• Title/Summary/Keyword: Problem Space

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Production of Transgenic Cattle by Non-surgical Embryo Transfer (비외과적 수정란 이식에 의한 형질전환 소 생산 기술)

  • Uhm, Sang Jun;Yang, Jung Seok;Lee, Su Min;Joe, So Young;Heo, Young-Tae;Xu, Yong-Nan;Koo, Bon Chul;Cheong, Ki Soo;Kim, Kwang Jae;Kim, Ji Tae;Kim, Nam-Hyung;Ko, Dae-Hwan
    • Journal of Embryo Transfer
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    • v.28 no.3
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    • pp.169-175
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    • 2013
  • Recently, the transgenic animal production technique is very important for the production of bio-parmaceutical as animal bio-reactor system. However, the absence of survival evaluation in vitro produced transgenic embryos has been a problem of the low productivity of transgenic animal because of absent of pre-estimate of pregnancy after transgenic embryos transferred into recipient. Therefore, this study is conducted to improve efficiency of transgenic cattle production by improving the non-surgical embryo transfer (ET) method. Transgenic bovine embryos were produced by injection of feline immunodeficiency virus enhanced green fluorescent protein (FIV-EGFP) lentiviral vector into perivitelline space of in vitro matured MII stage oocytes, and then in vitro fertilization (IVF) was occured. Normal IVF and EGFP expressing blastocysts were transferred into recipients. Results indicated that 2 expanded blastocysts (34.7%) transferred group showed significantly (P<0.05) higher pregnancy rate than 1 expanded blastocyst (26.8%) transferred group. In case of parity of recipient, ET to heifer (34.9%) showed significantly (P<0.05) higher pregnancy rate than ET to multiparous recipient (21.2%). However, there are no significant differences of pregnancy rate between natural induced estrus and artificial induced estrus groups. Significantly (P<0.05) higher pregnancy rate was obtained from recipient group which have normal corpus luteum with crown group (34.8%) than normal corpus luteum without crown (13.6%). Additionally, treatment of $100{\mu}g$ Gn-RH injection to recipient group (38.6%) 1 day before ET significantly (P<0.05) increase pregnancy rate than non- Gn-RH injection to recipient group (38.6%). We also transferred 2 EGFP expressing expanded blastocysts to each 19 recipients, 7 recipients were pregnant and finally 5 EGFP transgenic cattle were produced under described ET condition. Therefore, our result suggested that transfer of 2 good-quality expanded blastocysts to $100{\mu}g$ of Gn-RH injected recipient which have normal corpus luteum with crown is feasible to produce transgenic cattle.

Usefulness of Partial Muscle Flaps and Combination Method for Coverage of Prosthetic Material in Chest Wall Reconstruction (흉벽 재건에서 합성 물질의 피복을 위한 부분 근피판 조합의 효용성)

  • Ryoo, Suk-Tae;Pyon, Jai-Kyong;Lim, So-Young;Mun, Goo-Hyun;Bang, Sa-Ik;Oh, Kap-Sung
    • Archives of Plastic Surgery
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    • v.38 no.3
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    • pp.228-234
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    • 2011
  • Purpose: Reconstruction of chest wall has always been a challenging problem. Muscle flaps for chest wall reconstruction have been helpful in controling infection, filling dead space and covering the prosthetic material in this challenge. However, when we use muscle flaps, functional and cosmetic donor site morbidities could occur. The authors applied and revised various partial muscle flaps and combination use of them to cover the prosthetic material for the chest wall reconstruction and evaluated the usefulness of partial muscle flaps. Methods: This study included 7 patients who underwent chest wall reconstruction using partial muscle flap to cover prosthetic material from 2004 to 2008. The pectoralis major muscle was used in anterior 2/3 parts of it leaving lateral 1/3 parts of it. The anterior 2/3 parts of the pectoralis major muscle were used while lateral 1/3 parts were left. In case of the rectus abdominis muscle flap, we used upper half of it, or we dissected it around its origin and then advanced to cover the site. The latissimus dorsi muscle flap was elevated with lateral portion of it along the descending branch of the thoracodorsal artery. If single partial muscle flap could not cover whole prosthetic material, it would be covered with combination of various partial muscle flaps adjacent to the coverage site. Results: Flap coverage of the prosthetic material and chest wall reconstructions were successfully done. There occurred no immediate and delayed post operative complications such as surgical site infection, seroma, deformity of donor site and functional impairment. Conclusion: When we use the muscle flaps to cover prosthetic material for chest wall reconstruction, use of the partial muscle flaps could be a good way to reduce donor site morbidity. Combination of multiple partial flaps could be a valuable and good alternative way to overcome the disadvantages of partial muscle flaps such as limitation of volume and size as well as flap mobility.

A Study on the Temperature Distribution of Rock Mass at KAERI Underground Research Tunnel: Verification on the Result of Borehole Heater Test (지하처분연구시설(KURT) 내 암반의 온도 분포에 관한 연구 : 시추공히터시험 결과의 검증)

  • Yoon, Chan-Hoon;Choi, Young-Chul;Kwon, Sang-Ki;Choi, Heui-Joo
    • Tunnel and Underground Space
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    • v.23 no.4
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    • pp.297-307
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    • 2013
  • In this study, the thermal analysis is carried out for a result of borehole heater test using ABAQUS ver 6.10 based on finite element analysis code. Thermal-mechanical rock properties as determined by laboratory tests before the in situ test and characteristics of the atmosphere at the test section are used as the initial condition. When comparing the results of the in situ test and thermal analysis, the temperature of C3 observation hole that is 0.9 m away from the heater showed very similar patterns and figures (about $1.3^{\circ}C$ difference). But the results of the A and B observation hole showed a significant difference around $15^{\circ}C{\sim}20^{\circ}C$. To find the reason for these results, the over-coring is carried out for the A1 and B1 observation holes. As a result of checking the excavated rock core with the naked eye, there is no problem on the number and position of the sensor as the test plan. However the state of cement injection in the observation hole is poor.

Infant and Toddler Group Care at the Mother's Work Place (직장내 탁아프로그램 개발을 위한 기초연구 - 영·유아를 중심으로 -)

  • Zi, Heyon
    • Korean Journal of Child Studies
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    • v.7 no.2
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    • pp.1-15
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    • 1986
  • Korea is a rapidly industrializing nation. As a consequence, more mothers are working outside the home. This trend is likely to continue into the future and raises the problem of infant-toddler care during the mother's working hours. Considering that Korea is making such rapid economic advances, quality care for children of working mothers Is not only desirable but affordable as well. Variety in options for child care is recommended by Beck (1982). Some of the alternatives in child care have been discussed elsewhere (KWDI, 1984). This paper, thus, concentrated on center-based, worksite child care. Because the human environment is the most important aspect of the center, qualified staff and a low ratio of children per staff member is Vital. Philosophy of program and its actualization ensures that the children have a worthwhile experience at the center. The physical plant houses the center and includes provision for space, materials, and equipment for staff and child use in carrying out the program. Adequate health protection is a critical factor in infant-toddler group care programs.

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SLAM Method by Disparity Change and Partial Segmentation of Scene Structure (시차변화(Disparity Change)와 장면의 부분 분할을 이용한 SLAM 방법)

  • Choi, Jaewoo;Lee, Chulhee;Eem, Changkyoung;Hong, Hyunki
    • Journal of the Institute of Electronics and Information Engineers
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    • v.52 no.8
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    • pp.132-139
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    • 2015
  • Visual SLAM(Simultaneous Localization And Mapping) has been used widely to estimate a mobile robot's location. Visual SLAM estimates relative motions with static visual features over image sequence. Because visual SLAM methods assume generally static features in the environment, we cannot obtain precise results in dynamic situation including many moving objects: cars and human beings. This paper presents a stereo vision based SLAM method in dynamic environment. First, we extract disparity map with stereo vision and compute optical flow. We then compute disparity change that is the estimated flow field between stereo views. After examining the disparity change value, we detect ROIs(Region Of Interest) in disparity space to determine dynamic scene objects. In indoor environment, many structural planes like walls may be determined as false dynamic elements. To solve this problem, we segment the scene into planar structure. More specifically, disparity values by the stereo vision are projected to X-Z plane and we employ Hough transform to determine planes. In final step, we remove ROIs nearby the walls and discriminate static scene elements in indoor environment. The experimental results show that the proposed method can obtain stable performance in dynamic environment.

Information Analyses of Child Who Needs Protection in Busan (요보호아동의 정보화 실태 분석: 부산지역을 중심으로)

  • 김옥희
    • Journal of Families and Better Life
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    • v.20 no.5
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    • pp.149-166
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    • 2002
  • In this study, 1 have analyzed the state of the use of information technology by the at-risk children who are residing in Husan metropolitan area. The ultimate goal of this study is to help ensure the healthy development of the children and improve the quality of their lives in this information-oriented society. For this research, 1 surveyed 183 children living in an institution or a low-income family in Busan metropolitan area. The survey questionnaire was designed to obtain information on the respondents, socio-demographic characteristics, computer-related conditions of the immediate and social environments, patterns of computer and Internet use, their experience and degree of so called, Net-generation syndrome, Internet addiction disorder, changes in social and familial life caused by Internet use, and exposure to pornographic materials. The results show that 95.9 % of at-risk children in Husan own a computer, 60.9 % of those computers being Pentium Ⅲ. A majority of the children (74.9 %) are connected to Internet at a high-speed, using such devices as LAN, ADSL or cables. About seventy-six percent of the children have used a PC and Internet less than two years. In other words, a majority of them have not used those for a long period of time. The main results of the research are summarized in the following: There is a significant difference among at-risk children in terms of experiences in computer and Internet use according to their grade levels. There is a gender difference in the amount of time spent on computer and Internet. Boys spend more time every week on computer and Internet than girls do. There is a significant difference in the pattern of computer and Internet use, according to the type of their risk, and grade level. The so-called 'Net Generation Syndrome' is not serious in this group. A majority of the at-risk children think that Internet is more interesting than TV. They hardly shop on Internet, and they don think the advertisements on Internet are a serious problem. Also, unlike many people assumptions, their desire to communicate through on-line chatting was very low. They do not tend to buy computer game programs, and Internet Addiction Disorder was not pronounced among them. The at-risk children rarely had an exposure to pornographic materials in the cyber space. However, the exposure was more frequent for institutionalized children than children in low-income families. These results point to a need for a policy to improve the welfare of at-risk children and guarantee their right to information.

NOVEL TREATMENT MODALITY OF IMPLANT-RETAINED OVERDENTURE FOR IMMEDIATE FUNCTION IN COMPLETELY EDENTULOUS PATIENTS (무치악 환자에서 하악 임플랜트 지지 피개의치에 의한 즉시 하중 부담에 관한 연구)

  • Kim, Jang-Wook;Kim, Hyeong-Seob;Baik, Jin;Kwon, Kung-Rock
    • The Journal of Korean Academy of Prosthodontics
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    • v.45 no.1
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    • pp.34-47
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    • 2007
  • Statement of problem: Conventional mandibular complete denture lacks in support and stability for edentulous patients to use. Purpose: To enhance support and stability of mandibular complete denture, 2 implants were inserted on the inter-mental foramina space of edentulous patients. With 2 magnetic attachments on the inner side of the overdenture, we gave immediate loads and evaluated the stability of the implants, the responses of the supporting tissues, and the satisfaction of the patients. Material & methods: 6 edentulous patients (5 male, 1 female) each recieved 2 SLA implants in the inter-mental foramina region and implants were immediately loaded with overdenture with magnet attachments. To evaluate the implants stability and the peri-implant tissues, clinical exams, RFA tests and radiographic exams were preformed at the 1 week, 2wks, 6wks, 12wks, and 24wks. post- surgery. Results: The mean surgery time was $45.7{\pm}7.7mins$., while the denture delivery time was $45.5{\pm}12.6mins$. Only 2 of 14 implants were failed. Survived implants that remained were clincally and radiographically stable. Mean ISQ values were relatively stable, showing $69.71{\pm}5.55$, $69.00{\pm}9.48$, $67.92{\pm}7.86$, $67.92{\pm}9.58$, $70.08{\pm}7.61$, $71.92{\pm}6.43$ at the 1 wk, 2 wks, 6 wks, 12 wks, 24 wks. follow up check. Crestal bone changes were $-1.18mm{\pm}0.68mm$, $-1.35{\pm}0.69mm$, $-1.47{\pm}0.68mm$ at the 6wks, 12wks, 24wks. follow up check. Bleeding on probing(BOP index) was not significant. Conclusion: Mandibular ovedentures with 2 magnetic attachments over two interforaminal implants on edentulous patients for immediate function is a recommendable novel treatment for edentulous patients which shows stability on the implants and supporting tissue.

Characteristics for Co-digestion of Food Waste and Night Soil using BMP Test (BMP실험을 이용한 음식물폐기물 및 분뇨의 병합소화 특성)

  • Cho, Jinkyu;Kim, Hyungjin;Oh, Daemin
    • Journal of the Korean GEO-environmental Society
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    • v.15 no.9
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    • pp.13-18
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    • 2014
  • BMP test was carried out to evaluate the characteristics for co-digestion of night soil and food waste. 6 types of sludge were tested in 30 days which were raw, excess, digested, night soil/septic tank (1:1), food waste (food : dilution water = 1:1), and mixed sludge. Bio gas was produced actively after 2 days, and continued in 2 weeks. Gas generation amount was decreased rapidly after considerable space of time. Especially maximum productivity of gas was shown in 7~8 days. The ultimate methane yields of raw, excess, digested, night soil/septic tank, food waste, and mixed sludge were 64.63, 67.49, 66.45, 72.44, 107.85, and 46.71 mL $CH_4/g$ VS respectively from Modified Gompertz model. The lag growth phase time and maximum specific methane production rate of mixed sludge were 1.88 day and 80.4 mL/day respectively. The methane potential of mixed sludge was higher than individual sludge. So high methane potential was expected by controlling mixing ratio of food waste. Besides stable operation of digestion tank and the solution of oligotrophic problem were possible.

Retrospects and Prospects of Sixty Years of Geographical Studies in Korea (한국의 지리학연구 60년 회고와 전망)

  • Park, Sam-Ock
    • Journal of the Korean Geographical Society
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    • v.40 no.6 s.111
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    • pp.770-788
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    • 2005
  • In this article, major research trends are examined mainly from articles published in Journal of the Korean Geography, academic society journals of sub-fields in geography and Ph.D. dissertations in geography of universities in Korea. Based on the research trends up to current point, issues and research directions in order to raise the status of Korean Geography in the twenty first century are prospected. Sixty years of Korean Geography can be divided into 4 periods such as before 1970s, during the 1980s, during the 1990s, and the period after the 2001. Each period of time revealed distinct aspects of development both quantitatively and qualitatively. For the last 60 years, the study of Korean Geography has gone through enormous changes in the number of articles with dynamics and variety in contents and methodology, breathing with the changes of the Korean society. Experiencing urbanization and industrialization along with rapid economical growth, the study of Korean Geography has progressed by analyzing the issues and problems of national space and conducting various researches from the case studies for problem-solving to the theoretical and policy oriented studies. The key-words such as 'knowledge based information society', 'aged society', and 'the era of globalization' will be realized in the twenty flit century. And environment-friendly policies for sustainable development will be more stressed in a globalized world. In order to provide research methods and alternatives suitable for newly transformed global society and for utilizing the essence of Geography in the future, the studies on Korean Geography should actively focus on integrative studies between physical and human geography, on interdisciplinary studies, on regional studies related to foreign countries and North Korea, and on the establishment of Korean geographical theory and model.

Indicating the Problem of Shielding Design and the Way of Estimating Radiation Leakage for CT Rooms located through Survey of Radiation Leakage : in the case of Busan and Gyung-nam Area (방사선 누설선량 조사를 통한 방어시설과 누설선량 평가방법에 대한 문제점 연구 : 부산, 경남 지역 CT실을 중심으로)

  • Yang, Won Seok;Choi, Jun-Hyeok;Shin, Woon-Jae;Min, Byung-In
    • The Journal of the Korea Contents Association
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    • v.13 no.11
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    • pp.768-777
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    • 2013
  • The purpose of this study is to minimize radiation exposure to the workers and public members during CT examination. The objects are seven of the CT rooms in university hospitals and four of the CT rooms in clinics located in Busan and Gyung-nam area. The places of measurement for radiation leakage are 1) 3 m above the ground of shielding wall in the control room 2) particular space in the control room 3) worker's gate in the control room 4) the patient gate. Its values were calculated maximum leakage radiation per week(MLRW). As a result, the worker's gate of M clinic displayed the highest dose. When it was calculated by MLRW in classic method, it showed 1) $5.97{\pm}0.23$, 2) $0.50{\pm}0.02$, 3) $10.00{\pm}0.11$, 4) $2.37{\pm}0.47$ mR/week. All of them did not exceed limit for maximum permissible dose per week(MPDW). However, When MLRW of M clinic was calculated by empirical method, its value displayed $118.31{\pm}17.72$ mR/week.(MPDW>100 mR/week). Radiation leakage influenced in the control room(p<0.05). Therefore, The way of calculating MLRW must be developed and shielding wall in control room is designed 3 m above the ground for reducing dangerous of leakage radiation.