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http://dx.doi.org/10.4490/ALGAE.2003.18.4.321

Cryopreservation of Sporothalli of the Genus Porphyra (Bangiales, Rhodophyta) from Korea  

Jo, Young-Hyun
Kang, Sung-Pil
Seo, Tae-Ho
Choi, Sung-Je
Kho, Kang-Hee
Kuwano, Kazuyoshi
Saga, Naotsune
Kim, Min-Yong
Shin, Jong-Ahm
Publication Information
ALGAE / v.18, no.4, 2003 , pp. 321-331 More about this Journal
Abstract
Cryopreservation of sporothalli of the red alga Porphyra (P. seriata, P. yezoensis, P. tenera, P. pseudolinearis and P. dentata) was performed by the two-step cooling method in liquid nitrogen. The algal samples were suspended in various cryoprotective solutions, and slowly cooled to -40$^{\circ}C$ in 4 hours using a programmed freezer. After the first slow cooling the suspensions with cryoprotectants were immediately immersed in liquid nitrogen. The suspension from the programmed freezer was thawed quickly by agitation of the vial in a water bath at 40°C. When ice in the suspension of cryogenic vial was mostly melted, the vial was transferred to an ice bath for complete melting of the residual ice. The cryoprotectants in the vial were washed off by gradual dilution with seawater. The viability of the cell was assessed with neutral red staining. The viability of Porphyra samples ranged 54.6-70.9% when the mixed suspension of 10% dimethylsulfoxide and 0.5 M sorbitol in 50% seawater used as a cryoprotectant.
Keywords
cryopreservation; Porphyra; sporothallus; two-step cooling method;
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1 Fahy G.M., MacFarlane D.R, Angell CA. and Merymann H.T. 1984. Vitrification as an approach to cryopreservation. Cryobiology 21: 407-426   DOI   ScienceOn
2 Fenwick C. and Day J.G. 1992. Cryopreservation of Teiraselmis suecica cultured under different nutrients regimes. J. Appl. Phycol. 4: 105-109   DOI
3 Leeson E. A., Cann J. P. and Morris G. J. 1984. Maintenance bf algae and protozoa, In: Kirsop B. E. and Snell J,J.S. (eds), Maintenance of,Microorganisms. Academic Press, London. pp.131-160
4 Mazur P. 1984. Freezing of living cells: Mechanisms and implications. Am.J. Physiol. 247:C125-C142
5 Bajaj Y.P.S. and Reinert J. 1977. Cryobiology of plant cell cultures and establishment of gene-banks. In: Reinert J. and Bajaj y.P.5. (eds), Appliedandfundametal aspects of plant cell, tissue, and organ culture. Springer Verlag, Berlin. pp. 757-777
6 Ben-Amotz A. and Gilboa A. 1980a. Cryopreservation of marine unicellular algae. I. A survey of algae with regard to size, culture age, photosynthetic activity and chlorophyll-to-cell ratio. Mar. Ecol. Prog. Ser. 2: 157-161   DOI
7 Ben-Amotz A. and Gilboa A. 1980b. Cryopreservation of marine unicellular algae. II. Induction of freezing tolerance. Mar. Ecol. Prog. Ser. 2: 221-224   DOI
8 Chen T.H.H., Kartha K.K, Leung N.R, Kurz W.G.W., Chatson KB. and Constabel F. 1984b. Cryopreservation of alkaloid producing cell cultures of periwinkle (Catharanthus roseus). Plant Physiol. 75: 726-731   DOI   ScienceOn
9 Bousfield I.J. 1984. Maintenance of industrial and marine bacteria and bacteriophages, In: Kirsop B.E. and Snell J.J.S. (eds), Maintenance of microorganisms. Academic Press, New York. pp. 63-68
10 Chen T.H.H., Kartha K.K, Constabel F. and Gusta L.V. 1984a. Freezing characteristics of cultured Catharanthus roseus (L). G. Don cells treated with dimethylsulfoxide and sorbitol in relation to cryopreservation. PlantPhysiol. 75: 720-725   DOI   ScienceOn
11 Sugawara Y. and Sakai A. 1974.Survival of suspension cultured sycamore cells, cooled to the temperature of liquid nitrogen. PlantPhysiol. 54: 722-724   DOI   ScienceOn
12 Fujiyoshi E., Yamasaki M. and Kito H. 1993a. Cryopreservation of conchocelis of Porphyra yezoensis form. narawaensis. Suisanzoshoku 41: 85-87 (in Japanese with English abstract)
13 Fujiyoshi E., Yamasaki M., Umezawa S. and Kito H. 1993b. Prefreezing conditions for cropreservation of conchocelis of Porphyra yezoensis form. narawaensis. Suisanzoshoku 41: 547-551(in Japanese with English abstract)
14 Fujiyoshi E. 1997. Cryopreservation on Porphyra. Nat. Hist. Res. Special Issue No.3: 83-87
15 Kono S., Kuwano K, Ninomiya M., Onishi J. and Saga N. 1997. Cryopreservation of Enteromorpha intestinalis (Ulvales, Chlorophyta) in liquid nitrogen. Phycologia 36: 76-78   DOI
16 Kono S., Kuwano K. and Saga N. 1998. Cryopreservation of Eisenia bicyclis (Laminari ales, Phaeophyta) in liquid nitrogen.j. Mar. bioteph. 6: 220-223
17 Kuwano K and Saga N.,2000, Cryopreservation of marine algae: Applications in biotechnology. In: Fingerman M. and Nagabhushanam R. (eds), Recent advances in marine biotechnology. Volume 4: AqiiacuUure. Part A, Seaweeds and invertevrates, Science Publishers Inc., New Hampshire. pp. 23-40
18 Kuwano K, Aruga Y. and Saga N. 1994. Cryopreservation of the conchocelis of Porphyra (Rhodophyta) by applying a simple prefreezing system.J. Phycol. 30: 566-570   DOI   ScienceOn
19 Kuwano K, Aruga Yiand Saga N.1992. Preliminary study on cryopreservationof the .conchocelts of Porphyra yezoensis. Fish. Sci. 58: 1793-8   DOI
20 Kuwano K, Aruga Y. and Saga N. 1993. Cryopreservation of the conchocelis of the marine alga PorphY,k yekensis Ueda (Rhodophyta) in liquid nitrogen. Plant Sci: 94: 215-25   DOI   ScienceOn
21 Kuwano K, Aruga Y. and Saga N. 1996. Cryopreservation of the clonal gametophytic thalli of Porphyra (Rhodophyta). Plant Sci.116: 117-124   DOI   ScienceOn
22 Migita S. 1964. Freeze-preservation of Porphyra thalli in viable state-I. Bull. Fac. Fish. Nagasaki Univ. 17: 44-54 (in Japanese with English summary)
23 Migita S. 1966. Freeze-preservation of Porphyra thalli in viable state-H. Effect of cooling velocity and water content of thalli on the frost resistance. Bull. Fac. Fish. Nagasaki Univ. 21: 131-138(in Japanese with English abstract)
24 Migita S. 1967. Viability and spore-liberation of conchocelisphase, Porphyra ienera, freeze-preserved in seawater. Bull. Fac. Fish. Nagasaki Univ. 22: 33-43 (in Japanese with English abstract)
25 Miura A. 1992. Nori (Porphyra yezoensis). In: Miura A. (ed.), Cultivation of edible algae in Japan. Koseisha Koseikaku, Tokyo. pp. 11-24 (in Japanese)
26 Morris G.J. 1978. Cryopreservation of 250 strains of Chlorococcales by the method of two-step cooling. Br. Phycol. J. 13: 15-24   DOI   ScienceOn
27 Mumford T.F. and Miura A. 1988. Porphyra as food: cultivation and economics. In: Lembi C.A. and Waaland J.R, (eds), Algae and human affairs. Cambridge Univ, Press, Cambridge. pp. 87-117
28 Saga N., Sakanishi Y. and Ogishima T. 1989. Method for quick evaluation of cell viabillity in marine macroalgae. Jpn. J. Phycol. 129-136
29 Provasoli, L. 1966. Media and prospects for the cultivation of marine algae. In: Watanabe A. and Hattori A. (eds), Cultures and collections of algae, Proc. U.S. - Japan Conf., Hakone, September 1%6. Japan. Soc. Plant Physiol., Tokyo. pp,63-75
30 Renard P., Arbault S., Kaas R and Perez R 1992.A method for the cryopreservation of the gametophytes of the food algae Undaria pinnatifida (Laminariales). C.R. Acad. Sci. Serie (Paris) III. Sci. de la Vie 315: 445-451
31 Sakai A. and Sugawara Y. 1978. Survival of plant germlasm in liquid nitrogen. In: Li P. H. and Sakai A. (eds), Plant cold hardness andfreezing stress. Academic Press, New York. pp. 345-359
32 Sakanishi Y. and Saga N. 1994.Survival of female gametophytic cells of Laminaria diabolica Miyabe (Phaeophyta) in liquid nitrogen. Fish. Sci. 60: 623-624
33 Tsuchiya H. 1992.Cryopreservation of free-living conchocelis of Porphyra yezoensis -II. Cryoprotectants and freezing method. Bull. Chiba Pref. Fish. Exp. Sta. 50: 37-43 (in Japanese)
34 Tsuchiya H. 1994. Cryopreservation of free-living conchocelis of Porphyra yezoensis -III. Bull. Chiba Pref. Fish. Exp. Sta.52: 27-30 (in Japanese)
35 Ulrich J.M., Finkle B.J., Moore P.H. and Ginoza H. 1979. Effect of a mixture of cryoprotectants in attaining liquid nitrogen survival of callus cultures of a tropical plant. Cryobiology 16: 550-556   DOI   ScienceOn
36 van der Meer J.P. and Simpson F.J. 1984. Cryopreservation of Gracilaria tikvahiae (Rhodophyta) and other macrophytic marine algae. Phycologia 23: 195-20   DOI
37 Terumoto I. 1965. Freezing and drying in a red marnine alga, Porphyra yezoensis Ueda. LowTemp. Sci. Ser. B 23: 11-20 (in Japanese with English abstract)
38 Tsuchiya H. 1989.Cryopreservation of free-living conchocelis of Porphyra yezoensis. Bull. Chiba Pref. Fish. Exp. Sta. 47: 35-36 (in Japanese)