The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Suppression of Green and Blue Mold in Postharvest Mandarin Fruit by Treatment of Pantoea agglomerans 59-4

  • Yu, Sang-Mi (Division of Biotechnology, Chonbuk National University) ;
  • Kim, Yong-Ki (Agricultural Science (NAAS), RDA) ;
  • Nam, Hyo-Song (Division of Biotechnology, Chonbuk National University) ;
  • Lee, Young-Kee (Crop Protection Division, Department of Agricultural Biology, NAAS, RDA) ;
  • Lee, Seung-Don (Crop Protection Division, Department of Agricultural Biology, NAAS, RDA) ;
  • Lee, Kui-Jae (Division of Biotechnology, Chonbuk National University) ;
  • Lee, Yong-Hoon (Division of Biotechnology, Chonbuk National University)
  • Received : 2010.08.18
  • Accepted : 2010.11.12
  • Published : 2010.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

In order to control postharvest spoilage of satsuma mandarin fruits, rhizobacteria were isolated from soil samples. The Pantoea agglomerans strain 59-4 (Pa 59-4) which suppresses the decay of mandarin fruit by green and blue mold, was tested for the control efficacy and its mode of action was investigated. Pa 59-4 inhibited infection by green and blue mold on wounded mandarins, which were artificially inoculated with a spore suspension of Penicillium digitatum and P. italicum with control efficacies of 85-90% and 75-80%, respectively. The biocontrol efficacy was increased by raising the concentration of cells to between $10^8$ and $10^9\;cfu/ml$, and pretreatment with the antagonist prevented subsequent infection by green mold. The population of Pa 59-4 was increased more than 10 fold during the 24 hr incubation at $20^{\circ}C$, indicating that colonization of the wound site might prevent the infection by green mold. Despite poor antifungal activity, the Pa 59-4 isolate completely inhibited the germination and growth of P. digitatum spores at $1{\times}10^8\;cfu/ml$. We argue that the control efficacy was mediated by nutrient competition. Overall, the effective rhizobacterium, Pa 59-4, was shown to be a promising biocontrol agent for the postharvest spoilage of mandarin fruits by green and blue mold.

Keywords

References

  1. Bonaterra, A., Mari, M., Casalini, L. and Montesinos, E. 2003. Biological control of Monilinia laxa and Rhizopus stolonifer in postharvest of stone fruit by Pantoea agglomerans EPS125 and putative mechanisms of antagonism. Int. J. Food Microbiol. 84:93-104. https://doi.org/10.1016/S0168-1605(02)00403-8
  2. Bryk, H., Dyki, B. and Sobiczewski, P. 1998. Antagonistic effect of Erwinia herbicola on in vitro spore germination and germ tube elongation of Botrytis cinerea and Penicillium expansum. Biocontrol 43:97-106. https://doi.org/10.1023/A:1009914612914
  3. Bull, C. T., Stack, J. P. and Smilanick, J. L. 1997. Pseudomonas syringae strains ESC-10 and ESC-11 survive in wounds on citrus and control green and blue molds of citrus. Biological Control 8:81-88. https://doi.org/10.1006/bcon.1996.0476
  4. Demoz, B. T. and Korsten, L. 2006. Bacillus subtilis attachment, colonization, and survival on avocado flowers and its mode of action on stem-end rot pathogens. Biological Control 37:68-74. https://doi.org/10.1016/j.biocontrol.2005.11.010
  5. Droby, S., Cohen, A., Weiss, B., Horev, B., Chalutz, E., Katz, H., Keren-Tzur, M. and Shachnai, A. 1998. Commercial testing of Aspire: a yeast preparation for the biological control of postharvest decay of citrus. Biological Control 12:97-101. https://doi.org/10.1006/bcon.1998.0615
  6. Droby, M., Wisniewski, D., Macarisin, D. and Wilson, C. 2009. Twenty years of postharvest biocontrol research: is it time for a new paradigm? Postharvest Biol. Technol. 52:137-145. https://doi.org/10.1016/j.postharvbio.2008.11.009
  7. Eckert, J. W. and Ogawa, J. M. 1988. The chemical control of postharvest diseases: deciduous fruits, berries, vegetables and root/tuber crops. Annu. Rev. Phytopathol. 26:433-469. https://doi.org/10.1146/annurev.py.26.090188.002245
  8. Frances, J. 2000. Experimental bases for implementation of a rational control of postharvest fungal rots and physiological disorders on pear in Girona cooperatives. PhD thesis, Universidad Pública de Navarra, Spain.
  9. Huang, Y., Daverall, B. J., Morris, S. C. and Wild, B. L. 1993. Biocontrol of postharvest orange diseases by a strain of Pseudomonas cepacia under semi-commercial conditions. Postharvest Biol. Technol. 3:293-304. https://doi.org/10.1016/0925-5214(93)90010-Z
  10. Janisiewicz, W. J., Tworkoski, T. J. and Sharer, C. 2000. Characterizing the mechanism of biological control of postharvest diseases on fruits with a simple method to study competition for nutrients. Phytopathology 90:1196-1200. https://doi.org/10.1094/PHYTO.2000.90.11.1196
  11. Kim, Y. K., Hong, S. J., Jee, H. J., Park, J. H., Han, E. J., Park, K. S., Lee, S. Y. and Lee, S. D. 2010a. Biological control of garlic blue mold using Pantoea agglomerans S59-4. The Kor. J. Pest. Sci. 14:148-156.
  12. Kim, Y. K., Hong, S. J., Jee, H. J., Park, J. H., Han, E. J., Park, K. S., Lee, S. Y. and Lee, S. D. 2010b. Elucidation of mode of action of Pantoea agglomerans 59-4 for controlling postharvest disease of garlic blue mold. Res. Plant Dis. 16:163-169. https://doi.org/10.5423/RPD.2010.16.2.163
  13. Meziane, H., Gavriel, S., Ismailov, Z., Chet, I., Chernin, L. and Höfte, M. 2006. Control of green and blue mould on orange fruit by Serratia plymuthica strains IC14 and IC1270 and putative modes of action. Postharvest Biol. Technol. 39:125-133. https://doi.org/10.1016/j.postharvbio.2005.10.007
  14. Mikani, A., Etebarian, H. R., Sholberg, P. L., O’Gorman, D. T., Stokes, S. and Alizadeh, A. 2008. Biological control of apple gray mold caused by Botrytis mali with Pseudomonas fluorescens strains. Postharvest Biol. Technol. 48:107-112. https://doi.org/10.1016/j.postharvbio.2007.09.020
  15. Nakkeeran, S., Dilantha Fernando, W. G. and Siddiqui, Z. A. 2005. Plant growth promoting rhizobacteria formulations and its scope in commercialization for the management of pests and diseases. in Z.A. Siddiqui (ed.). PGPR: Biocontrol and biofertilization, Springer. 257-296.
  16. Nunes, C., Usall, J., Teixido, N., Fons, E. and Vinas, I. 2001. Postharvest biological control by Pantoea agglomerans (CPA-2) on Golden delicious apples. J. Appl. Microbiol. 92:247-255. https://doi.org/10.1046/j.1365-2672.2002.01524.x
  17. Poppe, L., Vanhoutte, S. and Hofte, M. 2003. Modes of action of Pantoea agglomerans CPA-2, an antagonist of postharvest pathogens on fruits. Eur. J. Plant Pathol. 109:963-973. https://doi.org/10.1023/B:EJPP.0000003747.41051.9f
  18. Sharma, R. R., Sing, H. D. and Sing, H. R. 2009. Biological control of postharvest diseases of fruits and vegetables by microbial antagonists. Biological control 50:205-221. https://doi.org/10.1016/j.biocontrol.2009.05.001
  19. Singh, V. and Deverall, B. J. 1984. Bacillus subtilis as a control agent against fungal pathogens of citrus fruit. Trans. Bri. Mycol. Soc. 83:487-490. https://doi.org/10.1016/S0007-1536(84)80045-5
  20. Spadaro, D. and Gullino, M. L. 2004. State of the art and future prospects of the biological control of postharvest fruit diseases. Int. J. Food Microbiol. 91:185-194. https://doi.org/10.1016/S0168-1605(03)00380-5
  21. Teixido, N., Usall, J., Palou, L., Asensio, A., Nunes, C. and Vinas, I. 2001. Improving control of green and blue molds of oranges by combining Pantoea agglomerans (CPA-2) and sodium bicarbonate. Eur. J. Plant Pathol. 107:658-694.
  22. Torres, R., Nunes, C., Garcia, J. M., Abadias, M., Vinas, I., Manso, T., Olmo, M. and Usall, J. 2007. Application of Pantoea agglomerans CPA-2 in combination with heated sodium bicarbonate solutions to control the major postharvest diseases affecting citrus fruit at several Mediterranean locations. Eur. J. Plant Pathol. 118:73-83. https://doi.org/10.1007/s10658-007-9120-1
  23. Usall, J., Smilanicket, J., Palou, L., Denis-Arrue, N., Teixido, N., Torres, R. and Viñas, I. 2008. Preventive and curative activity of combined treatments of sodium carbonates and Pantoea agglomerans CPA-2 to control postharvest green mold of citrus fruit. Postharvest Biol. Technol. 50:1-7. https://doi.org/10.1016/j.postharvbio.2008.03.001

Cited by

  1. Isolation and Characterization of an Antagonistic Endophytic Bacterium Bacillus velezensis CB3 the Control of Citrus Green Mold Pathogen Penicillium digitatum vol.40, pp.2, 2012, https://doi.org/10.4489/KJM.2012.40.2.118
  2. Genes involved in nutrient competition byPseudomonas putidaJBC17 to suppress green mold in postharvest satsuma mandarin vol.55, pp.7, 2015, https://doi.org/10.1002/jobm.201400792
  3. Effect of light quality on Bacillus amyloliquefaciens JBC36 and its biocontrol efficacy vol.64, pp.3, 2013, https://doi.org/10.1016/j.biocontrol.2012.11.004
  4. Biocontrol of green and blue molds in postharvest satsuma mandarin usingBacillus amyloliquefaciensJBC36 vol.22, pp.10, 2012, https://doi.org/10.1080/09583157.2012.719150