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http://dx.doi.org/10.12750/JET.2013.28.3.169

Production of Transgenic Cattle by Non-surgical Embryo Transfer  

Uhm, Sang Jun (Department of Animal Science and Biotechnology, Sangji Youngseo College)
Yang, Jung Seok (Department of Animal Science and Biotechnology, Sangji Youngseo College)
Lee, Su Min (Department of Animal Science and Biotechnology, Sangji Youngseo College)
Joe, So Young (Department of Animal Science and Biotechnology, Sangji Youngseo College)
Heo, Young-Tae (Department of Animal Sciences, Chungbuk National University)
Xu, Yong-Nan (Department of Animal Sciences, Chungbuk National University)
Koo, Bon Chul (Department of Physiology, Catholic University of Daegu School of Medicine)
Cheong, Ki Soo (South Branch of Gangwondo Veterinary Service LAB)
Kim, Kwang Jae (South Branch of Gangwondo Veterinary Service LAB)
Kim, Ji Tae (South Branch of Gangwondo Veterinary Service LAB)
Kim, Nam-Hyung (Department of Animal Sciences, Chungbuk National University)
Ko, Dae-Hwan (Department of Animal Science and Biotechnology, Sangji Youngseo College)
Publication Information
Journal of Embryo Transfer / v.28, no.3, 2013 , pp. 169-175 More about this Journal
Abstract
Recently, the transgenic animal production technique is very important for the production of bio-parmaceutical as animal bio-reactor system. However, the absence of survival evaluation in vitro produced transgenic embryos has been a problem of the low productivity of transgenic animal because of absent of pre-estimate of pregnancy after transgenic embryos transferred into recipient. Therefore, this study is conducted to improve efficiency of transgenic cattle production by improving the non-surgical embryo transfer (ET) method. Transgenic bovine embryos were produced by injection of feline immunodeficiency virus enhanced green fluorescent protein (FIV-EGFP) lentiviral vector into perivitelline space of in vitro matured MII stage oocytes, and then in vitro fertilization (IVF) was occured. Normal IVF and EGFP expressing blastocysts were transferred into recipients. Results indicated that 2 expanded blastocysts (34.7%) transferred group showed significantly (P<0.05) higher pregnancy rate than 1 expanded blastocyst (26.8%) transferred group. In case of parity of recipient, ET to heifer (34.9%) showed significantly (P<0.05) higher pregnancy rate than ET to multiparous recipient (21.2%). However, there are no significant differences of pregnancy rate between natural induced estrus and artificial induced estrus groups. Significantly (P<0.05) higher pregnancy rate was obtained from recipient group which have normal corpus luteum with crown group (34.8%) than normal corpus luteum without crown (13.6%). Additionally, treatment of $100{\mu}g$ Gn-RH injection to recipient group (38.6%) 1 day before ET significantly (P<0.05) increase pregnancy rate than non- Gn-RH injection to recipient group (38.6%). We also transferred 2 EGFP expressing expanded blastocysts to each 19 recipients, 7 recipients were pregnant and finally 5 EGFP transgenic cattle were produced under described ET condition. Therefore, our result suggested that transfer of 2 good-quality expanded blastocysts to $100{\mu}g$ of Gn-RH injected recipient which have normal corpus luteum with crown is feasible to produce transgenic cattle.
Keywords
embryo transfer; expand blastocyst; pregnancy; EGFP; transgenic cattle;
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