• The Journal of the Convergence on Culture Technology
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• v.6 no.4
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• pp.45-52
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• 2020

We examined the dyeability and antimicrobial activity of silks dyed singly with Sophora japonica L., Curcuma longa L., combination-dyed with Polygonum tinctorium L. and Sophora japonica L., and with Polygonum tinctorium L. and Curcuma longa L.. The color of silk dyed singly with Sophora japonica L., Curcuma longa L. was Yellow with Hue of 5.0Y and 0.6Y, respectively. Two types of combination-dyed silk were Blue Green with 2.6BG and 1.5BG, respectively. In the case of dry Cleaning fastness and rub fastness, all four types of dyed silk was good, achieving grade 4~5. The antimicrobial activity of Staphylococcus aureus of silk dyed with Sophora japonica L. extract showed excellent antibacterial activity of 99.5% and the other three types of dyed silk of 99.9%. In addition, in antibacterial activity against Klebsiella pneumonia, silk dyed with Sophora japonica L. extract showed 99.6%, and the other three types of dyed silk exhibited excellent antibacterial properties of 99.9%.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.spc1
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• pp.247-250
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• 2006

We herein studied the effect of polyamines (putrescine, spermidine and spermine) on growth and indigo biosynthesis in hairy root cultures of Polygonum tinctorium Lour. Our results revealed that polyamine treatment increased hairy root growth and indigo biosynthesisat all tested concentrations, with the highest growth rate (4.4 g/ flask) and indigo yield (216 ug/g) induced by 70 mg/L putrescine. These results show far the first time that the growth rates and indigo biosynthesis of Polygonum tinctorium hairy roots may be improved by addition of polyamines to the liquid culture medium.

• Journal of Applied Biological Chemistry
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• v.43 no.2
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• pp.109-111
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• 2000

Effect of the two-stage operation and cell concentration on indirubin production was investigated using bioreactor culture of Polygonum tinctorium. Two-stage culture was operated successfully for 110 days without any adverse effects on continuous indirubin production. Maximum indirubin concentration was found to be at 80 mg/bioreactor. Initial cell concentration significantly affected indirubin production. The indirubin production at 29.2% PCV was improved by 845%, compared to that at 5% PCV. For high-density bioreactor culture of P. tinctorium, a maximum production rate of 10.2 mg indirubin/L day was obtained. Indirubin recovery for bioreactor operation was also examined using XAD-2, XAD-4, XAD-7, and solid silicon. XAD-4 was 1.6-fold more effective than that for solid silicon in indirubin recovery.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2008.10a
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• pp.33-34
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• 2008

전통 쪽 염료인 니람 제조원리를 바탕으로 산업적인 쪽 염료 제조공정에 대한 제안과 함께 이와 관련한 몇몇 공정변수를 포함한다.

• Korean Journal of Medicinal Crop Science
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• v.3 no.2
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• pp.96-99
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• 1995

This experiment was carried out to analyze the effect of indole on the synthesis of indirubin in suspension culture of Polygonum tinctorium. Adding indole and L-tryptophan into culture media was re­vealed that indirubin was synthesized in callus grown on solid medium containing indole and proper concentration of indole for indirubin production was decided as 200mg/1. Indirubin content in suspension culture was higher than in solid medium with considerable amount of indirubin secresed into media in suspension culture and highest quantity of indirubin was obtained when indole was added into medium after 20 days suspension culture.

• Textile Coloration and Finishing
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• v.24 no.4
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• pp.253-259
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• 2012

In this work, the natural indigo production process from Polygonum tinctorium was balanced based on the traditional Niram method in Korea. A standard procedure was determined considering the conditions of indican extraction from plant material, the amount of alkali for precipitation, storage of extract, etc. The effect of experimental conditions on the yield of crude dye was investigated. The contents of indigo and indirubin of the crude dyes were analyzed by HPLC. Increase of the amount of crude dye was observed within 1-2.5 days of extraction time. Longer extraction beyond 2.5 days resulted in a slight decrease in the amount of crude dye. There was no consistency in terms of indigo content depending on extraction pH. We found that the storage of extract or harvested plants affected adversely to dye yield and dye quality. Based on the lab scale extraction, large scale extraction was performed for 2-2.5 days in water and 2.0-2.5 g/L of $Ca(OH)_2$ was applied for precipitation of indigo dye. We obtained natural indigo dye containing about 15% of pure indigo in scale-up production using whole plant except root.

• KSBB Journal
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• v.8 no.3
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• pp.193-199
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• 1993

To find out the optimum conditions for indigo cell culture in air-lift bioreactor, effects of media composition including nutrients and precursors of the indigo colorants on the cell growth and characteristics of the cell growth under various cultural conditions were analyzed. Optimum cultural conditions were tested and the growth characteristics were analyzed in external and internal loop type air-lift bioreactors during 14-day culture. Better cell growth was obtained when the inoculum size was higher in the range of 0.5∼2.5% packed cell volume tested. In the sucrose concentration of 2 to 4%, the cell growth was better when the sucrose concentration was 4% (w/w) in both types of reactors. Sucrose was used up in the early stage of exponential phase of growth At the optimum concentration of a Precursor tryptophan at 1 U UW was 3.8 g/l in internal loop bioreactor, and 3.5 g/l in external one after 14 days of cultivation. Addition of indole showed negative effect on cell growth of suspension culture in air-lift biorector culture and cell mass of 2.5 g/l and 2.2 g/l were obtained in external and internal loop bioreactor, respectively. Selected inorganic nitrogen source potassium nitrate showed about 110% increase in cell growth than that of control. DCW was 16.34 g/l under optimum conditions during 14-day cultivation in internal loop bioreactor.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.48 no.3
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• pp.57-65
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• 2016

Indigo (Polygonum tinctorium L.) is a typical blue dye which had been used from ancient times. This study was going to shade the complicated traditional methods extracting indigo dye by the fermentation and producing as adsorbate on calcium hydroxide, which says so called as the 'Indigo lime'. Accordingly we were going to make indigo through the hydrolysis of the hot water extractives of indigo leaves simply. During hot-water extraction, ${\beta}$-glucosidase which required hydrolysis of the linkage between indigo and glucose was not activated. To achieve this goal, indican was acid-hydrolyzed to glucose and indigo. The acetic acid, citric acid, hydrochloric acid, and sulfuric acid were used for the hydrolysis of hot water extractives. The hydrolysis conditions of extractives performed in water bath at $80^{\circ}C$ for 120 minutes and in an autoclave for 120 minutes. In the acid hydrolysis of extracted indican by hot water, the indican yields of acetic acid and hydrochloric acid hydrolysis were higher than sulfuric acid in water bath. Also, the indican yield of hydrochloric acid hydrolysis was better than sulfuric acid in autoclave. The hot water extracted indican was confirmed by HPLC analysis and its structure was confirmed by UV-Vis and FT-IR spectroscopy, compared with isolated indigo and commercial synthesized indigo. This improved extraction and hydrolysis methods can be replace the traditional indigo making method.

• Journal of the Korean Society of Clothing and Textiles
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• v.32 no.8
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• pp.1286-1298
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• 2008

This study was attempted to examine the indigo dyeing technique in the latter Joseon Dynasty, and to find diachronic specifics in the indigo dyeing method from 17C to 19C. The results are as follows: First, There were 2 kinds of indigo species-Yoram(Polygonum tinctorium Lour.) and Sungram(Isatis tinctoria L.)-in the latter Joseon Dynasty. Dangram was preferred in particular among Yoram species because of its high dyeing efficiency. Sungram was mainly used for making Jeon(indigo sediment). Second, Yoram cultivation took the following order: Sowing in April${\rightarrow}$planting out in June${\rightarrow}$harvesting leaves in August${\rightarrow}$collecting seeds in September. Sungram had more harvesting times and wider harvesting period than Yoram. Third, all indigo dyeing methods were grouped into two categories, one was temporary dyeing method, the other was permanent dyeing method. Mixture dyeing of the boiled and the raw, raw leaves dyeing, and fermentation dyeing belonged to the temporary dyeing. Jeon dyeing and Gaeogi dyeing belonged to the permanent dyeing. Finally, diachronic specifics from 17C to 19C were as follows: Decline in the mixture dyeing of the boiled and the raw, development in the raw leaves dyeing, continuance of the fermentation dyeing, and naturalization of Jeon dyeing technique.

• Journal of Life Science
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• v.29 no.1
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• pp.52-59
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• 2019

The leave of Polygonum tinctorium (LPT) have been used for centuries as a traditional medicine and as a food ingredient and natural dye. The aim of the current study was to develop high-value added products using LPT. Hot water extract (HWE) and ethanol extract (EE) of LPT were prepared, respectively, and their bioactivity was evaluated. The extraction ratio for the HWE was 27.6%, which was two-fold higher than that of the EE. The contents of total polyphenol in the HWE and total sugar in the EE were 51.2 mg/g and 297.8 mg/g, respectively. The total flavonoid and reducing sugar contents were similar in the extracts, irrespective of the extraction solvent. The HWE did not show antimicrobial activity in a disc-diffusion assay, but the EE showed strong growth inhibition against gram-positive bacteria. The EE exhibited stronger DPPH and ABTS radical scavenging activities and reducing power than those of the HWE. The HWE was particularly effective as a scavenger of nitrite ($RC_{50}$ of $6.0{\mu}g/ml$). In an antithrombosis activity assay, the EE showed significant anticoagulation activity as determined by an extended blood coagulation time (thrombin time, prothrombin time, and activated partial thromboplastin time), in addition to platelet aggregation activity. The HWE also showed platelet aggregation inhibitory activity. This report provides the first evidence of antithrombosis activities of LPT. Our results suggest that LPT has potential as a new antioxidant and antithrombosis agent.