• Title/Summary/Keyword: Potassium phosphate

Search Result 480, Processing Time 0.026 seconds

Control of Enzymatic Degradability of Microbial Polyester by Surface Modification (표면 개질을 통한 미생물합성 폴리에스테르의 효소분해속도 조절)

  • 이원기
    • Journal of Environmental Science International
    • /
    • v.11 no.12
    • /
    • pp.1315-1320
    • /
    • 2002
  • Since the enzymatic degradation of microbial poly[(R)-3-hydroxybutyrate-co-3-hydroxyvalerate] (P(3HB-co-3HV)) initially occurs by a surface erosion process, a degradation behavior could be controlled by the change of surface property. In order to control the rate of enzymatic degradation, plasma gas discharge and blending techniques were used to modify the surface of microbial P(3HB-co-3HV). The surface hydrophobic property of P(3HB-co-3HV) film was introduced by CF$_3$H plasma exposure. Also, the addition of small amount of polystyrene as a non-degradable polymer with lower surface energy to P(3HB-co-3HV) has been studied. The enzymatic degradation was carried out at 37 $^{\circ}C$ in 0.1 M potassium phosphate buffer (pH 7.4) in the presence of an extracellular PHB depolymerase purified from Alcaligenes facalis T1. Both results showed the significant retardation of enzymatic erosion due to the hydrophobicity and the enzyme inactivity of the fluorinated- and PS-enriched surface layers.

Optimization of the Sucrose and Ion Concentrations for Saikosaponin Production in Hairy Root Culture of Bupleurum falcatum

  • Ahn, Jun-Cheul;Chong, Won-Seog;Kim, Young-Soon;Hwang, Baik
    • Biotechnology and Bioprocess Engineering:BBE
    • /
    • v.11 no.2
    • /
    • pp.121-126
    • /
    • 2006
  • Saikosaponin productivity was examined in a Bupleurum falcatum L. BFHR2 hairy root culture in response to changes in the sucrose content $(2{\sim}8%)$, nitrogen content $(0{\sim}250mM\;NH_4NO_3)$, phosphate content $(0{\sim}12mM\;NaH_2PO_4)$, and the potassium content $(0{\sim}87.2 mM\; KCl)$ of the culture media. We found that the conditions for maximal saikosaponin production differed from those for optimal root growth. Highest saikosaponin yield was achieved for 8% sucrose, 62mM $NH_4NO_3$ 1.2 mM $NaH_2PO_4$, and 0.5mM KCl.

A Study on the Effects of N ,$P_2O_5$ and $K_2O$ Application on the Vegetation and Dry Matter Yield in the Mixed Sward of Ladino Clover and Crabgrass (Digitaria sanguinalis [L.]Scop.) (Ladino Clover와 바랭이 (Digitaria sanguinalis [ L.] Scop. ) 혼생초지에 있어서 질소, 인산 및 가리시용이 식생 및 건물수량에 미치는 영향에 관한 연구)

  • 김창주;성경일;김병완
    • Journal of The Korean Society of Grassland and Forage Science
    • /
    • v.16 no.2
    • /
    • pp.139-146
    • /
    • 1996
  • An experiment was canied out to investigate how to effect the application of nitrogen, phosphate and potassium on the vegetation and dry matter yield in the mixed sward of Ladino clover and crabgrass(Digitaria sanguinalis [L.] Scop.). The experimental sward was consisted of eight plots of non-application, K, P, PK, N, NK, NP and NPK. These plots were sown with seeds mixture of Ladino clover 5.0kg/ha and crabgrass 17.5kg/ha on 17 April 1976 in the forage experimental field of Kangwon National University, Chunchon, Korea, and were cut three times from 6 July to 4 October 1976.

  • PDF

Development of a Flow Injection Analysis Technique for On-line Monitoring of Xylitol Concentrations (자일리톨 농도의 온라인 모니터링을 위한 흐름주입분석기술 개발)

  • 이종일
    • KSBB Journal
    • /
    • v.17 no.4
    • /
    • pp.339-344
    • /
    • 2002
  • Flow injection analysis technique for monitoring of xylitol concentrations in biological processes has been developed using xylitol oxidase (XYO) immobilized on VA-Epoxy Biosynth carrier. The immobilized XYO cartridge has been integrated into a FIA system with an oxygen electrode and systematically investigated with regards to the factors which can affect the activity of the immobilized XYO, such as pH, temperature, salt concentration etc. The activity of the immobilized XYO increased with the temperature ($19.0 - 29.0^{circ}C$) and sample injection volume ($75-250\muL$) and molarity of potassium phosphate buffer (0.1-1 M), but it reached the highest value at pH 8.5. The XYO-FIA system has been also applied for on-line monitoring of xylitol concentrations in a reactor and showed good operational stability and agreement with off-line data measured with HPLC.

Adhesion Strength Properties of Matrix Using Magnesia Accroding to Potassium Phosphate Addition Ratio (산화마그네슘을 활용한 경화체의 지연제 첨가율에 따른 접착강도 특성)

  • Kim, Tae-Hyun;Sin, Jin-Hyun;Lee, Sang-Soo
    • Proceedings of the Korean Institute of Building Construction Conference
    • /
    • 2016.05a
    • /
    • pp.34-35
    • /
    • 2016
  • Most of the construction adhesive may contain harmful component to the human body with an organic adhesive. In addition, when the adhesive caused hydroquinone, alkyl benzophenone, etc, also it raises the skin diseases to workers. Therefore, the inorganic adhesive development is necessary. This study was a study to know adhesive strength properties that adhesive using a magnesium oxide according to the addition ratio of the retarder. It has experiments to two types of magnesium oxide and type and ratio of retarder differently.

  • PDF

Anticorrosive Ability and Mechanism of Hydroxyapatite Pigment

  • Park, J.H.;Lee, G.D.;Nishikata, A.;Tsuru, T.
    • Corrosion Science and Technology
    • /
    • v.4 no.1
    • /
    • pp.15-18
    • /
    • 2005
  • Hydroxyapatite(HAp) was synthesized using the waste sludge from semiconductor process and used as an anticorrosive pigment. The water absorption of coating pigmented with anticorrosive pigment and the corrosion at interface between coating and substrate were monitored using AC impedance techniques. The anticorrosive performance of HAp was compared with those of red lead(RL) and zinc potassium chromate(ZPC), which have been known as representative anticorrosive pigments. The amount of absorbed water in ZPC- and HAp- pigmented coatings was much higher compared to that in RL-pigmented and unpigmented film. However, it seems that the water absorbed into HAp- or ZPC-pigmented film is beneficial to anticorrosive function. The anticorrosive performance of HAp is superior or at least comparable to those of ZPC and RL. The excellent anticorrosive properties of HAp can be explained by its passivating ability, caused by the reaction of the soluble component of HAp with Fe to form iron phosphate in the presence of water.

Effect of Macronutritional-element Deficiencies on the Growth and Photosynthetic Activity of Chlorella Cells (Chlorella의 다량원소 결핍에 따른 생장 및 광합성능의 변화)

  • 장래성;이영녹
    • Korean Journal of Microbiology
    • /
    • v.13 no.1
    • /
    • pp.31-36
    • /
    • 1975
  • Chlorella ellipsoidea cells were cultured in a phosphate, magnesium, sulfur or potassium-free medium. Some portions of cells were taken out at intervals during the culture, and physiological activities such as growth rate, reproduction, photosynthetic activity, and biosynthesis of cholorophyll of macro-element deficient cells were measured. 1) Generally, growth rate, reproduction, photosynthetic activities, and biosynthesis of chlorophyll of the macro-element deficient cells decreased more or less, compared with those of the normal cells. 2) The growth and reproduction of the algal cells in sulfur, or magnesium free medium were retarted severely with the chlorosis ; the photosynthetic activity and the content of chlorophyll showed the same tendency. 3) It is considered that the decrease in growth rate of macro-element deficient Chlorella cells is due to the decrease in photosynthetic activity owing to the decrease in chlorophyll content of the cells.

  • PDF

Effects of Fertilizer Levels on Dry Matter Yield and Nutritional Quality of Forage Rye

  • Kwon, Byung-Sun
    • Plant Resources
    • /
    • v.7 no.3
    • /
    • pp.181-186
    • /
    • 2004
  • To find out the optimum fertilizer level for high yielding variety, Paldanghomil, experiment was conducted with 15 compositions of fertilizer levels at the experiment field of forage rye in Sunchon National University from Sep. 1998 to Aug. 1999. The effects of nitrogen fertilizer on plant growth were large significant but increasing rate of application in potassium and phosphate fertilizers above 10kg/10a had negligible effects on plant growth. Raising nitrogen application rate of fertilizers turned out to be 18-10-10kg/l0a of N-P$_2$O$_{5}$-K$_2$O. Content of crude protein was the highest and that of crude fiber such as NDF, ADF, cellulose and lignin were lowest at this rate. Furthermore, IVDMD was high and dry matter yield were the highest at the optimum rate.e.

  • PDF

Laser Light Modulation with Resonated Crystal Modulator (공진형 결정체변조기에 의한 레이저광변조)

  • 양인응;김영권
    • Journal of the Korean Institute of Telematics and Electronics
    • /
    • v.7 no.2
    • /
    • pp.1-12
    • /
    • 1970
  • In this paper, theoretical and experimental study about the light modulation at X-band(9375MHz) micro wave, using the linear electro-optic effect, has been described. The electro-optic crystal, KDP (potassium dihydrogen phosphate)is used for modulator crystals which is composed resonated modulator. Output performances of modulated wave through movable short positions are presented. It was critically respond from 2. And the output modulated intensity was compared with theoretical curve. Consequently, in spite of many problems remain yet, most promissing and more sucessful modulator configurations are compleely given.

  • PDF

Fermentative Production of Cytidine 5'-diphosphate Choline by Candida utilis (Candida utilis에 의한 Cytidine 5'-diphosphate Choline의 발효생산)

  • 이인선;조정일;조규선
    • Journal of the East Asian Society of Dietary Life
    • /
    • v.5 no.3
    • /
    • pp.233-238
    • /
    • 1995
  • CDP-choline is known as an intermediate of lecithin biosynthesis, and as an important drug for nervous diseases of the brain, For the bioconversion of CMP and choline to CDP-choline, ATP is required as an energy source. In these studies, the biosynthetic reaction of CDP-choline was coupled with ATP regenerating system by glycolysis. As a microorganism containing the highest conversion activity of CMP and choline to CDP-choline, Candida utilis ATCC 42416 was selected. The optimum reaction condition were 50mM choline chloride, 20mM CMP, 100mM potassium phosphate (pH8.0), 300mM glucose, 50mM MgSO4, 10% dried cells with shaking incubation at 3$0^{\circ}C$. The reaction was thus performed for 10 hours under the above optimum conditions. The concentration of CDP-choline was 16mM(80% in conversion ratio).

  • PDF