한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제28권3호
  • /
  • Pages.169-175
  • /
  • 2013
  • /
  • 2508-755X(pISSN)
  • /
  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
권호 표지
DOI QR코드

DOI QR Code

비외과적 수정란 이식에 의한 형질전환 소 생산 기술

Production of Transgenic Cattle by Non-surgical Embryo Transfer

  • 엄상준 (상지영서대학교 동물생명산업과) ;
  • 양정석 (상지영서대학교 동물생명산업과) ;
  • 이수민 (상지영서대학교 동물생명산업과) ;
  • 조소영 (상지영서대학교 동물생명산업과) ;
  • 허영태 (충북대학교 축산학과) ;
  • 허영남 (충북대학교 축산학과) ;
  • 구본철 (대구가톨릭대학교 의학과) ;
  • 정기수 (강원도가축위생시험소남부지소) ;
  • 김광재 (강원도가축위생시험소남부지소) ;
  • 김지태 (강원도가축위생시험소남부지소) ;
  • 김남형 (충북대학교 축산학과) ;
  • 고대환 (상지영서대학교 동물생명산업과)
  • Uhm, Sang Jun (Department of Animal Science and Biotechnology, Sangji Youngseo College) ;
  • Yang, Jung Seok (Department of Animal Science and Biotechnology, Sangji Youngseo College) ;
  • Lee, Su Min (Department of Animal Science and Biotechnology, Sangji Youngseo College) ;
  • Joe, So Young (Department of Animal Science and Biotechnology, Sangji Youngseo College) ;
  • Heo, Young-Tae (Department of Animal Sciences, Chungbuk National University) ;
  • Xu, Yong-Nan (Department of Animal Sciences, Chungbuk National University) ;
  • Koo, Bon Chul (Department of Physiology, Catholic University of Daegu School of Medicine) ;
  • Cheong, Ki Soo (South Branch of Gangwondo Veterinary Service LAB) ;
  • Kim, Kwang Jae (South Branch of Gangwondo Veterinary Service LAB) ;
  • Kim, Ji Tae (South Branch of Gangwondo Veterinary Service LAB) ;
  • Kim, Nam-Hyung (Department of Animal Sciences, Chungbuk National University) ;
  • Ko, Dae-Hwan (Department of Animal Science and Biotechnology, Sangji Youngseo College)
  • 투고 : 2013.07.05
  • 심사 : 2013.08.05
  • 발행 : 2013.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Recently, the transgenic animal production technique is very important for the production of bio-parmaceutical as animal bio-reactor system. However, the absence of survival evaluation in vitro produced transgenic embryos has been a problem of the low productivity of transgenic animal because of absent of pre-estimate of pregnancy after transgenic embryos transferred into recipient. Therefore, this study is conducted to improve efficiency of transgenic cattle production by improving the non-surgical embryo transfer (ET) method. Transgenic bovine embryos were produced by injection of feline immunodeficiency virus enhanced green fluorescent protein (FIV-EGFP) lentiviral vector into perivitelline space of in vitro matured MII stage oocytes, and then in vitro fertilization (IVF) was occured. Normal IVF and EGFP expressing blastocysts were transferred into recipients. Results indicated that 2 expanded blastocysts (34.7%) transferred group showed significantly (P<0.05) higher pregnancy rate than 1 expanded blastocyst (26.8%) transferred group. In case of parity of recipient, ET to heifer (34.9%) showed significantly (P<0.05) higher pregnancy rate than ET to multiparous recipient (21.2%). However, there are no significant differences of pregnancy rate between natural induced estrus and artificial induced estrus groups. Significantly (P<0.05) higher pregnancy rate was obtained from recipient group which have normal corpus luteum with crown group (34.8%) than normal corpus luteum without crown (13.6%). Additionally, treatment of $100{\mu}g$ Gn-RH injection to recipient group (38.6%) 1 day before ET significantly (P<0.05) increase pregnancy rate than non- Gn-RH injection to recipient group (38.6%). We also transferred 2 EGFP expressing expanded blastocysts to each 19 recipients, 7 recipients were pregnant and finally 5 EGFP transgenic cattle were produced under described ET condition. Therefore, our result suggested that transfer of 2 good-quality expanded blastocysts to $100{\mu}g$ of Gn-RH injected recipient which have normal corpus luteum with crown is feasible to produce transgenic cattle.

키워드

참고문헌

  1. Betteridge KJ, Eaglesome MD, Randall GCB and Mitchell D. 1980. Collection, description and transfer of embryos from cattle 10-16 days after oestus. J. Reprod. Fert. 59: 205-216. https://doi.org/10.1530/jrf.0.0590205
  2. Cabot RA, Kuhholzer B, Chan AW, Lai L, Park KW, Chong KY, Schatten G, Murphy CN, Abeydeera LR, Day BN and Prather RS. 2001. Transgenic pigs produced using in vitro matured oocytes infected with a retroviral vector. Anim. Biotechnol. 12: 205-214. https://doi.org/10.1081/ABIO-100108347
  3. Chan AWS, Homan EJ, Ballou LU, Burns JC and Bremel RD. 1998. Transgenic cattle produced by reversetranscribed gene transfer in oocytes. Proc. Natl. Acad. Sci. USA 95: 14028-14033.
  4. Choe CY, Son JK, Cho SR, Kang DW, Yeon SH, Choi SH, Choi SH, Kim NT, Kim JB, Jung YS, Kim SJ, Jung JW, Bok NH, Yoo YH and Son DS. 2010. Effects of pregnant rate after embryo transfer in oxygen consumption of embryos in Korean cattle. Korean J. Emb. Trans. 25: 145-148.
  5. Choe CY, Son JK, Cho SR, Kang DW, Yeon SH, Choi SH, Kim NT, Jung YS, Kim SJ, Jung JW, Bok NH, Choi JS and Son DS. 2011. Effects of corpus luteum grade of estrus synchronized recipients on pregnancy rate following embryo transfer in Korean cattle. Korean J. Emb. Trans. 26: 33-36.
  6. Heyman Y. 1985. Factors affecting the survival of whole and half-embryos transferred in cattle. Theriogenology 23:63-75. https://doi.org/10.1016/0093-691X(85)90073-1
  7. Hofmann A, Kessler B, Ewerling S, Weppert M, Vogg B, Ludwig H, Stojkovic M, Boelhauve M, Brem G, Wolf E and Pfeifer A. 2003. Efficient transgenesis in farmanimals by lentiviral vectors. EMBO Rep. 4: 1054-1060. https://doi.org/10.1038/sj.embor.7400007
  8. Hofmann A, Zakhartchenko V, Weppert M, Sebald H, Wenigerkind H, Brem G, Wolf E and Pfeifer A. 2004 Generation of transgenic cattle by lentiviral gene transfer into oocytes. Biol. Reprod. 71: 405-409. https://doi.org/10.1095/biolreprod.104.028472
  9. Jaenisch R. 1976. Germ line integration and Mendelian transmission of the exogenous Moloney leukemia virus. Proc. Natl. Acad. Sci. USA 73: 1260-1264. https://doi.org/10.1073/pnas.73.4.1260
  10. Jaenisch R. 2002. Nuclear cloning, embryonic stem cells, and transplantation therapy. Harvey Lect. 98: 145-171.
  11. Kim HR, Kim DI, Park NH, Kim CK, Chung YC, Yoon JT and Jeon GJ. 1998. Studied on the improvement embryo transfer efficiency in Korean cattle I. Effect of recipient conditions on pregnancy rate after embryo transfer. Korean J. Emb. Trans. 13: 61-67.
  12. Kim HR, Kim DI, Won YS, Chung YC, Lee KS, Suh HW and Park CS. 1998. Studied on the improvement embryo transfer efficiency in Korean cattle I. Effect of embryo conditions on pregnancy rate after embryo transfer. Korean J. Emb. Trans. 13: 53-60.
  13. Kim YJ, Park H, Lee HL, Shin DS, Jo SW, Kim YS and Kim SH. 2008. Results of embryo transfer with Hanwoo embryos produced in-vivo or in-vitro to Holstein cows as recipients. Korean J. Emb. Trans. 23: 167-175.
  14. Kim T, Leibfried-Rutledge ML and First NL. 1993. Gene transfer in bovine blastocysts using replication-defective retroviral vectors packaged with Gibbon ape leukemia virus envelopes. Mol. Reprod. Dev. 35: 105-113. https://doi.org/10.1002/mrd.1080350202
  15. Koo BC, Kwon MS, Roh JY, Kim M, Kim JH and Kim T. 2012. Quantitative analysis of tetracycline-inducible expression of the green fluorescent protein gene in transgenic chickens. J. Reprod. Dev. 58: 672-677. https://doi.org/10.1262/jrd.2012-092
  16. Lois C, Hong EJ, Pease S, Brown EJ and Baltimore D. 2002. Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors. Science 295: 868-872. https://doi.org/10.1126/science.1067081
  17. Oh SJ, Yang BS, Im GS, Yang BC, Seong HJ, Park YY and Kim KN. 2001. Effects of characteristics of corpus luteum and serum metabolites on pregnancy rate following embryo transfer in Hanwoo cow. Korean J. Animal. Reprod. 25: 9-17.
  18. Palmiter RD, Brinster RL, Hammer RE, Trumbauer ME, Rosenfeld MG, Birnberg NC and Evans RM. 1982. Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes. Nature 300: 611-615. https://doi.org/10.1038/300611a0
  19. Rideout WM, Eggan K and Jaenisch R. 2001. Nuclear cloning and epigenetic reprogramming of the genome. Science 293: 1093-1098. https://doi.org/10.1126/science.1063206
  20. Schnieke AE, Kind AJ, Ritchie WA, Mycock K, Scott AR, Ritchie M, Wilmut I, Colman A and Campbell KH. 1997. Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts. Science 278: 2130-2133. https://doi.org/10.1126/science.278.5346.2130
  21. Wall RJ. 1996. Transgenic livestock: progress and prospects for the future. Theriogenology 45: 57-68. https://doi.org/10.1016/0093-691X(95)00355-C
  22. Wright JM. 1981. Non-surgical transfer in cattle : embryorecipient interaction. Theriogenology 15: 43-46. https://doi.org/10.1016/S0093-691X(81)80017-9
  23. Xu YN, Uhm SJ, Koo BC, Kwon MS, Roh JY, Yang JS, Choi HY, Heo YT, Cui XS, Yoon JH, Ko DH, Kim T and Kim NH. 2013. Production of transgenic Korean native cattle expressing enhanced green fluorescent protein using a FIVbased lentiviral vector injected into MII oocytes. J. Genet. Genomics 40: 37-43. https://doi.org/10.1016/j.jgg.2012.11.001