• Asian-Australasian Journal of Animal Sciences
• /
• v.25 no.9
• /
• pp.1205-1215
• /
• 2012

This study was conducted to establish genetic criteria for phenotypic characteristics of Hanwoo cattle based on allele frequencies and genetic variance analysis using microsatellite markers. Analysis of the genetic diversity among 399 Hanwoo cattle classified according to nose pigmentation and coat color was carried out using 22 microsatellite markers. The results revealed that the INRA035 locus was associated with the highest $F_{is}$ (0.536). Given that the $F_{is}$ value for the Hanwoo INRA035 population ranged from 0.533 (white) to 1.000 (white spotted), this finding was consistent with the loci being fixed in Hanwoo cattle. Expected heterozygosities of the Hanwoo groups classified by coat colors and degree of nose pigmentation ranged from $0.689{\pm}0.023$ (Holstein) to $0.743{\pm}0.021$ (nose pigmentation level of d). Normal Hanwoo and animals with a mixed white coat showed the closest relationship because the lowest $D_A$ value was observed between these groups. However, a pair-wise differentiation test of $F_{st}$ showed no significant difference among the Hanwoo groups classified by coat color and degree of nose pigmentation (p<0.01). Moreover, results of the neighbor-joining tree based on a $D_A$ genetic distance matrix within 399 Hanwoo individuals and principal component analyses confirmed that different groups of cattle with mixed coat color and nose pigmentation formed other specific groups representing Hanwoo genetic and phenotypic characteristics. The results of this study support a relaxation of policies regulating bull selection or animal registration in an effort to minimize financial loss, and could provide basic information that can be used for establishing criteria to classify Hanwoo phenotypes.

• Korean Journal of Ichthyology
• /
• v.17 no.4
• /
• pp.279-286
• /
• 2005

The fish species composition and its seasonal variation of the middle East Sea of Korea were investigated from May 2004 to January 2005 using gill and set nets. A total of 59 species of fishes belonging to 31 families in 11 orders were identified in the gill and set nets; 33 species in 15 families and 6 orders were collected by the gill net, and 36 species in 26 families and 10 orders were collected by the set net. Among the fishes collected by gill net, the order Scorpaeniformes was dominant; the number of species as well as individuals was highest in summer; the biomass was highest in winter. However, in fishes collected by set net, the order Perciformes was dominant, the number of species was highest in autumn, the number of individuals was highest in spring, and the biomass was highest in winter. The fish composition of the middle East Sea differed considerably from that of the south East Sea, especially in the species composition of the order Scorpaeniformes and Pleuronectiformes. Principal component analyses for each net showed that the fish species composition depends on the kind of net used, even though the investigation was carried out in the same area and at the same time.

• Toxicological Research
• /
• v.23 no.1
• /
• pp.55-63
• /
• 2007

Diethylnitrosamine (DEN) is a nitrosamine compound that can induce a variety of liver lesions including hepatic carcinoma, forming DNA-carcinogen adducts. In the present study, microarray analyses were performed with Affymetrix Murine Genome 430A Array in order to identify the gene-expression profiles for DEN and to provide valuable information for the evaluation of potential hepatotoxicity. C57BL/6NCrj mice were orally administered once with DEN at doses of 0, 3, 7 and 20 mg/kg. Liver from each animal was removed 2, 4, 8 and 24 hrs after the administration. The histopathological analysis and serum biochemical analysis showed no significant difference in DEN-treated groups compared to control group. Conversely, the principal component analysis (PCA) profiles demonstrated that a specific normal gene expression profile in control groups differed clearly from the expression profiles of DEN-treated groups. Within groups, a little variance was found between individuals. Student's t-test on the results obtained from triplicate hybridizations was performed to identify those genes with statistically significant changes in the expression. Statistical analysis revealed that 11 genes were significantly downregulated and 28 genes were upregulated in all three animals after 2 h treatment at 20 mg/kg. The upregulated group included genes encoding Gdf15, JunD1, and Mdm2, while the genes including Sox6, Shmt2, and SIc6a6 were largely down regulated. Hierarchical clustering of gene expression also allowed the identification of functionally related clusters that encode proteins related to metabolism, and MAPK signaling pathway. Taken together, this study suggests that match with a toxicant signature can assign a putative mechanism of action to the test compound if is established a database containing response patterns to various toxic compounds.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
• /
• 2001.06a
• /
• pp.2111-2111
• /
• 2001

A chemoinfometrical method for evaluating the quantitative determination of crystallinity one polymorphs based on fourie-transformed near-infrared (FT-NIR) spectroscopy was established. A direct comparison of the data with the ones collected from using the and compared with the conventional powder X-ray diffraction method was performed. [Method] The pPure a and g forms of indomethacin (IMC) were prepared by reportedusing published methods. Six kinds of standard samples obtained by physically mixing of a and g forms. After the powder X-ray diffraction profiles of samples have been measured, the intensity values were normalized to against the intensity of silicon powder as the as an external standard. The calibration curves for quantification of crystal content were based upon the total relative intensity of four diffraction peaks from of the form g crystal. FT-NIR spectra of six calibration sample sets were recorded 5 times with the NIR spectrometer (BRAN+LUEBBE). Chemoinfometric analysis was performed on the NIR spectral data sets by applying the principal component regression (PCR). [Results] The relation between the actual and predicted polymorphic contents of form g IMC measured using by the X-ray diffraction method shows a good straight linen linear relation., and it has slope of 0.023, an intercept of 0.131 and a correlation coefficient of 0.986. PCR analyses wereis was performed based on normalized NIR spectra sets offer standard samples of known content of IMC g form. IMC. A calibration equation was determined to minimize the root mean square error of the predictionthe prediction. Figure 1 shows a plot of the calibration data obtained by NIR method between the actual and predicted contents of form g IMC. The predicted values were reproducible and had a smaller standard deviation. Figure 2 shows that the plot for the predicted transformation rate (%) of form a IMC to form g as measured by X-ray diffractomeoy against to those as measured by NIR method. The plot has a slope of 1.296, an intercept of 1,109, and a correlation coefficient of 0.992. The line represents a satisfactory correlation between the two predicted values of form g IMC content. Thus NIR spectroscopy is an effective method for the evaluation to the pharmaceutical products of quantitative of polymorph.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
• /
• 2001.06a
• /
• pp.1126-1126
• /
• 2001

The paper presented here is the initial part of a larger study, in which it was determined which quality parameters in cheese powder could already be predicted by NIR at an early stage in the process and which could only be predicted at the final stages of the process. This initial study was performed in order to establish the levels and nature of variation within and between batches such that the subsequent data collection could be tackled optimally. The perspectives evolved into more than was originally planned and revealed some interesting uses of NIR-technology. Cheese powder production starts as a batch process, where waste cheese from other dairies is melted down in a vat. The process then turns into a continual process as the vat is emptied and the melted cheese is then filtered, homogenized, pasteurized and finally spray dried. Between each batch the powder is to a greater or lesser degree a mixture of 2 batches. This paper is divided into 2 aspects, one regarding the optimization of sampling time and the other is a study of process dynamics. Optimizing sampling time This initial study included 9 powder samples from 9 different batches produced during one day. The raw materials for the batches were chosen with the aim of creating a relatively high level of variation in the data. The total of 81 samples were taken out at regular intervals and spectra were collected on a NIR-systems 6500 instrument. The subsequent reduction of the data by PCA to score values shows the power of NIR as a tool to determine not only when samples are representative of a certain batch, but also which batches are stable enough to include in a further study. Studying process dynamics To take this experiment a step further 1 of the 81 samples were sent to the laboratory for further analyses. The samples were chosen on the criteria that they covered the spectral variation in the dataset. These samples were analysed for 4 chemical components and 5 physical attributes, which are essential for describing the quality of the product. The latent structure of the 7 samples, using the chemical and physical variables, is totally comparable to the latent structure of the NIR spectra. This outcome makes it possible to describe the dynamics of one day's production both chemically and physically with relatively little resources. Additionally it raises the question as to whether reference values are needed, as the latent structure of the NIR-spectra appears to be sufficient in providing information on the quality of the product. To be able to use NIR in this way would require defining quality limits in the principal component space as opposed to each of the reference values. The potential of NIR applied in an explorative fashion with batch processes opens a whole new gateway for the use of this technology. This study explains yet again after so many years in the field “why I'm crazy about NIR!”.

• Women's Health Nursing
• /
• v.8 no.1
• /
• pp.106-115
• /
• 2002

Little is known about sexual autonomy in the light of human sexual health or sexual behavior. The purpose of this study was to increase understanding of sexual autonomy by developing a scale to measure sexual autonomy in college students. The measure was based on the perspective of human beings as having autonomy. A methodological developmental design was used. The study was done in three steps and included concept development through content analysis of relevant literature individual interviews using open-ended questions with 19 college students, item analysis of data from self report questionnaires for item to total correlation, content validity index by professionals and college students, and factor analysis with principal component analysis. Three groups participated in testing the measurement scale; Group A in 1998, a nationwide convenience sample of college students (n=1,163), Group B, college students in 1999 (n=233), and Group C, college students in 2000 who responded to the measurement scale before and after a sex education program (n=216). A series of exploratory and confirmatory factor analyses were conducted to test validity. For analysis of the questionnaires, statistical methods including reliability (test-retest), item to total analysis, content validity, factor analysis for construct validity and t-test for mean difference according to gender, sexual coitus, previous sex education, and pre-post experiment sex education were used with SPSS 10.0. Results: 1. Two stable factors were extracted and these contributed 48.05% of the variance in the total score. All 13 items loaded above .40 on each factor. 2. Factor I with 10 items was named 'control' and factor II with 3 items and was named 'coping'. 3. Differences in sexual autonomy scores for college students according to their experience showed that there were significantly higher scores for sexual autonomy in the post sex education group(t=-10.841, p=.000), but there were no differences according to whether or not they had previous sex education. Female college students had higher sexual autonomy scores than male college students in A or B group (t=-5.933, p=.000/ t=-1.992, p=.048). 4. Cronbach's $\alpha$ was .8603 for the total 13 items with 1,632 college students, .8739 with 232 students, .8333$\sim$.7647 with pre-post sex education with 216 students. This measurement was found to have a high validity and reliability for measuring sexual autonomy in college students. Therefore, the author recommends that it be used to measure the effects of sex education. It is suggested that further study is needed to analyze the relationship between sexual autonomy and sexual behavior.

• Korean Journal of Soil Science and Fertilizer
• /
• v.44 no.2
• /
• pp.242-247
• /
• 2011

Soil management for environment-friendly agriculture depends on the effects of soil microbial activities and soil fertility. To improve soil health for the upland crops, this study evaluated a relationship between soil chemical properties and soil microbial diversities at 25 sites in upland soils in Gyeongnam Province. The average nutrients in the upland soils were 1.7 times for available phosphorous, 1.4 times for exchangeable potassium and 1.5 times for exchangeable calcium higher compared to recommend concentrations in the upland soils. We found a significant positive correlation between the soil organic matter and the soil microbial biomass C (p<0.01). Contents of organic matter and dehydrogenase in the inclined piedmont soils were significantly higher than those in the other topographical soils (p<0.05). In addition, concentrations of organic matter and microbial biomass C in the loam soils were significantly higher than in the silt loam soils (p<0.05). In principal component analyses of chemical properties and microbial populations in the upland soils, our findings suggested that available phosphorous should be considered as potential factor responsible for the clear upland soils differentiation. The soil organic matter was positive correlation with Bacillus sp. and fungi, whereas soil pH was also positive correlation with Pseudomonas sp. in upland soils.

• Korean Journal of Social Welfare
• /
• v.59 no.2
• /
• pp.65-88
• /
• 2007

This study evaluates psychometric properties of the Revised Memory and Behavioral Problems Checklist(RMBPC) in Korean version. The sample includes 387 South Korean family caregivers who provide the majority of day to day tasks and emotional care for their older family members with physical and/or cognitive disabilities. The psychometric properties of the RMBPC are verified by item analysis, exploratory factor analysis(EFA) & confirmatory factor analysis(CFA). For cross-validity, the randomly divided two samples are used: one sample for EFA(n=193, 387/2) and the other sample for CFA(n=194, 387/2). The internal consistency of the K-RMBPC is excellent, and the convergent and criterion-related validity of the K-RMBPC with related variables is empirically confirmed. An EFA based on the Principal Component Analysis(PCA) method with varimax rotation explains 65.85% of variance accounted for by the three factors. A CFA also verifies that the model fit was acceptable. The shorter Korean version of the RMBPC is found reliable and valid. The translated checklist would be very useful for clinical and research settings by (a) focusing on observable, conceptually relevant, potentially modifiable behaviors and (b) using objective criteria within a self-administered framework, to enable clinicians and researchers to pinpoint areas of disturbance and target intervention goals for patients and caregivers in a cost-effective manner.

• Asian-Australasian Journal of Animal Sciences
• /
• v.26 no.12
• /
• pp.1762-1772
• /
• 2013

The objective of the current study was to determine the effect of pre-slaughter stress, season and breed on the activity of plasma creatine kinase (CK) and the quality of mutton. One hundred and seventy-three (173) castrated sheep from Dormer (DM), South African Mutton Merino (SAMM), Dorper (DP) and Blackhead Persian (BP) sheep breeds were used in the study. The animals were grouped according to age-groups as follows: Group 1 (6 to 8 months), Group 2 (9 to 12 months) and Group 3 (13 to 16 months). Blood samples were collected during exsanguinations using disposable vacutainer tubes for CK analysis. Representative samples of the Muscularis longissimuss thoracis et. lumborum (LTL) were collected from 84 castrated sheep, of different breeds (28 per breed) 24 h after slaughter. The following physico-chemical characteristics of mutton were determined; meat pH ($pH_{24}$), color ($L^*$, $a^*$ and $b^*$), thawing and cooking losses and Warner Braztler Shear Force (WBSF). The activity of plasma CK was significantly higher (p<0.001) in summer ($1,026.3{\pm}105.06$) and lower in winter ($723.3{\pm}77.75$). There were higher values for $L^*$ ($33.7{\pm}0.94$), $b^*$ ($11.5{\pm}0.48$) and WBSF ($29.5{\pm}1.46$) in summer season than in winter season; $L^*$ ($29.4{\pm}0.64$), $b^*$ ($10.2{\pm}0.33$) and WBSF ($21.2{\pm}0.99$). The activity of plasma CK was influenced by the type of breed with Dormer having the highest (p>0.001) levels ($1,358.6{\pm}191.08$) of CK. South African Mutton Merino had higher values for $pH_{24}$ ($5.9{\pm}0.06$), $L^*$ ($34.2{\pm}0.97$), $b^*$ ($12.2{\pm}0.50$) and WBSF ($26.8{\pm}1.51$) and Blackhead Persian had higher values ($35.5{\pm}2.17$) for cooking loss (CL%) than the other breeds. Computed Principal Component Analyses (PCA) on the activity of plasma CK and physico-chemical characteristics of mutton revealed no correlations between these variables. However, positive correlations were observed between $pH_{24}$, $L^*$, $a^*$, $b^*$, CL% and WBSF. Relationships between pre-slaughter stress, CK activity and physico-chemical characteristics of mutton were also observed. It was therefore concluded that although mutton quality and creatine kinase were not related, pre-slaughter stress, season and breed affected the activity of creatine kinase and mutton quality.

• Science of Emotion and Sensibility
• /
• v.12 no.1
• /
• pp.121-128
• /
• 2009

The article reports findings on: (1) development of emotion assessment scale in evaluating the Television(TV) picture quality; and (2) how psychological and physical factors relate to TV picture quality. A total of 152 adjectives that specifically describe emotional reactions were first selected from a Korean dictionary of adjectives, followed by ratings on their suitability for the evaluation of TV picture quality. The final selection of 19 adjective, based on the reported rating scores greater than 4.1, were used on 126 college students who were asked to perform similarity ratings on the adjectives. Based on factor analyses (i.e., principal component analysis with oblique rotation) on the similarity of scores, the following adjectives were selectively chosen for the development of the new emotion assessment scale: 'neat-messy', 'refreshing-gloomy', 'clean-dirty', 'comfortable-tense', 'smooth-rough', 'bright-dark', 'gorgeous-plain', 'diverse-monotonous', 'satisfying', 'natural', and 'sensuous'. These adjectives composed into two distinct constructs, 'cleanness or smart' factor and 'gorgeousness' factor, which demonstrated sensitivity to changes in brightness, contrast, color, and tint in the TV picture quality, except for changes in sharpness.