• Title/Summary/Keyword: Priming Effect

Search Result 247, Processing Time 0.03 seconds

The Effects of Mycobacterium Tuberculosis on Alveolar Macrophages -The Alterations of Superoxide Production in both Human and Rat Alveolar Macrophages Exposed to Mycobacterium Tuberculosis H37Ra Strain- (결핵균이 폐포대식세포의 기능에 미치는 영향에 관한 연구 -H37Ra 결핵균종에 의한 사람 몇 백서 폐포대식세포의 Superoxide 생성의 변화-)

  • Kim, Keon-Youl;Lee, Kye-Young;Hyun, In-Kyu;Kim, Young-Whan;Han, Sung-Koo;Shim, Young-Soo;Han, Yong-Chol
    • Tuberculosis and Respiratory Diseases
    • /
    • v.39 no.6
    • /
    • pp.526-535
    • /
    • 1992
  • Background: The oxygen radicals released by alveolar macrophages contribute to killing of microorganisms including M. tuberculosis. Macrophages are "primrd" for enhanced oxygen radical release by macrophage activator like IFN-$\gamma$ and LPS, which do not themselves cause release of oxygen radicals. Actural production of oxygen radicals is "triggered" by phagocytosis or by exposure to chemical stimuli like PMA or FMLP. There has been debates about the priming effect of alveolar macro phages because they are exposed to usual environmental particles unlike blood monocytes. Therefore we examined priming effect of IFN-$\gamma$ in human alveolar macrophages comparing with that in blood monocytes and rat alveolar macrophages. And we observed the alterations of superoxide production in both human and rat alveolar macrophages after exposure to M. tuberculosis H37Ra bacilli itself and its lysate. Methods: Bronchoalveolar lavage fluid was processed to isolate alveolar macrophages by adherence and the adherent cells were removed by cold shock method. After exposure to M. tuberculosis H37Ra strain, alveolar macrophages were incubated for 24 hours with IFN-$\gamma$. The amount of superoxide production stimulated with PMA was measured by ferricytochrome C reduction method. Results: 1) The priming effect in human alveolar macrophages was not observed even with high concentration of IFN-$\gamma$ while it was observed in blood monocytes and rat alveolar macrophages. 2) Both human and rat alveolar macrophages exposed to avirulent H37Ra strain showed triggering of superoxide release and similar results were shown with the exposure to H37Ra lysate. Conclusion: The priming effect in human alveolar macrophages is not observed because of its usual exposure to environmental particles and avirulent H37Ra strain does not inhibit the activation of alveolar macrophages.

  • PDF

What Brings Customer Gapjil? The Intertwined Effects of Perceived Economic Mobility, Self-Other Referent Priming, and Temporal Focus

  • Kwon, Yongju;Yi, Youjae
    • Asia Marketing Journal
    • /
    • v.21 no.4
    • /
    • pp.1-24
    • /
    • 2020
  • The current research brings the spotlight onto customer Gapjil toward service employees. In an attempt to understand what brings Gapjil, the present article investigates the intertwined effects of perceived economic mobility (PEM), self-other referent priming (SORP), and temporal focus on Gapjil. Study 1 shows that PEM increases Gapjil among self-referent primed people, but not among other-referent primed people. Study 2 examining the role of temporal focus (present vs. future) reveals that the effect found in study 1 is replicated in the present focus, but the effect is reversed in the future focus. We explain this dynamic pattern of the 3-way interaction effect with a relative gratification and a motivation to attune the self to the perceived norm of the high class. We also discuss how to create a social atmosphere to lessen Gapjil in public and corporate communication strategies.

Effect of Osmotic Priming and Solid Matrix Priming to Improved Seed Vigor and Early Growth of Pepper and Tomato Seeds (고추와 토마토 종자의 발아력 증진과 초기생육에 미치는 Osmotic Priming 및 Solid Matrix Priming 처리 효과)

  • 강점순;최영환;손병구;이용재;안종길;최인수;박현철
    • Journal of Life Science
    • /
    • v.13 no.4
    • /
    • pp.433-440
    • /
    • 2003
  • Osmotic and solid matrix priming treatments enhanced germination performance. We compared osmotic with solid matrix priming to determine the more effective treatment for improving seed germination in pepper and tomato. Seed hydration was immediately observed after osmotic priming and solid matrix priming treatment. The moisture content of solid matrix primed seeds was lower than that of osmotic primed seeds in the two vegetable crops. Osmotic priming and solid matrix priming did not increased percent germination, but showed shorter number of days to 50% of the final germination percentage ($T_{50}$) compared with untreated seeds, regardless of germination temperature. $T_{50}$ value was reduced in osmotic or solid matrix primed pepper seeds about 6.0, 5.0, 4.6 and 4.0 days compared with untreated seeds at 15, 20, 25, and $30^{\circ}C$, respectively. While, that in tomato seeds was reduced about 3.3, 5.0, 4.6 and 4.0 days compared with untreated seeds at 15, 20, 25, and $30^{\circ}C$, respectively. The effectiveness of osmotic priming or solid matrix priming in reducing the $T_{50}$ was greater when the seeds were germinated at $15^{\circ}C$ than at temperature of higher than $20^{\circ}C$. Solid matrix primed seeds germinated faster than osmotic primed seeds at all temperature in pepper. However, there was no significant difference on the percentage germination between solid matrix and osmotic primed seeds in tomato. After priming, dried-bark seeds showed faster germination than surface-dried seeds in pepper. On the other hand, there was no significant difference in tomato. Emergence of pepper and tomato seeds was markedly enhanced by osmotic priming or SMP treatment although the final emergence percentage was not significantly influenced. On the other hand, early growth was not significantly influenced by osmotic priming or SMP treatment of pepper and tomato seeds.

Optimum Conditions for Tobacco Seed Priming by PEG 6000

  • Tai-Gi, Min;Byung-Moon, Seo
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.44 no.3
    • /
    • pp.263-266
    • /
    • 1999
  • Tobacco (Nicotiana tabacum L. ‘KF109’) seeds were primed in polyethylene glycol 6000 (PEG 6000) solutions to determine a) what osmotic potential of the solution would be optimal for priming, i.e., critical potential level for preventing germination, and b) what temperature and duration would be the most effective in priming. The germination was completely prevented below -0.8 MPa of PEG 6000, that indicates a optimum water potential for seed priming. Seeds were primed for 0, 1, 2, 3, 5, 10 and 15 days at 15, 20 and $25^{\circ}C$, respectively, under the-0.8 MPa PEG 6000 solution to find out the most effective temperature and duration for priming. The effectiveness of priming, particularly in germination speed, was observed more distinctly when the primed seeds were germinated at 15$^{\circ}C$ than 2 5$^{\circ}C$. The greatest reduction of the time to 50% germination (T/sob 50/) was when the seeds were primed at $25^{\circ}C$. The reduction rate of the $T_{50}$ was rapid when primed from 1 day to 8 days and then slowed down in the seeds primed for longer than 8 days. The time from 10 to 90% germination ( $T_{10-90}$ increased in the primed seeds for longer than 8 days which showed the reversed effect of synchronous germination. However, $T_{50}$ was reduced continuously in the seeds even primed over 8 days. Thus, the optimum condition for tobacco seeds priming with PEG 6000 solution was -0.8 MPa in osmotic potential of the solution at $25^{\circ}C$ for 8 days.ays.

  • PDF

Effects of Temperature, Light Condition, and Priming Treatment on Seed Germination of Bidens bipinnata L. (온도, 광 및 Priming 처리가 도깨비바늘 종자의 발아에 미치는 영향)

  • Lee, Sang Yong;Baek, Jun Pill
    • Journal of Bio-Environment Control
    • /
    • v.22 no.2
    • /
    • pp.188-191
    • /
    • 2013
  • This study was carried out to investigate the effects of light, temperature, and priming treatment on the germination of Bidens bipinnata seeds for the purpose of cultivation of new vegetable crop. The results showed that the treatment of $25^{\circ}C$ yielded the highest germination percentages and the highest germination speed in both light and dark conditions. Light condition had no effect on the germination of B. bipinnata. Priming treatments demon-strated the following improved germination percentages: $Ca(NO_3)_2$ 0.1 M (68.3%), $K_3PO_4$ 0.1 M (63.3%), and $KNO_3$ 0.1 M (53.3%), with $Ca(NO_3)_2$ 0.1 M treatment giving the greatest improvement. Consequentially, the results suggested that $25^{\circ}C$ light condition, and $K_3PO_4$ 0.1 M or $Ca(NO_3)_2$ 0.1 M priming treatments would be effective method to improve the germination of Bidens bipinnata seeds.

Effect of Preservation Period, Light, Temperature, and Priming on the Seed Germination of Lysimachia mauritiana (자생 갯까치 수영(Lysimachia mauritiana)의 종자발아에 미치는 저장기간, 광, 온도 및 Priming 처리의 영향)

  • 안영희;설종호;조근호
    • Korean Journal of Environment and Ecology
    • /
    • v.12 no.1
    • /
    • pp.9-13
    • /
    • 1998
  • Because Lysimachia mauritiana is not able to maintain the seed viablity for a long time, it has difficulties in using for the ornamental plants or landscape gardening. This study was conducted to elucidate the optimal germination conditions of Lysimachia mauritiana seeds. The germination rate of the seeds was higer in light condition than in dark one. In experment determining the proper germination temperature, the germination rates were 2.2%, 96.9% and 91.2% under 35$\circ $C, 25$\circ $C, and 20$\circ $C, respectively in light condition. The approprate temperature for the germination was 25$\circ $C in light. The decreases of germination rate with the increase of the preservation periods was able to recover by priming pretreated with NaCl solution and KNO$_{3}$ + K$_{3}$H$_{2}$PO mixed solutions. When the storage period was 300 days, the germination rate was 75% without priming treatments. But the seeds primed with NaCl 0.1M and KNO$_{3}$ + K$_{3}$H$_{2}$PO mixed solutions 0.1M, showed germination rate above 90%. T$_{50}$ also was shorten from 5.25 without priming to 3.78 with NaCl 0.1M. Seed priming may induce earlier sprouts and increase germination rate of long-preserved seeds of native wild plants.

  • PDF

Effect of Plant Growth Regulators and Seed Priming Treatment on the Germination and Early Growth of Snapdragon (Antirrhinum majus L.) (식물생장조절제 및 priming 처리가 금어초 종자의 발아와 초기생육에 미치는 영향)

  • Kang Jum-Soon;Choi In-Soo
    • Journal of Life Science
    • /
    • v.16 no.3 s.76
    • /
    • pp.493-499
    • /
    • 2006
  • In this study, we investigated the effect of growth regulators, seed priming, and light condition for the germination and early growth in Snapdragon (Antirrhinum majus L.). The optimum concentration of growth regulator for the promotion of germinability turned out to be 250 uM of $GA_3$. The germination enhancement in combination of $GA_3+BAP$ was not significant. The optimum priming condition for the maximum germinability was 2 day treatment of 200 mM of $KNO_3$. which increased germination by 14% and shortened the day to germination by 3.5 days. The optimum temperature for germination was $15{\sim}25^{\circ}C$. The germination frequency was decreased to lower than 20% at $30^{\circ}C$, which showed that seeds of Snapdragon germinates better at low temperature than high temperature. The germination frequency was different at light condition; it was low at dark condition, but was increased by $15{\sim}20%$ at red light condition. The combination of $GA_3$ and seed priming showed better germinability than the single treatment of $GA_3$ and seed priming.

Effect of Watching War Documentary on Audience's Security Consciousness - Focusing on 'KBS Special, 100 Days of Invasion of Ukraine, Into the Fire' - (전쟁 다큐멘터리 시청이 수용자의 안보 의식에 미치는영향 - 'KBS 특집, 우크라이나 침공 100일, 포화속으로'를 중심으로-)

  • Park, DugChun
    • Journal of Korea Multimedia Society
    • /
    • v.25 no.11
    • /
    • pp.1613-1620
    • /
    • 2022
  • Through previous studies, it was found that news from legacy media, including television, has an agenda-setting effect and priming effect on the perceptions and attitudes of audiences about politics and war, and that film media also has an agenda-setting effect and political priming effect on war issues. However, it is difficult to find studies on the effects of war-related TV documentaries on media audiences. Therefore, in this study, An experimental study was conducted to investigate whether there is a change in 'recognition of the importance of security', 'will for South-North Unification' and 'will to participate in war in case of emergency' for the audience who watched the KBS special <100 Days of Invasion of Ukraine, Part 1 into the Fire>. As a result of the analysis, it was found that watching a war-related TV documentary reinforced the audience's 'recognition of the importance of security' and 'will for South-North Unification'. However, it was confirmed that watching a war-related TV documentary did not strengthen the audience's will to participate in war in case of emergency.