• Journal of Preventive Medicine and Public Health
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• 제7권1호
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• pp.95-100
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• 1974

1973년(年) 5월(月) 15일(日)부터 동년(同年) 6월(月) 16일(日)까지 약(約) 1개월간(個月間)에 걸쳐 광주시내(光州市內)에 근무(勤務)하고 있는 초(初) 중(中) 고교사(高敎師)와 은행원(銀行員) 및 행정공무원(行政公務員) 등(等) 봉급대상자(俸給對象者) 400명(名)을 조사대상(調査對象)으로 끽연(喫煙) 및 음주(飮酒)에 관(關)한 태도(態度)를 조사(調査)한 결과(結果) 요약(要約)하면 다음과 같다. 1. 끽연자(喫煙者)가 144명(名)(36.%), 비끽연자(非喫煙者)는 256명(名)(64.0%)이며 성별(性別) 끽연율(喫煙率)은 여자(女子)가 1명(名)(0.7%)이고 남자(男子)는143명(名)(54.3%)이었다. 2. 끽연자(喫煙者)의 1일(日) 끽연량(喫煙量)은 권연(卷煙) $11{\sim}20$개비를 피우는 자(者)가 59.0%로 과반수(過半數)이었고 10개비 이하(以下)가 36.8%, $21{\sim}30$개비를 피우는 자(者)가 1.4%로 나타났다. 3. 음주자(飮酒者)가 175명(名)(43.7%), 비음주자(非飮酒者)가 225명(名)(56.3%)이었으며 성별(性別)로는 남성(男性)이 63.8%, 여성(女性)이 2.7%였다. 4. 일회(一回) 음주량(飮酒量)은 5합(合) 이하(以下)가 114명(名)(65.1%)으로 과반수(過半數)이었고 $6{\sim}10$합(合) 정도(程度)가 37명(名)(21.1%), $11{\sim}20$합(合) 정도(程度)가 10.3%, 20합이상(合以上) 2.9%이다.

• 한국균학회지
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• 제32권2호
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• pp.112-118
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• 2004

한국산 흰구름버섯(Trametes hirsuta S1)로부터 배지 내로 분비된 laccase를 ultrafiltration과 anion exchange chromatography, adsorption chromatography를 이용하여 분리 정제하고 정제된 효소의 특성을 조사하였다. Laccase는 균주의 일차 대사 과정에서 주로 생산되는 세포의 페놀 산화효소였다. 흰구름버섯을 기본 배지에서 배양하였을 때 생장은 배양 6일까지 급속히 이루어졌고, laccase의 활성은 배양 5일에 최대활성을 나타냈으며 배양액에서 LiP와 VAO의 활성은 측정되지 않았다. Laccase의 생산에 미치는 유도원의 영향을 조사하기 위하여 배양 중인 흰구름버섯에 몇몇 유도원을 첨가한 결과, 2,5-xylidine은 대조구에 비하여 laccase의 생산을 약4배 증가 시켰다. 정제된 laccase는 SDS 젤 전기영동에서 대략 66 kDa의 분자량을 가지는 단일 폴리펩타이드(single polypeptide)였고, 탄수화물 함량은 12%였다. 정제된 laccase의 $K_m$과 $V_{max}$를 ABTS[2,2-azino-bis(3-ethylbenzthiazo line-6-sulfonic acid)]를 기질로 사용하여 조사한 결과 각각 $51.2\;{\mu}M$과 $56.8\;{\mu}mole{\cdot}min^1{\cdot}mg^{-1}$로 측정되었다. Laccase 활성의 최적 pH는 3.0이며, 이 효소는 $50^{\circ}C$ 미만에서 1시간 동안 처리하였을 때 안정적이었고 $70^{\circ}C$에서 20분간 처리하였을 때 효소의 활성이 반감되었다. Laccase의 분광학적 특성을 조사한 결과 구리를 포함하는 단백질로 나타났다. 일반적으로 알려진 laccase의 기질들에 대한 특이성을 조사한 결과, 5 mM ABTS에서 가장 높은 활성을 나타내었으며 tyrosine에서는 laccase의 활성이 나타나지 않았다. 저해제의 영향을 조사한 결과, 일반적으로 구리를 포함하는 단백질의 저해제인 $NaN_3$, TGA, DDC를 일정 농도로 처리한 실험구에서는 효소의 활성이 완전하게 억제되었으며, EDTA 처리구에서는 효소의 활성이 억제되지 않았다. 한국산 흰구름버섯 S1 균주로부터 생산되는 laccase의 N-말단의 아미노산의 서열은 Coriolus hirsutus의 laccase와 100%의 상동성을 나타냈고, T. versicolor의 laccase I과는 68%의 상동성을 나타냈다.

• 한국수산과학회지
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• 제8권2호
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• pp.107-117
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• 1975

Alaska pollack caught in the Northern Pacific Ocean and frozen aboard vessel are skipped to the plant and processed into frozen fillets. In the present paper quality changes during thwaing, refreezing and storage at $-20^{\circ}C$ are discussed. Natural, running-water, vacuum and steam thawing were employed as thawing methods. And contact plate, air blast, immersion in dry ice-alcohol solution freezing and storage at $-5^{\circ}C$ were applied to refreeze the thawed fillets. As quality factors content of drip released, salt-extractable protein, VBN, DNA in the drip and pH were determined. In addition, bacteriological tests were also carried out along with the whole process. In thawing of round material, the vacuum thawing was more effective than any other method, resulting in drip, salt-extractable protein $(N\%)$, VBN and DNA as $4.4\%,\;1.82\%,\;16.21mg\%$ and $13.70\;{\mu}g/ml$, respectively. Storage at $-5^{\circ}C$ as refreezing method yielded lower in drip and DNA content but similar to or slightly higher in both salt-extractable protein and VBN, which might postulate that the quality of the frozen fillet depends not largely on the secondary freezing but on the conditions of thawing and primary freezing. It seemed that most of the bacterial flora in thawed fillet came from skin and viscera of fish, worker's hands, utensils and other processing facilities, since sanitary indicative bacteria were not detected in the frozen flesh of round Alaska pollack. Bacterial quality of fillet varied with thawing methods, vacuum thawing appeared more sanitative compared with other methods as natural, running-water, and steam thawing. Bacterial colonies isolated from the thawed fillet were composed of $73.8\%$ Gram negative rod shape, $4.9\%$ Gram positive rod shape, $18.0\%$ cocci, and $3.3\%$ yeast. Decreasing rate of coliform group of the fillet during the storage at $-20^{\circ}C$ for 30 days was more than $70\%$ and that of plate count was less than of coliform group.

• Applied Biological Chemistry
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• 제51권3호
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• pp.177-182
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• 2008

피혁 제조 공정 중 발생하는 프레싱 스크랩으로부터 회수된 동물성 유지를 이용하여 lipase-catalyst 전이에스테르화 실험을 실시하였다. 실험 결과, 반응온도 $45^{\circ}C$에서 전환율이 극대점을 나타내었으며, 반응온도 $45^{\circ}C$ 이상에서는 전환율이 점차적으로 감소되었다. 초기 효소의 불활성화를 막기 위해서는 1.5몰씩 4단계에 걸쳐 투입하는 것이 가장 높은 전환율을 나타내었으며, 1.5몰 미만의 methanol로 투입 단계를 4단계 초과하여 사용하였을 경우에는 오히려 전환율이 낮아지는 경향을 나타내었다. 종류가 다른 4가지의 리파제를 사용하여 돈지의 전환율에 미치는 영향을 실험한 결과 C. antarctica를 기원으로 하는 Novozyme 435를 사용한 경우 82.2%의 전환율을 나타내어 가장 효과적이었다. 수분 함유량이 증가할수록 전환율이 저하됨을 확인하였다. 1.2wt.%에서의 전환율은 수분이 없는 상태에 비해 약 40%나 낮게 나타나 프레싱 스크랩으로부터 회수된 유지를 정제 공정을 거쳐 수분을 완벽하게 제거하는 것이 전환율을 높이는데 매우 중요한 인자임을 확인하였다. 고정화된 효소를 반복 사용 실험에서는 2회 사용할 때 약간 전환율이 감소하였으나 계속적으로 반복 사용할 때에는 전환율의 감소율이 둔화되었다. 전이에스테르화 반응을 우지에도 적용한 결과 전환율에서 돈지 84%보다 높은 88%를 나타내었다. 이 반응에 의해 생성된 지방산 에스테르는 SOx를 발생시키지 않고, 매연 발생량이 최소화되는 바이오디젤로서 사용될 수 있을 것으로 사료되었다.

• 한국식품과학회지
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• 제39권3호
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• pp.309-314
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• 2007

멥쌀에 홍국 쌀을 각각 0, 10, 20, 30, 40% 첨가하여 제조한 약주의 이화학적 특성 및 관능적 특성의 변화를 조사하였다. 홍국 쌀 첨가량의 증가에 따라 pH는 감소되었고 총산의 함량은 증가된 것으로 나타났다. 총당의 함량은 홍국 쌀 첨가량의 증가에 따라 증가하였고 알코올 함량은 감소하였다. 색도 중 L값은 홍국 쌀 첨가량의 증가에 따라 감소되었으나 a와 b값은 증가한 것으로 나타났다. 향기성분은 esters, aldehydes, alcohols, acids로 분류되었고 과일향의 주성분인 esters의 함량은 10% 홍국 쌀을 첨가한 시험구에서 제일 많이 생산된 것으로 나타났다. 관능검사 결과 전체적 기호도에서 10% 홍국 쌀을 첨가하여 제조한 약주가 제일 우수한 것으로 나타났다. 이화학적 특성이나 관능적 특성으로 보아 10% 홍국 쌀을 첨가하는 약주를 제조하는 것이 적합하다고 생각된다.

• Journal of Yeungnam Medical Science
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• 제13권2호
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• pp.261-271
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• 1996

저자들은 1992년 1월 1일부터 1995년 6월 30일까지 영남대학병원에서 양에너지 방사선 골밀도 측정기로 요추 골밀도를 측정한 자연적 폐경기 여성 136명을 대상으로 설문과 의무기록지를 검토하여 연령, 신장, 체중, 초경 및 폐경 연령, 자녀수, 모유수유 자녀수, 경구 피임력, 골다공증의 가족력, 우유 및 커피섭취량, 흡연 및 음주력 그리고 신체활동량을 평가하였다. 평균 연령은 55.2세였고, 평균 폐경 연령은 47.9세였다. 골밀도는 신장, 체중 그리고 신체활동량과 유의한 양의 상관관계를 보였고, 나이, 폐경 후 기간 그리고 자녀수와는 유의한 음의 상관관계를 보였다. % age-matched 골밀도는 나이, 신장, 체중, 신체활동량 그리고 폐경 후 기간과 유의한 상관관계를 보였다. 골밀도를 종속변수로 한 다중회귀분석에서는, 폐경 후 기간, 신체활동량 그리고 체중이 골밀도에 영향을 주는 유의한 변수였고, 그 중 폐경 후 기간이 가장 영향을 주는 변수였다. 나이의 효과를 통제하기 위해 % age-matched 골밀도를 종속변수로 한 다중회귀분석에서는, 신체활동량과 체중이 % age-matched 골밀도에 영향을 주는 유의한 변수였고, 신체활동량이 가장 영향을 주는 변수였다. 그 결과 폐경기 여성의 골밀도에 영향을 미치는 인자들 중 나이의 영향을 배제하면 신체활동량과 체중이 중요한 인자로 생각된다. 그러므로 지속적인 신체활동이 골다공증의 예방에 중요하다는 것을 알 수 있었다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권9호
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• pp.3595-3604
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• 2015

Hepatocellular carcinoma (HCC) has been one of the most fatal malignant tumors worldwide and its associated morbidity and mortality remain of significant concern. Based on in-depth reviews of serological diagnosis of HCC, in addition to AFP, there are other biomarkers: Lens culinaris agglutinin-reactive AFP (AFP-L3), descarboxyprothrombin (DCP), tyrosine kinase with Ig and eprdermal growth factor (EGF) homology domains 2 (TIE2)-espressing monocytes (TEMs), glypican-3 (GPC3), Golgi protein 73 (GP73), interleukin-6 (IL-6), and squamous cell carcinoma antigen (SCCA) have been proposed as biomarkers for the early detection of HCC. The diagnosis of HCC is primarily based on noninvasive standard imaging methods, such as ultrasound (US), dynamic multiphasic multidetector-row CT (MDCT) and magnetic resonance imaging (MRI). Some experts advocate gadolinium diethyl-enetriamine pentaacetic acid (Gd-EOB-DTPA) MRI and contrast-enhanced US as the promising imaging madalities of choice. With regard to recent advancements in tissue markers, many cuting-edge technologies using genome-wide DNA microarrays, qRT-PCR, and proteomic and inmunostaining studies have been implemented in an attempt to identify markers for early diagnosis of HCC. Only less than half of HCC patients at initial diagnosis are at an early stage treatable with curative options: local ablation, surgical resection, or liver transplant. Transarterial chemoembolization (TACE) is considered the standard of care with palliation for intermediate stage HCC. Recent innovative procedures using drug-eluting-beads and radioembolization using Yttrium-90 may exhibit beneficial effects in HCC treatment. During the past few years, several molecular targeted agents have been evaluated in clinical trials in advanced HCC. Sorafenib is currently the only approved systemic treatment for HCC. It has been approved for the therapy of asymptomatic HCC patients with well-preserved liver function who are not candidates for potentially curative treatments, such as surgical resection or liver transplantation. In the USA, Europe and particularly Japan, hepatitis C virus (HCV) related HCC accounts for most liver cancer, as compared with Asia-Pacific regions, where hepatitis B virus (HBV) may play a more important role in HCC development. HBV vaccination, while a vaccine is not yet available against HCV, has been recognized as a best primary prevention method for HBV-related HCC, although in patients already infected with HBV or HCV, secondary prevention with antiviral therapy is still a reasonable strategy. In addition to HBV and HCV, attention should be paid to other relevant HCC risk factors, including nonalcoholic fatty liver disease due to obesity and diabetes, heavy alcohol consumption, and prolonged aflatoxin exposure. Interestingly, coffee and vitamin K2 have been proven to provide protective effects against HCC. Regarding tertiary prevention of HCC recurrence after surgical resection, addition of antiviral treatment has proven to be a rational strategy.

• Asian Pacific Journal of Cancer Prevention
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• 제16권17호
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• pp.7619-7625
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• 2015

Background: Aberrant microRNA expression has been associated with the pathogenesis of a variety of human malignancies including oral squamous cell carcinoma (SCC). In this study, we examined primary oral SCCs for the expression of 6 candidate miRNAs, of which five (miR-34a, miR-143, miR-373, miR-380-5p, and miR-504) regulate the tumor suppressor TP53 and one (miR-99a) is involved in AKT/mTOR signaling. Materials and Methods: Tumor tissues (punch biopsies) were collected from 52 oral cancer patients and as a control, 8 independent adjacent normal tissue samples were also obtained. After RNA isolation, we assessed the mature miRNA levels of the 6 selected candidates against RNU44 and RNU48 as endogenous controls, using specific TaqMan miRNA assays. Results: miR-34a, miR-99a, miR-143 and miR-380-5p were significantly down-regulated in tumors compared to controls. Moreover, high levels of miR-34a were associated with alcohol consumption while those of miR-99a and miR-143 were associated with advanced tumor size. No significant difference was observed in the levels of miR-504 between the tumors and controls whereas miR-373 was below the detection level in all but two tumor samples. Conclusions: Low levels of miR-380-5p and miR-504 that directly target the 3'UTR of TP53 suggest that p53 may not be repressed by these two miRNAs in OSCC. On the other hand, low levels of miR-34a or miR-143 may relieve MDM4 and SIRT1 or MDM2 respectively, which will sequester p53 indicating an indirect mode of p53 suppression in oral tumors.

• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.3731-3740
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• 2012

Background: There have been few studies of Asian ovarian cancer and benign tumors. The primary aim of this paper was to report the protocol of the Ko-EVE study to examine epidemiological and molecular factors for ovarian cancer and benign neoplasms and to ascertain the major risk factors for ovarian cancer control in Korea. Methods: This case-control study covers incident epithelial ovarian cancers and benign neoplasms, four major centers participating in enrolling incident cases and 3 hospitals enrolling healthy controls among health examinees. Standardized questionnaires were administered by trained interviewers, including sections on socio-demographics characteristics, past medical history, medication usage, family history, lifetime consumption of alcohol and tobacco, diet, physical activity, and reproductive factors for women. Various biological specimens were collected in the biorepository according to the standardized protocol. Annual follow-up for cancer cases and follow-up at the 1st year for benign tumor cases are performing to evaluate treatment effect and progression. Passive follow to see long-term survival will be conducting using record linkage with national data. Results: The total number recruited in 2010-2011 was 246 epithelial ovarian cancer cases, 362 benign epithelial tumors and 345 controls. We are planning to collect subjects for at least 1,500 sets of ovarian cancer, 2,000 benign tumors and 1,500 controls till 2018. Conclusions: The Ko-EVE will provide unique and important data to probe the etiology and natural history of Korean epithelial ovarian cancer. It will be continued by genomic and proteomic epidemiological analyses and future intervention studies for the prevention of ovarian cancer among Koreans.

• 사상체질의학회지
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• 제15권3호
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• pp.88-99
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• 2003

Background and Objective : The status of development of instruments to assess the 'health status' reflecting the concept of Sasang Constitutional Medicine is still far from satisfaction, despite their importance in building basic data for health promotion, evaluation of effectiveness of treatment, health policy and so on. for these reasons the health scale of Sasang Constitutional Medicine shod be developed. Therefore as from of preliminary research, this study is to review the concept of health and symptoms in health status in Sasang Constitutional Medicine. Methods : It was researched as bibliologically with Dong-mu's chief medical writings such as ${\ulcorner}Dongyi$ Soose $Bowon{\lrcorner}$(東醫壽世保元)${\ulcorner}Dongyi$ Soose Bowon Sasang Chobongyun(東醫壽世保元四象草本卷)${\lrcorner}$ - Results and conclusion : 1. In Sasang Constitutional Medicine, it is suggested that Inherent vitality(命脈實數) has relations to health and Knowledge-Acting(知行) is a primary factor which affects health. And it is thought that Healthy condition(完實無病) is that human being has enough Healthy energy(保命之主) of Small viscera. 2. In Sasang Constitutional Medicine, it is thought that stool, urine, sweating and digestion become important indexes to measure the physical health state. 3. In Sasang Constitutional Medicine, it is thought that the mental health is in control of inclination of mind by golden mean(中庸) and in tranquillity of constant mind according to constitution. 4. In Sasang Constitutional Medicine, it is thought that the social health is state thar can do social acting harmoniously by keeping away from alcohol, sexual appetite, property and power.