• Journal of Veterinary Clinics
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• v.26 no.6
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• pp.595-599
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• 2009

Sarcocystis grueneri-like sarcocysts were found from the cardiac muscles of a rearing red deer (Cervus elaphus) carcass in Korea. In the light microscopical examination of sarcocysts, they were oval to spherical cysts and 90-170$\times$110-380 ${\mu}m$ in size. However, there was no inflammation and myofiber degeneration. In the transmission electron microscope, these cysts were located within the sarcoplasm of the host cell and filled with merozoites. The sarcocysts were enclosed by a very thin wall (0.45-0.6 ${\mu}m$ thick) that consists of protrusions and ground substance. The primary cyst wall formed numerous strip-like protrusions which were 0.2-0.3 ${\mu}m$ wide and up to 4.2 ${\mu}m$ long. The protrusions were running in parallel with the surface of the cyst. A characteristic of the cyst wall was absent of fibrils inside the protrusions. Merozoites in the compartment measured about $15\times4\;{\mu}m$. The merozoite consisted of four regions: micronemes and rhoptries, amylopectin granule, nucleus, and amylopectin granules. The number of rhoptry was counted in 7-13.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.21 no.4
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• pp.332-344
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• 1999

Cytokines are hormone-like proteins which mediate and regulate inflammatory and immune responses. Interleukin-6 (IL-6) is involved in the final differentiation of B cells into antibody-producing cells. Interleukin-8 (IL-8) is a neutrophil chemotactic factor that plays an important role in the recruitment of neutrophil to inflammatory loci. Inflammatory mediators by cells in the gingiva have been implicated in the initiation and progression of periodontitis and oral infection. The purpose of this study was conducted to investigate the effect of lipopolysaccharide (LPS), staphylococcus enterotoxin B (SEB) on production of IL-6 and IL-8 by human gingival and facial dermal fibroblasts. Primary cultured human gingival and facial dermal fibroblasts were incubated with LPS (0.01, 0.1, $1.0{\mu}g/ml$), SEB (0.01, 0.1, $1.0{\mu}g/ml$) or LPS $(0.1{\mu}g/ml)$ plus SEB $(0.1{\mu}g/ml)$. Culture supernatants were collected at 24, 48, and 72 hrs and assessed for IL-6 and IL-8 production by enzyme-linked immunosorbent assay. IL-6 production in gingival fibroblasts stimulated with LPS was higher than that with SEB. IL-6 production by double exposure with LPS plus SEB was amplified in comparison with single exposure of LPS or SEB. IL-6 production in facial dermal fibroblasts was increased only by stimulation with a high concentration of LPS $(1.0{\mu}g/ml)$. Its production in facial dermal fibroblasts by exposure with SEB was decreased in comparison with control, nontreated cells. Therefore, gingival fibroblasts showed higher sensitivity than facial dermal fibroblasts in response to low concentration of LPS. Also, IL-6 production by double exposure with LPS plus SEB was amplified in comparison with single exposure of LPS or SEB. IL-8 production in gingival fibroblasts was enhanced greatly only by stimulation of high concentration of LPS $(1.0{\mu}g/ml)$. That by exposure with SEB was increased only in 24 hrs cultivation. IL-8 production by double exposure with LPS plus SEB was amplified in comparison with single exposure of LPS or SEB. IL-8 production in facial dermal fibroblasts was decreased by LPS and increased only in 48 hrs cultivation by SEB. IL-8 production by double exposure with LPS plus SEB was enhanced only in 48 hrs cultivation in comparison with single exposure of LPS or SEB. therefore, IL-6 and IL-8 production were released at various quantities according to bacterial toxin applied and site of fibroblast harvested. These results suggest that gingival fibroblasts may be concerned with IL-6 and IL-8 related inflammatory response more than facial dermal fibroblasts.

• The Korean Journal of Elementary Counseling
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• v.4 no.1
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• pp.215-236
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• 2005

The Purpose of this study is that self-growth program searches effect self-concept and peer-relationship with elementary school student, and advance following assumption to achieve this purpose and achieved study. First, self-concept point of experiment group students who execute self-growth program will be improved is meaning than self-concept point of control group students. Second, peer-relationship point of experiment group students who execute self-growth program will be improved is meaning than peer-relationship point of control group students. Third, effect that self-growth program gets to self-concept may be meaning difference according to sex. Fourth, difference that impact that self-growth program gets in peer- relationship is meaning according to sex may join. Chose fifth-year student 2 class 68 people (experiment group n=34, control group n=34) in I primary school locating to Jeonrabuk-do Iksan-si to verify above construction for study target. Disposal about experiment group executed over 10th for 60-80 minute 2 times in a week because investigator uses reconstructing self-growth program with virtue research paper such as learning program for own growth of Lee-Hyeong-Deuk (1998). In order to verify the effect after experiment, 1 collected materials for estimation by providing the subject children with questionaires about self-concept and peer-relationship before and after the experiment, and then analyzed the average differences in number of marks between the experiment group and the control group before and after the experiment through and by using One-Way ANOVA, and SPSS 11.0 program. The following is the result what I obtained from the above study. First, there was significant difference is between average difference before and after of experiment group and control group which execute self-growth program in self-concept elevation ($F_{(1,66)} =28.734$, p <.001). From the sub-variable, there was significant difference in academic self ($F_{(1,66)}=6.423$, p<.05), Social Self ($F_{(1,66)}=48.331$, p<.001), Physical Self ($F_{(1,66)}=11.074$, p <.01), sentimental self ($F_{(1,66)}=9.402$, p <.01) Second, there was significant difference is average difference before and after of experiment group and control group which execute self-growth program in peer-relationship promotion ($F_{(1,66)}=24.109$, p <.001). From the sub-variable there W3S Significant difference in trust ($F_{(1,66)}=14.507$, p<.001), respect ($F_{(1,66)}=15.271$, p <.001). Third, there was expose that significant difference does not exist in average self-concept before and after by sex of experiment group which executes self-growth program, and was not shown significant difference in sub-vairable. Fourth, there was expose that significant difference of whole peer- relationship and in respect of sub-variable in average peer-relationship before-after by sex of experiment group which execute self-growth program, but significant difference did not appear in trust. Could get conclusion that self-growth program is effect in elementary school student self-concept elevation and peer-relationship promotion according to these study finding, and confirmed possibility that self-growth program may contribute to change emotional special quality of children positively in education spot.

• Journal of Korea Foundry Society
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• v.20 no.2
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• pp.104-109
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• 2000

Three different white cast irons alloyed with Cr, V, Mo and W were prepared in order to study their abrasion wear behavior in as-cast and heat-treated conditions. The specimens were produced using a 15㎏-capacity high frequency induction furnace. Melts were super-heated to $1600^{\circ}C$, and poured at $1550^{\circ}C$ into Y-block pepset molds. Three combinations of the alloying elements were selected so as to obtain the different types of carbides : 3%C-10%Cr-5%Mo-5%W(alloy No. 1: $M_7C_3$ and $M_6C$), 3%C -10%V-5%Mo-5%W(alloy No. 2: MC and $M_2C$) and 3%C-17%Cr-3%V(alloy No. 3: $M_7C_3$ only). A scratching type abrasion test was carried out in the states of as-cast(AS), homogenizing(AH), air-hardening(AHF) and tempering(AHFT). First of all, the as-cast specimens were homogenized at $950^{\circ}C$ for 5h under the vacuum atmosphere. Then, they were austenitized at $1050^{\circ}C$ for 2h and followed by air-hardening in air. The air-hardened specimens were tempered at $300^{\circ}C$ for 3h. 1 ㎏ load was applied in order to contact the specimen with abrading wheel which was wound by 120 mesh SiC paper. The wear loss of the test piece(dimension: $50{\times}50{\times}5$ mm) was measured after one cycle of wear test and this procedure was repeated up to 8 cycles. In all the specimens, the abrasion wear loss was found to decrease in the order of AH, AS, AHFT and AHF states. Abrasion wear loss was lowest in the alloy No.2 and highest in the alloy No.1 except for the as-cast and homogenized condition in which the alloy No.3 showed the highest abrasion wear loss. The lowest abrasion wear loss of the alloy No.2 could be attributed to the fact that it contained primary and eutectic MC carbides, and eutectic $M_2C$ carbide with extremely high hardness. The matrix of each specimen was fully pearlitic in the as-cast state but it was transformed to martensite, tempered martensite and austenite depending upon the type of heat-treatment. From these results, it becomes clear that MC carbide is a significant phase to improve the abrasion wear resistance.

• The Journal of the Petrological Society of Korea
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• v.25 no.3
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• pp.261-281
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• 2016

Our knowledge of the lithosphere beneath the Korean Peninsula has been improved through petrologic and geochemical studies of upper mantle xenoliths hosted by Quaternary intraplate alkali basalts from Jeju Island. The xenoliths are mostly spinel lherzolites, accompanied by subordinate harzburgite and pyroxenites. The mantle xenoliths represent residual mantle material showing textural and geochemical evidence for at least a three-stage evolution, fractional partial melting, recrystallization, and metasomatism. Their composition primarily controlled by early fractional melt extraction and porphyroclastic and mylonitic fabrics formed in a shear-dominated environment, which was subsequently modified by residual slab-derived fluids (or melts). Modal metasomatic products occur as both anhydrous phase(orthopyroxene) and hydrous phase (phlogopite). Late-stage orthopyroxene is more common than phlogopite. However, chemical equilibrium is evident between the primary and secondary orthopyroxene, implying that the duration of post-metasomatic high temperatures enabled complete resetting/reequilibration of the mineral compositions. The metasomatic enrichment pre-dates the host Jeju Quaternary magmatism, and a genetic relationship with the host magmas is considered unlikely. Following enrichment in the peridotite protolith in the mantle wedge, the upper mantle beneath proto-Jeju Island was transformed from a subarc environment to an intraplate environment. The Jeju peridotites, representing old subarc fragments, were subsequently transported to the surface, incorporated into ascending Quaternary intraplate alkali basalt. The result of this study implies that long term material transfer in the transformation of geotectonic setting from a subarc to intraplate may have played a significant role in the evolution of lithospheric mantle, resulting in the enriched mantle domains, such as EM I or EM II in the lithospheric mantle beneath East Asia.

• Journal of Internet of Things and Convergence
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• v.7 no.3
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• pp.9-14
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• 2021

This paper discovers the energy loss factors through the insulation diagnosis of houses or buildings, and proposes directions for energy efficiency improvement. The energy efficiency factor of a building consists of insulation diagnosis, thermal bridge diagnosis, window diagnosis, airtight diagnosis, and equipment diagnosis. Among the residents and facilities in the energy welfare blind spot, an energy efficiency diagnosis was conducted for one senior citizen building located in Naju-si, Jeollanam-do, and energy efficiency diagnosis was conducted after insulation was installed. Energy measurement, diagnosis and analysis were performed using the IoT-based integrated wired/wireless energy diagnosis platform, Energy Finder. As a result of comparison, an overall energy saving rate of 16.38% was achieved. Annual heating energy consumption per unit area decreased from 333.51kWh before construction to 277.35kWh after construction, and annual cooling energy consumption per unit area decreased from 5.51kWh before construction to 5.22kWh after construction. The annual primary energy consumption per unit area decreased from 464.52kWh before construction to 403.69kWh after construction, and the annual energy cost was reduced from 3,063,307.14 won before construction to 2,641,072.49 won after construction. The additional improvement work is needed on the standards affecting energy efficiency other than insulation.

• Korean Journal of Ichthyology
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• v.33 no.1
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• pp.37-42
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• 2021

This study sought to reveal spawning period and sex inversion of black seabream, Acanthopagrus schlegelii collected off the coast of Tongyeong, Korea. The monthly gonodosomatic index (GSI) showed the same pattern of between both sexes, and it was estimated that black seabream spawned once a year (between April and May). Histological examination revealed the transition from male to female sex, size of fork length bisexual gonad with ovarian (primary hermaphrodite; progress from male to female, MF) was seen at 20.5 to 39.6 cm and bisexual gonad with testicular (post hermaphrodite; female development and Male degeneration, FM) was seen at 26.4 to 50.2 cm. The egg diameters was in the range of 0.02 to 0.60 mm, with the smallest average egg diameter of 0.09 mm in February, the highest average egg diameters of 0.43 mm in August, and the range of fecundity was number of eggs 277,148 (33.9 cm) to 2,772,421 (34.1 cm).

• Journal of Korean Medicine Rehabilitation
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• v.29 no.3
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• pp.35-49
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• 2019

Objectives The purpose of this research is to evaluate the effectiveness of moxibustion theraphy for frozen shoulder through systematic review and meta-analysis. Methods The effect of moxibustion for frozen shoulder was constructed as a key question to search randomized controlled trials (RCTs) in 16 databases without type of publication restriction. Results A total of 790 studies were searched and 22 RCTs were finally selected through primary and secondary selection/exclusion process. In most of the evaluation indexes used in each study, moxibustion treatment for shoulder pain was statistically significant compared with other treatments. A meta-analysis of effective rate in 10 studies comparing moxibustion with acupuncture and acupuncture showed a statistically significant effect of moxibustion treatment. And there was a significant effect on the improvement of the shoulder mobility, but the effect size of shoulder pain was no significant effect for shoulder pain reduction. In addition in the result of meta-analysis of 4 studies' effective rate comparing moxibustion with electronic acupuncture, the effect of moxibustion was significantly higher than that of electronic acupuncture in frozen shoulder. Conclusions Moxibustion therapy was effective in symptoms in patients with frozen shoulder. However because of the high risk of bias in the quality of the included studies, the applicability of this study seems to be limited. It is thought that future randomized controlled trials will need to be designed and performed by reviewing ways to minimize bias.

• Korean Journal of Otorhinolaryngology-Head and Neck Surgery
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• v.61 no.12
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• pp.674-680
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• 2018

Background and Objectives The representative mucin genes in the human airway are MUC5AC and MUC5B, which are regulated by several inflammatory and anti-inflammatory substances. Triptolide (TPL), udenafil, betulinic acid, changkil saponin, and glucosteroid are some of the many anti-inflammatory substances that exist. TPL is a diterpenoid compound from the thunder god vine, which is used in traditional Chinese medicine for treatment of immune inflammatory diseases, such as rheumatoid arthritis, systemic lupus erythematosus, nephritis and asthma. However, the effects of TPL on mucin expression of human airway epithelial cells have yet to be reported. Hence, this study investigated the effect of TPL on lipopolysaccharide (LPS)-induced MUC5AC and MUC5B expression in human airway epithelial cells. Subjects and Method The NCI-H292 cells and the primary cultures of human nasal epithelial cells were used to investigate the effects of TPL on LPS-induced MUC5AC and MUC5B expression using real-time polymerase chain reaction, enzyme immunoassay, and Western blot. Results TPL significantly decreased the LPS-induced MUC5AC and MUC5B mRNA expression and protein production. TPL also significantly decreased the nuclear factor-kappa B (NF-kB) phosphorylation. Conclusion These results suggest that TPL down regulates MUC5AC and MUC5B expression via inhibition of NF-kB activation in human airway epithelial cells. This study may provide important information about the biological role of triptolide on mucus-secretion in airway inflammatory diseases and the development of novel therapeutic agents for controlling such diseases.

• Animal Bioscience
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• v.34 no.2
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• pp.172-184
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• 2021

Objective: Vanin1 (VNN1) is a pantetheinase that can catalyze the hydrolysis of pantetheine to produce pantothenic acid and cysteamine. Our previous studies showed that VNN1 is specifically expressed in chicken liver. In this study, we aimed to investigate the roles of peroxisome proliferators activated receptor α (PPARα) and miRNA-181a-5p in regulating VNN1 gene expression in chicken liver. Methods: 5'-RACE was performed to identify the transcription start site of chicken VNN1. JASPAR and TFSEARCH were used to analyze the potential transcription factor binding sites in the promoter region of chicken VNN1 and miRanda was used to search miRNA binding sites in 3' untranslated region (3'UTR) of chicken VNN1. We used a knock-down strategy to manipulate PPARα (or miRNA-181a-5p) expression levels in vitro to further investigate its effect on VNN1 gene transcription. Luciferase reporter assays were used to explore the specific regions of VNN1 targeted by PPARα and miRNA-181a-5p. Results: Sequence analysis of the VNN1 promoter region revealed several transcription factor-binding sites, including hepatocyte nuclear factor 1α (HNF1α), PPARα, and CCAAT/enhancer binding protein α. GW7647 (a specific agonist of PPARα) increased the expression level of VNN1 mRNA in chicken primary hepatocytes, whereas knockdown of PPARα with siRNA increased VNN1 mRNA expression. Moreover, the predicted PPARα-binding site was confirmed to be necessary for PPARα regulation of VNN1 gene expression. In addition, the VNN1 3'UTR contains a sequence that is completely complementary to nucleotides 1 to 7 of miRNA-181a-5p. Overexpression of miR-181a-5p significantly decreased the expression level of VNN1 mRNA. Conclusion: This study demonstrates that PPARα is an important transcriptional activator of VNN1 gene expression and that miRNA-181a-5p acts as a negative regulator of VNN1 expression in chicken hepatocytes.