• Proceedings of the Korean Institute of Resources Recycling Conference
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• 2003.10a
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• pp.196-200
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• 2003

A novel prepurification method was developed aiming at increasing yield and purity, also reducing solvent usage for purification of paclitaxel. The use of a micelle and precipitation in the prepurification process allows for rapid separation of paclitaxel from interfering compounds and dramatically reduces solvent usage compared to alternative methodologies. The prepurification process serves to minimize the size and complexity of the HPLC operations for paclitaxel purification. The process is readily scalable to a pilot plant and eventually to a production environment where multikilogram quantities of material are expected to be produced. As much as possible, the process has been optimized to minimize solvent usage, complexity, and operating costs.

• Proceedings of the Membrane Society of Korea Conference
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• 1995.10a
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• pp.45-46
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• 1995

Generally, two different techniques have been employed for the precipitation of membranes from a polymer casting solution. In the first method, a precipitant is introduced from a vapor phase. In this case the precipitaion rate is very slow and a more or less symmetric structure is formed. In the second method, a precipitant is added to a casting solution by immersing the cast polymer film in a nonsolvent bath. In this case the precipitation rate is very fast and a skinned asymmetric membrane structure is obtained. In this study, we introduced water-vapor to PSf/NMP solution and took photographs of phase separation phenomena of polymer lean phase.

• Nuclear Engineering and Technology
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• v.55 no.2
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• pp.391-407
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• 2023

This article provides a survey of wet (aqueous) methods for recovery, separation, and purification of uranium from fission products in carbonate solutions during the reprocessing of spent nuclear fuel and methods for removal of radionuclides from alkaline radioactive waste. The main methods such as selective direct precipitation, ion exchange, and solvent extraction are considered. These methods were compared and evaluated for reprocessing of spent nuclear fuel in carbonate media according to novel alternative non-acidic methods and for treatment processes of alkaline radioactive waste.

• Membrane Journal
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• v.5 no.2
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• pp.74-80
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• 1995

Asymmetric poly(vinyl alcohol) (PVA) membranes were prepared by varying the precipitation conditions of the phase inversion technique, and the influences of precipitation conditions on the water uptake of the membrane were investigate. The degree of water uptake of the membrane increased and reached a certain maximum value, as the precipitation time increased. However, it decreded after that. As the precipition temperauure became lower, the degree of water uptake increased more or less. The addition of a nonionic surfactant to the precipitaon solution was effective reducing the precipitation time by lowering the surface tension of the precipitation solution, but ig didn't change the maximum value of its own degree of water uptake. In addition, the relationship between the degree of water uptake and the separation characteristics and mechanical properies of the membrane were investigated. The selectivity factor of the membrane for pervaperation separation decreased, but the permeate flux increased, respectively, with increasing the degree of water uptake. The tensile strength and elongation of the membrane, respectively, increased to a maximum value with increasing the degree of water uptake, and then decreased abtuptly.

• Journal of Microbiology and Biotechnology
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• v.12 no.3
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• pp.522-525
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• 2002

A very rapid and efficient separation technique for cellular rhizobial cyclosophoraoses was developed based on fractional precipitation and partition chromatography. Cyclosophoraoses are known to function in the osmotic regulation and root nodule formation of legumes during the nitrogen fixation process. Cyclosophoraoses are produced as unbranched cyclic (1longrightarrow12)-${\beta}$-D-glucans in Agrobacterium or Rhizobium species. Recent research has shown that cyclosophoraoses can form inclusion complexation with various unstable or insoluble guest chemicals, thereby implying great potential for industrial application. Typical separation of pure cellular cyclosophoraoses has been so far carried out by several time-consuming steps, including size exclusion, anion exchange, and desalting liquid chromatographies, with a relatively poor recovery. However, the proposed method demonstrated that the successive application of fractional ethanol precipitation and one step of silica gel-based flash column chromatography was enough to simultaneously purify neutral or anionic forms of cyclosophoraoses. This novel technique is very rapid and provides a high recovery.

• Macromolecular Research
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• v.9 no.5
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• pp.285-291
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• 2001

The polysulfone(PSf)/polyethersulfone(PES) blend membranes were prepared by an immersion precipitation method. N-methyl-2-pyrrolidone(NMP) was used as a solvent and water as a nonsolvent. The composition of the coagulation bath and the dope polymer concentration as well as the blend ratio of two polymers were varied. The membrane morphologies were interpreted on the basis of the phase diagram of the PSf/PES/NMP/water system. As the solvent content in the coagulation bath increased in the single polymer system, the number of macrovoids decreased and the morphology was changed from finger-like to cellular structure. In the given bath condition phase separation occurs earlier for the solutions of PSf/PES blend than for those of single polymer. A horizontally layered structure and horizontal protuberances inside the macrovoid were observed for the membranes formed from PSf/PES blend solutions. This peculiar structure formation can be interpreted by a PSf-rich/PES-rich phase separation followed by a polymer-rich/polymer-lean phase separation during the exchange of solvent and nonsolvent.

• Proceedings of the Korean Nuclear Society Conference
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• 2001.05a
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• pp.453.1-453
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• 2001

• Bulletin of the Korean Chemical Society
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• v.28 no.5
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• pp.721-722
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• 2007

• Macromolecular Research
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• v.11 no.1
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• pp.53-61
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• 2003

A molecular-thermodynamic model is developed for the salt-induced protein precipitation. The protein molecules interact through four intermolecular potentials. An equation of state is derived based on the statistical mechanical perturbation theory with the modified Chiew's equation for the fluid phase, Young's equation for the solid phase as the reference system and a perturbation based on the protein-protein effective two body potential. The equation of state provides an expression for the chemical potential of the protein. In a single protein system, the phase separation is represented by fluid-fluid equilibria. The precipitation behaviors are simulated with the partition coefficient at various salt concentrations and degree of pre-aggregation effect for the protein particles. In a binary protein system, we regard the system as a fluid-solid phase equilibrium. At equilibrium, we compute the reduced osmotic pressure-composition diagram in the diverse protein size difference and salt concentrations.

• Korean Chemical Engineering Research
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• v.54 no.1
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• pp.89-93
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• 2016

In this study, we investigated the separation behavior of the anticancer agent paclitaxel and its semi-synthetic precursor 10-deacetylpaclitaxel (10-DAP) from plant cell cultures. As a result of sequential separation/purification performed by biomass extraction with solvent, liquid-liquid extraction, adsorbent treatment, hexane precipitation, and fractional precipitation, the adsorbent treatment was found to be the most effective in separating and recovering 10-DAP from paclitaxel. The optimal adsorbent type, crude extract/adsorbent ratio, and adsorbent treatment temperature were sylopute, 1:1.5 (w/w), and $20^{\circ}C$, respectively. The separation/recovery of 10-DAP from paclitaxel was 74.1% in adsorbent treatment process under optimal conditions.