times}10^{-6}$, $1.8{\times}10^{-6}$, $8.9{\times}10^{-6}$ and $1.3{\times}10^{-5}$, respectively.

• Proceedings of the Acoustical Society of Korea Conference
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• 1998.08a
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• pp.142-147
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• 1998

Recently, 8 kb/s CS-ACELP coder of G.729 is atandardized by ITU-T SG15 and it has been reported that the speech quality of G729 is better than or equal to that of 32kb/s ADPCM. However G.729 is the fixed rate speech coder, and it does not consider the property of voice activity in mutual conversation. If we use the voice activity, we can reduce the average bit rate in half without any degradations of the speech quality. In this paper, we propose an efficient variable rate algorithm for G.729. The variable rate algorithm consists of two main subjects, the rate determination algorithm and algorithm, we combine the energy-thresholding method, the phonetic segmentation method by integration of various feature parameters obtained through the analysis procedure, and the variable hangover period method. Through the analysis of noise features, the 1 kb/s sub rate coder is designed for coding the background noise signal. So, we design the 4 kb/s sub rate coder for the unvoiced parts. The performance of the variable rate algorithm is evaluated by the comparison of speed quality and average bit rate with G.729. Subjective quality test is also done by MOS test. Conclusively, it is verified that the proposed variable rate CS-ACELP coder produced the same speech quality as G.729, at the average bit rate of 4.4 kb/s.