• 생명과학회지
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• 제33권5호
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• pp.397-405
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• 2023

항산화제로서 산화적 손상의 방지에 중요한 역할을 하는 것으로 알려진 glutathione (GSH)의 면역 조절에 대한 연구는 현재까지 제대로 이루어지지 않았다. 본 연구에서 우리는 환원형 GSH인 luthione^®이 RAW 264.7 세포에서 면역 강화 효과가 있는지를 조사하였다. 유세포 분석 및 면역 형광 실험의 결과에 의하면, luthione은 대조군 세포에 비해 대식세포의 대표적인 기능인 식세포 활성을 luthione 처리 농도 의적으로 증가시키는 것으로 나타났다. 또한, cytokine array의 결과에 의하면, IL-5, IL-1β와 IL-27의 발현이 luthione이 처리된 세포에서 유의하게 증가하였다. 아울러 luthione에 의한 TNF-α 및 IL-1β의 생성 증가는 그들의 단백질 발현 증가를 통해 이루어졌으며, NO 및 PGE₂와 같은 면역 매개체 유리의 증가는 iNOS 및 COX-2의 발현 증가와 관련이 있었으며, 이는 M1 대식세포 분화 마커인 CD86 발현의 증가와 연관성이 있었다. 그리고 heatmap 분석을 통하여 SOCS1/3 매개 STAT/JAK 신호 전달 경로가 luthione에 의한 면역 조절 증가에 관여함을 확인하였다. 결론적으로, 우리의 결과는 luthione이 M1 macrophage polarization의 분자 조절자로 작용하여 면역 능력을 향상시킬 수 있음을 시사한다.

• 한국재난정보학회 논문집
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• 제19권3호
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• pp.673-688
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• 2023

연구목적: 공사 중에 발생하는 화재는 빈도는 적으나 그 피해가 크다. 최근 물류유통산업의 성장으로 신축하는 물류창고의 공사장이 증가하고 있어 연구대상으로 선정하고 화재예방을 정량적으로 접근하기 위해 화재위험도 평가 Tool의 개발을 통해 공사현장의 재난을 줄이고자 연구를 진행하였다. 연구방법: 공정에서 진행되는 작업을 분류하여 연소의 3요소에서 제어가 어려운 공기(산소)를 제외한 가연물과 점화원을 등급별로 구분하고, 피난안전성을 추가로 대입하여 종합적인 화재위험평가 Tool을 객관적으로 정량화하여 정립하였다. 연구결과: 개발하여 제시한 화재위험도 평가 Tool을 이용하여 화재위험도가 낮은 굴착공사와, 화재위험도가 중간정도인 설비공사, 화재위험도가 높은 마감공사를 샘플링하여 결과값(CGI)을 도출하였다. 결론: 본 연구에서는 건설현장에서 화재발생 위험도를 평가할 수 있는 Tool의 개발로 위험도 평가에 따른 물적조건(가연물)과 에너지조건(점화원)을 제어하여 구체적인 예방대책의 수립과 피난안전성의 평가를 가능하게 하였다. 향후 건설현장의 화재위험도 평가Tool의 적용을 통해 위험을 줄일 수 있는 공정의 계획 수립과 소방설비의 적응성을 평가하는 기준으로 제공되길 기대한다.

• 보건행정학회지
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• 제33권3호
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• pp.243-252
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• 2023

This paper aims to introduce Korea's total current health expenditure (CHE) and National Health Accounts of the year 2021 and their 2022 preliminary figures constructed on the basis of the System of Health Accounts 2011. As CHE includes expenditures for prevention, tracking, and treatment of coronavirus disease 2019 (COVID-19) and compensation for losses to medical institutions from 2020, the details are also introduced. Korea's total CHE in 2021 is 193.3 trillion won, which is 9.3% of gross domestic product (GDP). The preliminary figure in 2022, 209.0 trillion won, exceeded the 200 trillion won line for the first time, and its "ratio to GDP" of 9.7% is expected to exceed the average of Organisation for Economic Co-Operation and Development member countries for the first time. Korea's health expenditures, which were well controlled until the end of the 20th century, have increased at an alarming rate since the beginning of the 21st century, threatening the sustainability of national health insurance. The increase in health expenditure after 2020 is partly due to a temporary increase in response to COVID-19. However, when considering the structure of Korea's health insurance price hike, where the ratchet effect of increased medical expenses works particularly strongly, it is unlikely that the accelerating growth trend that has lasted for more than 20 years will stop easily. More aggressive policies to control medical expenses are required in the national health insurance which not only constitutes the main financing sources of the Korean health system but also has the most powerful policy means in effect for changes in the health care provision.

• 대한본초학회지
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• 제24권4호
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• pp.205-213
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• 2009

Objectives : Cancer incidence is increasing in all countries and chemotherapy-induced peripheral neuropathy (CIPN) in patients undergoing chemotherapeutic agents have been a clinically serious problems. So far therapeutic options for CIPN patients are limited and no confirmed methods have yet been established for dealing with peripheral neuropathy. Therefore this review is to provide an evidence-based summary of oriental medicine and CAM (complementary and alternative medicine) neuroprotective and treatment therapies which have gone through clinical trials. Methods : An overview of the domestic and international papers of adult clinical trials relating management of only CIPN symptoms through 1990 to present were searched by electronic databases. Search key words were chemotherapy-induced neurotoxicity, chemotherapy-induced peripheral neuropathy, chemotherapy toxicity & herb, chemotherapy toxicity & acupuncture, chemotherapy toxicity & CAM. Only English and Korean written papers were reviewed. Total 25 papers were reviewed in this study, 18 papers were retrieved by electronic search. Results : Clinical studies of managing CIPN were rare, two acupuncture clinical studies and four herb medicinal studies were found. Rest of 19 papers were about other CAM clinical studies. Total 25 papers were analyzed, and all interventions were focused on their pain control efficacy. Other 24 trials of potential therapies except one proved to be effective for CIPN, however some described to be inadequate positive or sufficient negative. Conclusions : As most of the studies were pilot studies, interventions for the prevention and treatment of CIPN have to go through prospective confirmatory studies, such as larger scale randomized, double-blinded, placebo controlled clinical trials must be done for the safe and effective use of proposed therapies. Also standard measurement scales have to be developed for the better clinical study of CIPN.

• Journal of Veterinary Science
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• 제24권3호
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• pp.44.1-44.17
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• 2023

Background: Antibiotic resistance is a significant public health concern around the globe. Antimicrobial peptides exhibit broad-spectrum and efficient antibacterial activity with an added advantage of low drug resistance. The higher water content and 3D network structure of the hydrogels are beneficial for maintaining antimicrobial peptide activity and help to prevent degradation. The antimicrobial peptide released from hydrogels also hasten the local wound healing by promoting epithelial tissue regeneration and granulation tissue formation. Objective: This study aimed at developing sodium alginate based hydrogel loaded with a novel antimicrobial peptide Chol-37(F34-R) and to investigate the characteristics in vitro and in vivo as an alternative antibacterial wound dressing to treat infectious wounds. Methods: Hydrogels were developed and optimized by varying the concentrations of crosslinkers and subjected to various characterization tests like cross-sectional morphology, swelling index, percent water contents, water retention ratio, drug release and antibacterial activity in vitro, and Pseudomonas aeruginosa infected wound mice model in vivo. Results: The results indicated that the hydrogel C proved superior in terms of cross-sectional morphology having uniformly sized interconnected pores, a good swelling index, with the capacity to retain a higher quantity of water. Furthermore, the optimized hydrogel has been found to exert a significant antimicrobial activity against bacteria and was also found to prevent bacterial infiltration into the wound site due to forming an impermeable barrier between the wound bed and external environment. The optimized hydrogel was found to significantly hasten skin regeneration in animal models when compared to other treatments in addition to strong inhibitory effect on the release of pro-inflammatory cytokines (interleukin-1β and tumor necrosis factor-α). Conclusions: Our results suggest that sodium alginate -based hydrogels loaded with Chol-37(F34-R) hold the potential to be used as an alternative to conventional antibiotics in treating infectious skin wounds.

• 한국지반공학회논문집
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• 제23권5호
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• pp.77-88
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• 2007

6개의 도심지 굴착현장에서 계측된 현장계측자료를 토대로 다층지반에 설치된 앵커지지 지중연속벽에 작용하는 측방토압과 벽체의 변형을 분석하였다. 앵커지지 지중연속벽에 작용하는 측방토압의 분포는 사다리꼴 모양이며, 최대 측방토압의 크기는 $0.45{\gamma}H$임을 알 수 있다. 그리고 굴착면 상부에서도 $0.1{\gamma}H$의 토압이 작용하는 것으로 나타났다. 제안된 측방토압의 크기는 Terzaghi and Peck(1967), Tschebotarioff(1973) 및 홍원표와 윤중만(1995a)이 제안한 연성벽체의 경험토압보다 약 2배 정도 크다. 지중연속벽의 변형거동은 지지방식과 밀접한 관계가 있는 것으로 나타났다. 앵커지지 지중연속벽의 수평변위는 굴착깊이의 0.1%이내에서, 버팀보 지지방식의 경우에는 굴착깊이의 0.25% 이내에서 발생하고 있어, 벽체의 지지효과는 앵커지지방식이 버팀보 지지방식보다 양호함을 알수 있다. 그리고 지중연속벽의 수평변위는 엄지말뚝으로 시공된 앵커지지 흙막이벽의 시공관리기준인 $\delta=0.25%H$ 보다 작으므로 지중연속벽으로 시공된 굴착현장의 안정성은 상당히 양호함을 알 수 있다.

• 산업융합연구
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• 제21권3호
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• pp.139-148
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• 2023

본 연구는 강원도 일개 대학 4년제 치위생학과 276명을 대상으로 2021년 11월 24일부터 11월 26일까지 구글 온라인설문을 통하여 연구하였다. 본 연구의 목적은 유니폼의 감염인식과 세탁 규정 인식, 감염병 예방 의도를 개인주의 및 집단주의 자아존중감을 통해 알아보는 것으로, 향후 감염병 시기에 학생의 감염 교육 방법 모색을 위해 시행되었다. 통계는 SPSS Statistics 24.0, AMOS 21.0을 사용하여 빈도 분석, 탐색적 요인분석, 확인적 요인분석, 신뢰도 분석, 구조방정식, ANOVA 분석을 시행하였다. 연구결과 유니폼 감염인식, 유니폼 세탁 규정 인식, 자아존중감, 개인주의, 집단주의 감염병 예방 의도의 모형이 적합함을 확인하였고(p<.001), 구조방정식 결과 자아존중감은 개인주의, 집단주의 감염병 예방 의도에 영향을 미치는 것으로 나타났다(p<.001). 집단주의는 유니폼 감염인식, 유니폼 세탁 규정 인식, 감염병 예방 의도에 영향을 미치는 것으로 나타나 감염예방을 위한 인식 변화에 자아존중감과 집단주의가 영향을 미치는 것을 확인하였다. 향후 치위생학과의 감염관리 교육시 집단주의를 고려한 유니폼 세탁 방법에서 활용이 가능할 것이다.

• Journal of Veterinary Science
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• 제23권1호
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• pp.8.1-8.15
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• 2022

Background: Brucella infection induces brucellosis, a zoonotic disease. The intracellular circulation process and virulence of Brucella mainly depend on its type IV secretion system (T4SS) expressing secretory effectors. Secreted protein BspJ is a nucleomodulin of Brucella that invades the host cell nucleus. BspJ mediates host energy synthesis and apoptosis through interaction with proteins. However, the mechanism of BspJ as it affects the intracellular survival of Brucella remains to be clarified. Objectives: To verify the functions of nucleomodulin BspJ in Brucella's intracellular infection cycles. Methods: Constructed Brucella abortus BspJ gene deletion strain (B. abortus ∆BspJ) and complement strain (B. abortus pBspJ) and studied their roles in the proliferation of Brucella both in vivo and in vitro. Results: BspJ gene deletion reduced the survival and intracellular proliferation of Brucella at the replicating Brucella-containing vacuoles (rBCV) stage. Compared with the parent strain, the colonization ability of the bacteria in mice was significantly reduced, causing less inflammatory infiltration and pathological damage. We also found that the knockout of BspJ altered the secretion of cytokines (interleukin [IL]-6, IL-1β, IL-10, tumor necrosis factor-α, interferon-γ) in host cells and in mice to affect the intracellular survival of Brucella. Conclusions: BspJ is extremely important for the circulatory proliferation of Brucella in the host, and it may be involved in a previously unknown mechanism of Brucella's intracellular survival.

• Safety and Health at Work
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• 제14권2호
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• pp.141-152
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• 2023

Objective: We performed a meta-analysis of epidemiological results for the association between occupational exposure as a firefighter and cancer as part of the broader evidence synthesis work of the IARC Monographs program. Methods: A systematic literature search was conducted to identify cohort studies of firefighters followed for cancer incidence and mortality. Studies were evaluated for the influence of key biases on results. Random-effects meta-analysis models were used to estimate the association between ever-employment and duration of employment as a firefighter and risk of 12 selected cancers. The impact of bias was explored in sensitivity analyses. Results: Among the 16 included cancer incidence studies, the estimated meta-rate ratio, 95% confidence interval (CI), and heterogeneity statistic (I²) for ever-employment as a career firefighter compared mostly to general populations were 1.58 (1.14-2.20, 8%) for mesothelioma, 1.16 (1.08-1.26, 0%) for bladder cancer, 1.21 (1.12-1.32, 81%) for prostate cancer, 1.37 (1.03-1.82, 56%) for testicular cancer, 1.19 (1.07-1.32, 37%) for colon cancer, 1.36 (1.15-1.62, 83%) for melanoma, 1.12 (1.01-1.25, 0%) for non-Hodgkin lymphoma, 1.28 (1.02-1.61, 40%) for thyroid cancer, and 1.09 (0.92-1.29, 55%) for kidney cancer. Ever-employment as a firefighter was not positively associated with lung, nervous system, or stomach cancer. Results for mesothelioma and bladder cancer exhibited low heterogeneity and were largely robust across sensitivity analyses. Conclusions: There is epidemiological evidence to support a causal relationship between occupational exposure as a firefighter and certain cancers. Challenges persist in the body of evidence related to the quality of exposure assessment, confounding, and medical surveillance bias.

• 한국환경보건학회지
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• 제49권3호
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• pp.169-177
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• 2023

Background: The Korea Centers for Disease Control and Prevention (KCDC) has identified cases of people suspected of suffering lung disease potentially caused by chloromethylisothiazolinone/methylisothiazolinone (CMIT/MIT) used in humidifier disinfectants (HDs). The Korean Ministry of Environment (MoE) epidemiological investigation and toxicity test study found that HDs caused health damage such as asthma and lung disease. Objectives: The main purposes of this study were to classify the HD exposure rating and to analyze the exposure characteristics that affect exposure to CMIT/MIT HDs. Methods: The exposure characteristics and socio-demographic characteristics of victim participants using CMIT/MIT HDs were investigated through questionnaires. An inhalation no observed adverse effect level (NOAEL) for CMIT/MIT was produced based on inhalation toxicity values. Exposure ratings (class 1~class 2) were cross-tabulated with clinical ratings (acceptable~unacceptable). A correlation analysis was conducted with the main exposure characteristics that affect the exposure concentration of CMIT/MIT HDs. Results: The concentration in indoor air of CMIT/MIT was 8.75±25.40 ㎍/m³, and the exposure concentration was 2.30±6.29 ㎍/m³. The CMIT/MIT exposure rating of 67 participants with high exposures of not more than MOE 100 were evaluated as 14.5%, while the damage participants who matched the clinical rating made up 4.5%. The exposure concentration of CMIT/MIT showed a positive correlation with the daily usage amount and usage frequency, and a negative correlation with volume of the indoor environment. Conclusions: A new exposure rating could be suggested and calculated based on the MOE, and the factors affecting the exposure concentration could be identified.