• 제목/요약/키워드: Positive and negative sequence

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Polyphasic Assignment of a Highly Proteolytic Bacterium Isolated from a Spider to Serratia proteamaculans

  • Kwak, Jang-Yul;Lee, Dong-Hun;Park, Youn-Dong;Kim, Seung-Bum;Maeng, Jin-Soo;Oh, Hyun-Woo;Park, Ho-Yong;Bae, Kyung-Sook
    • Journal of Microbiology and Biotechnology
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    • 제16권10호
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    • pp.1537-1543
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    • 2006
  • A bacterial strain named HY-3 that produces a highly active extracellular protease was isolated from the digestive tract of a spider, Nephila clavata. The bacterium was a Gram-negative, oxidase-negative, catalase-positive, nonhalophilic, nitrate-reducing, facultative anaerobe. Transmission and scanning electron microscopies demonstrated that the isolate was non-spare-forming, straight, rod-shaped, and motile by peritrichous flagella. The G+C content of the DNA was 57.0 mol%. The isoprenoid quinone type was ubiquinone with 8 isoprene units (Q-8). The morphological and biochemical characteristics including the predominant fatty acid and phospholipids profiles placed the isolate HY-3 in the family Enterobacteriaceae. Further biochemical characterization and phylogenetic studies including determination of an almost complete 16S ribosomal DNA sequence suggested that the bacterium was closely related to the genus Serratia. DNA-DNA hybridization analysis revealed that this extracellular protease-producing strain belongs to Serratia proteamaculans, which is also known far its association with insects.

저속영역에서 교류전동기의 정확한 자속추정을 위한 전류측정오차 보상 (Correction on Current Measurement Errors for Accurate Flux Estimation of AC Drives at Low Stator Frequency)

  • 조경래;석줄기
    • 전력전자학회논문지
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    • 제12권1호
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    • pp.65-73
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    • 2007
  • 본 논문은 1-Hz의 낮은 고정자 전류 주파수에서도 동작하는 순수적분 기반의 자속추정을 위한 온라인 전류측정 오차 보상방법을 제안한다. 오프셋 전류와 변환이득오차에 의한 역상분 전류 성분은 상태관측기를 이용하여 제거하고, 동시에 변환이득오차에 의한 역상분 전류는 동기좌표계에서 영구자석에 의하여 발생된 q축 자속을 기준모델에 의한 값과 추정된 자속에 의한 값 사이의 차이에 의하여 보상한다. 이 보상기는 PI제어기를 이용하여 두 값 사이의 오차가 0이 되도록 제어한다. 또한 적분기 초기값 오차 및 관측기의 전동기 상수 오차에 의한 잔여오차 보상방법도 제안하였다. 타당성을 입증하기 위하여 1.1-kW 영구자석형 동기전동기(PMSM)에 제안된 보상 방법을 구현하여 다양한 실험을 수행하였다.

논에서 분리한 메탄산화세균 Methylomonas sp. SM4의 특성과 메탄올 생합성 (Characterization and Methanol Biosynthesis of a Methane-Oxidizing Bacterium, Methylomonas sp. SM4, Isolated from Rice Paddy Field Soil)

  • 박성민;;김시욱
    • KSBB Journal
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    • 제32권2호
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    • pp.124-132
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    • 2017
  • A methane-oxidizing bacterium was isolated from rice paddy field soil around Jeollanam-do province, Korea, and characterized. The isolate was gram-negative, orange pigmented and short rod ($1.1-1.2{\times}1.6-1.9{\mu}m$). It was catalase and urease-negative but oxidase-positive. The strain utilized methane and methanol as sole carbon and energy sources. It had an ability to grow with an optimum pH 7.0 and an optimum growth temperature $30^{\circ}C$. The strain was resistant to antibiotic polymyxin B but sensitive to streptomycin, kanamycin, ampicillin, chloramphenicol and rifampicin. The isolate required copper for their growth with concentration range of $2-25{\mu}M$, with an optimum of $10{\mu}M$. Under optimal culture condition, specific cell growth rate and generation time were found to be $0.046hr^{-1}$ and 15.13 hr, respectively. Phylogenetic analysis based on 16S rDNA sequences indicated that the strain formed a tight phylogenetic lineage with Methylomonas koyamae with a value of 99.4% gene sequence homology. So, we named the isolate as Methylomonas sp. SM4. 8.6 mM methanol was accumulated in the reaction mixture containing 70 mM sodium formate and 40 mM $MgCl_2$ (MDH inhibitor) under atmosphere of methane:air (40:60) mixture for 24 hr at $30^{\circ}C$.

Plasma Real Time-Quantitative Polymerase Chain Reaction of Epstein-Barr Virus in Immunocompetent Patients with Hepatitis

  • Hong, Ji-Hye;Bae, Yon-Jung;Sohn, Joon-Hyung;Ye, Byung-Il;Chun, Jin-Kyong;Kim, Hwang-Min
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • 제15권1호
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    • pp.38-43
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    • 2012
  • Purpose: Epstein-Barr virus (EBV) hepatitis is a usually asymptomatic and self-limiting disease in immunocompetent patients. However, the range of severity is wide, and the serological diagnosis is typically difficult until the convalescent phase. Thus, we examined the value of plasma EBV DNA real-time quantitative polymerase chain reaction (RT-qPCR) in EBV hepatitis for the timely diagnosis and the relationship between EBV viral load and clinical severity. Methods: Sixty samples were confirmed as having EBV infection by RT-qPCR with the EBV BALF5 gene sequence. We examined the clinical characteristics of EBV hepatitis by reviewing medical records. Results: The median total duration of fever was 8 days (range: 0-13 days). The mean peak value of aspartate aminotransferase (AST) was $241{\pm}214$ U/L, and the mean peak value of alanine aminotransferase (ALT) was $298{\pm}312$ U/L. There was no correlation between the serum levels of liver enzyme and plasma EBV DNA titer ($p$=0.1) or between median total duration of fever and EBV DNA titer ($p$=0.056). The median age of the EBV VCA IgM-negative group was lower compared with the EBV VCA IgM-positive group in EBV hepatitis (2 years vs. 6 years, $p$=0.0009). Conclusion: The severity of EBV hepatitis does not correlate with circulating EBV DNA load according to our data. Furthermore, we suggest that plasma EBV PCR may be valuable in young infants in whom the results of serology test for EBV infection commonly are negative.

신규 프로바이오틱스로서 Peanibacillus sp. BCNU 5016의 특성 (Characteristics of Paenibacillus sp. BCNU 5016 as a Novel Probiotic)

  • 최혜정;김동완;주우홍
    • 생명과학회지
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    • 제24권2호
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    • pp.161-166
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    • 2014
  • 신규 프로바이오틱스 균주로서 사용하기 위하여 한국의 염장 발효 생선가공품인 젖갈로부터 Paenibacillus sp. 균주들을 탐색하였다. 이들 균주들 중에서 BCNU 5016은 그람양성균으로 gelatinase와 urease를 생산하지 않는 전형적인 Paenibacillus sp. 균주로 확인되었다. 이 균주는 16S rDNA 염기서열 분석을 바탕으로 계통적으로 Paenibacillus polymyxa의 근연종임이 확인되었다. Paenibacillus sp. BCNU 5016은 내산성 실험을 통해 pH 2.5에서 3h 배양 후에도 91.89%까지 생존함이 확인되었고, 담즙산에도 뛰어난 내성이 있음이 확인되었다. 또한 BCNU 5016은 자가응집능, 공동응집능 및 소수성 능력으로 볼 때 우수한 장점막 부착능력을 가지고 있는 것으로 판단된다. 그러므로 Paenibacillus sp. BCNU 5016은 프로바이오틱스 균주로서 우수한 잠재력을 가지고 있음을 확인할 수 있었다.

β-Galactosidase Gene of Thermus thermophilus KNOUC112 Isolated from Hot Springs of a Volcanic Area in New Zealand: Identification of the Bacteria, Cloning and Expression of the Gene in Escherichia coli

  • Nam, E.S.;Choi, J.W.;Lim, J.H.;Hwang, S.K.;Jung, H.J.;Kang, S.K.;Cho, K.K.;Choi, Y.J.;Ahn, J.K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제17권11호
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    • pp.1591-1598
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    • 2004
  • To isolate the $\beta$-galactosidase producing thermophilic bacteria, samples of mud and water were collected from hot springs of avolcanic area near Golden Springs in New Zealand. Among eleven isolated strains, the strain of KNOUC112 produced the highest amounts of $\beta$-galactosidase at 40 h incubation time (0.013 unit). This strain was aerobic, asporogenic bacilli, immobile, gram negative, catalase positive, oxidase positive, and pigment producing. Optimum growth was at 70-72$^{\circ}C$, pH 7.0-7.2, and it could grow in the presence of 3% NaCl. The main fatty acids of cell components were iso-15:0 (30.26%), and iso-17:0 (31.31%). Based on morphological and biochemical properties and fatty acid composition, the strain could be identified as genus Thermus, and finally as Thermus thermophilus by phylogenetic analysis based on 16S rRNA sequence. So the strain is designated as Thermus thermophilus KNOUC112. A gene from Thermus thermophilus KNOUC112 encoding $\beta$-galactosidase was amplified by PCR using redundancy primers prepared based on the structure of $\beta$-galactosidase gene of Thermus sp. A4 and Thermus sp. strain T2, cloned and expressed in E. coli JM109 DE3. The gene of Thermus thermophilus KNOUC112 $\beta$-galactosidase(KNOUC112$\beta$-gal) consisted of a 1,938 bp open reading frame, encoding a protein of 73 kDa that was composed of 645 amino acids. KNOUC112$\beta$-gal was expressed as dimer and trimer in E. coli JM109 (DE3) via pET-5b.

해양에서 분리한 Exiguobacterium sp. SC2-1의 항산화 활성 및 특성 (Antioxidant Activity and Characterization of Exiguobacterium sp. SC2-1 Isolated from Sea Water)

  • 김만철;박근태;손홍주;최우봉;허문수
    • 미생물학회지
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    • 제41권1호
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    • pp.74-80
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    • 2005
  • 제주도 연안의 해수로부터 항산화물질을 생산하는 세균을 분리하였으며 항산화물질의 특성을 조사하였다. 분리된 항산화 생성 균주는 그람양성의 통성혐기성균으로 운동성을 가진 단간균이었으며 생육시 NaCl를 필요로 하였다. 분리균주는 형태학적, 배양적, 생화학적 특성 및 165 rDNA 염기서열 분식결과 Exiguobacterium 속으로 동정되어 Ekiguobaoteriurn sp. SC2-1로 명명하였다. 항산화물질 생산균주 배양액의 radical 소거활성은 DPPH (1,1-diphenyl-2-picrylhydrazyl) 법을 사용하였으며, 항산화물질 생성 최적조건은 $25^{\circ}C,\;NaCl\;4\%,\;pH\;6\~8$ 이었으며, 최적 탄소원은 $1\%$ maltose였다. 배양액의 hydroxyl radical 소거활성은 $73\%$였으며 superoxide radical 소거활성은 $35\%$를 나타내었다.

약수에서 분리한 Yersinia pseudotuberculosis의 병원성과 16S rDNA 분석에 의한 분자학적 분류 (Molecular Taxonomy based on 16S rDNA Analysis and Pathogenicity of Yersinia pseudotuberculosis Isolated from Spring Waters)

  • 이영기;최성민;오수경;이강문;염곤
    • 미생물학회지
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    • 제37권1호
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    • pp.9-14
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    • 2001
  • 서울시내 25개 자치구에 산재한 약수터에서 5주의 Yersinia pseudotuberculosis를 분리하여 생화학적 특성, 병원성의 유무 및 16S rDNA 분석을 실시하였다. 분리된 Y. pseudotuberculosis는 모두 병원성 유전자인 inv를 소유하고 있었으며, 16S rDNA를 증폭한 후 염기서열을 분석하여 NCBI Genbank에 등록된 다른 Yersinia 속 및 장내세균 등과 비교해 본 결과 Yersinia 속 등과는 97.5%에서 100%의 높은 상동성(Similarity)을 나타내었고, 다른 장내세균 등과는 93.0%에서 95.1%의 낮은 상동성을 나타내었다. 165 rDNA 염기서열을 기초로 계통수를 작성한 결과 크게 3개의 cluster를 형성하였는데 특히 Y.enterocolitica (Z49830)은 Y.pseudotuberculasis (Z21939)와의 상동성(97.7%)보다 Y.intermedia (X75279)와의 상동성(97.9%)이 더 높게 나타났으며, E. coli (Z83205)는 Proteus vulgaris (AJ233425) 와의 상동성(93.2%)보다 Salmonella enteritidis (U90318)와의 상동성(97.7%)이 더 밀접한 연관성을 나타내었다.

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Ramlibacter terrae sp. nov. and Ramlibacter montanisoli sp. nov., Isolated from Soil

  • Khan, Shehzad Abid;Kim, Hyung Min;Baek, Ju Hye;Jung, Hye Su;Jeon, Che Ok
    • Journal of Microbiology and Biotechnology
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    • 제31권9호
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    • pp.1210-1217
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    • 2021
  • Two gram-negative, catalase-positive, strictly aerobic, and white colony-forming bacteria, strains H242T and B156T, were isolated from soil in South Korea. Cells of strain H242T were oxidase-positive and non-motile short rods, while those of strain B156T were oxidase-negative and long non-motile rods. Ubiquinone-8 was identified as the sole isoprenoid quinone in both strains. C16:0, cyclo-C17:0, andsummed feature 3 (C16:1 ω7c and/or C16:1 ω6c) and phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol were identified in both strains as the major cellular fatty acids and polar lipids, respectively. The DNA G+C contents of strains H242T and B156T were 69.4 mol% and 69.3 mol%, respectively. Phylogenetic analyses based on 16S rRNA and 92 concatenated core gene sequences revealed that strains H242T and B156T formed distinct phylogenic lineages from other Ramlibacter type strains. The DNA-DNA hybridization (DDH) value between strains H242T and B156T was 24.6%. Strains H242T and B156T were most closely related to Ramlibacter ginsenosidimutans BXN5-27T and Ramlibacter monticola G-3-2T with 98.4% and 98.6% 16S rRNA gene sequence similarities, respectively. Digital DDH values between strain H242T and R. ginsenosidimutans and between strain B156T and R. monticola were 23.5% and 26.1%, respectively. Phenotypic, chemotaxonomic, and molecular analyses indicated that strains H242T and B156T represent two novel species of the genus Ramlibacter, for which the names Ramlibacter terrae sp. nov. and Ramlibacter montanisoli sp. nov., respectively, are proposed. The type strains of R. terrae and R. montanisoli are H242T (=KACC 21667T=JCM 33922T) and B156T (=KACC 21665T=JCM 33920T), respectively.

기독병원과 일반병원의 조직문화 특성에 따른 조직성과 분석 (Analysis on Organization Performance Based on Hospital Culture)

  • 김운신;남은우
    • 한국병원경영학회지
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    • 제4권2호
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    • pp.242-265
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    • 1999
  • Our study attempt is to see organizational performance according to the differences between types of hospital cultures. To determine theoretical relationship between the organizational culture and the performance, we select two hospitals in Pusan which are different in the purposes and shapes of establishment. We surveyed their members on a questionnaire based on the type of the organizational culture of the two institutions, analyzed, and review its organizational effectiveness. For the survey with questionnaires, which had been preliminary studied to raise its validity, question forms were distributed to 528 persons in April, 1999 based on the self-responses and recollected within 48 hours. The recollection rate was high(89.96%) and the quantity of questionnaires used for our final analysis was 430(81.44%). The Cronbach Coefficient Alpha of the questionnaires was 0.742. Regarding statistical techniques for analysis of the written materials, dispersion analysis(ANOVA) was adapted to test the organizational effectiveness of the two hospitals having the different organizational cultures, and Pearson Correlation was applied to determine correlations was among all variables. T-test was performed to test organizational effectiveness based on the differences in the extent of sharing the culture, organizational committment and work satisfaction between the two health institutions. From our analysis, we obtain the following conclusions. First, concerning with organizational culture of the two hospital, one of which is a christian hospital and the other is a private foundation hospital, the former is conservative and human-oriented but the latter focuses on renovation and accomplishment. Second, the private establishment has a relatively higher organizational effectiveness that the religious hospital as a result of analyzing the extent of sharing culture, organizational committment and work satisfaction. Third, it has been found that the correlations between the extent of the sharing culture and the organizational committment, the extent and work satisfaction, and the committment and the satisfaction are respectively positive influencing organizational effectiveness, especially work satisfaction. Fourth, cultural factors by which the christian hospital is affected more positively including human relations among its members, belief, its idea of establishment, tradition, work responsibility, power, and wage. On the other hand, factors such as director's leadership, personnel management, wage, hospital regulations and department managers' management ability have been seen as negative influences in order. And fifth, for the private foundation hospital human relations among its members, wages, work responsibility, director's leadership and department managers' management ability were positive in their sequence while wages, personnel management, hospital regulations, welfare and department managers' management ability were considered as negative influences in order. As these results of this study, the higher extent of sharing organizational culture, the more increasing in both organization committment and work satisfaction, the higher the effectiveness. Although it was somewhat difficult to generalize the results whose subjects were the two hospitals only, it was obvious that organizational culture was an important influential factor of organizational effectiveness. It is questionable that the extent of sharing organizational culture, organizational committment and work satisfaction as variables affecting the effectiveness have their validity, but this study has its significance in that it provided an approaching to evaluate the organizational culture of individual hospitals making allowances for such variables related to the general activities in its hospital. We hope the results of the study could be useful for the managerial strategies of the institutions.

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