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http://dx.doi.org/10.9713/kcer.2016.54.1.89

Separation Behavior of Paclitaxel and Its Semi-synthetic Precursor 10-Deacetylpaclitaxel from Plant Cell Cultures  

Lee, Chung-Gi (Department of Chemical Engineering, Kongju National University)
Kim, Jin-Hyun (Department of Chemical Engineering, Kongju National University)
Publication Information
Korean Chemical Engineering Research / v.54, no.1, 2016 , pp. 89-93 More about this Journal
Abstract
In this study, we investigated the separation behavior of the anticancer agent paclitaxel and its semi-synthetic precursor 10-deacetylpaclitaxel (10-DAP) from plant cell cultures. As a result of sequential separation/purification performed by biomass extraction with solvent, liquid-liquid extraction, adsorbent treatment, hexane precipitation, and fractional precipitation, the adsorbent treatment was found to be the most effective in separating and recovering 10-DAP from paclitaxel. The optimal adsorbent type, crude extract/adsorbent ratio, and adsorbent treatment temperature were sylopute, 1:1.5 (w/w), and $20^{\circ}C$, respectively. The separation/recovery of 10-DAP from paclitaxel was 74.1% in adsorbent treatment process under optimal conditions.
Keywords
Paclitaxel; 10-Deacetylpaclitaxel; Separation; Adsorbent Treatment; Optimization;
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Times Cited By KSCI : 4  (Citation Analysis)
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