• 한국약용작물학회지
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• 제17권6호
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• pp.421-426
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• 2009

The purpose of this study was to identify soil physical characteristics as guideline for high yield potential in ginseng cultivated field which produced 6 years root. Harvest yields of ginseng to be divided by parent rock was in order of phyllite and red shale 3.1 kg/$3.3\;m^2$ > granite and gneiss schist 3.0 kg > basalt 2.6 kg > porphyry 2.2 kg in upland and forest soil. Also, with classified by topography, it was in order of foot slope and alluvial fan 3.2 kg/$3.3\;m^2$ > valley 3.0 kg > low hill 2.9 kg > hill, lave flow and dilluvial terrace 2.8 kg in survey tilth. Class determination of soil texture, it was in order of sandy loam 3.1 kg/$3.3\;m^2$ > loam and silt loam 3.0 kg > clay loam 2.9 kg > silt clay loam 2.8 kg in survey tilth. Slope condition of farming land, in case of sloping (2~7%), it was 3.1 kg/$3.3\;m^2$ but deep sloping (15~30%) caused decreasing harvest yield. In drainage classes (excessively, well and moderately well), there was no significantly different in harvest yields. Relationship between harvest yield and soil series, Production sites as yielding 3.0 kg/$3.3\;m^2$ were seven sites, also it was contained 14 soil series. Production sites as yielding 2.5~3.0 kg/$3.3\;m^2$ were eleven sites, it was contained 16 soil series. Production sites as yielding 2.0~2.5 kg/$3.3\;m^2$ were 10 sites, it was contained 4 soil series.

• Journal of Ginseng Research
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• 제47권5호
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• pp.662-671
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• 2023

Background: 20(S)-protopanaxadiol (PPD), a ginsenoside metabolite, has prominent benefits for the central nervous system, especially in improving learning and memory. However, its transcriptional targets in brain tissue remain unknown. Methods: In this study, we first used mass spectrometry-based drug affinity responsive target stability (DARTS) to identify the potential proteins of ginsenosides and intersected them with the transcription factor library. Second, the transcription factor PURA was confirmed as a target of PPD by biolayer interferometry (BLI) and molecular docking. Next, the effect of PPD on the transcriptional levels of target genes of PURA in brain tissues was determined by qRT-PCR. Finally, bioinformatics analysis was used to analyze the potential biological features of these target proteins. Results: The results showed three overlapping transcription factors between the proteomics of DARTS and transcription factor library. BLI analysis further showed that PPD had a higher direct interaction with PURA than parent ginsenosides. Subsequently, BLI kinetic analysis, molecular docking, and mutations in key amino acids of PURA indicated that PPD specifically bound to PURA. The results of qRT-PCR showed that PPD could increase the transcription levels of PURA target genes in brain. Finally, bioinformatics analysis showed that these target proteins were involved in learning and memory function. Conclusion: The above-mentioned findings indicate that PURA is a transcription target of PPD in brain, and PPD upregulate the transcription levels of target genes related to cognitive dysfunction by binding PURA, which could provide a chemical and biological basis for the study of treating cognitive impairment by targeting PURA.

• 한국육종학회지
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• 제41권3호
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• pp.288-291
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• 2009

"동해진미"는 중만생종이면서 쌀 품위가 좋고 밥맛이 양호하며 내병성이 양호한 품종으로 주요특성과 수량성을 요약하면 다음과 같다 1. 출수기는 평야지의 보통기 재배에서 8월 14일로 "화성벼"보다 2일 늦은 중만생종이다. 2. 주당수수와 수당립수는 "화성벼"보다 약간 많은 편이고 현미천립중은 22.4 g으로 "화성벼"보다 다소 가벼운 중립종이다. 3. 불시출수와 수발아가 안되는 편이고, 위조현상에 강하고 성숙기 엽노화가 느린 편이며 내냉성은 "화성벼"와 출수 지연일수는 비슷하나 임실률은 낮은 편이다. 4. 잎도열병은 중도이상의 저항성이며 도열병 내구저항성도 "화성벼"보다 강하다. 흰잎마름병($K_1{\sim}K_3$)과 줄무늬잎마름병에는 저항성이이나 오갈병 및 검은줄오갈병과 충해에는 약하다. 5. 심복백은 거의 없고 "화성벼"수준으로 맑고 투명하며 도정률, 완전미율이 높다. 아밀로스함량은 "화성벼"보다 조금 높고 단백질함량은 낮으며 밥맛은 양호하다. 6. "동해진미"의 쌀수량은 보통기 보비재배 6개소에서 평균 6.05 MT/ha로 "화성"보다 9% 증수되었으나, 이모작재배에서는 4.97 MT/ha, 만식재배에서는 5.13 MT/ha의 수량을 보였다.

• 한국육종학회지
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• 제50권4호
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• pp.424-433
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• 2018

수박(Citrullus lanatus)은 전세계에서 경제적으로 중요한 채소작물이며, 라이코펜과 시트룰린과 같은 기능성 물질을 함유하고 있다. Didymella bryoniae 병원균에 의해 발생하는 덩굴마름병은 수박 재배에서 가장 피해를 많이 주는 병해 중에 하나이다. 단일염기다형성(SNP)은 한 개 염기에 관해 개체 간에 발생하는 유전적 변이로서 유전자 연관 지도를 작성하는 것과 원예적 형질 및 병저항성에 연관된 분자표지를 개발하는 데 자주 사용된다. 본 연구에서는 수박에서 모친과 부친의 차세대 염기서열 재분석(next generation resequencing)을 통해 SNP 분자표지를 선발하였다. 식물재료는 C. lanatus '920533'(모친, 감수성)과 C. amarus 'PI 189225'(부친, 저항성) 및 이들의 $F_1$, $F_2$ 개체를 이용하였다. NGS 분석 결과, '920533'과 'PI 189225'에서 각각 13.6 Gbp와 13.1 Gbp의 염기서열을 얻었다. '920533'과 'PI 189225' 간의 SNP 수는 609만 개였고, 그 중 HRM 프라이머로 디자인이 가능한 SNP의 수는 354,860개였다. 그 중에 HRM 분석을 위한 330개 프라이머 쌍이 디자인되었다. 결과적으로 HRM 분석을 통해 총 61개의 HRM 분자표지를 개발하였다. 이번 연구의 결과는 SNP 기반의 유전자지도 작성에 이용될 수 있으며 덩굴마름병 저항성과 연관된 양적 형질 유전자좌(QTL) 분석에 활용될 수 있을 것이다.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제17권2호
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• pp.124-130
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• 2006

Objectives : The objective of this study was to evaluate the differences in patterns of temperament and character, as assessed by the Junior Temperament and Character Inventory (JTCI), between the children with attention-deficit/hyperactivity disorder (ADHD) and the control group in a community sample. In addition, we examined the potential differences on JTCI profiles according to the ADHD subtypes. Methods : Parents of 185 ADHD subjects (mean age $9.0{\pm}1.7$ years), as diagnosed by the DISC-IV, and 185 age- and gender-matched comparison children have completed the parent's version of the JTCI. Results : The ADHD group scored significantly higher in Novelty Seeking and lower in Persistence than the comparison group on JTCI. However, there were no significant differences in the temperament or character profiles by the ADHD subtypes. Conclusion : The results of this study suggest that the temperamental factors of higher Novelty Seeking and lower Persistence are related to ADHD. The temperament or character profiles in this study do not provide support for the distinctiveness of the ADHD subtypes.

• Journal of Ginseng Research
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• 제44권3호
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• pp.461-474
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• 2020

Background: Ginseng effectively reduces fatigue in both animal models and clinical trials. However, the mechanism of action is not completely understood, and its molecular targets remain largely unknown. Methods: By screening for proteins that interact with the primary components of ginseng (ginsenosides) in an affinity chromatography assay, we have identified muscle-type creatine kinase (CK-MM) as a potential target in skeletal muscle tissues. Results: Biolayer interferometry analysis showed that ginsenoside metabolites, instead of parent ginsenosides, had direct interaction with recombinant human CK-MM. Subsequently, 20(S)-protopanaxadiol (PPD), which is a ginsenoside metabolite and displayed the strongest interaction with CK-MM in the study, was selected as a representative to confirm direct binding and its biological importance. Biolayer interferometry kinetics analysis and isothermal titration calorimetry assay demonstrated that PPD specifically bound to human CK-MM. Moreover, the mutation of key amino acids predicted by molecular docking decreased the affinity between PPD and CK-MM. The direct binding activated CK-MM activity in vitro and in vivo, which increased the levels of tissue phosphocreatine and strengthened the function of the creatine kinase/phosphocreatine system in skeletal muscle, thus buffering cellular ATP, delaying exercise-induced lactate accumulation, and improving exercise performance in mice. Conclusion: Our results suggest a cellular target and an initiating molecular event by which ginseng reduces fatigue. All these findings indicate PPD as a small molecular activator of CK-MM, which can help in further developing better CK-MM activators based on the dammarane-type triterpenoid structure.

• Journal of Ginseng Research
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• 제39권3호
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• pp.230-237
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• 2015

Background: Colorectal cancer (CRC) is a leading cause of death worldwide. Chronic gut inflammation is recognized as a risk factor for tumor development, including CRC. American ginseng is a very commonly used ginseng species in the West. Methods: A genetically engineered $Apc^{Min/+}$ mouse model was used in this study. We analyzed the saponin composition of American ginseng used in this project, and evaluated its effects on the progression of high-fat-diet-enhanced CRC carcinogenesis. Results: After oral ginseng administration (10-20 mg/kg/d for up to 32 wk), experimental data showed that, compared with the untreated mice, ginseng very significantly reduced tumor initiation and progression in both the small intestine (including the proximal end, middle end, and distal end) and the colon (all p < 0.01). This tumor number reduction was more obvious in those mice treated with a low dose of ginseng. The tumor multiplicity data were supported by body weight changes and gut tissue histology examinations. In addition, quantitative real-time polymerase chain reaction analysis showed that compared with the untreated group, ginseng very significantly reduced the gene expression of inflammatory cytokines, including interleukin-$1{\alpha}$ (IL-$1{\alpha}$), IL-$1{\beta}$, IL-6, tumor necrosis factor-${\alpha}$, granulocyte-colony stimulating factor, and granulocyte-macrophage colony-stimulating factor in both the small intestine and the colon (all p < 0.01). Conclusion: Further studies are needed to link our observed effects to the actions of the gut microbiome in converting the parent ginsenosides to bioactive ginseng metabolites. Our data suggest that American ginseng may have potential value in CRC chemoprevention.

• 영재교육연구
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• 제25권2호
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• pp.217-236
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• 2015

이 연구는 사회적으로 성공한 인사들의 성취에 영향을 준 요인을 조사하고 영재교육의 개선방향을 도출하기 위해 수행되었다. 이를 위해 성취자 60명을 대상으로 6개 분야(교육, 전공, 가정 및 부모, 대인관계, 여가시간, 업적 및 성공요인)에 대해 반 구조화된 심층면담을 실시하였다. 연구 결과 많은 성취자들은 적극적인 학생이었으며, 가정은 화목하고 자율적이었고, 차별화 교육 및 학생 중심의 교육이 중요하다고 생각하였다. 개인적으로는 멘티를 지도하고 있지 않다는 응답자가 많았다. 전공 결정 및 교육은 재능과 흥미의 발견 시기에 맞춰 이뤄져야 한다고 응답하였다. 여가시간 활동으로는 운동, 음악, 독서를 중요시하였으며, 성취의 원동력이 된 것은 호기심, 자신감 등 개인 내적 특성이었다. 영재교육을 수혜한 성취자들은 영재교육의 경험이 성취에 중요한 영향을 준 것으로 인식하고 있었다. 면담 결과를 영재교육의 개선 방향과 관련지어 시사점을 논의하였다.

• 한국토양비료학회지
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• 제20권1호
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• pp.1-6
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• 1987

답토양(畓土壤) 6개유형중(個類型中) 가장 넓은 분포면적(分布面積)을 가진 보통답(普通畓)의 특성(特性)과 토지이용(土地利用) 및 토양생성(土壤生成)에 대(對)하여 조사연구(調査硏究)한 결과(結果)는 다음과 같다. 1. 보통답(普通畓)의 분포면적(分布面積)은 전체답면적(全體畓面積)의 33%에 해당(該當)되며 그중(中) 곡간(谷間) 및 선상지(扇狀地)에 54% 그 외(外)는 하해혼성평탄지(河海混成平坦地)와 하성평단지(河成平坦地)에 분포(分布)한다. 2. 보통답(普通畓)의 토양배수(土壤排水)는 대부분(大部分) 약간(若干) 불량(不良)으로 글라이화(化)된 식질(埴質) 및 식양질계(埴壤質系)로 물리성(物理性)이 양호(良好)한 편이고 토심(土深) 및 지하수위(地下水位)가 깊우며 토양(土壤)의 잠재생산능력은 높은 편이다. 3. 전체보통답(全體普通畓)의 이화학적특성(理化學的特性)을 보면 점토함량(粘土含量)은 표토(表土)와 심토(心土)가 많은 편(便)이며 토양반응(土壤反應)은 약(弱)한 산성(酸性)이었다. 유기물함량(有機物含量)은 표토(表土)는 있는 편(便)이나 심토(心土)는 적었다. 염기치환용량(鹽基置換容量)은 높고 치환성염기(置換性鹽基)는 표토(表土) 6.62, 심토(心土) 7.74 me/100 g 정도(程度)였다. 염기포화도(鹽基飽和度)는 높았으며 유효인산(有效燐酸)은 우리나라 답토양평균치(畓土壤平均値)인 60 ppm 보다는 다소(多少) 하회(下廻)하였다. 4. 생성원인(生成原因)은 제사기신층(第四期新層)에 속(屬)하지만 현세충적층인 것으로 보여지며 미농무성(美農務省)의 구분류체계(舊分類體系)에 의(依)하면 대부분(大部分) Low humic gley soils에 해당(該當)되고 F. A. O분류안(分類案)에 의(依)하면 Gleysols에 속(屬)하며 일본(日本)의 시비개선사업(施肥改善事業)에서는 토양군(土壤群)은 회색토양(灰色土壤), 토양아군(土壤亞群)은 회갈색토양(灰褐色土壤)이다. US soil taxonomy(Subgroup)에 의(依)하면 Typic 및 Fluventic Haplaquepts에 속(屬)한다.

• Journal of Preventive Medicine and Public Health
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• 제39권3호
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• pp.235-242
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• 2006

Objectives: We wanted to determine the distribution of the clustering of the metabolic risk factors and we wanted to evaluate the related factors in young schoolchildren. Methods: A cross-sectional study of metabolic syndrome was conducted in an elementary school in Seoul, Korea. We evaluated fasting glucose, triglyceride, HDL cholesterol, blood pressures and the body mass index, and we used parent-reported questionnaires to assess the potential risk factors in 261 children (136 boys, 125 girls). We defined the metabolic risk factors as obesity or at risk for obesity ($\geqq$ 85th percentile for age and gender), a systolic or diastolic blood pressure at $\geqq90th$ percentile for age and gender, fasting glucose at $\geqq110mg/dl$, triglyceride at $\geqq110mg/dl$ and HDL cholesterol at $\leqq40mg/dl$. Results: There were 15.7% of the subjects who showed clustering of two or more metabolic risk factors, 2.3% of the subjects who showed clustering for three or more risk factors, and 0.8% of the subjects who showed clustering for four or more risk factors. A multivariate analysis revealed that a father smoking more than 20 cigarettes per day, a mother with a body mass index of = $25kg/m^2$, and the child eating precooked or frozen food more than once per day were associated with clustering of two or more components, with the odds ratios of 3.61 (95% CI=1.24-10.48), 5.50 (95% CI=1.39-21.73) and 8.04 (95% CI=1.67-38.81), respectively. Conclusions: This study shows that clustering of the metabolic risk factors is present in young schoolchildren in Korea, with the clustering being associated with parental smoking and obesity as well as the child's eating behavior. These results suggest that evaluation of metabolic risk factors and intervention for lifestyle factors may be needed in both young Korean children and their parents.