Purpose: This study investigated the factors affecting spirituality in nursing students. Method: A total of 152 nursing students participated in the study. Data collection was conducted from October to November 2018. The data were analyzed using descriptive statistics, t-tests, analysis of variance, Pearson's correlation coefficient, and multiple stepwise regression via SPSS WIN 25.0. Results: The mean scores for empathy, gratitude, positive affect, negative affect, and spirituality were all average. However, there were significant differences in mean scores for spirituality according to satisfaction with major (F=5.88, p=.003), study grade (F=10.17, p<.001). Spirituality was found to be correlated with fantasy (r=.17, p<.001), empathic concern (r=.41, p<.001), perspective taking (r=.42, p<.001), personal distress (r=-.31, p<.001), gratitude (r=.65, p<.001), positive affect (r=.52, p<.001), and negative affect (r=-.30, p<.001). Significant predictors of spirituality included gratitude (${\beta}=.38$, p<.001), study grade (${\beta}=.34$, p=.009), positive affect (${\beta}=.29$, p<.001), and perspective taking (${\beta}=.16$, p=0.37), one of the sub-dimensions of empathy. Conclusion: To improve spirituality among nursing students, nursing educators should develop educational intervention programs to promote gratitude, study grades, positive affect, and perspective taking.