• 제목/요약/키워드: ProSe

검색결과 160건 처리시간 0.027초

Mechanisms of Resorcinol Antagonism of Benzo[a]pyrene-Induced Damage to Human Keratinocytes

  • Lee, Seung Eun;Kwon, Kitae;Oh, Sae Woong;Park, Se Jung;Yu, Eunbi;Kim, Hyeyoun;Yang, Seyoung;Park, Jung Yoen;Chung, Woo-Jae;Cho, Jae Youl;Lee, Jongsung
    • Biomolecules & Therapeutics
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    • 제29권2호
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    • pp.227-233
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    • 2021
  • Benzo[a]pyrene (B[a]P) is a polycyclic aromatic hydrocarbon and ubiquitous environmental toxin with known harmful effects to human health. Abnormal phenotypes of keratinocytes are closely associated with their exposure to B[a]P. Resorcinol is a component of argan oil with reported anticancer activities, but its mechanism of action and potential effect on B[a]P damage to the skin is unknown. In this study, we investigated the effects of resorcinol on B[a]P-induced abnormal keratinocyte biology and its mechanisms of action in human epidermal keratinocyte cell line HaCaT. Resorcinol suppressed aryl hydrocarbon receptor (AhR) activity as evidenced by the inhibition of B[a]P-induced xenobiotic response element (XRE)-reporter activation and cytochrome P450 1A1 (CYP1A1) expression. In addition, resorcinol attenuated B[a]P-induced nuclear translocation of AhR, and production of ROS and pro-inflammatory cytokines. We also found that resorcinol increased nuclear factor (erythroid-derived 2)-like 2 (Nrf2) activity. Antioxidant response element (ARE)-reporter activity and expression of ARE-dependent genes NAD(P)H dehydrogenase [quinone] 1 (NQO1), heme oxygenase-1 (HO-1) were increased by resorcinol. Consistently, resorcinol treatment induced nuclear localization of Nrf2 as seen by Western analysis. Knockdown of Nrf2 attenuated the resorcinol effects on ARE signaling, but knockdown of AhR did not affect resorcinol activation of Nrf2. This suggests that activation of antioxidant activity by resorcinol is not mediated by AhR. These results indicate that resorcinol is protective against effects of B[a]P exposure. The mechanism of action of resorcinol is inhibition of AhR and activation of Nrf2-mediated antioxidant signaling. Our findings suggest that resorcinol may have potential as a protective agent against B[a]P-containing pollutants.

The antioxidant icariin protects porcine oocytes from age-related damage in vitro

  • Yoon, Jae-Wook;Lee, Seung-Eun;Park, Yun-Gwi;Kim, Won-Jae;Park, Hyo-Jin;Park, Chan-Oh;Kim, So-Hee;Oh, Seung-Hwan;Lee, Do-Geon;Pyeon, Da-Bin;Kim, Eun-Young;Park, Se-Pill
    • Animal Bioscience
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    • 제34권4호
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    • pp.546-557
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    • 2021
  • Objective: If fertilization does not occur within a specific period, the quality of unfertilized oocytes in the oviduct (in vivo aging) or in culture (in vitro aging) will deteriorate over time. Icariin (ICA), found in all species of Epimedium herbs, has strong antioxidant activity, and is thought to exert anti-aging effects in vitro. We asked whether ICA protects oocytes against age-related changes in vitro. Methods: We analyzed the reactive oxygen species (ROS) levels and expression of antioxidant, maternal, and estrogen receptor genes, and along with spindle morphology, and the developmental competence and quality of embryos in the presence and absence of ICA. Results: Treatment with 5 μM ICA (ICA-5) led to a significant reduction in ROS activity, but increased mRNA expression of glutathione and antioxidant genes (superoxide dismutase 1 [SOD1], SOD2, peroxiredoxin 5, and nuclear factor erythroid 2-like 2), during aging in vitro. In addition, ICA-5 prevented defects in spindle formation and chromosomal alignment, and increased mRNA expression of cytoplasmic maturation factor genes (bone morphogenetic protein 15, cyclin B1, MOS proto-oncogene, serine/threonine kinase, and growth differentiation factor-9). It also prevented apoptosis, increased mRNA expression of antiapoptotic genes (BCL2-like 1 and baculoviral IAP repeat-containing 5), and reduced mRNA expression of pro-apoptotic genes (BCL2 antagonist/killer 1 and activation of caspase-3). Although the maturation and cleavage rates were similar in all groups, the total cell number per blastocyst and the percentage of apoptotic cells at the blastocyst stage were higher and lower, respectively, in the control and ICA-5 groups than in the aging group. Conclusion: ICA protects oocytes against damage during aging in vitro; therefore, it can be used to improve assisted reproductive technologies.

Anti-inflammatory Effects of Ethanol Extract of Korean Medicinal Plants at Hwaak Mountain in LPS-induced RAW 264.7 Macrophages

  • Kang, Yun-Mi;Jeon, Eun-jin;Chung, Kyung-Sook;Cheon, Se-Yun;Park, Jong Hyuk;Han, Yoo-Chang;An, Hyo-Jin
    • 대한본초학회지
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    • 제32권2호
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    • pp.25-32
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    • 2017
  • Objectives : This study was conducted to investigate candidate materials as anti-inflammatory agent from extracts of Korean medicinal plants in Hwaak mountain. Ligustrum obtusifolium (LO) is a Korea medicinal plants that commonly used for robustness and hemostasis. It has been reported that LO has exhibited anti-ischemic, anti-oxidative, anti-hypolipidemic, anti-tumor and hypoglycemic effects. However, LO has not been previously reported to have an anti-inflammatory effect. Therefore, we have evaluated the anti-inflammatory effects of LO and its underlying molecular mechanisms in LPS-induced RAW 264.7 macrophages. Methods : Cell viability was determined by MTT assay in RAW 264.7 macrophages. Nitric Oxide (NO) was measured with Griess reagent and pro-inflammatory cytokines were detected by ELISA in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Protein expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and p65 subunit of nuclear $factor-{\kappa}B$ ($NF-{\kappa}B$) were determined by Western blot analysis. Results : Among 15 extracts of Korean medicinal plants tested, Ligustrum obtusifolium (LO) showed the inhibition of NO production without cytotoxicity. LO reduced the expression levels of iNOS and COX-2 proteins in LPS-simulated RAW 264.7 macrophages in dose-dependent manner. Consistent with these data, LO inhibited the productions of $TNF-{\alpha}$, IL-6, and $IL-1{\beta}$ in LPS-simulated RAW 264.7 macrophages. Furthermore, LO attenuated the LPS-induced nuclear translocation of p65 $NF-{\kappa}B$ in RAW 264.7 macrophages involving suppression of $NF-{\kappa}B$ activation. Conclusions : Taken together, these results suggest that the anti-inflammatory effects of LO is associated with regulation of inflammatory mediators via inhibition of $NF-{\kappa}B$ activation in LPS-treated RAW 264.7 macrophages.

급성 알코올 투여 백서의 신문혈 자극이 소교세포 활성에 미치는 영향 (Suppression of Microglial Activation by Acute Ethanol Administration through HT7 Stimulation)

  • 서수연;방세권;강석윤;조성진;최광호;류연희
    • Korean Journal of Acupuncture
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    • 제41권2호
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    • pp.33-42
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    • 2024
  • Objectives : The sigma-1 receptor is implicated in stress, depression, psychostimulant sensitization, and addiction vulnerability. Prior studies have indicated that ethanol exposure modulates sigma-1 receptor activity within the Ventral Tegmental Area (VTA). Here, we explore the sub-mechanisms underlying sigma-1 receptor activity induced by HT7 (Shinmun) stimulation in behavioral alterations following acute ethanol (ETOH) administration. Methods : Male Wistar rats were investigated for pro- and anti-inflammatory markers after injection of ETOH (1 g/kg) using cytokine enzyme-linked immunosorbent assay (ELISA)s. After confirming that HT7 stimulation changed the total distance traveled in the open field test (OFT), protein changes in the Ventral tegmental area (VTA) were measured by Western blotting. The expression level of inducible nitric oxide synthase (iNOS) after administration of a sigma-1 receptor antagonist (dihydrobromide 1047; BD1047, 10 mg/kg i.p.) and Shenmen (HT7) stimulation was compared. Results : As a result, acute ETOH administration increased proinflammatory marker levels (TNF-𝛼 and IL-6). HT7 stimulation restored the total distance response after acute ethanol administration. In addition, in the VTA, the levels of a microglial marker (iNOS), sigma-1 receptor and protein kinase C, which are predicted to be involved in up- and downregulation, were restored by HT7 stimulation. In particular, HT7 stimulation modulates iNOS expression through effects similar to BD treatment. This study suggests that the stimulatory effect of HT7 may be driven by microglial activation. Conclusions : Microglial activity is regulated by sigma-1 receptor, and sigma-1 receptor activity is regulated by HT7 stimulation. Significantly, we demonstrate that HT7 stimulation ameliorates behavioral alterations induced by acute ETOH administration through microglial activation within the VTA.

크릴 가공폐기물을 이용한 Carotenoprotein의 추출조건 및 품질안정성에 관한 연구 (Extraction Conditions and Quality Stability of Carotenoprotein from Krill Processing Waste by Proteolytic Enzymes)

  • 김세권;김용태;곽동채;조덕제;이응호
    • 한국수산과학회지
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    • 제23권1호
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    • pp.40-50
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    • 1990
  • 크릴가공시 부산물로 얻어지는 잔사를 보다 효율적으로 이용하기 위하여 크릴가공 잔사로부터 carotenoprotein을 추출하여 이를 식품착색료로 이용할 목적으로 호소를 이용한 carotenoprotein의 추출조건 및 품질안정성에 대하여 검토하였다. 크릴가공 잔사중 총 astaxanthin함량은 자숙크릴 잔사 및 생동결 크릴 잔사에서 각각 $35.1mg\%,\;22.1mg\%$ 였으며, $98.6mg\%,\;61.9mg\%$였다. Chitin함량은 크릴가공 잔사인 자숙크릴과 생동결크릴에서 각각 $6.9\%,\;4.5\%$였으나 carotenoprotein에서는 검출되지 않았다. Trypsin이 carotenoprotein추출에 가장 효과적이었으며, papain, pepsin, protease는 거의 효과가 나타나지 않았다. Trypsin 첨가농도 $0.5\%$, 반응온도 $4^{\circ}C$에서 24시간 분해하였을 때 단백질 및 carotenoid의 회수율은 자숙시료가 각각 $83\%,\;74\%$로 가장 좋았다. 생동결크릴의 경우는 자숙크릴에 비해 단백질 및 carotenoid 회수율이 다소 떨어졌다. Carotenoprotein의 아미노산조성중 glutamic acid 와 aspartic acid의 함량이 가장 많았으며 필수 아미노산함량도 전체 아미노산의 $38.3\%\~43.6\%$를 차지하였다. Carotenoprotein중의 carotenoid는 TLC분석에 의해 astaxanthin, astaxanthin monoester, astaxanthin diester로 분리되었으며, 이들의 상대량은 각각 $25\~30\%\;,35\~40\%\;40\~45\%$였다. Carotenoprotein의 안정성은 저온($-20^{\circ}C\;4^{\circ}C$)일수록 안정하였으며, BHT와 protease inhibitor인 trasylol을 동시에 가한 것이 BHT와 trasylol만을 단독으로 가한 것보다 안정성이 좋았다.

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일제강점기 '전위미술론'의 전통관 연구 - '문장(文章)' 그룹을 중심으로 (A Study on Avant-Garde Fine Art during the period of Japanese Colonial Rule of Korea, centering on 'Munjang' (a literary magazine))

  • 박계리
    • 미술이론과 현장
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    • 제4호
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    • pp.57-76
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    • 2006
  • From the late 1920s to the 1930s, Korea's fine art community focused on traditional viewpoints as their main topic. The traditional viewpoints were discussed mainly by Korean students studying in Japan, especially oil painters. Such discussions on tradition can be divided into two separate halves, namely the pre- and post-Sino-Japanese War (1937) periods. Before the war, the modernists among Korea's fine art community tried to gain a fuller understanding of contemporary Western modern art, namely, expressionism, futurism, surrealism, and so forth, on the basis of Orientalism, and borrow from these schools' in order to create their own works. Furthermore, proponents of Joseon's avant-garde fine arts and artists of the pro-fine art school triggered debate on the traditional viewpoints. After the Sino-Japanese War, these artists continued to embrace Western modern art on the basis of Orientalism. However, since Western modern fine art was regressing into Oriental fine art during this period, Korean artists did not need to research Western modern fine art, but sought to study Joseon's classics and create Joseon's own avant- garde fine art in a movement led by the Munjang group. This research reviews the traditional view espoused by the Munjang group, which represented the avant-garde fine art movement of the post-war period. Advocating Joseon's own current of avant-garde fine art through the Munjang literary magazine, Gil Jin - seop, Kim Yong-jun and others accepted the Japanese fine art community's methodology for the restoration of classicism, but refused Orientalism as an ideology, and attempted to renew their perception of Joseon tradition. The advocation of the restoration of classicism by Gil Jin-seop and Kim Yong-jun appears to be similar to that of the Yasuda Yojuro-style restoration of classicism. However, Gil Jin-seop and Kim Yong-jun did not seek their sources of classicism from the Three-Kingdoms and Unified Silla periods, which Japan had promoted as a symbol of unity among the Joseon people; instead they sought classicism from the Joseon fine art which the Japanese had criticized as a hotbed of decadence. It was the Joseon period that the Munjang group chose as classicism when Japan was upholding Fascism as a contemporary extremism, and when Hangeul (Korean writing system) was banned from schools. The group highly evaluated literature written in the style of women, especially women's writings on the royal court, as represented by Hanjungnok (A Story of Sorrowful Days). In the area of fine art, the group renewed the evaluation of not only literary paintings, but also of the authentic landscape paintings refused by, and the values of the Chusa school criticized as decadent by, the colonial bureaucratic artists, there by making great progress in promoting the traditional viewpoint. Kim Yong-jun embraced a painting philosophy based on the painting techniques of Sasaeng (sketching), because he paid keen attention to the tradition of literary paintings, authentic landscape paintings and genre paintings. The literary painting theory of the 20th century, which was highly developed, could naturally shed both the colonial historical viewpoint which regarded Joseon fine art as heteronomical, and the traditional viewpoint which regarded Joseon fine art as decadent. As such, the Munjang group was able to embrace the Joseon period as the source of classicism amid the prevalent colonial historical viewpoint, presumably as it had accumulated first-hand experience in appreciating curios of paintings and calligraphic works, instead of taking a logical approach. Kim Yong-jun, in his fine art theory, defined artistic forms as the expression of mind, and noted that such an artistic mind could be attained by the appreciation of nature and life. This is because, for the Munjang group, the experience of appreciating nature and life begins with the appreciation of curios of paintings and calligraphic works. Furthermore, for the members of the Munjang group, who were purists who valued artistic style, the concept of individuality presumably was an engine that protected them from falling into the then totalitarian world view represented by the Nishita philosophy. Such a 20th century literary painting theory espoused by the Munjang group concurred with the contemporary traditional viewpoint spearheaded by Oh Se-chang in the 1910s. This theory had a great influence on South and North Korea's fine art theories and circles through the Fine Art College of Seoul National University and Pyongyang Fine Art School in the wake of Korea's liberation. In this sense, the significance of the theory should be re-evaluated.

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누리장나무 잎 추출물의 항산화 및 항염증 활성 (Anti-oxidant and Anti-inflammatory Properties of Clerodendrum trichotomum Leaf Extracts)

  • 김지혜;송하나;고희철;이주엽;장미경;김세재
    • 생명과학회지
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    • 제27권6호
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    • pp.640-645
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    • 2017
  • 누리장나무의 잎과 줄기는 예로부터 항고혈압, 관절염, 류마티스 관절염, 항염증 등을 치료하는 민간약재로 사용되어 왔다. 본 연구에서는 누리장나무의 잎 추출물과 그 분획물의 항산화 및 항염증 소재로서의 활성가능성을 확인하기 위하여 수행되었다. 누리장나무 잎의 ethanol (EtOH)로 추출물과 이 추출물을 분획하여 얻은 hexane, chloroform ($CHCl_3$), ethyl acetate (EtOAc), n-butanol (BuOH) 분획물들의 총 폴리페놀 함량을 비교한 결과, 총 폴리페놀 함량은 EtOAc 분획물($78.08{\mu}g/mg$)과 BuOH 분획물($77.54{\mu}g/mg$)에서 높게 측정되었다. 각 분획물의 항산화 활성을 비교한 결과, 폴리페놀 함량이 높은 EtOAc 분획물과 BuOH 분획물에서 높은 DPPH (1,1-diphenyl-2-picrylhydrazyl) 및 ABTS (2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid)) radical 소거활성을 나타내었다. 그리고 xanthine oxidase 저해활성은 $CHCl_3$ 분획물($IC_{50}=4.43{\mu}g/ml$)과 EtOAc 분획물($IC_{50}=5.69{\mu}g/ml$)에서 높게 나타났다. EtOAc 분획물은 RAW 264.7 세포에서 lipopolysaccharide (LPS)로 유도된 nitric oxide (NO)의 생성을 효과적으로 억제하였다($IC_{50}=18.87{\mu}g/ml$). 또한, EtOAc 분획물($100{\mu}g/ml$)은 LPS로 지극한 RAW 264.7 세포에서 tumor necrosis factor ${\alpha}$ ($TNF-{\alpha}$)와 interleukin-6 (IL-6)의 생성과 inducible nitric oxide synthase (iNOS)와 cyclooxygenase-2 (COX-2) 단백질의 발현을 효과적으로 억제하였다. 본 연구결과는 누리장나무 잎 추출물과 분획물이 항산화 및 항염증 소재로서의 활용가능성이 있음을 제시해 준다.

창업지향성과 학습지향성이 중소창업기업의 성과에 미치는 영향에 관한 연구 (The Study on The Effect of Entrepreneurial Orientation and Learning Orientation Toward to SME's Performance)

  • 조세근;손종서;이우진
    • 벤처창업연구
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    • 제10권6호
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    • pp.1-13
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    • 2015
  • 중소창업기업이 급변하는 시장에서 살아남기 위해서는 다양한 성과요인들이 있으며, 많은 연구결과에서 창업자의 창업지향성 확립이 중요하다고 논의되고 있다. 따라서 중소창업기업이 지속적인 경쟁우위를 확보하고 새로운 기회를 포착하기 위한 요인에 대한 분석과 기업성과 간의 관계 분석은 많은 중소창업기업에게 방향을 제시하며, 가치 있는 일이라 할 수 있겠다. 본 연구에서는 창업지향성(Entrepreneurial Orientation)의 주요 변수에 대한 탐색적 연구를 통해 기업성과(Firm Performance)에 대한 영향 요인을 고찰하고 실증적 연구를 통하여 확인 하였다. 본 연구는 중소창업기업 CEO의 창업지향성, 학습지향성(Learning Orientation)과 기업성과 간의 관련성을 탐색하기 위한 연구로 진행되었으며 다음과 같은 부분을 검증하였다. 첫째, 창업지향성(진취성, 경쟁적 공격성, 위험감수성, 혁신성)이 학습지향성에 미치는 영향을 살펴보고자 하였으며, 둘째, 창업지향성이 기업성과에 미치는 영향을 살펴보고자 하였으며, 마지막으로 학습지향성이 창업지향성과 기업성과 간에 매개되는 영향요인을 실증적 연구를 통해 검증하였다. 본 연구는 학습지향성이 창업자의 창업지향성과 기업성과 간의 관계에 있어서 매개효과를 밝혔다는 점에서 이론적 기여하며, 창업지향성의 개념 정의에 있어서 국내에서 연구된 기존 선행연구와는 다른 새로운 차원으로 정의하여 연구를 수행하였다. 본 연구 결과, 각각의 중소창업기업은 다양한 창업지향성을 바탕으로 기업성과에 상이한 영향력을 가지고 있음을 보여주었다. 이를 통하여 창업지향성에 대한 독립적인 연구의 필요성을 제시하고 있다. 결론적으로 중소창업기업들에게 있어 창업지향성은 기업성과에 매우 중요한 역할을 하고 있다는 점과 중소창업기업의 창업 경영자는 경쟁적 공격성보다는 혁신적이며, 위험 감수적인 의사결정이 기업 활동에 보다 긍정적인 영향을 미친다는 것을 시사점으로 제시하였다. 또한, 위험감수성은 정성적 기업성과에 긍정적이며, 혁신성은 정량적 경영성과와 정성적 기업성과 모두에 긍정적 영향을 미친다는 결론을 도출하였다. 이러한 본 연구의 결론은 중소창업기업 창업 경영자들이 창업지향성을 수립 시, 필요한 실무적인 기초 자료로 활용 가능하리라 기대한다.

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아세트아미노펜 유도 HepG-2 세포주 손상에 대한 굴 효소 가수분해물의 보호 효과 (Protective Effects of Enzymatic Oyster Hydrolysate on Acetaminophen-induced HepG-2 Cell Damage)

  • 박시향;문성실;;정세영;최영준
    • 한국식품영양과학회지
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    • 제43권8호
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    • pp.1166-1173
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    • 2014
  • 본 연구는 굴 가수분해물이 아세트아미노펜에 의한 간독성의 무독화 효과를 HepG-2 세포를 사용하여 조사하였다. 굴 가수분해물은 굴 단백질의 가교연결을 위해 TGase로 전처리하거나(TGPN) 혹은 하지 않고(PN), 1% Protamex와 1% Neutrase 단백질 분해효소로 2단 가수분해하였다. 두 종류의 굴 가수분해물은 아세트아미노펜으로 간 손상을 유도한 세포에 각각 처리하여 세포 생존율을 측정하였으며, 세포 배양 시 배양액으로 유출된 GOT와 GPT 활성을 측정하였다. TGPN 가수분해물의 경우 아세트아미노펜만을 처리한 negative 대조군($60.7{\pm}3.2%$)에 비하여 $100{\mu}g/mL$$200{\mu}g/mL$의 농도에서 각각 $136.2{\pm}1.4%$$179.6{\pm}3.8%$의 높은 세포 생존율을 보였다. PN 가수분해물은 $100{\mu}g/mL$$200{\mu}g/mL$의 농도에서 각각 $107.9{\pm}8.8%$$130.6{\pm}7.6%$의 세포 생존율을 보였다. GOT 활성은 negative 대조군의 경우에 $38.3{\pm}0.2$ Karmen/mL이었으며, TGPN($200{\mu}g/mL$)과 PN($200{\mu}g/mL$)에서는 $19.9{\pm}0.5$$22.0{\pm}2.4$ Karmen/mL로 농도에 따라 유의적인 활성 감소를 확인할 수 있었다. 그리고 GPT 활성도 GOT와 같은 경향의 활성을 나타내었다. 이 같은 결과에 미루어 굴 유래 가수분해물의 간 보호 건강 기능 식품 혹은 약물 개발의 가능성을 확인하였으며 앞으로 가수분해 펩티드 중의 유효성분에 대한 구조동정과 작용 기전에 관한 연구가 필요할 것으로 보인다.

차아염소산나트륨과 클로르헥시딘의 반응침전물 형성방지를 위한 여러 가지 근관세척 방법의 비교 (THE COMPARISON OF DIFFERENT CANAL IRRIGATION METHODS TO PREVENT REACTION PRECIPITATE BETWEEN SODIUM HYPOCHLORITE AND CHLORHEXIDINE)

  • 최문선;박세희;조경모;김진우
    • Restorative Dentistry and Endodontics
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    • 제35권2호
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    • pp.80-87
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    • 2010
  • 이 실험의 목적은 NaOCl과 CHX의 혼합사용 시, 발생하는 침전물의 형성을 막기 위해 두 용액간의 접촉을 줄일 수 있는 여러 가지 방법을 비교하고, 관찰된 침전물의 원소를 분석하는 것이다. 발거된 50개의 단근치를 사용하였으며 2.5% NaOCl을 이용하여 .04 taper ProFile #40까지 근관형성 하였다. 치아는 다음과 같은 근관세척 방법에 따라 4개의 실험군과 1개의 대조군으로 나누었다; 대조군: 2.5% NaOCl, 1군: 2.5% NaOCl + 2% CHX, 2군: 2.5% NaOCl + paper points + 2% CHX, 3군: 2.5% NaOCl + .04/#45 근관확대 + 2% CHX, 4군: 2.5% NaOCl +95% alcohol+ 2% CHX. 근관세척 후 치아를 양분하고 치관부, 중간부, 치근부 세부위로 나누어 전계 방사형 주사 전자현미경을 통하여 잔사 비율, 개방 상아세관 비율, 상아세관 내 물질의 원소분석을 시행하였다. 실험결과, 실험군 사이에 잔사비율과 개방된 상아세관 비율 비교에서 통계학 적으로 유의할 만한 차이는 나타나지 않았다. 1 군의 한 시편에서 C의 함유량이 높게 나타났으며 N과 Cl도 함께 검출되어 para-chloraniline으로 추정되며, 1 군의 다른 시편과 나머지 실험군에서는 O, P, C, Ca의 순으로 함유량이 높은 것으로 나타나 수산화인회석으로 추정된다. NaOCl 세척 후 바로 CHX 세척을 한 군에서 PCA로 의심되는 물질이 검출된 바, 두 용액의 직접적인 접촉을 피하기 위해 주의가 필요하며, 본 실험에 사용된 여러 근관 세척방법 이용 시 침전물의 형성을 예방할 수 있을 것이다.