• Journal of Life Science
• /
• v.21 no.10
• /
• pp.1401-1406
• /
• 2011

Morphological changes in immune cells occur due to pathogen infection and natural circulation. T cells produce uropod, filopodia, lamellipodia, and microvilli for inflammation, immunosurvelliance, migration, and diapedesis. Short finger-like microvilli cover the surfaces of circulating mammalian immune cells. The surface features of monocytes and neutrophils are quite different, containing membrane ruffles as their predominant structure. In this study, we present the involvement of actin cytoskeleton regarding T lymphocyte microvilli. From analysis of scanning electron micrographs, Jurkat T lymphocyte microvilli was observed to rapidly disassemble when exposed to the actin-sequestering molecule, cytochalasin D. In contrast to cytochalasin D treatment, we found that median microvillar thickness was enlarged on Jurkat T lymphocytes treated with PMA via Lin-11, Isl-1, Mec-3 Kinase (LIMK) and cofilin signaling. In addition, actin cytoskeleton was involved in polarity formation in EL4 T lymphocytes. These results suggest that microvilli formation or polarity of T lymphocytes are involved in actin cytoskeleton dynamics.

• Journal of Chest Surgery
• /
• v.24 no.11
• /
• pp.1081-1089
• /
• 1991

Atrial fibrillation is characterized by beat to beat irregularity of shape, size, conduction time and polarity. The mechanism of atrial fibrillation can be explained by so called "Multiple wavelet theory". The adverse effect of atrial fibrillation is the decrease of cardiac output by absence of atrial kick[10 ~ 15%] and the possibility of thrombosis in the left atrium which is dangerous to develop the thromboembolism is increased. The present study was designed to assess the effect of overdrive pacing on the suppression of recurring of atrial fibrillation after open heart surgery and the results were summarized as follows: 1. There were no significant differences of factors between converting patients and non-converting patients to normal sinus rhythm by electric cardioversion after open heart surgery. 2. Among converting patients to normal sinus rhythm, there were no significant differences of factors between study group and control group. 3. Cardiothoracic Ratio on the preoperative chest film was significantly larger in the patients of recurring atrial fibrillation within 72hrs than in the patients of maintaining normal sinus rhythm. [61.7$\pm$ 1.4% vs 67.7$\pm$2.4%, p=0.03] 4. There was a significant difference of suppressive effects between overdrive pacing group and control group among recurred cases until 24, 48, and 72hours [Fisher`s exact test ; p=0.037, p=0.076, p=0.53, respectively] 5. There was a difference of the delay of recurring of atrial fibrillation between study group and control group among recurred cases within 72 hours.[53.4$\pm$6.9hr vs. 19.3$\pm$3.8 hr, p<0.01] We think that the overdrive pacing may suppress the natural pacemaker and the converted normal sinus rhythm is maintained longer than control group during critical immediate postoperative period.ve period.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2013.05a
• /
• pp.382-384
• /
• 2013

LED is much brighter than the traditional incandescent, and low power consumption. Development and commercialization of LED fluorescent lamp is made in order to improve efficient fluorescent lamps with this existing and environmental problems. Because, unlike fluorescent lamps, it is not possible to directly use AC power to the unidirectional element having a polarity, LED requires a power supply. The power supply function is lowered as compared with the fluorescent lamp common in terms of maintenance costs because of the high price large. In this study, we constructed a circuit that can be controlled with a constant current in order to eliminate a phenomenon that brightness of the light which rectifies the AC power supply easy to place in the power supply due to the current change due to the voltage fluctuation is changed, in maintenance, I would like to propose how to develop a device which can be easily replaced less expensive composed of interchangeable modules of Plug in / out method to distinguish the LED module this.

• 제어로봇시스템학회:학술대회논문집
• /
• 2003.10a
• /
• pp.1749-1753
• /
• 2003

We proposed several systems for 1Giga bit Modem. The first, Binary ASK(Amplitude Shift Keying) system has a high speed shutter transmitter and no IF(Intermediate Frequency) receiver only by symbol synchronization. The advantage of proposed system is that circuitry is very simple without IF process. The disadvantage of proposed system are that line spectrum occurs interference to other channels, and enhancement to 4-level system is impossible due to its large SNR degradation. The second, Binary phase modulation system has a high speed shutter transmitter and IF-VCO(IF-Voltage Controlled Oscillator) control by base-band phase rotation. Polarity of shutter window is changed by the binary data. The window should be narrow same as above ASK. The advantage of proposed system is which error rate performance is superior. The disadvantage of proposed system are that Circuitry is more complex, narrow pull-in range of receiver caused by VCO and spectrum divergence by the non-linear amplifier. The third, 4-QAM(Quadrature Amplitude Modulation)system has a nyquist pulse transmitter and IF-VCO control by symbol clock. The advantage of proposed system are that signal frequency band is a half of 1GHz, reliable pull-in of VCO and possibility of double speed transmission(2Gbps) by keeping 1GHz frequency-band. The disadvantage of proposed system are that circuit complexity of pulse shaping and spectrum divergence by the non-linear amplifier.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.21 no.4
• /
• pp.984-995
• /
• 1996

Data errors according to the various noises caused in the satellite communication links are corrected by the Viterbi decoding algorithm which has extreme error correcting capability. In this paper, we designed and implemented a convolutional encoder and Viterbi decoder ASIC which is used to encode the input data at the transmit side and correct the errors of the received data at the receive side for use in the VSAT communication system. And this chip may be used in any BPSK, QPSK, or OQPSK transmission system. The ambiguity resolver corrects PSK modem ambiguities by delaying, interting, and/or exchanging code symbol to restore their original sequence and polarity. In case of previous decoding system, ambiguity state(AS) of data is resolved by external control logic and extra redundancy data are needed to resolve AS. But, by adopting decoder proposed in this paper, As of data is resolved automatically by internal logic of decoder in case of continuous mode, and by external As line withoug extra redudancy data in burst mode case. So, decoding parts are simple in continuous mode and transmission efficiency is increased in bust mode. The features of this chip are full duplex operation with independent transmit and receive control and clocks, start/stop inputs for use in burst mode systems, loopback function to verify encoder and decoder, and internal or external control to resolve ambinguity state. For verification of the function and performance of a fabricated ASIC chip, we equiped this chip in the Central and Remote Earth Station of VSAT system, and did the performance test using the commerical INTELSAT VII under the real satellite link environmens. The results of test were demonstrated the superiority of performance.

• Molecules and Cells
• /
• v.37 no.4
• /
• pp.307-313
• /
• 2014

Cell migration requires a defined cell polarity which is formed by diverse cytoskeletal components differentially localized to the poles of cells to extracellular signals. Rap-GAP3 transiently and rapidly translocates to the cell cortex in response to chemoattractant stimulation and localizes to the leading edge of migrating cells. Here, we examined localization of truncated RapGAP3 proteins and found that the I/LWEQ domain in the central region of RapGAP3 was sufficient for posterior localization in migrating cells, as opposed to leading-edge localization of full-length Rap-GAP3. All truncated proteins accumulated at the leading edge of migrating cells exhibited clear translocation to the cell cortex in response to stimulation, whereas proteins localized to the posterior in migrating cells displayed no translocation to the cortex. The I/LWEQ domain appears to passively accumulate at the posterior region in migrating cells due to exclusion from the extended front region in response to chemoattractant stimulation rather than actively being localized to the back of cells. Our results suggest that posterior localization of the I/LWEQ domain of RapGAP3 is likely related to F-actin, which has probably different properties compared to newly formed F-actin at the leading edge of migrating cells, at the lateral and posterior regions of the cell.

• Mass Spectrometry Letters
• /
• v.8 no.2
• /
• pp.39-43
• /
• 2017

Meldonium is a drug for treating ischemia by expanding the arteries but it can also enhance the performance of sports players. The World Anti-Doping Agency (WADA) has included it in the list of prohibited substances since 2016. Meldonium is one of the challenging substances for anti-doping testing because it is difficult to recover by general liquid-liquid or solid phase extraction due to its permanent charge and high polarity. Therefore, high-performance liquid chromatography (HPLC) is currently used by injecting a diluted urine sample (known as the "dilute-and-shoot" strategy). There is no loss of target compounds in the extraction/cleanup procedure but its high matrix effect could interfere in their separation or detection from the endogenous urinary compounds. We report a single method using high-resolution mass spectrometry that can be used for both screening and confirmation, which follows the "dilute-and-shoot" strategy. In this method, the endogenous compounds' interfering peaks in the mass spectrum are separated at a high resolution of FWHM 140,000, and the results are suitable for substance detection following the WADA guidelines. The interferences in the obtained mass spectrum of the urine matrix are identified as acetylcholine, lysine, and glutamine by further analysis and database searching. Validation of the method is performed in routine anti-doping testing, and the limit of detection is 50 ng/mL. This method uses simple sample preparation and a general reverse phase HPLC column, and it can be easily applied to other substances.

• Proceedings of the KWS Conference
• /
• 2002.10a
• /
• pp.107-112
• /
• 2002

Ferritic stainless steels would be the most important alloys under the chloride environment. They are a cheaper alternative to austenitic stainless steels [1]. The present study is related to gas tungsten arc welding (GTAW) characteristics of Type 444 stainless steels. The heat of welding leads to grain coarsening in the HAZ and in the weld metal of ferritic stainless steels because they solidify directly from the liquid to the ferritc phase without any intermediate phase transformation. It is therefore recommended that these alloys be welded with a low heat input and at high welding speeds. Attempts to improve weldability were made by using of direct current straight polarity (DCSP) and pulsed current GTAW processes in this study. Measuring weld bead, grain size and Erichsen test were performed and the effects of heat input, pulse frequency on the weld metal and HAZ were studied. The main results were obtained as followings: decreasing heat input was effective to control the width of weld both in DCSP welding and in pulsed current welding; pulsed current welding was found to refine the grain size effectively and the finest grain size was found at the frequency of 150Hz in pulsed current welding; it was found that decreasing heat input also refine the HAZs effectively and the frequency had no different effect on HAZ at the same heat input; the ductility could be improved effectively in pulsed current welding.

• Bulletin of the Korean Chemical Society
• /
• v.34 no.3
• /
• pp.837-850
• /
• 2013

Bovine viral diarrhea virus (BVDV), a major pathogen of cattle, is a well-characterized pestivirus which has been used as a good model virus for HCV. The RNA-dependent RNA polymerase (RdRp) plays a key role in the RNA replication process, thus it has been targeted for antivirus drugs. We employed two-dimensional quantitative structure-activity relationship (2D-QSAR) and molecular field analysis (MFA) to identify the molecular substructure requirements, and the particular characteristics resulted in increased inhibitory activity for the known series of compounds to act as effective BVDV inhibitors. The 2D-QSAR study provided the rationale concept for changes in the structure to have more potent analogs focused on the class of arylazoenamines, benzimidazoles, and acridine derivatives with an optimal subset of descriptors, which have significantly contributed to overall anti-BVDV activity. MFA represented the molecular patterns responsible for the actions of antiviral compound at their receptors. We conclude that the polarity and the polarizability of a molecule play a main role in the inhibitory activity of BVDV inhibitors in the QSAR modeling.

• Korean Journal of Pharmacognosy
• /
• v.38 no.1
• /
• pp.95-99
• /
• 2007

In order to find novel cancer chemopreventive agents, quinone reductase (QR) inductive activity of methanol extract of herbal medicines was examined using murine hepatoma, hepa1c1c7 cells. QR has been used as an anticarcinogenic marker enzyme in cancer chemoprevention study. The methanol extract of Alpinia officinarum (Zingiberace) showed showed significantly strong quinone reductase inductive activity compared to the control group. The methanol extract of Alpinia officinarum was successively fractionated with various solvents according to polarity. Hexane, ethyl acetate, buthanol and water fractions were obtained and theirs activities were assessed. The QR inductive effect was moved to the ethyl acetate fraction and was highly increased. The CD (concentration required to double the specific activity of QR) value of ethyl acetate fraction was 8.6 ${\mu}g/mL$. Alpinia officinarum also showed strong antioxidant activity. These results suggest that Alpinia officinarum can be developed as cancer chemopreventive agent.