• 한국어병학회지
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• 제34권2호
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• pp.149-159
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• 2021

Genus Megalocytivirus cause red sea bream iridoviral disease (RSIVD) and scale drop disease (SDD). Based on the phylogeny of the major capsid protein (MCP) and adenosine triphosphatase (ATPase) genes, megalocytiviruses except for SDD virus (SDDV) could be three different genotypes, red sea bream iridovirus (RSIV), infectious spleen and kidney necrosis (ISKNV), and turbot reddish body iridovirus (TRBIV). In this study, primary cells derived from the caudal fin of rock bream (Oplegnathus fasciatus) grew at 25℃ in Leibovitz's medium supplemented with 10% (v/v) fetal bovine serum and primocin (100 ㎍/mL). Rock bream fin (RBF) cells exhibited susceptibility to infections by different genotypes of megalocytiviruses (RSIV, ISKNV and TRBIV) with the appearance of cytopathic effects with an increase in the viral genome copy number. Furthermore, compared to grunt fin (GF) cells, even though 10 times lower number of RSIV genome copies were inoculated in RBF cells, viral genome copy number produced on RBF cells were 44 times higher than that of GF cells at 7 d post-inoculation. As the isolated RBF cells are sensitive to different genotypes of megalocytiviruses (RSIV, ISKNV and TRBIV), they can be used for future studies regarding in vitro viral infection and subsequent diagnosis.

• Journal of Animal Science and Technology
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• 제63권4호
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• pp.766-777
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• 2021

Bezafibrate, a fibrate drug used as a lipid-lowering agent to treat hyperlipidemia, is a pan-agonist of peroxisome proliferator-activated receptor alpha. It can enhance mitochondrial fatty acid oxidation, oxidative phosphorylation, and mitochondrial biogenesis. After ovulation, oocytes may get arrested at the metaphase II (MII) stage until fertilization beyond optimal timing, which is termed as post-ovulatory aging. Post-ovulatory aging is a disease that degrades DNA, mitochondria, and oxidative system, and has a negative impact on embryo development and quality; however, the impact of bezafibrate during post-ovulatory aging has not been fully defined. In the present study, we assessed the ability of bezafibrate to prevent the progression of aging in in vitro conditions as well as the underlying mechanisms in pigs. An appropriate concentration of this drug (50 µM) was added, and then oxidative stress, reactive oxygen species downstream, mitochondrial biogenesis, and mitochondrial function were analyzed via immunofluorescence staining and real-time polymerase chain reaction. Bezafibrate significantly alleviated reactive oxygen species and ameliorated glutathione production simultaneously in oocytes and embryos. Moreover, it diminished H2A.X and attenuated CASPASE 3 expression produced by oxidative stress in oocytes and embryos. Furthermore, bezafibrate remarkably improved the mitochondrial function and blastocyst quality as well as markedly reduced the mitochondria/TOM20 ratio and mtDNA copy number. The elevated PARKIN level indicated that mitophagy was induced by bezafibrate treatment after post-ovulatory aging. Collectively, these results suggest that bezafibrate beneficially affects against porcine post-ovulatory oocyte aging in porcine by its antioxidant property and mitochondrial protection.

• 대한치과보철학회지
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• 제38권6호
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• pp.757-764
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• 2000

Statement of the problem. All-ceramic post-and-core restorations offer a number of advantages compared with systems that use metal build-ups. In certain clinical cases, however, fractures at the joint between the post and core build-up have been reported. Purpose. The objective, therefore, is to improve the joint between the post and the core build-up. Material and methods. Three different methods were used to prepare all-ceramic post-and-core restorations; pressing IPS Empress core build-ups to CosmoPost zirconia posts, cement-ing IPS Empress core build-ups to CosmoPost zirconia posts and Celay-milling of zirconia blanks. A series of ten restorations was prepared for each of the three methods. The post-and-core complexes were tested to failure with the load applied perpendicular to the post axis. The load and deflection at fracture were recorded. Results. The highest breaking load and highest deflection were recorded for the cementing technique with values of 25.3 N and $394{\mu}m$, respectively, The corresponding values for the pressed core build-ups and the milled zirconia core build-ups were 22 N and $301{\mu}m$, and 13 N and $160{\mu}m$, respectively. All the differences are statistically significant (p=0.05). Regarding the load-dependence of the deflection, the cemented core build-ups again demonstrated the highest value with $15.5{\mu}m/N$. The difference in the values of $13.6{\mu}m/N\;and\;13{\mu}m/N$ recorded for the pressed-on and milled core build-ups, respectively, were statistically insignificant. Conclusion. In regard to the high fracture resistance of zirconia post, adhesive cementing the core build-up to the post offers a viable alternative to the conventional pressing technique. The elastic bond between the rigid high-strength zirconia post and the core build-up presents an additional advantage.

• 과학기술학연구
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• 제10권2호
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• pp.1-35
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• 2010

탈(脫)추격형 혁신은 기술만이 아니라 그 기술이 개발.활용되는 시장과 제도를 함께 창출시켜야 한다는 점에서 새로운 접근을 필요로 한다. 본 연구는 탈추격형 혁신의 개념과 핵심 과제들을 살펴보고, 탈추격형 혁신을 효과적으로 전개하기 위해서는 혁신정책과 산업 인력 노동 지역 금융 사회 등 관련 정책간의 연계 통합을 지향하는 '통합적 혁신정책(integrated innovation policy)'이 필요함을 주장하였다. 탈추격형 혁신 상황에서는 기술혁신활동과 시장/제도의 창출활동이 동시에 수행되면서 기술만이 아니라 그것을 개발하고 활용하는 사회의 구성이 함께 이루어진다. 기술과 사회의 동시 구성을 효과적으로 수행하기 위해서는 혁신정책과 교육 산업 노동 지역 사회 등이 서로 보완성을 갖도록 단편적인 정책영역을 넘어 다른 부문 정책까지 고려하는 통합적 혁신정책이 요구된다. 탈추격형 혁신을 위한 통합적 혁신정책 구현을 위해서는 장기 비전 창출과 광범위한 정책 연계 통합을 이끌어내는 전환가적 정부 역할이 요구된다. 이와 함께 제도, 환경, 인프라 전반을 통해 행동의 변화를 유도하는 시스템 혁신이 중요해진다. 이를 달성하기 위한 예로 예산을 통해 관련정책의 통합성을 확보할 수 있는 부처 간 통합예산제도를 도입하여 각 부처가 공동으로 프로그램을 설계 집행하는 방안을 검토할 필요가 있다. 또한 탈추격 상황이 갖는 고도의 불확실성에 대응하기 위해서는 전면적인 정책실행 보다 다양한 시범 사업을 바탕으로 한 소규모의 정책 실험을 수행해 나가야 한다. 소규모 실험의 성공을 바탕으로 점차 실험의 크기를 확대해 나가면서 관련 지식을 축적하고 정책의 불확실성을 점차 줄여갈 수 있다.

• 한국광학회지
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• 제29권2호
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• pp.64-69
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• 2018

본 논문에서는 양자시스템이 두 순수상태가 임의대로 혼합된 상태, 즉 2-계수 양자상태, 에 존재할 때 시스템이 포함하는 양자얽힘의 일반적인 하한값을 유도하고 양자얽힘검증 및 양자상태 분리가능성 판별 방법을 제안한다.

• Journal of Microbiology and Biotechnology
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• 제9권3호
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• pp.255-259
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• 1999

A 6.9-kb DNA fragment including the minimal Bacillus pasteurii urease gene cluster was subcloned into a high-copy-number plasmid vector, pUC19, and the recombinant B. pasteurii urease was overexpressed in Escherichia coli. The recombinant urease was purified 25.9-fold by using combinations of anion-exchange and gel-filtration chromatography followed by Mono-Q chromatography on a FPLC. N-terminal peptide sequencing analyses revealed that two distinct smaller peptide bands resolved on a 10-18％ gradient SDS-PAGE corresponded to UreA and UreB peptides, respectively. It was also shown that the ureB gene was translated from a GUG codon and the first methionine residue was post-translationally cleaved off. The native molecular weight of the recombinant urease was 176,000 and 2 nickel atoms were present per catalytic unit. pH stability studies of the purified enzyme showed that the recombinant Bacillus pasteurii urease is stable in alkaline pH range, which is similar to the enzyme of the evolutionarily related bacterium, Sporosarcina ureae.

• 한국운동역학회지
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• 제26권2호
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• pp.143-151
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• 2016

Objective: The purpose of this study was to investigate the effect of fatigue induction on ground reaction force (GRF) components, postural stability, and vertical jump performance in Taekwondo athletes. Method: Ten Taekwondo athletes (5 men, 5 women; mean age, $22.30{\pm}2.62years$; mean height, $174.21{\pm}9.20cm$; mean body weight, $67.28{\pm}12.56kg$) participated in this study. Fatigue was induced by a short period of strenuous exercise performed on a motorized treadmill. The analyzed variables included vertical jump performance, static stability (mediolateral [ML], center of pressure [COP], anteroposterior [AP] COP, ${\Delta}COPx$, ${\Delta}COPy$, and COP area), postural stability index values (ML stability index [MLSI], AP stability index [APSI], vertical stability index [VSI], dynamic postural stability index [DPSI]), and GRF components (ML force, AP force, peak vertical force [PVF], and loading rate). To analyze the variables measured in this study, PASW version 22.0 was used to calculate the mean and standard deviation, while a paired t-test was used to evaluate the pre- versus post-fatigue results. Pearson's correlation coefficients among variables were also analyzed. The statistical significance level was set at ${\alpha}$ = .05. Results: Vertical jump performance decreased significantly after the induction of fatigue, while AP COP, ${\Delta}COPx$, COP area, APSI, VSI, and DPSI increased significantly. PVF and loading rate increased significantly after the induction of fatigue, while the postural stability variables (AP COP, ${\Delta}COPy$, COP area, APSI, VSI, DPSI) were similarly correlated with GRF components (PVF, loading rate) after fatigue was achieved (r = .600, $R^2$ = 37%). Conclusion: These results suggest that the induction of fatigue can decrease postural stability and exercise performance of Taekwondo athletes during training and competition sessions.

• 한국인터넷방송통신학회논문지
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• 제15권1호
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• pp.37-43
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• 2015

웹에서 게시되는 이미지 콘텐츠에 대한 기술적 조치들은 화면캡처 방지와 같은 복제방지 방식과 워터마킹 삽입이나 특징점 정보를 통해 무단복제물을 검출 할 수 있는 추적 기술들이 존재한다. 그러나 화면캡처 방지 기술은 이미지 소스 URL이 노출될 경우에는 무단복제를 막을 수 없고 추적 기술은 불법행위를 적발하기 위한 후처리방식이기 때문에 불법복제를 원천적으로 차단하지 못한다는 단점이 존재한다. 또한 저작권 정보 표시기술을 이용할 경우 저작권 정보가 저작권보호 대상의 콘텐츠로부터 간단히 제거 될 수 있다는 단점이 있다. 본 논문에서는 웹 이미지 콘텐츠의 저작권정보를 쉽게 분리할 수 없게 하면서 허가된 사이트에서만 이미지 콘텐츠가 게시되어 열람될 수 있도록 하는 웹 이미지 콘텐츠 저작권 보호 시스템 모델을 제시한다.

• Molecules and Cells
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• 제40권2호
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• pp.151-161
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• 2017

Proper synaptic function in neural circuits requires precise pairings between correct pre- and post-synaptic partners. Errors in this process may underlie development of neuropsychiatric disorders, such as autism spectrum disorder (ASD). Development of ASD can be influenced by genetic factors, including copy number variations (CNVs). In this study, we focused on a CNV occurring at the 16p11.2 locus in the human genome and investigated potential defects in synaptic connectivity caused by reduced activities of genes located in this region at Drosophila larval neuromuscular junctions, a well-established model synapse with stereotypic synaptic structures. A mutation of rolled, a Drosophila homolog of human mitogen-activated protein kinase 3 (MAPK3) at the 16p11.2 locus, caused ectopic innervation of axonal branches and their abnormal defasciculation. The specificity of these phenotypes was confirmed by expression of wild-type rolled in the mutant background. Albeit to a lesser extent, we also observed ectopic innervation patterns in mutants defective in Cdk2, Gq, and Gp93, all of which were expected to interact with Rolled MAPK3. A further genetic analysis in double heterozygous combinations revealed a synergistic interaction between rolled and Gp93. In addition, results from RT-qPCR analyses indicated consistently reduced rolled mRNA levels in Cdk2, Gq, and Gp93 mutants. Taken together, these data suggest a central role of MAPK3 in regulating the precise targeting of presynaptic axons to proper postsynaptic targets, a critical step that may be altered significantly in ASD.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제16권6호
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• pp.712-716
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• 2010

본 논문에서는 레퍼런스 시퀀스에 기가 시퀀싱데이터를 매핑하여 얻어지는 커버리지 데이터를 이용한 모양 기반의 단위반복변이 영역 (CNVR) 추출 방식을 제안한다. 제안하는 CNVR 검색 알고리즘은 후보 영역 추출 단계와 후처리 단계로 이루어진다. 후보 영역 추출 단계에서는 추출하고자 하는 CNV의 모양을 입력 변수로 조절하여 다양한 높이 및 크기를 갖는 CNV 후보 영역을 추출한다. 다음, 후처리 단계에서는 레퍼런스 시퀀스와 기가 시퀀싱 데이터에 포함되어 있는 시퀀싱 에러 문제를 보완하기 위하여, 레퍼런스 시퀀스의 에러 영역 보정, GC-content 영역 보정 등의 정제 과정을 거친 후, 최종 CNVR을 추출한다. 제안된 방식의 유용성을 보이기 위하여 "1000 게놈 프로젝트"에 의하여 공개된 실 데이터를 이용한 다양한 실험을 수행하였으며, DGV를 이용하여 추출된 CNVR의 정확도를 검증하였다. 실험 결과에 의하면 제안된 방식은 HLA 영역에 존재하는 반복되거나 결실되는 다양한 모양의 CNV를 효율적으로 검출하였다.