• Journal of Applied Biological Chemistry
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• 제55권2호
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• pp.71-77
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• 2012

Many of biochemical or physiological processes can be regulated by non-coding RNAs as well as coding RNAs in plants, animals and microbes. Recently, many small RNAs including microRNAs (miRNAs) and endogenous small interference RNAs (siRNAs) and long non-coding RNAs have been discovered from ubiquitous organisms including plants. Biotic and abiotic stresses are main causal agents of crop losses all over the world. Much efforts have been performed for understanding the complex mechanism of stress responses. Up to date, many of these researches have been related with the identification and investigation of stress-related proteins, showing limitation to resolve the complex mechanism. Recently, non-coding RNAs as well as coding genes have been gradually interested because of its potential roles in plant stress responses as well as other biophysical aspects. In this review, various potential roles of non-coding RNAs, especially miRNAs and siRNAs, are reviewed in relation with plant biotic and abiotic stresses.

• Asian Pacific Journal of Cancer Prevention
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• 제13권6호
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• pp.2813-2818
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• 2012

Background: Considerable evidence suggests that metadherin (MTDH) is a potentially crucial mediator of tumor malignancy and an important therapeutic target for simultaneously enhancing chemotherapy efficacy and reducing metastasis risk. Inhibition of MTDH expression by RNA interference has been shown in several previous research, but silencing MTDH expression by microRNA (miRNA) interference in breast cancer has not been established. In the present study, we investigated the role of MTDH-miRNA in down-regulation of proliferation, motility and migration of breast carcinoma cells. Methods: Expression vectors of recombinant plasmids expressing artificial MTDH miRNA were constructed and transfected to knockdown MTDH expression in MDA-MB-231 breast cancer cells. Expression of MTDH mRNA and protein was detected by RT-PCR and Western blot, respectively. MTT assays were conducted to determine proliferation, and wound healing assays and transwell migration experiments for cell motility and migration. Results: Transfection of recombinant a plasmid of pcDNA-MTDH-miR-4 significantly suppressed the MTDH mRNA and protein levels more than 69% in MDA-MB-231 breast cancer cells. This knockdown significantly inhibited proliferation, motility and migration as compared with controls. Conclusions: MTDH-miRNA may play an important role in down-regulating proliferation, motility and migration in breast cancer cells, and should be considered as a potential small molecule inhibitor therapeutic targeting strategy for the future.

• Corrosion Science and Technology
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• 제19권4호
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• pp.211-223
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• 2020

The external corrosion of buried piping can be controlled using both coating and cathodic protection. Several factors are involved in the damage and deterioration of the coating on pipes. There are many detection methods for coating defects on pipes and the direct current voltage gradient (DCVG) method is one of the most powerful methods. However, the detection reliability of DCVG can be affected by interferences such as stray current, metal objects connected to rectifiers, and copper grids. Therefore, this study focused on the interference effects of rectifiers and a copper grid on the reliability of coating flaw detection. As the length of the interference pipe connected to the rectifier increased, the reliability decreased. In contrast, as the distance between the pipe and the copper grid increased, the reliability of the coating flaw detection increased. The detection results produced by the DCVG method were discussed using current and potential simulations for a pipe with a rectifier and copper grid interference in the soil.

• 한국해양공학회지
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• 제37권6호
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• pp.247-255
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• 2023

Interference and collisions often occur in the loading process at shipyards. Existing simulation methods focus primarily on resource processes and schedules, and there is a lack of real-time reflection in the complex and highly variable loading process. This study aims to develop a spatial environment incorporating real-time product data, such as hulls, and confirms its effectiveness by simulating various construction scenarios. As a method, a near real-time spatial environment based on broadband laser scanning was established, with the situation of loading heavy cargo assumed when converting an existing ship into an LNG dual-fuel propulsion ship. A case study simulation of near-real-time cargo loading processes was then conducted using Unity 3D to confirm the interference and collision risks within the spatial environment. The results indicated that interference occurred in structures previously not identified in the design data, and a collision occurred during the loading object erection phase. The simulation confirmed that the identification of interference and collision risks during the erection phase highlights the need for a relocation or removal process of potential hazards before erection takes place. An improved erection simulation that integrates near real-time data could effectively prevent interference and collision risks.

• Corrosion Science and Technology
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• 제16권5호
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• pp.235-240
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• 2017

Regional cathodic protection has significant impact on pipeline integrity management. After risk analyses of a newly built gas distribution station constructed in an area with large dwelling density, risk score was high because of potential threat caused by galvanic corrosion. Except reinforced steel in concrete, there are four kinds of metal buried under earth: carbon steel, galvanized flat steel, zinc rod and graphite module. To protect buried pipeline from external corrosion, design and construction of regional cathodic protection was proposed. Current density was measured with potential using potential dynamic test and boundary element method (BEM) was used to calculate current requirement and optimize best anode placement during design. From our calculation on the potential, optimized conditions for this area were that an applied current was 3A and anode was placed at 40 meters deep from the soil surface. It results in potential range between $-1.128V_{CSE}$ and $-0.863V_{CSE}$, meeting the $-0.85V_{CSE}$ criterion and the $-1.2V_{CSE}$ criterion that no potential was more negative than $-1.2V_{CSE}$ to cause hydrogen evolution at defects in coating of the pipeline.

• Asian Pacific Journal of Cancer Prevention
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• 제15권22호
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• pp.9835-9839
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• 2014

Fisetin is an effective compound extracted from lacquer which has been used in the treatment of various diseases. Preliminary data indicate that it also exerts specific anti-cancer effects. However, the manner in which fisetin regulates cancer growth remains unknown. In this study, we elucidated interference of fisetin with targets of the nuclear factor ${\kappa}B$ signal transduction pathway activated by Epstein-Barr virus encoding latent membrane protein 1 (LMP1)in nasopharyngeal carcinoma (NPC) cells, Results showed that fisetin inhibited the survival rate of CNE-LMP1 cells and NF-${\kappa}B$ activation caused by LMP1. Fisetin also suppressed nuclear translocation of NF-${\kappa}B$ (p65) and $I{\kappa}B{\alpha}$ phosphorylation, while inhibiting CyclinD1, all key targets of the NF-${\kappa}B$ signal transduction pathway. It was suggested that interference effects of fisetin with signal transduction activated by LMP1 encoded by the Epstein-Barr virus may play an important role in its anticancer potential.

• 한국인터넷방송통신학회논문지
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• 제10권2호
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• pp.19-24
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• 2010

본 논문은 방송통신위원회의 정책에 따라 DTV 전환시 예상할 수 있는 DTV 서비스가 인접채널에 할당될 무선 마이크에 간섭을 주는 영향을 분석하였다. 분석을 위해 DTV 서비스가 채널 51(692 MHz ~ 698 MHz)에 할당되고 무선 마이크가 채널 52(698 MHz ~ 704 MHz)에 할당되는 시나리오를 가정하였다. Minimum Coupling Loss(MCL)를 이용하여 보호 이격거리를 산출하고 몬테 칼로(Monte Carlo) 분석을 이용하여 간섭 확률 0 %를 위한 보호 대역을 산출하였다. 그 결과 실내의 경우 1.71 km의 보호 이격거리와 2.4 MHz의 보호 대역이 산출되었고 실외의 경우 18.86 km의 보호 이격거리와 5.7 MHz의 보호 대역이 산출되었다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제8권7호
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• pp.2231-2245
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• 2014

Cognitive Radio (CR) has very promising potential to improve spectrum utilization by allowing unlicensed Secondary Users (SUs) to access the spectrum dynamically without disturbing licensed Primary Users (PUs). Mitigating interference is a fundamental problem in CR scenarios. This is particularly problematic for deploying CR in cellular networks, when users are located at the cell edge, as the inter-cell interference mitigation and frequency reuse are critical requirements for both PUs and SUs. Further cellular networks require higher cell edge performance, then SUs will meet more challenges than PUs. To solve the performance decrease for SUs at the cell edge, a novel Dynamic Spectrum Allocation (DSA) scheme based on Game Theory is proposed in this paper. Full frequency reuse can be realized as well as inter-cell interference mitigated according to SUs' sensing, measurement and interaction in this scheme. A joint power/channel allocation algorithm is proposed to improve both cell-edge user experience and network performance through distributed pricing calculation and exchange based on game theory. Analytical proof is presented and simulation results show that the proposed scheme achieves high efficiency of spectrum usage and improvement of cell edge SUs' performance.

• 한국통신학회논문지
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• 제36권3A호
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• pp.278-286
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• 2011

지향성 안테나는 네트워크의 공간 재사용 기술이 발전함에 따라 Ad Hoc 네트워크에서 널리 사용되고 있다. 그러나 확장된 전송영역은 이웃하는 수신 노드 간에 충돌을 일으키고, 이것은 지향성 안테나의 전송능력을 충분히 사용하지 못하게 하는 원인이 된다. 본 논문에서는 지향성 안테나를 위한 새로운 전력제어 MAC프로토콜을 제안한다. 이 프로토콜을 지원하기 위하여 이웃하는 수신 노드들 중에서 간섭 노드의 집합을 찾아내기 위한 기법을 개발한다. 그리고 전력 제어 알고리즘을 이용하여 최소 전송 전력을 계산한 후, 데이터를 전송한다. 이 프로토콜의 시뮬레이션 결과, 네트워크 성능이 향상되었으며 전력 소비가 감소하였음을 알 수 있었다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제13권6호
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• pp.3000-3022
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• 2019

Full-duplex (FD) technologies enable wireless nodes to simultaneously transmit and receive signal using the same frequency-band. The FD modes could improve their physical layer throughputs. However, in the wireless ad hoc networks, the FD communications also produce new interference risks. On the one hand, the interference ranges (IRs) of the nodes are enlarged when they work in the FD mode. On the other hand, for each FD pair, the FD communication may cause the potential hidden terminal problems to appear around the both sides. In this paper, to avoid the interference risks, we first model the IR of each node when it works in the FD mode, and then analyze the conditions to be satisfied among the transmission ranges (TRs), carrier-sensing ranges (CSRs), and IRs of the FD pair. Furthermore, in the media access control (MAC) layer, we propose a specific method and protocol for collision avoidance. Based on the modified Omnet++ simulator, we conduct the simulations to validate and evaluate the proposed FD MAC protocol, showing that it can reduce the collisions effectively. When the hidden terminal problem is serious, compared with the existing typical FD MAC protocol, our protocol can increase the system throughput by 80%~90%.