• The Plant Pathology Journal
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• 제26권4호
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• pp.353-359
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• 2010

In order to control postharvest spoilage of satsuma mandarin fruits, rhizobacteria were isolated from soil samples. The Pantoea agglomerans strain 59-4 (Pa 59-4) which suppresses the decay of mandarin fruit by green and blue mold, was tested for the control efficacy and its mode of action was investigated. Pa 59-4 inhibited infection by green and blue mold on wounded mandarins, which were artificially inoculated with a spore suspension of Penicillium digitatum and P. italicum with control efficacies of 85-90% and 75-80%, respectively. The biocontrol efficacy was increased by raising the concentration of cells to between $10^8$ and $10^9\;cfu/ml$, and pretreatment with the antagonist prevented subsequent infection by green mold. The population of Pa 59-4 was increased more than 10 fold during the 24 hr incubation at $20^{\circ}C$, indicating that colonization of the wound site might prevent the infection by green mold. Despite poor antifungal activity, the Pa 59-4 isolate completely inhibited the germination and growth of P. digitatum spores at $1{\times}10^8\;cfu/ml$. We argue that the control efficacy was mediated by nutrient competition. Overall, the effective rhizobacterium, Pa 59-4, was shown to be a promising biocontrol agent for the postharvest spoilage of mandarin fruits by green and blue mold.

• 원예과학기술지
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• 제31권3호
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• pp.299-307
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• 2013

A radish (Raphanus sativus L.) polygalacturonase-inhibiting protein (PGIP) gene was cloned and compared to the PGIP gene (BrPGIP2) from Chinese cabbage (Brassica rapa ssp. pekinensis) in order to gain more information on controlling a disease and improving produce quality. To clone the radish PGIP gene, primers were designed based on conserved sequences of two PGIP genes (BnPGIP1 and BnPGIP2) from rape (B. napus L. ssp. oleifera), Chinese cabbage and Arabidopsis thaliana. PCR cloning was performed with cDNA from the stigma of radish 'Daejinyeoreum' as a template to confirm DNA fragments which were about 600 base pair in size. Sequence analysis revealed 84.1% homology with BrPGIP2 and 70.1% with BnPGIP1. DNA walking was conducted to confirm the open reading frame of 972 bp, and the gene was named RsPGIP1. RsPGIP1 consisting with 323 amino acids (aa) has a high leucine content (54/323) and contains 10 leucine-rich repeat domains, as do most BrPGIPs of Chinese cabbage. The gene expression of RsPGIP1 was induced by abiotic stresses and methyl jasmonate. It showed enrichment in the stigma and the primary root than a leaf. Cloning RsPGIP1 will aid to further apply practices on postharvest quality maintenance and disease control of the root.

• The Plant Pathology Journal
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• 제25권4호
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• pp.361-368
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• 2009

The efficacy of the combination of Burkholderia cepacia B23 with 0.75% chitosan and 3% calcium chloride ($CaCl_2$) as a biocontrol treatment of anthracnose disease of papaya caused by Colletotrichum gloeosporioides, was evaluated during storage. The growth of B. cepacia B23 in papaya wounds and on fruit surfaces was not affected in presence of chitosan and $CaCl_2$ or combination throughout the storage period. The combination of B. cepacia B23 with chitosan-$CaCl_2$ was more effective in controlling the disease than either B. cepacia B23 or chitosan or other combination treatments both in inoculated and naturally infected fruits. Combining B. cepacia B23 with chitosan-$CaCl_2$ gave the complete control of anthracnose infection in artificially inoculated fruits stored at $14^{\circ}C$ and 95% RH for 18 days, which was similar to that obtained with fungicide $benocide^{(R)}$. Moreover, this combination offered a greater control by reducing 99% disease severity in naturally infected fruits at the end of 14 days storage at $14^{\circ}C$ and 95% RH and six days post ripening at $28\pm2^{\circ}C$, which was superior to that found with $benocide^{(R)}$ or other treatments tested. Thus, postharvest application of B. cepacia B23 with chitosan-$CaCl_2$ as enhancers represents a promising alternative to synthetic fungicides for the control of anthracnose in papaya during storage.

• The Plant Pathology Journal
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• 제29권2호
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• pp.168-173
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• 2013

Apple ring rot disease, caused by Botryosphaeria dothidea (Moug. ex. Fr) Ces. et de Not., is one of the most important diseases on apple fruits. In this study, strain 9001 isolated from healthy apple fruits from an infested orchard was evaluated for its biocontrol activity against apple ring rot in vitro and in vivo. Strain 9001 showed obvious antagonistic activity to B. dothidea YL-1 when plated on potato dextrose agar. Soaking healthy apples in the bacterial suspensions of strain 9001 prior to artificial inoculation of fungal pathogen resulted in a dramatic decrease in disease incidence when compared to the control. Moreover, either field application in the growth season or postharvest treatment of apples from infected orchards with bacterial suspensions of strain 9001 resulted in significantly reduced disease incidence within the storage period for 4 months at room temperature. Based on the phylogenetic analysis of 16S rRNA and the gyrA gene, strain 9001 was identified as Bacillus amyloliquefaciens. These results indicated that B. amyloliquefaciens 9001 could be a promising agent in biocontrol of apple ring rot on fruit, which might help to minimize the yield loss of apple fruit during the long postharvest period.

• 농업과학연구
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• 제39권1호
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• pp.117-123
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• 2012

Pathogenic fungi and bacteria such as Pectobacterium atrosepticum, Clavibacter michiganensis subsp. sepedonicus, Verticillium albo-atrum, and Rhizoctonia solani were the major microorganism which causes diseases in seed potato during postharvest process. Current detection method for disease-infected seed potato relies on human inspection, which is subjective, inaccurate and labor-intensive method. In this study, a reflectance spectroscopy was used to classify sound and disease-infected seed potatoes with the spectral range from 400 to 1100 nm. Partial least square discriminant analysis (PLS-DA) with various preprocessing methods was used to investigate the feasibility of classification between sound and disease-infected seed potatoes. The classification accuracy was above 97 % for discriminating disease seed potatoes from sound ones. The results show that Vis/NIR reflectance method has good potential for non-destructive sorting for disease-infected seed potatoes.

• 한국식품저장유통학회:학술대회논문집
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• 한국식품저장유통학회 2007년도 학술발표회
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• pp.65-72
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• 2007

Phytochemicals have long been known to hold a number of physiological benefits, including antioxidant, anticardiovascular activities and anticancer. The profitable effects of phytochemicals from food sources such as vegetables and fruits, with respect to neurodegeneration, are only beginning to receive increased attention. Alzheimer's disease (AD) is one of the major neurodegenerative diseases for which no treatment is available, and characterized by loss of cognition and memory. Many recent studies show that the brain of AD patient is subjected to increased oxidative stress resulting from free radical damage, and the resulting cellular malfunctions are widely believed to be responsible for neuronal degeneration in AD. In this study, the relative relation between AD and phytochemicals were surveyed.

• 식물병연구
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• 제9권4호
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• pp.201-204
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• 2003

Botryosphaeria dothidea, Diaporthe actinidiae 및 Botrytis cinerea에 의해 발생하는 참다래 저장병을 예방하기 위하여 참다래 최적 후숙 조건을 조사하였다. B. dothidea와 D. actinidiae의 균사 생육적온은 각각 $26{\sim}35^{\circ}C$와 $26{\sim}29^{\circ}C$였으며, B. cinerea의 균사 생육적온은 $20{\sim}26^{\circ}C$였으며, 저장병 발병율은 균사 생육적온과 밀접한 관계가 있었다. 참다래 과실을 후숙시킬 경우 고온일수록 후숙 기간을 단축시킬 수 있으나 후숙과 함께 저장병 발생도 증가하였다. 참다래 과실을 $17^{\circ}C$의 후숙 온도에서 20일 동안 후숙시키는 것이 가장 이상적인 후숙 조건이었다.

• 식물병연구
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• 제9권4호
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• pp.205-208
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• 2003

2000년과 2001년 참다래 저장병 방제 실험 결과 터부코나졸 수화제, 이프로 수화제 및 후루실라졸 수화제의 살포적기는 7월 초였고 10일 간격 3회 이상 처리에서 방제 효과가 높았다. 세 가지 약제는 모두 6월 중순부터 10일 간격으로 4회 살포하는 것이 참다래 저장병에 대한 최대 방제효과를 얻을 수 있는 최적 살포 프로그램으로 2002년 포장실험 결과 확인되었다. 터부코나졸 수화제와 이프로 수화제 뿐만 아니라 후루실라졸 수화제는 우리나라에서 참다래 저장병 약제로 등록된 베노밀 수화제와 지오판 수화제를 대체할 수 있는 예방약제로 검증되었으므로 이 약제들을 활용하게 되면 약제의 다양화를 통하여 약제 저항성균의 출현을 예방할 수 있고 식품 안전과 환경보전을 위하여 약제 사용횟수도 줄일 수 있을 것으로 기대된다.

• 식물병연구
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• 제24권3호
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• pp.175-181
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• 2018

2013년과 2014년 자연감염된 과수원에서 재배되는 국산 골드키위 품종인 해금에서 발생하는 궤양병, 세균성점무늬병과 과실무름병의 발병률을 그린키위 품종인 헤이워드와 비교하였다. 2013년과 2014년 P. syringae pv. actinidiae biovar 2 (Psa2)에 자연감염된 키위 과수원에서 함께 재배되고 있는 헤이워드 품종에서궤양병발병률은각각18.5%와17.3%인반면에해금 품종에서 궤양병 발병률은 1.2%와 0%였다. 2013년과 2014년 A. valerianellae에 의한 세균성점무늬병 발병률은 63.5%와 16.2%였으나 해금 품종에서는 전혀 발병되지 않았다. 2013년 관행방식에 의해 함께 재배되고 있는 과수원에서 헤이워드와 해금 품종에서 과실무름병 평균 발병률은 각각 24.2%와 6.3%였으며 2014년에는 각각 20.5%와 4.4%였다. 두 품종 모두에서 과실무름병을 일으키는 가장 중요한 병원균은 B. dothidea로 확인되었다.

• 한국식품영양과학회지
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• 제23권2호
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• pp.315-321
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• 1994

In order to prevent the postharvest decay and to promote the freshness retention of fruits and vegetables grapefruit seed extract(GFSE), natural microorganism control agent, was applied to the preservation of fresh fruits and vegetables. Freshfruits and vegetables treated with GFSE and stored in polyethylene film (0.1mm) at 1$0^{\circ}C$-15$^{\circ}C$ of natural low temperature low kept better qualities in color and texture than the GFSE -not- treated control. The treatment using GFSE ina 250ppm to 500ppm concentration seemed to be an effective one for the control of Botrytis cinerea isolated in red wine grapes. After 4 weeks of storage the firmness rate of cucumbers treated with the dilute GFSE was four times higher than that of non-treated ones. GFSE showed effective inhibitory action towards plant pathological bacteria and fungi which were involved in the decay of fruits and vegetables. Minimum inhibitory concentrations of GFSE towards them were in the range of 250ppm to 500ppm .Direct visualization of microbial cells and spores using electron microscopy showed microbial cells and fungal spores the function of which was destroyed by treating with the dilute solutions of GFSE. It was observed that GFSE would reduced disease damages and have bactericide & fungicide properties during the storage of such fruits and vegetables as egg plant, wild edible greens , kumquat, and kiwi fruit.