• Title/Summary/Keyword: Principal Components

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The Stereospecific Analysis of the Triacylglycerols of Ginkgo Nut Oils by High-Performace Liquid Chromatography(HPLC) in the Silver Ion and Reversed Phase Modes (銀Ion 및 역상(逆相) HPLC를 이용(利用)한 입체이성체적(立體異性體的) 분석(分析)에 의한 은행종실유(銀杏種實油)의 트리글리세리드의 분자종(分子種)에 관한 연구(硏究))

  • Joh, Yong-Goe;Woo, Hyo-Kyeng;Kim, Hoon-Sook
    • Journal of the Korean Applied Science and Technology
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    • v.14 no.1
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    • pp.61-76
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    • 1997
  • Triacylglycerols of the seeds of Ginkgo biloba have been resolved by high-performace liquid chromatography(HPLC} in the silver-ion and reverse-phase modes. The fatty acids were identified by a combination of capillary gas chromatography and gas-chromatography /mass spectrometry as the methyl and /or picolinyl ester. The main components are $C_{18:2{\omega}6}$(39.0mol%), $C_{18:1{\omega}7}$(asclepic acid 21.5mol%), and $C_{18:1{\omega}9}$(oleic acid, 13.8mol%). Considerable amounts of unusual acid such as $C_{20:3{\Delta}^{5,11,14}$ (5.7mol%), $C_{18:2{\Delta}^{5,9}$(2.8mol%), and $C_{18:3}{\Delta}^{5,9,12}$(1.6mol%), were checked. In addition, an anteiso-branched fatty acid, 14-methylhexadecanoic acid, was also present as a minor component(0.9 mol%). The triacylglycerols were separated into 17 fractions by reverse-phase HPLC, and the fractionation was achieved according to the partition numnber(PN) in which a ${\Delta}^5$-non methylene interrupted double bond($^5$-NMDB) showed different behaviour from a methylene interrupted double bond in a molecule with a given cahinlength. Silver-ion HPLC exhibited excellent resolution in which fractions(23 fractions) were resolved on the basis of the number and configuration of double bonds. In this instance, the strength of interaction of a ${\Delta}^5$-NMDB system with silver ions seemed to be weaker than a methylene interrupted double bond system. The principal triacylglycerol species are as follows ; $(C_{18:2{\omega}6)2}/C_{18:1{\omega}7}$, $C_{18:1{\omega}9}/C_{18:1{\omega}7}/C_{18:2{\omega}6}$, $(C_{18:1{\omega}7)2}/C_{18:2{\omega}6}$, $C_{16:1{\omega}7}/C_{18:1{\omega}9}/C_{20:3}{\Delta}^{5,11,14}$, $C_{16:1{\omega}7}/C_{18:1{\omega}7}/C_{20:3}{\Delta}^{5,11,14}$, $C_{18:1{\omega}9}/C_{18:1{\omega}7}/C_{18:2{\omega}6}$, $C_{18:1{\omega}9}/C_{18:2}{\Delta}^{5,5}/C_{20:3}{\Delta}^{5,11,14}$, $(C_{18:1{\omega}7)2}/C_{18:2{\omega}6}$ and $(C_{18:1{\omega}9)2}/C_{18:2{\omega}6}$, while simple triacylglycerols without $C_{18:2{\omega}6})_3$ were not present. Stereospecific analysis showed that fatty acids with ${\Delta}^5$-NMDB system and saturated chains were predominantly located at the site of sn-3 carbon of glycerol backbones. It is evident that there is asymmetry in the distribution of fatty acids in the TG molecules of Ginkgo nut oils.

Effects of the Feeding Mixed Oils with Various Level of n-3 and n-6 Polyunsaturated Fatty Acid on the Lipid Components of Liver, Brain, Testis and Kidney in Dietary Hyperlipidemic Rats (n-3 및 n-6계 다불포화 지방산의 함유비율이 다른 유지가 식이성 고지혈증 흰쥐의 간장, 뇌, 고환 및 신장의 지질 성분에 미치는 영향)

  • 김한수;김성희;김군자;최운정;정승용
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.22 no.6
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    • pp.685-691
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    • 1993
  • This study was designed to examine effects of the various levels of sardine and safflower oil mix on lipid contents of serveral tissues in dietary hyperlipidemic rats. Experimental oils were 16% butter(control group), 8% butter +8% olive oil(group 2) 8% butter+8% sardine oil(group 3) 8% butter+6% sardine oil+2% safflower oil(group 4), 8% butter+4% sardine oil+4% safflower oil(group 5),8% butter+2% sardine oil+6% safflower oil(group 6) and 8% butter+8% safflower oil(group 7). The diet administered to the male rats of Sprague-Dawley were fed for 4 weeks. In livers, total cholesterol and triglyceride, phospholipid concentrations were lowest in the group 5 and free cholesterol concentrations were lower in the groups 4 and 5, particularly. Total cholesterol and triglyceride concentrations in brain were significantly lower in the group 5 and phospholipids were lowest in the group 3, while free cholesterol were group 7. In testes, total cholesterol and triglyceride, phospholipid, free cholesterol concentrations were lower in the all experimental groups than the control group, but those of kidney were lower in the groups 3, 7 than in the control group. Feeding mixed oil having equal quantity of sardine oil and safflower oil were effective on the reduction of the lipid contents in the principal tissues. It might be due to the effects of appropriate ratios of P/S, 0.85 and n-6/n3-p, 2.85 in the test lipids.

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Carbohydrate and lipid spectroscopic molecular structures of different alfalfa hay and their relationship with nutrient availability in ruminants

  • Yari, Mojtaba;Valizadeh, Reza;Nnaserian, Abbas Ali;Jonker, Arjan;Yu, Peiqiang
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.11
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    • pp.1575-1589
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    • 2017
  • Objective: This study was conducted to determine molecular structures related to carbohydrates and lipid in alfalfa hay cut at early bud, late bud and early flower and in the afternoon and next morning using Fourier transform infrared spectroscopy (FT/IR) and to determine their relationship with alfalfa hay nutrient profile and availability in ruminants. Methods: Chemical composition analysis, carbohydrate fractionation, in situ ruminal degradability, and DVE/OEB model were used to measure nutrient profile and availability of alfalfa hay. Univariate analysis, hierarchical cluster analysis (CLA) and principal components analysis (PCA) were conducted to identify FT/IR spectra differences. Results: The FT/IR non-structural carbohydrate (NSCHO) to total carbohydrates and NSCHO to structural carbohydrate ratios decreased (p<0.05), while lignin to NSCHO and lipid CH3 symmetric to CH2 symmetric ratios increased with advancing maturity (p<0.05). The FT/IR spectra related to structural carbohydrates, lignin and lipids were distinguished for alfalfa hay at three maturities by PCA and CLA, while FT/IR molecular structures related to carbohydrates and lipids were similar between alfalfa hay cut in the morning and afternoon when analyzed by PCA and CLA analysis. Positive correlations were found for FT/IR NSCHO to total carbohydrate and NSCHO to structural carbohydrate ratios with non-fiber carbohydrate (by wet chemistry), ruminal fast and intermediately degradable carbohydrate fractions and total ruminal degradability of carbohydrates and predicted intestinal nutrient availability in dairy cows ($r{\geq}0.60$; p<0.05) whereas FT/IR lignin to NSCHO and CH3 to CH2 symmetric stretching ratio had negative correlation with predicted ruminal and intestinal nutrient availability of alfalfa hay in dairy cows ($r{\geq}-0.60$; p<0.05). Conclusion: FT/IR carbohydrate and lipid molecular structures in alfalfa hay changed with advancing maturity from early bud to early flower, but not during the day, and these molecular structures correlated with predicted nutrient supply of alfalfa hay in ruminants.

Classification of Body Types for sizes of Ready-to-Wear-focusing on Korean female aged from 18 to 24 (성인 여성의 기성복 치수를 위한 체형 분류)

  • 김경화;남윤자
    • Journal of the Korean Society of Costume
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    • v.53 no.6
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    • pp.145-159
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    • 2003
  • The purpose of this study was to classify body type for ready-to-wear sizes. The subjects were 300 women ages of 18-24. they were measured direct anthropometry. The body types for sizing system were divided by Rohrer Index. KS drop value and ISO drop value. The results of this study were as follows. 1. By adapting the Rohrer Index. we classify 3 types from anthropometric measurements. The thin type covered 39.3%, the standard type 51.0% and the obesity type 18.7%. The characteristics of clusters were as follows. Thin type was characterized by tall. slender type and slim. The standard type was characterized by middle sized. The obesity type was characterized by short. fat type. and large bust. 2. By adapting the KS system drop value. we classify 3 types from anthropometric measurements. The H type(drop 0) covered 25.6%. the N type(drop 6) 65.2% and the A type(drop 12) 9.2%. Type H was slightly tall large bust. and curved from waist to hip. Type A was slightly thin. large hip and smaller bust than type N. Principal factor components were bust size. The height could be divided into three groups. The Petite(l50cm) covered 5.5%. the Regular(l60cm) 64.7% and the Tall(l70cm) 29.8%. Through the crosstab of height and body type. we extracted regular height by N type 46.2% the largest cell. The body type was the higher order of N type. H type and A type. The tall was the higher order of Regular. Tall and Petite. 3. By adapting the ISO system drop value. we classify 3 types from anthropometric measurements. The H type(drop 0) covered 15.0%. the M type(drop 6) 41.0% and the A type(drop 12) 44.0%. Type H was slightly short. slightly fat and large bust. Type A was slightly tall. slight thin than type M. The height could be divided into three groups. We adjust the height section after allow for height distribution. The Short(152cm) covered 12.8%. the Regular(160cm) 66.9% and the Long(168cm) 20.3%. Through the crosstab of height and body type, we extracted regular height by M type 29.3% the largest cell. The body type was the higher order of M type, A type and H type. The tall was the higher order of Regular, Long and short.

High Resolution Time Resolved Contrast Enhanced MR Angiography Using k-t FOCUSS (k-t FOCUSS 알고리듬을 이용한 고분해능 4-D MR 혈관 조영 영상 기법)

  • Jung, Hong;Kim, Eung-Yeop;Ye, Jong-Chul
    • Investigative Magnetic Resonance Imaging
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    • v.14 no.1
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    • pp.10-20
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    • 2010
  • Purpose : Recently, the Recon Challenge at the 2009 ISMRM workshop on Data Sampling and Image Reconstruction at Sedona, Arizona was held to evaluate feasibility of highly accelerated acquisition of time resolved contrast enhanced MR angiography. This paper provides the step-by-step description of the winning results of k-t FOCUSS in this competition. Materials and Methods : In previous works, we proved that k-t FOCUSS algorithm successfully solves the compressed sensing problem even for less sparse cardiac cine applications. Therefore, using k-t FOCUSS, very accurate time resolved contrast enhanced MR angiography can be reconstructed. Accelerated radial trajectory data were synthetized from X-ray cerebral angiography images and provided by the organizing committee, and radiologists double blindly evaluated each reconstruction result with respect to the ground-truth data. Results : The reconstructed results at various acceleration factors demonstrate that each components of compressed sensing, such as sparsifying transform and incoherent sampling patterns, etc can have profound effects on the final reconstruction results. Conclusion : From reconstructed results, we see that the compressed sensing dynamic MR imaging algorithm, k-t FOCUSS enables high resolution time resolved contrast enhanced MR angiography.

Region Based Image Similarity Search using Multi-point Relevance Feedback (다중점 적합성 피드백방법을 이용한 영역기반 이미지 유사성 검색)

  • Kim, Deok-Hwan;Lee, Ju-Hong;Song, Jae-Won
    • The KIPS Transactions:PartD
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    • v.13D no.7 s.110
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    • pp.857-866
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    • 2006
  • Performance of an image retrieval system is usually very low because of the semantic gap between the low level feature and the high level concept in a query image. Semantically relevant images may exhibit very different visual characteristics, and may be scattered in several clusters. In this paper, we propose a content based image rertrieval approach which combines region based image retrieval and a new relevance feedback method using adaptive clustering together. Our main goal is finding semantically related clusters to narrow down the semantic gap. Our method consists of region based clustering processes and cluster-merging process. All segmented regions of relevant images are organized into semantically related hierarchical clusters, and clusters are merged by finding the number of the latent clusters. This method, in the cluster-merging process, applies r: using v principal components instead of classical Hotelling's $T_v^2$ [1] to find the unknown number of clusters and resolve the singularity problem in high dimensions and demonstrate that there is little difference between the performance of $T^2$ and that of $T_v^2$. Experiments have demonstrated that the proposed approach is effective in improving the performance of an image retrieval system.

A Study on the Extraction of Evaluation Structure for Conflict Resolution in Coastal Area (연안지역 이해상충 해소를 위한 평가구조 추출에 관한 연구)

  • Yeo, Ki-Tae;Park, Chang-Ho;Yi, Gi-Chul
    • Journal of the Korean association of regional geographers
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    • v.7 no.4
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    • pp.105-119
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    • 2001
  • Currently serious conflicts arose for the use of coastal area in Korea. However, there is no mediation program or mediators' activities for conflict resolution which are shown in the developed countries. Even though, the MOMAF(Ministry of Maritime Affairs and Fisheries) was established in 1997 and the Division of Coastal Zone Management under the Ministry took over the authority to establish ICM program and formulated the CZMA(Coastal Zone Management Act) in 1998 after understanding the seriousness of coastal degradation due to the importance of coastal zone management and the understanding of dispute resolution, it still lacks consistency among legislative power on the continuous policy for wise coastal use and management which results coastal conflicts. The objective of this study is to lay the evaluation criteria for the formalized objective evaluation among disputants of coastal conflicts for the better understanding and characterizing of coastal conflicts in Korea. In order to do so, this study has adopted the PCA(Principal Component Analysis) for the subtraction of the components of evaluation mechanism to describe the present conditions of conflicts in the selected study area(Sihwa lake), to analyze the problems, and then to explore alternative approaches for resolving the conflicts. As research methodologies, we have depended upon literature review and field survey methods. As field survey methods, we employed structured questionnaires for the various samples from the experts of research institutes, professors, representatives of NGOs and citizens. Survey results suggested that 5 representative elements comprising 35 detailed elements could be identified. Based on these results, this study was able to identify and classify the evaluation mechanism and help to resolve coastal conflicts in Korea.

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Brand Image and VMD Strategy of Sports Stores in Korea (2017년 이후 스포츠매장의 브랜드이미지와 VMD 전략)

  • Seo, Jung-Hwa;Kim, Hwa-Kyung;Kim, Jong-Jin;Youn, Myoung-Kil
    • Journal of Distribution Science
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    • v.15 no.11
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    • pp.83-93
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    • 2017
  • Purpose - The study aims to analyze the VMD(Visual Merchandising) perception factors in recent sporting goods store and clarify the effect of each VMD perception factors on brand image, satisfaction, and customer revisit intention. The VMD perception factors play an important role in attracting and actually inducing sales to the visiting customers. It has investigated the effect of VMD perception on customer satisfaction and revisit intention. It is expected that the company's marketing strategy with VMD will be differentiated and competitive in sports item stores, brand image enhancement, customer retention, and acquisition. Research design, data, and methodology - In order to verify the hypotheses of this study, a total of 380 questionnaires had been distributed. 360 respondents were used in the final analysis excluding 20 respondents' incomplete answers. The SPSS 18.0 program was used and the data analysis was conducted for the demographic characteristics and distribution behavior. Principal Components Analysis was used for the common factor extraction for validity analysis, and factor analysis was conducted to verify such as validity in brand image or brand attitude. As for Multiple regression analysis, was performed to verify and in the research model, and in and , the mediation was defined through the Sobel Test in order to verify the brand image mediating effects on VMD, store satisfaction, and revisit inquiry of sports store. Results - Qualitative research shows that VMD sub-variables such as aesthetic, fitness, and functional convenience influence store satisfaction and revisit intention. As a result of analyzing the mediating effect of the brand image, the more VMD is strengthened, the more brand image is improved and store satisfaction is also increased. Conclusions - VMD enhancement requires a VMD strategy aligned with the company's management policies and objectives, a visual directing and consistent concept that delivers a strong message to customers. The customer actual purchasing behavior is a combination of various factors such as sports item stores' interior design, display, advertisement promotion like POP(Point of Purchase), salespersons and their service quality, so that the VMD image and the brand image must be consistent and a unique strategic plan is required.

Effect of Continuous use of Inorganic Fertilizer on the Soil Organisms and Food Chain (무기질비료의 장기연용이 토양생물 및 먹이연쇄에 미치는 영향)

  • Eo, Jinu;Park, Kee-Choon;Park, Jin-Myeon;Kim, Myung-Hyun;Choi, Soon-Kun;Bang, Hea-Seon
    • Korean Journal of Environmental Agriculture
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    • v.35 no.1
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    • pp.39-45
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    • 2016
  • BACKGROUND: This study aimed to evaluate the combined effects of three components (NPK) of chemical fertilizers with basal application of compost on soil organisms.METHODS AND RESULTS: The soil was treated with five treatments continuously for 15 years: control, PK, NK, NP and NPK. The application of N increased plant growth or biomass, and enhanced organic matter content in the soils. Levels of microbial phospholipid fatty acids (PLFAs) in the soils did not show marked differences among the soils treated with different treatments. However, the principal component analysis showed the changes in the structure of the microbial community in the soil, depending on treatments added. Nitrogen application caused a decrease of pH and an increase of EC in the soils, and these environmental stresses appeared to offset the promoting effect of increased organic matter content on microbial abundance. The abundance of bacterivorous nematodes was the highest in the soils after treating NPK; however, the abundance of fungivorous nematodes was unaffected. There was no significant correlation between the abundances of microbial groups and their feeders. Organic matter content was significantly correlated with the abundance of nematodes in the soils.CONCLUSION: Our results showed that chemical fertilizers affect the soil food chains through both biotic and abiotic factors, and a trophic cascade in the soils may not occur in response to long-term fertilization.

Inhibitory Effects of Coptis japonica Alkaloids on the LPS-Induced Activation of BV2 Microglial Cells

  • Jeon, Se-Jin;Kwon, Kyung-Ja;Shin, Sun-Mi;Lee, Sung-Hoon;Rhee, So-Young;Han, Seol-Heui;Lee, Jong-Min;Kim, Han-Young;Cheong, Jae-Hoon;Ryu, Jong-Hoon;Min, Byung-Sun;Ko, Kwang-Ho;Shin, Chan-Young
    • Biomolecules & Therapeutics
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    • v.17 no.1
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    • pp.70-78
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    • 2009
  • Coptis japonica (C. japonica) is a perennial medicinal plant that has anti-inflammatory activity. C. japonica contains numerous biologically active alkaloids including berberine, palmatine, epi-berberine, and coptisine. The most well-known anti-inflammatory principal in C. japonica is berberine. For example, berberine has been implicated in the inhibition of iNOS induction by cytokines in microglial cells. However, the efficacies of other alkaloids components on microglial activation were not investigated yet. In this study, we investigated the effects of three alkaloids (palmatine, epi-berberine and coptisine) from C. japonica on lipopolysaccharide (LPS)-induced microglial activation. BV2 microglial cells were immunostimulated with LPS and then the production of several inflammatory mediators such as nitric oxide (NO), reactive oxygen species (ROS) and matrix metalloproteinase-9 (MMP-9) were examined as well as the phosphorylation status of Erk1/2 mitogen activated protein kinase (MAPK). Palmatine and to a lesser extent epi-berberine and coptisine, significantly reduced the release of NO, which was mediated by the inhibition of LPS-stimulated mRNA and protein induction of inducible nitric oxide synthase (iNOS) from BV2 microglia. In addition to NO, palmatine inhibited MMP-9 enzymatic activity and mRNA induction by LPS. Palmatine also inhibited the increase in the LPS-induced MMP-9 promoter activity determined by MMP-9 promoter luciferase reporter assay. LPS stimulation increased Erk1/2 phosphorylation in BV2 cells and these alkaloids inhibited the LPS-induced phosphorylation of Erk1/2. The anti-inflammatory effect of palmatine in LPS-stimulated microglia may suggest the potential use of the alkaloids in the modulation of neuroinflammatory responses, which might be important in the pathophysiological events of several neurological diseases including Alzheimer's disease (AD), multiple sclerosis (MS), Parkinson's disease (PD) and stroke.