• 제목/요약/키워드: Pressure Medium

검색결과 795건 처리시간 0.027초

3T3-L1 세포주해서 분비하는 인체 암세포 성장억제 단백질에 대한 연구 (A Study on Growth-inhibiting Protein of Human Cancer Cells Secreted from 373-L1 Cell-line)

  • 은재순;권진
    • Biomolecules & Therapeutics
    • /
    • 제4권1호
    • /
    • pp.46-50
    • /
    • 1996
  • Inhibition of the growth of human cancer cells by proteins secreted from 373-L1 cells was investigated in the present study. The growth of human cancer cells was inhibited by co-culture with 373-L1 cells under 10% FBS and DME, DME, GIT and serumless medium, respectively. The conditioned medium of cultured 373-L1 cells under serumless medium was concentrated 100-fold through an ultrafiltration cell with a 10,000 molecular weight cutoff at 4$^{\circ}C$ under positive pressure using nitrogen(373-L1 EM). 373-L1 EM inhibited the growth of HeLa, Hep G 2, KHOS-Np, A43l and MCF-7 cells. 3T3-L1 EM was purified with FPLC, DEAE-ion exchange chromatography and phenyl-sepharose chromatography. The major protein of 373-L1 EM has a molecular weight of 66,000-68,000 in SDS-PAGE analysis. The results suggest that the inhibitory activity of 373-L1 EM appears to be due to some protein(m.w.66,000-68,000) secreted by 373-L1 cells.

  • PDF

The Optimal Culture Conditions for the Mycelial Growth of Oudemansiella radicata

  • Kim, Sang-Beom;Kim, Seong-Hwan;Lee, Kyung-Rim;Shim, Jae-Ouk;Lee, Min-Woong;Shim, Mi-Ja;Lee, U-Youn;Lee, Tae-Soo
    • Mycobiology
    • /
    • 제33권4호
    • /
    • pp.230-234
    • /
    • 2005
  • Oudemansiella radicata, one of edible mushrooms belonging to Tricholomataceae of Basidiomycota, has been known to exhibit outstanding therapeutic effects on the hypertension caused by high blood pressure and inhibitory effects on the sarcoma 180 and Erhrlich carcinoma of mice. As one of preliminary experiments for producing fruiting-body of O. radicata, this study was carried out to obtain the basic information for culture conditions of mycelial growth of the fungus. The optimal temperature and pH for the mycelial growth were $25^{\circ}C$ and pH 6, respectively. The medium for favorable mycelial growth of O. radicata was shown in the Lilly medium, whereas compact mycelial density was found in Hamada medium. The carbon and nitrogen sources promoting for mycelial growth of O. radicata were xylose and alanine, respectively. The optimum C/N ratio was about 20 : 1 in case that 3% glucose was supplimented to the basal medium as a carbon source.

초음파간섭 수은주압력계와 정적법 중진공 국가표준기 상호비교 (Inter-comparison between ultrasonic interferometer manometer and medium vacuum standards by static expansion method)

  • 홍승수;임인태;신용현;정광화
    • 한국진공학회지
    • /
    • 제14권3호
    • /
    • pp.103-109
    • /
    • 2005
  • 정적법을 이용하여 새로운 중진공 국가표준기를 개발하였다. 이 장치의 성능평가를 위해 측정범위가 각각 133pa과 1,333pa인 두 개의 용량혈 진공게이지를 전달표준기로 사용하여 초음파간섭 수은주압력계와 중진공 표준을 상호비교하였다. Error normalized (En) 값으로 분석한 결과 교정압력 $3pa\;\sim\;100pa$에서 두 표준기는 합성불확도 범위 내에서 잘 일치하고 있음을 알 수 있었다.

통합수자원평가지수의 개발 (Development of Integrated Water Resources Evaluation Index)

  • 이동률;최시중;문장원
    • 한국수자원학회논문집
    • /
    • 제46권10호
    • /
    • pp.1017-1028
    • /
    • 2013
  • 본 연구의 목적은 물이용, 홍수 및 하천환경의 3개 수자원 정책 부문에서 수행한 사업성과를 지역별로 평가할 수 있는 통합수자원평가지수(Integrated Water Resources Evaluation Index, IWREI)를 개발하는 것이다. IWREI는 각 부문별 취약성을 나타내는 물이용취약지수(Water Use Vulnerability Index, WUVI), 홍수취약성지수(Flood Vulnerability Index, FVI), 하천환경취약지수(River Environment Vulnerability Index, REVI)를 통합하여 산정된다. 이들 지수들은 물이용, 홍수, 하천환경의 피해를 주는 압력지표(pressure indicators)와 이에 의한 피해 현상지표(state indicators) 및 피해의 대책지표(response indicators)에서 선정한 총 26개 지표로 구성된다, 산정된 지수는 Low, Medium Low, Medium, Medium High, 및 High의 5단계로 취약성 및 정책수행의 효과를 나타냈다. 우리나라 115개의 중권역에 대해 WUVI, FVI, REVI 및IWREI을 분석한 결과, 1990년대 초반에 비해 2000년대 초반의 지수가 개선되어 수자원 정책의 사업효과를 확인할 수 있다. 본 연구에서 개발된 지역간수자원의 취약지수와 평가지수는 수자원 정책목표의 설정과 사업이행의 우선지역을 선정하는데 이용할 수 있다.

공기구동 기체이젝터의 성능특성에 관한 연구 (A Study on the Performance Characteristics of Air Driven Gas Ejector)

  • 홍영표;윤두호;김용모;윤석훈
    • Journal of Advanced Marine Engineering and Technology
    • /
    • 제18권1호
    • /
    • pp.51-59
    • /
    • 1994
  • The gas jet pumps serve to preduce a vacuum or can be used as gas jet compressors. These are operated on the same principle as a steam jet vacuum pump : in the driving nozzle the pressure energy of the motive medium is converted into the kinetic energy. In the diffuser the driving jet mixes with the suction medium and the kinetic energy is reconverted into the pressure enegy. The application fields of gas jet ejectors are the evacuation of siphoning installations, the elevation of liquids, the production of vacuum filters, the vacuum supporting airlift system, the evacuation of the suction line of centrifugal pumps and the ventilation of the dangerous gases to the atmosphere. The performance of gas jet ejector is influenced strongly to velocity coefficient of motive nozzle, the distance between the motive outlet to the diffuser inlet and the dimensions of diffuser. This study is performed for the computer aided design of gas jet ejectors in future. Through the present experiments, it is known that the velocity coefficient of the motive air nozzle ranges from 0.91 to 0.95 and the maximum efficiency of gas jet ejector is 24.6%.

  • PDF

액체질소중에 있어서 아크방전 및 방사 전자파의 특성에 관한 연구 (A study on the characteristics of arc discharge and radiated electromagnetic wave in liquid nitrogen)

  • 신동화;박광서;주재현;최병주;김기채;이광식;이동인
    • 대한전기학회:학술대회논문집
    • /
    • 대한전기학회 1997년도 하계학술대회 논문집 E
    • /
    • pp.1669-1671
    • /
    • 1997
  • Liquid nitrogen has many adventages. It is cheaper than other cooling medium. It is easy to obtain high purity. It's utilized with easy. In future, liquid nitrogen is able to be accepted cooling medium for the superconducting and cryogenic applicated equipments. Cryogenic liquid may be atmospheric pressure state because thermoinsulation is ineffective, airtight decreasing by long time using and bubble occured by flowing current. If it is to be atmospheric pressure state, the electrical insulation strength is dropped and processed partial discharge, finally breakdown. Processing of breakdown has anticipated by measuring radiated-electromagnetic wave which is happened in time of breakdown. There is a measurement of radiated electromagnetic wave as a method that proceeding breakdown is anticipated. In this paper, radiated electromagnetic wave was measured in arc discharge by above conditions as a basic experiment.

  • PDF

침-평판 전극 사이에서 중간 압력 질소 방전의 시뮬레이션 (Numerical Simulation of Nitrogen Discharge at Medium Pressure between Point-Plane Electrodes)

  • 고욱희;박인호
    • 한국진공학회지
    • /
    • 제17권6호
    • /
    • pp.487-494
    • /
    • 2008
  • 준 2차원 수치적 모델을 사용하여 침-평판(point-plane)형의 전극 사이에 있는 중간 압력 질소의 방전에 대한 시뮬레이션을 수행하였다. 이 모델에서는 전자와 이온에 대한 연속방정식을 풀어 시. 공간적으로 변하는 전하분포를 계산하고, 공간 전하 분포의 변화에 따라 결정되는 전기장은 푸아송 방정식을 풀어 얻는다. 연속 방정식은 FCT (Flux-corrected transport) 알고리즘과 FEM (Finite Element Method) 방법을 적용하여 수치적으로 다루어 졌다. 50Torr 압력에서의 질소 방전에 대한 시뮬레이션 결과는 중간 압력 질소 방전의 물리적 특성에 관한 상세한 이해를 제공한다. 또 계산 결과와 실험 결과[1]와의 비교는 정성적으로 잘 일치하는 것을 보여 준다.

중소형 무인기 브레이크 시스템용 복합형 지능재료펌프 설계 (Design of the Compound Smart Material Pump for Brake System of Small·Medium Size UAV)

  • 이종훈;황재혁;양지연;주용휘;배재성;권준용
    • 항공우주시스템공학회지
    • /
    • 제9권3호
    • /
    • pp.1-7
    • /
    • 2015
  • In this study, the design of compound smart materials hydraulic pump that can be applied to a small-medium size UAV having a limited space envelope and weight has been conducted. Compound Smart Material Pump(CSMP) proposed in this paper is composed of a pressurize pump and a flow pump for supplying the high pressure and fluid displacement to overcome the disadvantages of the piezoelectric actuator which has a small strain. Though this compound smart material pump has been designed as small size and lightweight as possible, it can sequentially supply the sufficient large flow rate and pressure required for the brake operation. For the design of CSMP, about 2,700 kg (6,000 lb) class fixed wing manned aircraft was selected. Based on the established requirements, the design of the CSMP have been done by strength, vibration, and fluid flow analysis.

강릉시 도시 경관 구조의 시계열적 변화 연구 (Structural Urban Landscape Changes over Time Series in Gangneung-Si)

  • 염정헌
    • 한국환경과학회지
    • /
    • 제30권10호
    • /
    • pp.779-787
    • /
    • 2021
  • This study analyzes structural landscape changes over a time-series for a small and medium-sized city, Gangneung-Si, based on area and distribution patterns, and according to the type of land cover. Among the types of land cover, the area ratio of urbanized areas increased by 2.02% in the late 2010s as compared to the late 1980s, while there was a decrease of 2.69% in farmland and 0.69% in grassland areas. On analyzing the changes in land cover use by applying the Fragstats program, it was confirmed that landscape changes in urban and management areas were relatively severe according to the Landscape Shape Index, Largest Patch Index, and Aggregation Index. A pattern of concentrated expansion was also found around certain areas. In particular, from the analysis, it was established that the proportion of urbanized area had considerably increased and that the extent of farmland damage to management areas, including planned management areas, was large. Additionally, the Total Core Area generally indicated a reduction in the core areas of farmland and forest within urban and management areas. A medium-sized city showed significant changes besides large cities in terms of landscape structure. The developmental pressure on management areas, in particular, was quite high.

Determinants for the Adoption of Electronic Commerce by Small and Medium-Sized Enterprises: An Empirical Study in Indonesia

  • ASWAR, Khoirul;ERMAWATI, Ermawati;WIRMAN, Wirman;WIGUNA, Meilda;HARIYANI, Eka
    • The Journal of Asian Finance, Economics and Business
    • /
    • 제8권7호
    • /
    • pp.333-339
    • /
    • 2021
  • The study seeks to identify the determinants of the adoption of e-commerce by small and medium-sized enterprises (SMEs) in developing countries, in our case, in Indonesia. The aim of this study is to examine the factors influencing e-commerce adoption. This study uses the method of quantitative data collection based on a questionnaire survey of SMEs in Indonesia. The research relies on Regional Project stipulations regarding Business Development in Indonesia, to capture businesses with a range of 5 to 100 employees that are classified as SMEs. This study randomly chose 100 SMEs in Indonesia from the IndoNetwork database. Partial least square (PLS) structural model data processing was used for path coefficients analysis. Structural equation modeling is applied in this study to analyze the determinant factors on the e-commerce adoption. The study findings reveal that four factors, namely, perceived benefits, compatibility, technology readiness, and government support, significantly influence the adoption of e-commerce, whereas customer/supplier pressure does not have influence. So, this study concludes that perceived benefits, compatibility, technology readiness, and government support had a significant and positive relationship with e-commerce adoption. Meanwhile, customer/supplier pressure had no effect on the e-commerce adoption of by Indonesia SMEs.