• 제목/요약/키워드: Porphyra seriata

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Life History of Porphyra seriata Kjellman (Bangiales, Rhodophyta) from Korea in Laboratory Culture

  • Kim, Nam-Gil;Notoya, Masahiro
    • ALGAE
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    • 제19권4호
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    • pp.303-309
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    • 2004
  • The laboratory culture study of Porphyra seriata Kjellman from Korea was conducted at different conditions of temperatures (5, 10, 15, 20, 25 and 30${^{\circ}C}$), photon flux densities (10, 20, 40 and 80 $\mu$mol $^{-2}s^{-1}$) and photoperiods (14L: 10D and 10L:14D). Conchocelis filaments grew fast at 15-20${^{\circ}C}$ and 20-80 $\mu$mol $^{-2}s^{-1}$ under both photoperiods. Concho sporangial branches were produced at 5-25${^{\circ}C}$, and abundant when the conchocelis filaments were cultured at higher temperatures of 20-25${^{\circ}C}$ under both photoperiods. Foliose thalli grew well at 15-20${^{\circ}C}$ under 10L:14D and at 20${^{\circ}C}$ under 14L:10D. At 30${^{\circ}C}$, the foliose thallus failed to survive. No archespores were observed at any culture conditions. Spermatangia and zygotosporangia were formed in squarish patches at the upper marginal portion of mature thalli. Anatomical examination revealed that the mature spermatangia were 64 (a/4, b/2, c/8) and 128 (a/4, b/4, c/8), and that of zygotosporangium was 16 (a/2, b/2, c/4) according to the Hus' formula.

홍조류인 한국산 김종에서의 염색체 DNA 분리방법 (Rapid and Efficient Purification of Nucleic Acids from the Macroalga Porphyra(Rhodophyta))

  • 류태형;최학선;최경희;이춘환
    • 한국식품영양과학회지
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    • 제27권6호
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    • pp.1166-1172
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    • 1998
  • A method for the isolation and purification of DNA from a red algae, Porphyra was innovated. The innovation of the method consists mainly of three steps that include sodium acetate treatment, chloroform extraction, and 0.2 volume isopropanol precipitation step. The sodium acetate treatment was designed to remove polysaccharide contamination, and the isopropanol step to remove proteins and salts contaminents. Genomic DNA,s of several species(for example, P. tenera, P. yezoensis, P. seriata, and P. pseudolinearis) was successfully isolated by the innovated method. The amount of DNA purified from one g of sample material with the innovated method was 53 g in average. The resulting DNA was characterized to include high molecular weight and showed no nuclease activity. The DNA was pure enough to be digested directly by various restriction enzymes without any difficulties. Porphyra DNA was pure enough and adequate for amplification reaction through the polymerase chain reaction (small nuclear rDNA PCR amplification).

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Cryopreservation of Sporothalli of the Genus Porphyra (Bangiales, Rhodophyta) from Korea

  • Jo, Young-Hyun;Kang, Sung-Pil;Seo, Tae-Ho;Choi, Sung-Je;Kho, Kang-Hee;Kuwano, Kazuyoshi;Saga, Naotsune;Kim, Min-Yong;Shin, Jong-Ahm
    • ALGAE
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    • 제18권4호
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    • pp.321-331
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    • 2003
  • Cryopreservation of sporothalli of the red alga Porphyra (P. seriata, P. yezoensis, P. tenera, P. pseudolinearis and P. dentata) was performed by the two-step cooling method in liquid nitrogen. The algal samples were suspended in various cryoprotective solutions, and slowly cooled to -40$^{\circ}C$ in 4 hours using a programmed freezer. After the first slow cooling the suspensions with cryoprotectants were immediately immersed in liquid nitrogen. The suspension from the programmed freezer was thawed quickly by agitation of the vial in a water bath at 40°C. When ice in the suspension of cryogenic vial was mostly melted, the vial was transferred to an ice bath for complete melting of the residual ice. The cryoprotectants in the vial were washed off by gradual dilution with seawater. The viability of the cell was assessed with neutral red staining. The viability of Porphyra samples ranged 54.6-70.9% when the mixed suspension of 10% dimethylsulfoxide and 0.5 M sorbitol in 50% seawater used as a cryoprotectant.

돌김 건제품의 함질소 엑스성분에 관한 연구

  • 박춘규;강태중;정규진
    • 한국어업기술학회:학술대회논문집
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    • 한국어업기술학회 2001년도 춘계 수산관련학회 공동학술대회발표요지집
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    • pp.132-133
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    • 2001
  • 돌김류에는 잇바디돌김 (미역김, Porphyra dentata), 둥근돌김 (P. suborbiculata), 긴잎돌김 (P. pseudolinearis), 모무늬돌김 (P. seriata) 등이 있다 (Kang and Ko, 1997). 김의 맛과 밀접한 관계가 있는 함질소엑스성분에 관한 연구는 유리아미노산 (Noda et al., 1975: Saito., 1975; Yoshie et al., 1993, Araki et al., 1996, 1997; Sakai and Kasai, 2000), ATP관련 화합물 (Fujii, 1967; Ooyama et al., 1968; Tashiro et al.,1983,1991; Araki et al.,1996) 등이 있다. (중략)

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한국산 양식김의 종류 (SPECIES OF CULTIVATED PORPHYRA IN KOREA)

  • 강제원
    • 한국수산과학회지
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    • 제3권2호
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    • pp.77-92
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    • 1970
  • Ueda, in the course of his systematic work on the lavers, Porphyra, in Japan and Korea in 1932, mentioned that most of the cultivated Porphyra belong to Porphyra tenera Kjellman. Then he, dividing the species into two forms, f. typica and f. kjellmani, put Korean cultivated Porphyra under the latter. From the 1930s to the early 1940s, Fujikawa, Kaneko and others worked on Physiological experiments or cultivational experiments of Porphyra in the culture-bed, but there was no mention about the cultivated Porphyra species. However, many fishermen generally recognize that the characteristics of cultivated Porphyra vary depending on their habitat or the picking season, and it is considered that these differences are due to the varieties of the species which are well adaptable to various environments. Recently, I have become aware of the predominant occurrence of P. yezoensis Ueda in most culture-beds of Korea as in the Tokyo Bay or other places in Japan. At present, since artificial seeding for the cultivation of Porphyra with Conchocelis has been carried out and peculiar species can be cultured, a study of the species of cultivated Porphyra has become an important subject. I collected the specimens from a number of culture-beds which are located in the legions shown in fig. 1 from January, 1968 to May, 1970 and found that there are five species, P. tenera Kjellman, P. yezoensis Ueda, P. kuniedai Kurogi, P. seriata Kjellman and P. suborbiculata Kjellman. Among them, P. kuniedai was treated as a round-type, a form of P. tenera, by Kunieda (1939) and Tanaka (1952) and the occurrence of this form is generally recognized by most fishermen. At present, as mentioned above, the most dominant species of cultivated Porphyra is P. yezoensis but the cultivation of P. tenera is restricted to certain culture-beds or the early half of the cultivation period. P. kunieda appears as a mixed species throughout most of the culture-beds, particulary in the later half of the period, while when it was picked in January it appeared dominantly in a place such as Gum-Dang where the 'Bal', splitted bamboo piece mat, was settled during the last of September. This is the first seeding process. The latter two species, P. suborbiculata and P. seriata appear frequently but in small amounts in the later half of the period particulary in the western region of the southern coast. However, it can not be ascertained when P. yezoensis becomes predominant, because specimens have not been available up until recent years but the process can be described as follows: We commonly recognize the ecological characteristics of P. tenera as follows; First, the conchospores of the species develop earlier and the period of its discharge is shorter than those of P. yezoensis; second, the microscopical buds discharge neutral spores which develop into new buds directly and buds develop repeatedly through a short period. Consequently, according to such above ecological characteristics, the species can grow thick on the 'Bal' exclusively. However, buds may disappear when they are harmed by disease such a 'infection by certain parasites or by other unusual environmental conditions. Thus P. yezoensis are enabled to grow on the 'Bal' instead of the former species since they not only develop later than the former but also macroscopical fronds discharge the neutral spore throughout the period from October to May. Likewise, if any disease appears in the culture-bed ill the later half of the period, the former is more severely damaged than the latter because the former have less resistance to the disease than the latter. Thus fewer frond survive and fewer carpospores which are the origin of the next generation can be discharged. However the latter by their nature can continue growing until early summer. In the case of the culture-bed where the above phenomenon occurs repeatedly P. yezoensis gradually may become the dominant species among cultivated Porphyra. In support of the validity of this process we find that according to the description and the plate of Wada (1941), P. tenera, P. yezoensis and P. kuniedai grow together in the culture-bed at the mouth of the Nakdong River where P. yezoensis occurs predominantly and mixed with P. kuniedai.

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Effects of Salinity on Chlorophyll Fluorescence from Porphyra Thalli and Comparison of Species with Different Intertidal Distribution

  • Hong Dang Diem;Kim Tae Hoon;Hwang Mi Sook;Chung Ik Kyo;Lee Choon-Hwan
    • Fisheries and Aquatic Sciences
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    • 제1권1호
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    • pp.122-128
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    • 1998
  • Characteristic changes in chlorophyll fluorescence from thalli of red alga, Porphyra, under high salt stress and during subsequent recovery were investigated, and the differences in the sensitivity to the stress among four species of Porphyra with different intertidal distributions were compared. By the treatment of NaCl with $9\%$ or higher concentrations, photochemical efficiency (Fv/Fm) decreased in a biphasic pattern: a rapid decrease was observed within 1­3 h and followed by a slow decline. The decrease of Fv/Fm was mainly due to the increase of Fo without significant increases of Fm. When the thalli treated with $15\%$ NaCl for 6 h were returned to natural sea water for recovery, the increase of Fv/Fm also showed a biphasic pattern: a rapid increase of Fv/Fm was observed within 2 h and followed by a slow increase. Differences in the sensitivity to salt stress among the four species could be found during recovery after the treatment of severe salt stress. After the treatment of $20\%$ NaCl for 6 h, Fv/Fm decreased below 0.3 in all of the four Porphyra species, and the species living in upper parts of the intertidal zone (P. suborbiculata and P. pseudolinearis) could recover better compared with the species in lower parts of the intertidal zone (P. seriata and P. yezoensis), during recovery for 24 h. The species collected from the coast of the South Sea seemed to be more tolerant than those in the East Sea.

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남서해역에서 양식되는 방사무늬김(Porphyra yezoensis Ueda)의 색소조성과 농도에 영향을 미치는 해양환경 특성 (Relationship between Environmental Characteristics and Pigment Composition and Concentrations of Porphyra yezoensis Ueda in the Southwestern Coast of the Korean Peninsula)

  • 김정배;이원찬;홍석진;심정희;박정임;박지혜;이유진
    • 환경생물
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    • 제30권3호
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    • pp.200-209
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    • 2012
  • 서식 환경에 따른 양식 김의 종류 및 광합성 색소 조성과 농도에 대하여 2011년 1월부터 3월까지 김 양식이 이루어지고 있는 남서해역에서 조사를 하였다. 수온의 범위는 $3.0{\sim}11.3^{\circ}C$이었고, 염분의 범위는 32.7~34.7으로 연안과 가까운 정점 1과 6에서 낮게 나타났다. 해수 중 DIN의 농도 범위는 $1.73{\sim}12.84{\mu}M$, DIP의 농도 범위는 $0.07{\sim}0.67{\mu}M$이었고, 규산염의 농도 범위는 $4.93{\sim}18.29{\mu}M$이었다. 해수 중 Chl a 농도 범위는 $0.41{\sim}9.14{\mu}g\;L^{-1}$로서 1월의 정점 1에서 가장 높았다. 해수 중 조류색소 조성은 규조류 (diatoms)의 marker 색소인 fucoxanthin이 $0.06{\sim}3.41{\mu}g\;L^{-1}$로 우점하였고, 정점 1에서 $3.41{\mu}g\;L^{-1}$로 1월에 가장 높았다. 용존 무기질소 농도는 모든 정점에서 낮은 염분을 보이는 1월에 높았으나, 정점 1은 용존 무기질소 농도는 낮았다. 김 엽체 색소 중 Chl a, PE 및 PC의 농도는 각각 $1,173{\sim}8,124{\mu}g\;DW\;g^{-1}$, $3,281{\sim}10,076{\mu}g\;DW\;g^{-1}$, $388{\sim}1,346{\mu}g\;DW\;g^{-1}$로서 조사 정점 2, 3에서 높은 농도를 보였다. 김 엽체의 색소농도는 지주식인 모무늬돌김과 방사무늬김이 혼재되어 양식되는 정점 1과 6보다 부류식인 방사무늬김만 생산되는 정점에서 높았고, 방사무늬김만 생산되는 정점에서는 정점 2와 3에서 높았다. 따라서, 김 엽체의 색소 농도는 양식 방법과 해양 환경적 요인에 의하여 달라질 수 있음을 알 수 있었다.