• Reproductive and Developmental Biology
• /
• 제37권4호
• /
• pp.213-217
• /
• 2013

The objective of this study was to investigate the effect of bacterial contamination on elapsed time after preservation on boar semen. Known numbers of Escherichia coli (E. coli) were inoculated to freshly ejaculated semen and sperm parameters such as viability, motility, agglutination, acrosome integrity and hypo-osmotic swelling test were performed during 7 days of liquid preservation. Semen samples were prepared using antibiotic free BTS extender and 4 different levels of E. coli were treated to semen with following concentrations; 3,000, 5,000, 7,000, 10,000 CFU/ml of sperms. Semen samples were preserved at $17^{\circ}C$ for 7 days in semen storage until analyzed. Aliquots were subjected to measure the sperm viability, motility and agglutination using computer assisted sperm analysis (CASA) system, acrosome integrity was performed using chlortetracycline (CTC) staining method and hypo-osmotic swelling test was performed using hypotonic solution from day 1 (day of semen collection) to 7. Detrimental effects on sperm motility and viability were observed 3 days after preservation at the level of 5,000 CFU/ml (p<0.05). Percentage of sperm abnormality was higher (p<0.05) in over 5,000 CFU/ml groups. Sperm agglutination rate was also significantly higher (p<0.05) in groups of 5,000 and 7,000 CFU/ml. The rate of acrosome reacted sperm was higher as preservation time goes in all the samples but the pattern was clearly higher among E. coli contaminated groups (p<0.05). The sperm membrane integrity in terms of hypo-osmotic test, E. coli affects little compared to other sperm parameters. The deleterious effects observed due to the bacterial contamination in semen suggest that importance of hygiene protocol to minimize the bacterial contamination during semen collection and processing.

• 한국식품저장유통학회지
• /
• 제31권1호
• /
• pp.1-14
• /
• 2024

The utilization of coatings composed of bio-based materials in the processing and preservation of meat presents an environmentally conscious, secure, cost-effective, and superior method for prolonging the storage life of meat while also preserving its nutritional value. In this study, changes in physical, chemical, and microbiological characteristics of freshly cut beef coated with distilled water (control) and keratin-starch composites (K-S) functionalized with 0.0-, 0.2-, 0.6-, and 1.0-mL avocado peel polyphenolic-rich extract (APPPE) kept at 4℃ for 12 days were evaluated periodically at 3-day interval using standard techniques. Keratin was extracted from waste feathers, while starch was obtained from ginger rhizomes. Following a 12-day storage period, beef coated with APPPE-enriched K-S composites exhibited a significant (p<0.05) improvement in shelf life by minimizing deteriorative changes in pH and color (as determined by metmyoglobin level) in addition to inhibiting oxidative changes in lipids (as determined by TBARS level) and proteins (protein carbonyl level) in comparison to control and K-S composite without APPPE. Furthermore, microbial growth was significantly (p<0.05) suppressed in meat coated with K-S composite functionalized with APE at 0.6 and 1.0 mL compared to the control. The study suggested that APPPE-enriched K-S composite could offer an eco-friendly and safe food preservation technique for fresh meat.

• 한국전자현미경학회:학술대회논문집
• /
• 한국현미경학회 2005년도 제36차 춘계학술대회 및 제3회 HVEM 이용자 워크샵
• /
• pp.47-48
• /
• 2005

• 한국항공우주학회지
• /
• 제32권8호
• /
• pp.12-19
• /
• 2004

미소평판 주위의 저속 기체유동장이 기체분자운동론에 근거한 방법으로 조사되었다. 모델충돌적분항으로 단순화된 볼츠만방정식을 Discrete Ordinate 방법과 결합된 유한차분법으로 수치해석 하여 길이가 $20{\mu}m$인 5% 평판 주위의 유동장을 계산하였다. 계산결과가 Information Preservation 방법 및 미끄럼 경계조건을 이용한 연속체 방법에 의한 결과와 비교되었다. 세 가지 서로 다른 방법에 의한 계산결과가 기본적으로 유사한 유동형태를 예측하였으나, 세부적인 변에서는 본 방법의 결과가 다른 두 방법의 결과보다 더 정확함을 보였다.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• 제15권8호
• /
• pp.2749-2763
• /
• 2021

The desirable result of infrared (IR) and visible (VIS) image fusion should have textural details from VIS images and salient targets from IR images. However, detail information in the dark regions of VIS image has low contrast and blurry edges, resulting in performance degradation in image fusion. To resolve the troubles of fuzzy details in dark regions of VIS image fusion, we have proposed a method of reflectance estimation for IR and VIS image fusion. In order to maintain and enhance details in these dark regions, dark region approximation (DRA) is proposed to optimize the Retinex model. With the improved Retinex model based on DRA, quasi-Newton method is adopted to estimate the reflectance of a VIS image. The final fusion outcome is obtained by fusing the DRA-based reflectance of VIS image with IR image. Our method could simultaneously retain the low visibility details in VIS images and the high contrast targets in IR images. Experiment statistic shows that compared to some advanced approaches, the proposed method has superiority on detail preservation and visual quality.

• 한국식품저장유통학회지
• /
• 제30권3호
• /
• pp.383-394
• /
• 2023

The ultra-fast quantitative real-time polymerase chain reaction (qPCR) assay was developed and validated to differentiate the morphologically similar ones, Oncorhynchus keta and Oncorhynchus mykiss. Species-specific primers were designed for the COI genes of mtDNA. The species-specific primers designed for O. keta and O. mykiss were selectively amplified by O. keta and O. mykiss DNA, respectively. The sensitivity of O. keta and O. mykiss primers was 1 ng/μL. Quantitative testing showed that the results met the 'Guidelines on Standard Procedures for Preparing Analysis Method such as Food' proposed by the Ministry of Food and Drug Safety. The qPCR method developed and validated in this study for identifying O. keta and O. mykiss has advantages such as speed and field applicability. Therefore, this method is expected to help control forgery and alteration of raw materials in the seafood industry.

• 정보처리학회논문지:소프트웨어 및 데이터공학
• /
• 제3권10호
• /
• pp.429-440
• /
• 2014

홍채 인식은 인간의 눈 영상으로부터 고유한 홍채특징을 추출하고 이를 코드화 하여 비교하는 생체인식 기술로, 이것은 시스템 안에 저장된 다른 홍채들과의 비교기술을 포함한다. 한편, 홍채 영상에서의 속눈썹은 인식률에 영향을 미치는 외부 요소인데, 만일 속눈썹이 홍채영역으로부터 정확하게 제거되지 않는다면 속눈썹을 홍채특징으로 인식하거나 홍채특징을 속눈썹으로 인식하는 오인식의 문제가 존재하게 되며, 결국 이 오인식은 홍채정보의 많은 유실을 가져오게 된다. 따라서 본 논문에서는 이러한 문제점을 해결하기 위하여 주파수 특징 분석에 사용되는 Gabor Filter를 이용한 속눈썹 제거로 홍채정보의 보존율 향상을 가져올 수 있었다. Gabor Filter는 영상의 주파수 분석을 위한 필터 중 하나인데 여기에 각도, 주파수, 가우시안 파라미터 등을 이용한 다양한 홍채영역의 특징들을 추출할 수 있는 새로운 방법으로 다양한 길이와 모양의 속눈썹을 정확하게 제거할 수 있었다. 그 결과 제안한 방법은 GMM 혹은 히스토그램 분석을 이용한 기존 방법보다 홍채영역 데이터 보존율에 있어서 약 4% 정도의 향상이 가능하였다.

• 전자공학회논문지S
• /
• 제35S권1호
• /
• pp.141-148
• /
• 1998

We propose an efficient method of de-interlacing in the post-pocessing of HDTV signal. Since the proposed method considers human visual weight, it gives the better performance in the edge information preservation than that of the median filter. The processing time of the proposed method is twice faster than median filter, therefore the proposed method is suitable for the real-time de-interlacing in the post-processing of HDTV signal.

• Restorative Dentistry and Endodontics
• /
• 제34권4호
• /
• pp.356-363
• /
• 2009

본 연구의 목적은 흰 쥐의 상악 대구치를 발거한 후 치주인대세포를 압력을 이용한 저속 냉동 보관법으로 냉동 보관시 치주인대세포의 활성도를 저속 냉동법, 냉장 보관법과 비교하여 평가하고자 하였다. 생후 4주된 암컷 Sprague-Dawley계 흰쥐의 상악 좌우 제 1,2 대구치를 발거하여 각 군 당 18개의 쥐 치아를 MTT, WST-1 검색에 이용하였다. 실험군은 4개군으로 대조군은 즉시 발치군이며, $4^{\circ}C$냉장고에 일주일간 보관한 냉장군, 발치 후 동해방지제 처리과정을 거쳐 $4^{\circ}C$에서 $-35^{\circ}C$까지 $-0.5^{\circ}C$/min 속도로 서서히 냉동시킨 뒤 $-196^{\circ}C$에 냉동한 저속 냉동군, 3MPa의 압력을 가하고 $-0.5^{\circ}C$/min 속도로 $4^{\circ}C$에서 $-35^{\circ}C$까지 서서히 냉동시킨 뒤 $-196^{\circ}C$에 냉동한 압력 저속 냉동군으로 나누었다. 보존액은 F medium을 사용했으며 동해방지제로 10% dimethylsulfoxide(DMSO)를 사용하였다. 일주일 후 해동하여 MTT, WST-1검색을 시행한 뒤 치근면을 단위면적으로 표준화하기 위해 MTT, WST-1 측정값을 Eosin 염색 후 530nm에서 측정한 흡광도 값으로 나누었다. 통계 분석을 위해 one way ANOVA를 시행하였으며 사후 검정으로는 Tukey 방법을 사용하였고 결과는 다음과 같다. 1. MTT 검색에 의한 흡광도를 Eosin 염색 후 측정한 흡광도로 나눈 값에서는 압력 저속 냉동군은 즉시 발치군보다 유의성 있게 세포 활성도가 낮았으나(p<0.05), 저속 냉동군이나 냉장군과 비교할 때는 높은 세포 활성도를 보이며 통계적 유의성이 있었다(p<0.05). 2. WST-1 검색에 의한 흡광도를 Eosin 염색 후 측정한 흡광도로 나눈 값에서도 MTT 검색과 마찬가지로 압력 저속 냉동군에서는 즉시 발치군보다 유의성 있게 세포 활성도가 낮았으나(p<0.05), 저속 냉동군이나 냉장군과 비교할때는 높은 세포 활성도를 보이며 통계적 유의성이 있었다(p<0.05). 위의 결과를 통해, 3MPa(30기압) 압력을 이용한 저속 냉동 보관법은 $4^{\circ}C$냉장법이나 압력을 사용하지 않은 다른 냉동 보관법에 비해 우수한 쥐 치아의 치주인대세포의 활성도를 보여 차후 치아의 재식/이식을 위한 중-장기 치아보관을 위한 방법으로의 가능성을 제시하였다.

• 한국수정란이식학회지
• /
• 제28권2호
• /
• pp.121-125
• /
• 2013

This study was carried out to investigate the general characteristics of semen such as semen volume, pH, sperm motility and sperm concentration of the semen collected from Shih Tzu dogs (age of 24 to 48 months, weight of 4 to 8 kg) by using the method of digital manipulation of the penis. The effect of preservation temperature and time on motility of fresh semen was also investigated in the present study. Semen was collected for 16 times from 4 male Shih Tzu dogs by multiple ejaculations (four times ejaculation per dog). The average of semen volume, semen pH, sperm motility and sperm concentration of the second fraction containing small volume of the initial third fraction per ejaculation were $2.11{\pm}0.31$ ml, $6.25{\pm}0.07$, $97.59{\pm}1.03%$ and $2.05{\pm}0.14{\times}10^8$ cells/ml, respectively. Average semen volume per ejaculate, semen pH, sperm motility and sperm concentration of the first fraction from the ejaculation were $1.12{\pm}0.15$ ml, $5.99{\pm}0.14$, $16.09{\pm}6.18%$ and $5.16{\pm}2.03{\times}10^5$ cells/ml, respectively. Those of second fraction were $2.07{\pm}0.29$ ml, $6.36{\pm}0.13$, $97.31{\pm}1.36%$ and $2.15{\pm}0.30{\times}10^8$ cells/ml, respectively. Those of third fraction were $2.60{\pm}0.29$ ml, $6.63{\pm}0.08$, $95.72{\pm}1.61%$ and $6.03{\pm}1.83{\times}10^7$ cells/ml, respectively. Sperm motility was significantly higher at $17^{\circ}C$ preservation temperature than at $5^{\circ}C$ or $36^{\circ}C$ during preservation period except 1 h preservation (P<0.05). When preservation temperature was $17^{\circ}C$, sperm motility was $96.69{\pm}1.49%$ at 1 h, $91.38{\pm}1.90%$ at 6 h, $88.38{\pm}2.34%$ at 12 h, $78.13{\pm}4.58%$ at 18 h, $58.44{\pm}8.57%$ at 24 h and $29.56{\pm}5.06%$ at 30 h, respectively.