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http://dx.doi.org/10.5352/JLS.2014.24.2.161

Characteristics of Paenibacillus sp. BCNU 5016 as a Novel Probiotic  

Choi, Hye Jung (Department of Biology and Interdisciplinary Program for Biotechnology, Changwon National University)
Kim, Dong Wan (Department of Microbiology, Changwon National University)
Joo, Woo Hong (Department of Biology and Interdisciplinary Program for Biotechnology, Changwon National University)
Publication Information
Journal of Life Science / v.24, no.2, 2014 , pp. 161-166 More about this Journal
Abstract
We screened Paenibacillus sp. strains from Jeotgal, a Korean salted and fermented fish product, for use as a novel probiotic. Among these Paenibacillus sp. isolates, BCNU 5016 was a typical Paenibacillus sp. strain that showed gram-positive, gelatinase-negative, and urease negative activity. On the basis of 16S rDNA sequence comparisons, BCNU 5016 was most closely related phylogenetically to P. polymyxa. When Paenibacillus sp. BCNU 5016 was subjected to the acid tolerance test, this strain showed 91.89% survival after 3 h culture at pH 2.5. Paenibacillus sp. BCNU 5016 also showed excellent bile acid tolerance. Furthermore, its auto-aggregation, coaggregation, and hydrophobic capacities suggest that BCNU 5016 had the capacity to adhere well to the intestinal tract. We conclude that Paenibacillus sp. BCNU 5016 has excellent potential as a probiotic.
Keywords
Acid tolerance; bile acid tolerance; hydrophobic capacity; Paenibacillus sp.; probiotic;
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1 Hong, H. A., Duc, L. H. and Cutting, S. M. 2005. The use of bacteria spore formers as probiotics. FEMS Microbiol Rev 29, 813-835.   DOI   ScienceOn
2 Saito, N. and Nei, M. 1987. The neighbor-joining method, a new method for reconstructing phylogenetic trees. Mol Biol Evol 79, 426-434.
3 Salkinoja-Salonen, M. S., Vuorio, R., Andersson, M. A., Kampfer, P., Andersson, M. C., Honkanene-Buzalski, T. and Scoging, A. C. 1999. Toxigenic strains of Bacillus licheniformis related to food poisoning. Appl Environ Microbiol 65, 4637-4645.
4 Shin, H. J., Bang, J. H., Choi, H. J., Kim, D. W., Ahn, C. S., Jeong, Y. J. and Joo, W. H. 2012. Probiotic potential of indigenous Bacillus sp. BCNU 9028 isolated from Meju. J Life Sci 22, 605-612.   과학기술학회마을   DOI
5 Sneath, P. H. A. 1986. Endospore-forming gram-positive rods and cocci, pp. 1104-1139. In: Sneath, P. H. A., Mair, N. S., Sharpe, M. E. and Holt, J. G. (eds.), Bergey's Manual of Systematic Bacteriology. Williams & Wilkins, Baltimore, Maryland, USA.
6 Wang, G. X., Wang, Y., Wu, Z. F., Jiang, H. F., Dong, R. Q., Li, F. Y. and Liu, X. L. 2011. Immunomodulatory effects of secondary metabolites from thermophilic Anoxybacillus Kamchatkensis XA-1 on carp, Cyprinus carpio. Fish Shellfish Immunol 30, 1331-1338.   DOI
7 Choi, S. S., Kang, B. Y., Chung, M. J., Kim, S. D., Park, S. H., Kim, J. S., Kang, C. Y. and Ha, N. J. 2005. Safety assessment of potential lactic acid bacteria bifidobacterium longum SPM1205 isolated from healty Koreans. J Microbiol 43, 493-498.
8 Cole, C. B., Fuller, R. and Carter, S. M. 1989. Effect of probiotic supplements of Lactobacillus acidophilus and Bifidobacterium adolescentis on ${\beta}$-glucosidase and ${\beta}$-glucuronidase activity in the lower gut of rats associated with a human faecal flora. Microbiol Ecol Health Dis 2, 223-225.   DOI
9 Doly, R. J. and Rosenberg, M. 2000. Effect of probiotic bacteria on diarrhea, lipid metabolism and carcinogenesis. Am J Clin Nutr Rev 71, 405-411.   DOI
10 Ishibashi, N. and Yamazaki, S. 2001. Probiotics and safety. Am J Clin Nutr 73, 465S-470S.   DOI
11 Kim, S. M., Lee, K. H., Lee, N. K., Kim, C. J. and Paik, H. D. 2004. Antagonistic activity of polyfermenticin SCD against Helicobacter pylori KCTC 2948. J Microbiol Biotechnol 14, 148-151.   과학기술학회마을
12 Mercenier, A., Pavan, S. and Pot, B. 2003. Probiotics as biotherapeutic agents: present knowledge and future prospects. Curr Pharm Des 8, 99-100.
13 Ouwehand, A. C., Kirjavainen, P. V., Shortt, C. and Salminen, S. 1999. Probiotics: mechanisms and establish effects. Int Dairy J 9, 43-52.   DOI   ScienceOn
14 Park, H. S., Lee, S. H. and Uhm, T. B. 1998. Selection of microorganisms for probiotics and their characterization. J Koerean Soc Food Sci Nutr 27, 433-440.   과학기술학회마을
15 Gilliland, S. E., Staley, T. E. and Bush, L. J. 1984. Importance of bile tolerance of Lactobacillus acidophilus used as a dietary adjunct. J Dairy Sci 67, 3045-3051.   DOI   ScienceOn