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Development of a Flow Injection Analysis Technique for On-line Monitoring of Xylitol Concentrations  

이종일 (전남대학교 응용화학공학부, 생물공정기술연구실)
Publication Information
KSBB Journal / v.17, no.4, 2002 , pp. 339-344 More about this Journal
Abstract
Flow injection analysis technique for monitoring of xylitol concentrations in biological processes has been developed using xylitol oxidase (XYO) immobilized on VA-Epoxy Biosynth carrier. The immobilized XYO cartridge has been integrated into a FIA system with an oxygen electrode and systematically investigated with regards to the factors which can affect the activity of the immobilized XYO, such as pH, temperature, salt concentration etc. The activity of the immobilized XYO increased with the temperature ($19.0 - 29.0^{circ}C$) and sample injection volume ($75-250\muL$) and molarity of potassium phosphate buffer (0.1-1 M), but it reached the highest value at pH 8.5. The XYO-FIA system has been also applied for on-line monitoring of xylitol concentrations in a reactor and showed good operational stability and agreement with off-line data measured with HPLC.
Keywords
Flow injection analysis; xylitol oxidase; monitoring of xylitol;
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