• 제목/요약/키워드: Preliminary experiment

검색결과 582건 처리시간 0.028초

Penicillium islandicum에 의한 Skyrin의 생합성 (Biosynthesis of Skyrin in Penicillium islandicum)

  • 조성환;죤 에이 앤더슨
    • Applied Biological Chemistry
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    • 제27권3호
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    • pp.151-157
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    • 1984
  • Penicillium islandicum은 Czapek-Dox 배지를 이용하여 어두운 곳에서 배양되었을때 황적색 색소인 skyrin을 생합성 하였다. $[^{14}C]$-acetate 배지에 이 균주를 접종 배양하여 얻어진 대사산물로부터 radioactive skyrin을 순수 정제 분리할 수 있었고 이를 sodium dithionite 처리하여 Emodin이 합성되는 것을 동정할 수 있었다. 한편, Penicillium islandicum의 배양액에서 추출 정제된 효소를 이용한 균체의 효소실험에서 $[^3H]$-emodin과 $[^3H]$-emodin은 skyrin생합성의 유력한 전구체물질임이 밝혀졌으며, 효소반응결과 $[^3H]$ emodinanthrone(4.8%)이 $[^3H]$-emodin보다 radioactive skyrin으로 주입되는 비율이 더 크다는 것을 알 수 있었다. 균체외 효소실험결과, skyrin이 생합성되는 경로는 $emodinanthrone{\rightarrow}emodin{\rightarrow}skyrin$으로 진행되었다.

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BIM 설계를 위한 커리큘럼 연구 - 초등학교 설계실험 - (A study of Curriculum for BIM Design - On the Design Exeperiment of the Elementary School -)

  • 김용일;양관목
    • 교육시설 논문지
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    • 제23권6호
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    • pp.11-17
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    • 2016
  • The objective of this paper is to introduce a curriculum for BIM design and explores characteristic of BIM Design. In order to get our approach, conducted by means of BIM throughout an actual experiment by elementary school from Architectural programming, preliminary design to design development and construction drawing. The experiment states that curriculum for BIM design requires systemically to be made in the architectural education. And also schools integrate BIM teaching early into in their curriculum. Some schools claim that BIM is often perceived as stumbling block student' creativity on account of its instant building elements. But this is not so; it all depends on how BIM is taught in the architectural education. The results state that students in the architectural school today is required to learn design by means of BIM early in the first year. Also more importantly, in order to advance the BIM design, a curriculum for BIM design is required to diversely open class in the architectural education.

A Study on the CDMA-based TT&C Design and Experiment Concept

  • Lee, Ho-Jin;Mo, Hee-Sook
    • 제어로봇시스템학회:학술대회논문집
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    • 제어로봇시스템학회 1999년도 제14차 학술회의논문집
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    • pp.37-40
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    • 1999
  • ETRI has successfully completed and delivered to KARI the KOMPSAT Mission Control System. This system was designed to work in the conventional TT&C modulation scheme with the pre-assigned frequencies. As a way to accelerate in catching up with future TT&C technology evolutions, a preliminary study needs to be carried out to prepare for the development of a spread spectrum applicable to TT&C. A brief study was carried out to review some points to be considered in designing and implementing spread spectrum schemes to the ground TT&C system intended for a LEO spacecraft. Also a simulation and link design revisit was performed to see the operational and technical benefits with the KOMPSAT TT&C parameters. An experiment concept is proposed to test as many functions at a time once the prototype is developed. In this configuration, a ground-model TT&C transponder is connected via LAN to the ETRI-developed KOMPSAT S/W simulator and linked to the KOMPSAT TM/TC processing s/w via spread spectrum signals through a GEO satellite bent-pipe link. A satellite data relay link simulation could be carried out in this configuration.

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Mathematical Planning for Revealing Optimal Synthetic Conditions of Naphthalene Chloromethylation

  • Pak, V.V.;Karimov, R.K.;Shakhidoyatov, Kh.M.;Yun, L.M.;Soh, D.W.
    • 동굴
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    • 제71호
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    • pp.1-4
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    • 2006
  • Chloromethylnaphthalene is a valuable compound for obtaining of the plant growing stimulator - -napthylacetic acid. Chloromethylation of naphthalene by paraformaldehyde in the presence of glacial acetic acid, phosphoric and hydrochloric acids at temperature 80 - 85C and duration - 6 hours the -chloromethylnaphthalene yield was 55-57%. Using Box-Wilson method for mathematical planning of experiment carried out optimization of its synthesis for purpose increasing -chloromethylnaphthalene yield. Preliminary, one - factor experiments were carried out for selecting independence main parameters influencing on the synthesis. A full factor experiment of 23 with extended matrix of planning was used for optimization. Aiming to increase the -chloromethylnaphthalene yield, the obtained mathematical model was used for program of sharp raising on the reply surface. The received optimal conditions for the -chloromethylnaphthalene synthesis were selected as following: molar ratio of naphthalene parapfsormaldehyde of 1 : 2 temperature - 105C duration of the reaction - 3 hours. The yield of -chloromethylnaphthalene under these optimal conditions was 75%.

The MARS Simulation of the ATLAS Main Steam Line Break Experiment

  • Ha, Tae Wook;Yun, Byong Jo;Jeong, Jae Jun
    • 에너지공학
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    • 제23권4호
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    • pp.112-122
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    • 2014
  • A main steam line break (MSLB) test at the ATLAS facility was simulated using the best-estimate thermal-hydraulic system code, MARS-KS. This has been performed as an activity at the third domestic standard problem for code benchmark (DSP-03) that has been organized by Korea Atomic Energy Research Institute (KAERI). The results of the MSLB experiment and the MARS input data prepared for the previous DSP-02 using the ATLAS facility were provided to participants. The preliminary MSLB simulation using the base input data, however, showed unphysical results in the primary-to-secondary heat transfer. To resolve the problems, some improvements were implemented in the MARS input modelling. These include the use of fine meshes for the bottom region of the steam generator secondary side and proper thermal-hydraulics calculation options. Other input model improvements in the heat loss and the flow restrictor models were also made and the results were investigated in detail. From the results of simulations, the limitations and further improvement areas of the MARS code were identified.

교차로 인지와 방향지시등 조작 지점에 관한 검토 (Position of Intersection Recognition and Tum Signal Operation Approaching at Target Intersection)

  • 전용욱
    • 한국안전학회지
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    • 제24권3호
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    • pp.65-70
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    • 2009
  • In-vehicle route guidance information(RGI) systems have been developed with the advancement of the information and communication technologies. However, the RGI is provided by a pre-determined option, drivers occasionally pass the target intersection owing to non- or late- recognizing it. The purpose of this experiment is to examine the position of driver's tum signal operation and intersection recognition approaching at the target intersection which is difficult to identify as a preliminary research on developing the additional RGI connecting with the tum signal control. The field experiment was conducted to measure distances of the turn signal operation and intersection recognition from the target intersection according to driving lanes and landmarks at adjacent intersection. And, glance behavior to the car navigation display was evaluated by using an eye camera. The results indicate that drivers operate the turn signal after confirming a landmark in the case of the intersection with it. However, most case of driving, drivers operate the tum signal at 40 to 50m before coming to the target. To provide the additional RGI, when drivers do not operate the tum signal approaching at the target intersection based on the results, is expected to improve the traffic safety and the comfort for drivers.

Study on Heat Generation of a Bulk HTS for Application to a 100 kWh SFES Superconductor Bearing

  • Jung, S.Y.;Lee, J.P.;Han, Y.H.;Han, S.C.;Jeong, N.H.;Ko, J.S.;Jeong, S.K.;Sung, T.H.
    • Progress in Superconductivity
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    • 제8권1호
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    • pp.122-126
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    • 2006
  • This paper presents experimental and numerical investigation on heat generation of a bulk HTS for application to a 100 kWh Superconductor Flywheel Energy Storage System(SFES) bearing. An experimental device is manufactured to reproduce varying magnetic field conditions that a bulk HTS may experience during the operation of the 100 kWh SFES. The bulk HTS is directly cooled by a cryocooler while the heat is generated by the eddy currents created by varying magnetic fields induced by a coil. In order to design the cryocooling system for the 100 kWh SFES project, a preliminary experiment to investigate the actual cooling load variation under AC magnetic field has been carried out. In the experiment, two different copper holders were designed and tested. Several temperature sensors were installed on each component of the assembly and the temperatures were measured for several operating conditions of the 100 kWh SFES. The experimental investigation on the thermal response of the bulk HTS and its holder is considered to be a valuable step fur the successful materialization of a large-scale SFES.

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Control Effects of an Antibiotic Produced by Streptomyces sp. B25 on Tobacco Mosaic Virus and Determination of Its Molecular Structure

  • Yeo, Woon-Hyung;Kim, Young-Ho;Kim, Young-Sook;Kim, Sang-Seock;Chae, Soon-Yong
    • 한국연초학회지
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    • 제20권2호
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    • pp.172-177
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    • 1998
  • The culture filtrate of Streptomyces sp. B25, which was identified in this experiment, was tested for the control of tobacco mosaic virus (TMV) with the susceptible tobacco cultivar, NC 82, under the field conditions following the preliminary examination of its characters for TMV control. Control efficacy of the culture filtrate against TMV infection continued over 50% up to 6 days after treatment, and its systemic effect was about 30% of the direct effect. In field conditions control efficacy of the culture filtrate against TMV infection was 95.3 % at 2 weeks after TMV inoculation, and decreased to 58.3 % at 3 weeks after inoculation. Five fold-dilution of the culture filtrate showed about half of the control efficacy by the stock culture filtrate. Analysis of the antibiotic material responsible for the inhibition of TMV infection through nuclear magnetic resonance experiments revealed that the antibiotic is antimycin $A_1$, which is firstly reported as an anti-phytoviral antibiotic in this experiment.

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소형 터보홴 설계인자와 음질의 상관관계에 의한 설계 최적화 (Design Optimization by the Correlation between the Design Parameter and the Sound Quality of Small Turbo-fan)

  • 김휘중;정용규;이정수;이승배
    • 한국소음진동공학회논문집
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    • 제16권5호
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    • pp.485-494
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    • 2006
  • The state-of-the-art of low-noise fan design usually includes the consideration of optimal sound level and sound quality. The influential design parameters of the noise level by the centrifugal fan were selected based on the preliminary test. The centrifugal fans were designed according to the experiment plan method by specifying the selected design parameters. The experiment with these machined mock-up's of centrifugal impellers suggested the major design parameters among many, having impacts upon the indices of sound quality (e.g. loudness, sharpness, roughness and fluctuation strength) at the same operation point. With the response surface method, the major design parameters selected thereafter were analyzed to estimate each contribution upon the sound quality of the centrifugal fan, and the optimal values were drawn by the consideration of the sound quality levels and their regression equations. In addition, the validity of the regression equations was numerically verified by means of the coefficient of determination. Furthermore, the mechanism by which the centrifugal fan impeller influences the determinants of its sound quality was suggested.

Systemic Statistical Optimization of Astaxanthin Inducing Methods in Haematococcus pluvialis cells -Statistical Optimization of Astaxanthin Production in Haematococcus

  • Kim, Sun-Hyoung;Jeong, Sung Eun;Hong, Seong-Joo;Lee, Choul-Gyun
    • 한국해양바이오학회지
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    • 제6권1호
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    • pp.31-40
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    • 2014
  • The production of astaxanthin in the microalga Haematococcus pluvialis has been investigated using a sequential methodology based on the application of two types of statistical designs. The employed preliminary experiment was a fractional factorial design $2^6$ in which the factors studied were: excessive irradiance and nitrate starvation, phosphate deficiency, acetate supplementation, salt stress, and elevated temperature. The experimental results indicate that the amount of astaxanthin accumulation in the cells can be enhanced by excessive irradiance and nitrate starvation whereas the other factors tested did not yield any enhancement. In the subsequent experiment, a central composite design was applied with four variables, light intensity, nitrate, phosphate, and acetate, at five levels each. The optimal conditions for the highest astaxanthin production were found to be $1040{\mu}E/(m^2{\cdot}s)$ light intensity, 0.04 g/L nitrate, 0.31 g/L phosphate, 0.05 g/L acetate concentration.