• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2003.05a
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• pp.148-148
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• 2003

This study is to investigate the environmental levels and trend of dioxins, which was the 3$\^$rd/ year of environmental monitoring research for endocrine disrupting chemicals since 1999. Total 282 samples were analyzed from 115 sites including 26 sites of airs, 43 sites of waters, 11 sites of sediments and 35 sites of soil, which were the same as those of investigated sites in 2000. Sampling period was from June 2001 to June 2002. Target chemicals were seventeen species of 2,3,7,8-chlorine-substituted PCDD and PCDF congeners and were analyzed by the standard methods, established by National Institute Environmental Research (NIER). The average concentration of dioxins in air decreased from 0.324 pg-TEQ/N㎥ in 2000 to 0.287 pg-TEQ/N㎥ in 2001, and those in water and soil were 0.073pg-TEQ/L and 1.703pg-TEQ/dry g, respectively, which was the less values detected in 2000. In sediment, however, the value was 0.086pg-TEQ/dry g, which was the increase from the value of the year 2000. The concentration range of dioxins in air for 26 sites in 17 regions detected were 0.013∼l.664pg-TEQ/N㎥, 4 sites from those were exceeded the Air Quality Standards of Dioxin in Japan (0.6 pg-TEQ/N㎥). The tolerable daily intake of dioxins was calculated at the highest level (1.664) in air, with referring the soil and food data from Japan, was calculated to be 2.85pg-TEQ/kg/day, which was below the level of 4 pg-TEQ/kg/day suggested in KFDA(Korea). While the average concentration of dioxins in 15 big cities was 0.190 pg-TEQ/N㎥, that in 8 medium/small cities constituting an industrial complex was 0.558 pg-TEQ/N㎥. In water, the concentration range detected were 0∼0.946pg-TEQ/L and the trend of the average concentrations shows an increase from those of 1999 but decreased from those of 2000, any sites however were not exceeded the Water Quality Standards of Dioxin in Japan (1 pg- TEQ/L). In soil. the detected range were 0∼43.333 pg-TEQ/dry g and the average concentration decreased, compared with the results of 2000. According to the monitoring results by land utilization, the detected range were 0∼43.333pg-TEQ/dry g in farmland, 0.017∼0.601 pg-TEQ/dry g in the industrial area, 0.005∼0.049pg-TEQ/dry g in the park and 0.008∼1.825 pg-TEQ/dry g in the rest. In sediment, the detected range increased from 0∼0.244 pg-TEQ/dry g to 0∼0.537 pg-TEQ/dry g, based on the results of 2000. For the proper control of dioxins, continuous monitoring needs to be performed and in addition, the dioxin inventory should be prepared for major sources through the dioxin emission survey. These results would provide sound and solid basis for proper decision making of dioxins management like establishment of environmental quality standards in Korea.

• Applied Biological Chemistry
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• v.44 no.3
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• pp.185-190
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• 2001

Alginate, a well-known biopolymer, is universally applied for immobilization of microbial cells. Biosorption characteristics of lead by waste biomass of immobilized A. niger beads, used in fermentation industries to produce citric acid, were studied. The immobilized A. niger beads, prepared via capillary extrusion method using calcium chloride, were applied in the removal of lead. Pb uptake was the highest in A. niger beads cells grown for 3 days with medium producing citric acid (12% sucrose, 0.5% $NH_4NO_3$, 0.1% $KH_2PO_4$, and 0.025% $MgSO_4$). Lead uptake by the immobilized A. niger beads and free A. niger mycellia beads increased sharply with time. However, while uptake by the immobilized A. niger beads continued to increase slowly, that by free A. niger mycellia beads stopped after 30 min. The optimum pH and temperature of lead uptake were found to be 6 and $35^{\circ}C$, respectively. The maximum uptake of lead was achieved with $50{\sim}100$ beads and 50 ml lead solution in a 250-ml Erlenmeyer flask, while, at over 100 beads, uptake of the lead decreased. The order of biosorption capacity for heavy metals was Pb>Cu>Cd. Pb uptake capacity of the immobilized A. niger beads treated with 0.1 M $CaCI_2$, 0.1 M NaOH, and 0.1 M KOH decreased compared to the untreated beads. On testing the desorption of Pb from the immobilized A. niger beads, re-uptake of Pb was found possible after desorption of the binding metal with 0.1 M HCI.

• Journal of Applied Biological Chemistry
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• v.61 no.2
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• pp.125-130
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• 2018

The quality and microbiological characteristics of various Mul-kimchi prepared with addition of 0.02, 0.05, and 0.1% (w/v) chlorella water extract (CWE) were investigated during fermentation for 7 days at $10^{\circ}C$. The addition of CWE powder stimulated the growth of lactic acid bacteria and considerably improved the acid production. After 6 days fermentation, titratable acidity of CWE Mul-kimchi was 0.16-0.19% and was higher than that (0.14%) of Mul-kimchi made without CWE. The lactic acid bacterial counts were increased rapidly in CWE Mul-kimchi during fermentation for 4 days. The sensory quality of Mul-kimchi added with 0.05-0.1% CWE powder lightly enhanced in taste and overall acceptability among the tested Mul-kimchis preparations. When CWE Mul-kimchi preparations fermented for 6 days were incubated at $4^{\circ}C$ for 15 days, their titratable acidities were continuously increased up to 0.21-0.26% but number of viable lactic acid bacteria were well maintained above $10^7CFU/g$ through storage period.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.2
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• pp.153-157
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• 2000

Hydrolysate which inhibit the Angiotensin I-converting enzyme (ACE) was prepared from mackerel muscle by pretense. The ACE inhibitory activity of mackerel muscle hvdrolysate (MMH) was $967 {\mu}g of IC_(50)$. ACE inhibitory peptides were isolated by ultrafiltration, gel permeation column chromatography (GPC), reversed phase column chromatography(RPC), reversed phasehigh performance liquid chromatography (RP-HPLC), and gel permeation high performance liquid chromatography (GP-HPLC) from the MMH. The amino acid sequence of the ACE inhibitory peptides was Tyr-Val-Ala. The $IC_(50)$ of this peptide for ACE from rabbit lung was $1.4 {\mu}M$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.4
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• pp.285-292
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• 2010

To prepare high-quality fast foods using salmon, this study developed a docosahexaenoic acid (DHA)-enriched salmon patty, and compared its quality with soybean oil-added patty and commercial patty. The moisture and lipid contents of the DHA-enriched patty were 61.9% and 7.7%, respectively. The moisture content was lower than in the commercial patty, while the lipid content was higher. The lipophilic and hydrophilic browning indices of the DHA-enriched patty were 0.10 and 0.05, respectively, which were lower than those of the commercial patty. The DHA-enriched patty had 5% less trichloroacetic acid (TCA)-soluble nitrogen than the commercial patty, and it was firmer. There were no differences in the proximate composition, browning indices, TCA-soluble nitrogen content, and hardness between the soybean-added and DHA-enriched patties prepared in this experiment. Examining the fatty acid compositions of the patties, the saturated acid and monoenoic acid compositions decreased in the following order: DHA-enriched patty > soybean oil-added patty > commercial patty, while the polyenoic acid composition increased in that order. The total amino acid content of the DHA-enriched patty was 19.13 g/100 g, which was 32% higher than that of the commercial patty (14.47 g/100 g). No enriching effect of minerals would be expected on eating a 100 g DHA-enriched patty, except for phosphorus. The sensory evaluation indicated that the DHA-enriched patty was superior in color and taste to the commercial patty, while there was no difference in flavor between the patties.

• Microbiology and Biotechnology Letters
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• v.30 no.2
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• pp.177-183
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• 2002

The extracts from approximately 40 different Korean traditional medicines were prepared to investigate the antimicrobial activities against poultry disease-related bacteria. Among tested, the extracts of Schizandra chinensis (SC), Melia azedarach (MA), Caesalpinia sappan (CS) and Rhus javanica (RJ) exhibited significant antimicrobial activities against Salmonella gallinarum, whereas the extracts of Elsholtzia ciliata (EC), Myristica fragrans (MF), Alpinia katsumadai (AK), Poncirus trifoliata (PT), Prunella vulgaris (PV), CS and RJ exhibited antimicrobial activities against Staphylococcus aureus. Minimum inhibitory concentrations (MIC) of MA, CS and RJ extracts against S. gallinarum were 1.2 mg/ml, whereas MIC of RJ extract for S. aureus was 0.6 ㎎/ml, which was the lowest among tested. The antimicrobial activities of SC and RJ extracts against S. gallinarum were reduced, but those of AK and CS extracts against S. aureus were not affected by heating treatment. The antimicrobial activities of SC extract against S. gallinarum and those of EC, PT and RJ extracts against S. aureus were stable by acid treatment but unstable by alkaline treatment. those of CS extract was not effected by either acid or alkaline treatment. The growth of all bacteria was significantly inhibited within 24 hours by the addition of at least 100 ppm and 300 ppm of RJ and CS extracts, respectively, compared with the control group. In conclusion, these findings suggest that RJ and CS extracts may play important roles for antimicrobial activities against poultry disease-related bacteria.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.3
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• pp.231-236
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• 2002

Encapsulating 1-octen-3-ol with alginates solution, the effects of physical properties (viscosity, emulsion activity, emulsion stability) of alginates solutin on the retention of 1-octen-3-ol in capsules were investigated. Only alginates solutions haying less than 350 cP in viscosity were capable to be adopted to spray dry. Adding citric acid to alginates solution in order to reduce its viscosity, the concentration of citric acid became higher, the viscosity of alginates solution were lower. Adding $0.1\% of citric acid could reduce viscosity of alginates solution to 150 cP. The viscosity of alginates solution after emulsifying showed higher value than that of solution before emulsifying, but its viscosity were within the possible ranges for spray drying. The lower viscosity of alginates solution were, EAI became higher but ESI and amount of remaining 1-octen-3-ol in capsules were lower, In reducing the viscosity of alginates solutions, heating time after adding citric acid were longer, the their viscosity became lower. Differences of viscosity of alginates solution after and before emulsifying were little, In encapsulating raw pine agaric with alginates solution, the adding amount of soybean oil increased, the amounts of remaining 1-octen-3-ol in capsules increased. After freeze drying the amount of remaining 1-octen-3-ol in alginates capsules prepared with raw pine agaric was higher than that after cold air flow drying.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.3
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• pp.368-381
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• 2020

This study was conducted to optimize the processing conditions of seasoned laver Pyropia yezoensis with conger eel Conger myriaster seasoning sauce (CES) using response surface methodology (RSM). The RSM program results for bonesoftness showed that the optimum independent variables based on the dependent variables (Y₁, lipid removal rate; Y₂, texture; and Y₃, sensory fish odor score) were 431.0% for X₁ (water amount), 115.6℃ for X₂ (retort-operated temperature), and 50.1 min for X₃ (retort-operated time). The RSM program results for the CES blend showed that the optimum independent variables (X₁, amount of bone-softened conger eel by-products; X₂, mixed sauce amount; and X₃, starch amount) based on the dependent variables (Y₁, amino-N; Y₂, Hunter redness; and Y₃, drying time) were 44.8% for A (pre-treated conger eel by-product), 36.0% for B (mixed sauce), and 19.2% for C (starch). The RSM program results for seasoned laver with CES showed that the optimum independent variables based on the dependent variables (Y₁, water activity; Y₂, Hunter yellowness; and Y₃, overall acceptance) were 5.0% for X₁, (CES amount), 313.8℃ for X₂ (roasting temperature), and 6.0 s for X₃ (roasting time). The seasoned laver with CES prepared under the optimum conditions was superior to commercial seasoned laver in terms of overall acceptance.

• Journal of Sericultural and Entomological Science
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• v.48 no.1
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• pp.6-10
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• 2006

Recently, B. mori proteins such as silk fibroin and silk sericin have been found to have a water-holding capacity, anti hydrogen peroxide toxicity, antioxidant activity and tyrosinase-inhibitory activity (Yeo 2006, Kurioka 1999 & 2004), implying its potential usefulness of the application for cosmetic and functional food(Yamazaki 1999 & Une 2000). We are tried to application for dietary resources of B. mori silk fibroin and sericin that were prepared to some of different molecular cutting these resources by preparative recycling HPLC system. In our studies with rats have demonstrated that consumption of these silk proteins are being prevents constipation effect and it is maybe enhances intestinal absorption of water and dietary effects. These some of useful results further suggest a usefulness of sericin as dietary resources for health.

• Korean Journal of Microbiology
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• v.51 no.4
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• pp.419-426
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• 2015

The aims of this study were to isolate spore-forming Bacillus strains that exhibit high digestibility and anti-pathogenic bacteria toward feed for calves. Total 136 spore-forming strains were isolated from finished feeds and their ingredients. Among them, 93 strains were identified as Bacillus species when analyzed by 16S rRNA sequencing. For industrial use, three strains named as Bacillus licheniformis SHS14, B. subtilis LCB7, B. amyloliquefaciens LCB10 were selected after evaluating the industrial standards that are related with heat and acid resistance, enzyme activities, and anti-pathogenic activities against Samonella dublin ATCC15480 and E. coli K99. After each culture, 3 selected strains were mixed together at 1:1:1 (v/v/v) ratio and then prepared as the mixed starter culture for feeding. The changes in microbial community were analyzed via 16S rRNA metagenomics. The initial community ratio among three strains was maintained even after manufacturing into final products. Also, in vitro, enzymatic and anti-pathogenic activities were almost same as those when cultured in single culture, and results of anti-pathogenic activities conducted with calves showed 90% activities against lincomycin, which would be indicative of a promising feed starter.